Volume 19, Issue 11 (2-2017)                   J Arak Uni Med Sci 2017, 19(11): 39-46 | Back to browse issues page

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Zolfaghari M, Khansarinejad B, Ganji A, Hamzehloo Z, Abtahi H. Frequency Determination of Ureaplasma and Mycoplasma Genitalium Species in Female with Vaginitis Infection using Real- Time PCR. J Arak Uni Med Sci 2017; 19 (11) :39-46
URL: http://jams.arakmu.ac.ir/article-1-4611-en.html
1- Department of Immunology and Microbiology, Arak University of Medical Sciences, Arak, Iran
2- PhD in Medical Virology, Department of Microbiology and Immunology, Arak University of Medical Sciences, Arak, Iran
3- PhD in Medical Immunology, Department of Microbiology and Immunology, Arak University of Medical Sciences, Arak, Iran
4- Department of Medical Biotechnology, Arak University of Medical Sciences, Arak, Iran
5- PhD in Medical Microbiology, Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran , abtahi@arakmu.ac.ir
Abstract:   (6750 Views)

Abstract

Background: Ureaplasma and M. genitalium species belong to a kind of bacteria that are sexually transmitted and are the possible cause of pelvic inflammatory disease and nongonococcal urethritis, and et al. The aim of this study was to determine the urea plasma and Mycoplasma genitalium species frequency in women with vaginal infection and various sexual partners who referred to women, s health promotion and treatment center in Arak.

Materials and Methods: Endocervical swab samples from 110 women with vaginal infections referred to womens health promotion and treatment center in Arak, were prepared. Patients’ personal information and identities during reception process were registered. The samples were transferred to the laboratory in the transport environment and after DNA extraction, were evaluated according to Real-time PCR assay.

Results: Urea plasma and Mycoplasma genitalium bacteria existed in 96(87.27%) and 4(3.63%) of patients, respectively. Among them, 4 cases had both bacteria infections. The amount of isolation in young women between 30-39 years old was more than others.

Conclusion: The results show that the colonization of urea plasma species in adult women is 40-80% and in studied group is 87.27%. These results indicate that with due attention to the increasing number of sexual partners and the increase of sexual activity, the urea plasma colonization of women will increase. In view of the potential influence of mycoplasma species on side effects resulted from pregnancy infection of mothers and mortality, on-time diagnosis and treatment will be increasingly essential.

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Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2016/08/20 | Accepted: 2016/11/12

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