Volume 28, Issue 6 (1-2026)
J Arak Uni Med Sci 2026, 28(6): 485-493 |
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Parastesh M, Yasavoli Sharahi A, Moradi J, Aria B. Comparison of the Effects of Two Selected Training Protocols on Brain-Derived Neurotrophic Factor, Working Memory, and Aerobic/Anaerobic power in Adolescent Futsal Players. J Arak Uni Med Sci 2026; 28 (6) :485-493
URL: http://jams.arakmu.ac.ir/article-1-7991-en.html
URL: http://jams.arakmu.ac.ir/article-1-7991-en.html
1- Department of Sports Physiology, Faculty of Sports Sciences, Research Institute of Applied Studies of Sports Sciences, Arak University, Arak, Iran , M-parastesh@Araku.ac.ir
2- Department of Sports Physiology, Faculty of Sports Sciences, Arak University, Arak, Iran
3- Department of Motor Behavior, Motor learning, Research Institute of Applied Studies of Sports Sciences, Motor Development, Sport Psychology, Arak University, Arak, Iran
4- Department of Physical Education and Sports Sciences, Faculty of Psychology and Educational Sciences, Yazd University, Yazd, Iran
2- Department of Sports Physiology, Faculty of Sports Sciences, Arak University, Arak, Iran
3- Department of Motor Behavior, Motor learning, Research Institute of Applied Studies of Sports Sciences, Motor Development, Sport Psychology, Arak University, Arak, Iran
4- Department of Physical Education and Sports Sciences, Faculty of Psychology and Educational Sciences, Yazd University, Yazd, Iran
Abstract: (718 Views)
Introduction: Physical and cognitive fitness are crucial in futsal, making the identification of effective training methods essential. This study aimed to investigate the effects of two high-intensity interval training (HIIT) protocols on plasma brain-derived neurotrophic factor (BDNF) levels, working memory, and selected physical fitness factors (aerobic and anaerobic capacity) in adolescent futsal players.
Methods: This semi-experimental study was conducted on 15–16-year-old futsal players from Arak city with at least three years of club experience (approved by Arak University Ethics Committee, code: IR.ARAKU.RCE.1401.027). Twenty-four participants were randomly assigned to HIIT1 (10×1-minute intervals with 1-minute rest) or HIIT2 (3×4-minute intervals with 2-minute rest) groups, training for eight weeks. Aerobic capacity, anaerobic capacity, plasma BDNF levels, and working memory were assessed pre- and post-intervention. Data were analyzed using Paired and Independent T-tests (P <0.05).
Results: The HIIT1 group showed significant improvements in aerobic capacity (P = 0.001) and BDNF levels (P = 0.001). Similarly, HIIT2 demonstrated significant increases in aerobic capacity (P = 0.001), anaerobic capacity (P = 0.014), and BDNF levels (P = 0.001). Working memory showed no significant changes in either group (P = 0.780), with no between-group differences observed.
Conclusions: Both HIIT protocols significantly improved aerobic capacity and BDNF levels, while only HIIT2 enhanced anaerobic capacity. Neither protocol affected working memory. HIIT appears effective for enhancing physiological and physical fitness factors in adolescent futsal players.
Methods: This semi-experimental study was conducted on 15–16-year-old futsal players from Arak city with at least three years of club experience (approved by Arak University Ethics Committee, code: IR.ARAKU.RCE.1401.027). Twenty-four participants were randomly assigned to HIIT1 (10×1-minute intervals with 1-minute rest) or HIIT2 (3×4-minute intervals with 2-minute rest) groups, training for eight weeks. Aerobic capacity, anaerobic capacity, plasma BDNF levels, and working memory were assessed pre- and post-intervention. Data were analyzed using Paired and Independent T-tests (P <0.05).
Results: The HIIT1 group showed significant improvements in aerobic capacity (P = 0.001) and BDNF levels (P = 0.001). Similarly, HIIT2 demonstrated significant increases in aerobic capacity (P = 0.001), anaerobic capacity (P = 0.014), and BDNF levels (P = 0.001). Working memory showed no significant changes in either group (P = 0.780), with no between-group differences observed.
Conclusions: Both HIIT protocols significantly improved aerobic capacity and BDNF levels, while only HIIT2 enhanced anaerobic capacity. Neither protocol affected working memory. HIIT appears effective for enhancing physiological and physical fitness factors in adolescent futsal players.
Keywords: Short-term high-intensity interval training, Long-term high-intensity interval training, BDNF, Working memory, Aerobic capacity, Anaerobic capacity
References
1. Wu C, Xu Y, Chen Z, Cao Y, Yu K, Huang C. The Effect of Intensity, Frequency, Duration and Volume of Physical Activity in Children and Adolescents on Skeletal Muscle Fitness: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. Int J Environ Res Public Health. 2021;18(18):9640. pmid: 34574565 doi: 10.3390/ijerph18189640
2. Patel H, Alkhawam H, Madanieh R, Shah N, Kosmas CE, Vittorio TJ. Aerobic vs anaerobic exercise training effects on the cardiovascular system. World J Cardiol. 2017;9(2):134-8. pmid: 28289526 doi: 10.4330/wjc.v9.i2.134
3. Elhamalawy A. Review of the pros and cons when comparing high intensity interval training to moderate intensity continuous training. International Journal of Research in Medical Sciences. 2024;12(2):630-3. doi:10.18203/2320-6012.ijrms20240243
4. Ito S. High-intensity interval training for health benefits and care of cardiac diseases-the key to an efficient exercise protocol. World J Cardiol. 2019;11(7):171-88. pmid: 31565193 doi: 10.4330/wjc.v11.i7.171
5. Coates AM, Joyner MJ, Little JP, Jones AM, Gibala MJ. A perspective on high-intensity interval training for performance and health. Sports Med. 2023;53(Suppl 1):85-96. pmid: 37804419 doi: 10.1007/s40279-023-01938-6.
6. Jahangiri M, Shahrbanian S, Gharakhanlou R. High intensity interval training alters gene expression linked to mitochondrial biogenesis and dynamics in high fat diet fed rats. Sci Rep. 2025;15(1):5442. pmid: 39952980 doi: 10.1038/s41598-025-86767-5
7. Duval C, Rouillier M-A, Rabasa-Lhoret R, Karelis AD. High intensity exercise: Can it protect you from a fast food diet? Nutrients. 2017;9(9):943. pmid: 28846611 doi: 10.3390/nu9090943
8. Liu K, Zhao W, Li C, Tian Y, Wang L, Zhong J, et al. The effects of high-intensity interval training on cognitive performance: a systematic review and meta-analysis. Sci Rep. 2024;14(1):32082. pmid: 39738783 doi: 10.1038/s41598-024-83802-9
9. Miranda M, Morici JF, Zanoni MB, Bekinschtein P. Brain-derived neurotrophic factor: a key molecule for memory in the healthy and the pathological brain. Front Cell Neurosci. 2019;13:472800. pmid: 31440144 doi: 10.3389/fncel.2019.00363
10. Murawska-Ciałowicz E, de Assis GG, Clemente FM, Feito Y, Stastny P, Zuwała-Jagiełło J, et al. Effect of four different forms of high intensity training on BDNF response to Wingate and Graded Exercise Test. Sci Rep. 2021;11(1):8599. doi:10.1038/s41598-021-88069-y
11. Romero Garavito A, Díaz Martínez V, Juárez Cortés E, Negrete Díaz JV, Montilla Rodríguez LM. Impact of physical exercise on the regulation of brain-derived neurotrophic factor in people with neurodegenerative diseases. Front Neurol. 2025;15:1505879. pmid: 39935805 doi: 10.3389/fneur.2024.1505879
12. Chai WJ, Abd Hamid AI, Abdullah JM. Working memory from the psychological and neurosciences perspectives: a review. Front Psychol. 2018;9:401. pmid: 29636715 doi: 10.3389/fpsyg.2018.00401
13. Chaire A, Becke A, Düzel E. Effects of physical exercise on working memory and attention-related neural oscillations. Front Neurosci. 2020;14:239. pmid: 32296302 doi: 10.3389/fnins.2020.00239
14. de Lima NS, De Sousa RAL, Amorim FT, Gripp F, Diniz e Magalhaes CO, Henrique Pinto S, et al. Moderate-intensity continuous training and high-intensity interval training improve cognition, and BDNF levels of middle-aged overweight men. Metab Brain Dis. 2022;37(2):463-71. pmid: 34762211 doi: 10.1007/s11011-021-00859-5
15. Azuma K, Osawa Y, Tabata S, Horisawa S, Katsukawa F, Ishida H, et al. Association of serum BDNF concentration with high-intensity interval training. Japanese Journal of Physical Fitness and Sports Medicine. 2015;64(2):227-32. doi:10.7600/jspfsm.64.227
16. Seifert T, Brassard P, Wissenberg M, Rasmussen P, Nordby P, Stallknecht B, et al. Endurance training enhances BDNF release from the human brain. Am J Physiol Regul Integr Comp Physiol. 2010;298(2):R372-R7. pmid: 19923361 doi: 10.1152/ajpregu.00525.2009
17. Milioni F, Millet GY, de Poli RAB, Motta Pinheiro Brisola G, de Souza Malta E, Eduardo Redkva P, et al. Effects of 4-week high intensity interval training on anaerobic capacity, repeated-sprints performance and neuromuscular function. Sport Sci Health. 2024;20(3):1109-18. doi: 10.1007/s11332-024-01214-8
18. Kuswoyo DD, Lahinda J. The effects of high-intensity interval training (HIIT) in improving VO2 max football student activity unit, University of Musamus. Enfermería Clínica. 2020;30:507-11. doi: 10.1016/j.enfcli.2019.10.130
19. Veale JP, Pearce AJ, Carlson JS. The Yo-Yo intermittent recovery test (level 1) to discriminate elite junior Australian football players. J Sci Med Sport. 2010;13(3):329-31. pmid: 19451033 doi: 10.1016/j.jsams.2009.03.006
20. Bradley PS, Mascio MD, Bangsbo J, Krustrup P. The maximal and sub-maximal versions of the Yo-Yo intermittent endurance test level 2 are simply reproducible, sensitive and valid. Eur J Appl Physiol. 2012;112(5):1973-5. pmid: 21927831 doi: 10.1007/s00421-011-2155-1
21. Eskandari K, Bigdeli I, Mohammad Rezaei A, Fadaei A. Comparison of Working Memory and Response Inhibition in Methamphetamine-Dependent Individuals and Normal Individuals [in Persian]. Clinical Psychology Studies. 2016;6(21):62-74. doi: 10.22054/jcps.2016.2384
22. Harrison AG, Beal AL, Armstrong IT, Gallagher A. Measurement of Working Memory on the Wechsler Adult Intelligence Scale: Should We Subtract Arithmetic? Psychol. Inj. and Law. 2024;17(1):55-65. doi: 10.1007/s12207-024-09499-3
23. Atakan MM, Li Y, Koşar ŞN, Turnagöl HH, Yan X. Evidence-based effects of high-intensity interval training on exercise capacity and health: A review with historical perspective. Int J Environ Res Public Health. 2021;18(13):7201. pmid: 34281138 doi: 10.3390/ijerph18137201
24. Küçük H, Soyler M, Ceylan T, Ceylan L, ŞAHİN F. Effects of acute and chronic high-intensity interval training on serum irisin, BDNF and apelin levels in male soccer referees. Journal of Men's Health. 2024;20(2):120-5. doi:10.22514/jomh.2024.027
25. Boyne P, Meyrose C, Westover J, Whitesel D, Hatter K, Reisman DS, et al. Exercise intensity affects acute neurotrophic and neurophysiological responses poststroke. J Appl Physiol (1985). 2019;126(2):431-43. pmid: 30571289 doi: 10.1152/japplphysiol.00594.2018
26. Donato K, Ceccarini MR, Dhuli K, Bonetti G, Medori MC, Marceddu G, et al. Gene variants in eating disorders. Focus on anorexia nervosa, bulimia nervosa, and binge-eating disorder. J Prev Med Hyg. 2022;63(2 Suppl 3):E297. pmid: 36479493 doi: 10.15167/2421-4248/jpmh2022.63.2S3.2772
27. Hsu C-C, Fu T-C, Huang S-C, Chen CP-C, Wang J-S. Increased serum brain-derived neurotrophic factor with high-intensity interval training in stroke patients: a randomized controlled trial. Ann Phys Rehabil Med. 2021;64(4):101385. pmid: 32344098 doi: 10.1016/j.rehab.2020.03.010
28. Li P, Hu Y, Tong L, Bi X. High-intensity training on CREB activation for improving brain health: a narrative review of possible molecular talks. Front Endocrinol (Lausanne). 2025;15:1498495. pmid: 39902166 doi: 10.3389/fendo.2024.1498495
29. Jiménez-Maldonado A, Rentería I, García-Suárez PC, Moncada-Jiménez J, Freire-Royes LF. The impact of high-intensity interval training on brain derived neurotrophic factor in brain: a mini-review. Front Neurosci. 2018;12:839. pmid: 30487731 doi: 10.3389/fnins.2018.00839.
30. Qi J-y, Yang L-k, Wang X-s, Wang M, Li X-b, Feng B, et al. Mechanism of CNS regulation by irisin, a multifunctional protein. Brain Res Bull. 2022;188:11-20. pmid: 35850187 doi: 10.1016/j.brainresbull.2022.07.007.
31. Yuan S, Lin L, Liu L, Zhang X, Gu Q. A comparison of the acute effects of high intensity interval training and moderate intensity continuous training on working memory and emotional state in adolescent women with subthreshold depression. Front Public Health. 2025;13:1505959. pmid: 40008151 doi: 10.3389/fpubh.2025.1505959
32. Alves AR, Dias R, Neiva HP, Marinho DA, Marques MC, Sousa AC, et al. High-intensity interval training upon cognitive and psychological outcomes in youth: A systematic review. Int J Environ Res Public Health. 2021;18(10):5344. pmid: 34067861 doi: 10.3390/ijerph18105344
33. De la Rosa A, Solana E, Corpas R, Bartrés-Faz D, Pallàs M, Vina J, et al. Long-term exercise training improves memory in middle-aged men and modulates peripheral levels of BDNF and Cathepsin B. Sci Rep. 2019;9(1):3337. pmid: 30833610 doi: 10.1038/s41598-019-40040-8
34. Sebastião AM, Assaife-Lopes N, Diógenes MJ, Vaz SH, Ribeiro JA. Modulation of brain-derived neurotrophic factor (BDNF) actions in the nervous system by adenosine A2A receptors and the role of lipid rafts. Biochim Biophys Acta. 2011;1808(5):1340-9. pmid: 20603099 doi: 10.1016/j.bbamem.2010.06.028.
35. Blackmore DG, Schaumberg MA, Ziaei M, Belford S, To XV, O’Keeffe I, et al. Long-term improvement in hippocampal-dependent learning ability in healthy, aged individuals following high intensity interval training. Aging Dis. 2024;16(3):1732-54. pmid: 39012673 doi: 10.14336/AD.2024.0642
36. Konrad K, Firk C, Uhlhaas PJ. Brain development during adolescence: neuroscientific insights into this developmental period. Dtsch Arztebl Int. 2013;110(25):425-31. pmid: 23840287 doi: 10.3238/arztebl.2013.0425
37. Silva R, Damasceno M, Cruz R, Silva-Cavalcante M, Lima-Silva AE, Bishop D, et al. Effects of a 4-week high-intensity interval training on pacing during 5-km running trial. Braz J Med Biol Res. 2017;50(12):e6335. pmid: 29069224 doi: 10.1590/1414-431X20176335
38. Stöggl TL, Björklund G. High intensity interval training leads to greater improvements in acute heart rate recovery and anaerobic power as high volume low intensity training. Front Physiol. 2017;8: 562. pmid: 28824457 doi: 10.3389/fphys.2017.00562.
39. Lee M-C, Chung Y-C, Thenaka PC, Wang Y-W, Lin Y-L, Kan N-W. Effects of different HIIT protocols on exercise performance, metabolic adaptation, and fat loss in middle-aged and older adults with overweight. Int J Med Sci. 2024;21(9):1689-700. pmid: 39006847 doi: 10.7150/ijms.96073.
40. Farland CV, Schuette J, Foster C, Porcari JP, Doberstein ST, Harbin M, et al. The effects of high intensity interval training versus steady state training on aerobic and anaerobic capacity. Medicine & Science in Sports & Exercise. 2015;47(5S):133.
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