fa بررسی بیان ژن فاکتور رشد اندوتلیال عروقی مرتبط با عروق زایی و اینترلوکین-10 در مدل تجربی تومور پستان علوم پایه Basic Sciences پژوهشي اصیل Original Atricle <span style="font-size:11pt"><span style="line-height:101%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">مقدمه:</span></span></span></span></b><span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black"> سرطان پستان، از جمله شایع‌ترین سرطان‌ها و علت اصلی مرگ و میر در زنان می‌باشد. یکی از مکانیسم‌های سلول‌های سرطانی برای عدم دسترسی سیستم ایمنی به آنها، تولید ترکیبات سرکوب‌کننده از قبیل اینترلوکین-10 است. از طرف دیگر</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">فاکتور</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">رشد</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">اندوتلیال</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">عروقی</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">با</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">اتصال</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">به</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">گیرنده‌های</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">خود</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">در</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">سطح</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">سلول‌های</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">اندوتلیال نقش</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">مهمی</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">در</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">نفوذپذیری</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">عروق</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">و</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">رگ‌زایی</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">تومور</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">ایفا</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black">می‌کند. در این مطالعه بیان ژن اینترلوکین-10 و فاکتور رشد اندوتلیال عروقی در بافت تومور پستان در مدل تجربی تومور بررسی شد. </span></span></span></span><span lang="FA" style="line-height:101%"><span b="" nazanin="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:104%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:104%"><span b="" nazanin="" style="font-family:"><span style="color:black">روش کار:</span></span></span></span></b><span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:104%"><span b="" nazanin="" style="font-family:"><span style="color:black"> ابتدا در موش‌های نژاد بالب/سی به طور تجربی تومور پستان ایجاد شد و از بافت تومور </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:104%"><span new="" roman="" style="font-family:" times=""><span style="color:black">RNA</span></span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:104%"><span b="" nazanin="" style="font-family:"><span style="color:black"> استخراج شد. از </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:104%"><span new="" roman="" style="font-family:" times=""><span style="color:black">RNA</span></span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:104%"><span b="" nazanin="" style="font-family:"><span style="color:black">های استخراج شده </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:104%"><span new="" roman="" style="font-family:" times=""><span style="color:black">cDNA</span></span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:104%"><span b="" nazanin="" style="font-family:"><span style="color:black"> تولید شد و میزان بیان ژن‌های 10-</span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:104%"><span new="" roman="" style="font-family:" times=""><span style="color:black">IL</span></span></span></span><span style="font-size:9.0pt"><span style="line-height:104%"><span b="" nazanin="" style="font-family:"><span style="color:black"> و </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:104%"><span new="" roman="" style="font-family:" times=""><span style="color:black">VEGF</span></span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:104%"><span b="" nazanin="" style="font-family:"><span style="color:black"> با روش</span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:104%"><span new="" roman="" style="font-family:" times=""><span style="color:black">RT-PCR </span></span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:104%"><span b="" nazanin="" style="font-family:"><span style="color:black">&nbsp;ارزیابی شد. </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:104%"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:102%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:102%"><span b="" nazanin="" style="font-family:"><span style="color:black">یافته‌ها:</span></span></span></span></b><span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:102%"><span b="" nazanin="" style="font-family:"><span style="color:black"> آنالیز داده‌ها نشان داد که بیان ژن 10-</span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:102%"><span new="" roman="" style="font-family:" times=""><span style="color:black">IL</span></span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:102%"><span b="" nazanin="" style="font-family:"><span style="color:black"> و </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:102%"><span new="" roman="" style="font-family:" times=""><span style="color:black">VEGF</span></span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:102%"><span b="" nazanin="" style="font-family:"><span style="color:black"> بافت تومور نسبت به سلول‌های گروه شاهد بیشتر بود، اما این افزایش بیان از نظر آماری معنی‌دار نبود.</span></span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:102%"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span></span></span></span><br> <b><span dir="RTL" lang="AR-SA" style="font-size:9.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">نتیجه‌گیری:</span></span></span></span></b><span dir="RTL" lang="AR-SA" style="font-size:9.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black"> به طور کلی سطح بیان ژن‌های اینترلوکین-10 و فاکتور رشد</span></span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:9.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">اندوتلیال</span></span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:9.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">عروقی</span></span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:9.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">در مدل تجربی تومور افزایش داشت، اما تحقیقات وسیع‌تر و ارتباط با سایر فاکتورهای دخیل ضروری به نظر می‌رسد.</span></span></span></span> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:8.0pt"><span style="line-height:115%"><span ms="" style="font-family:" trebuchet="">Introduction:</span></span></span></b><span style="font-size:8.0pt"><span style="line-height:115%"><span ms="" style="font-family:" trebuchet=""> Breast cancer is one of the most common cancers and the principal cause of death in women. </span></span></span><span lang="EN" style="font-size:8.0pt"><span style="line-height:115%"><span ms="" style="font-family:" trebuchet="">One of the mechanisms of cancer cells for the lack of access to the immune system </span></span></span><span style="font-size:8.0pt"><span style="line-height:115%"><span ms="" style="font-family:" trebuchet="">is the production of compounds suppressing immune responses, such as interleukin-10. On the other hand, vascular endothelial growth factor, by binding to its receptors on the surface of endothelial cells, plays a significant role in vascular permeability and tumor vascularity. In this study, the expression of interleukin-10 and vascular endothelial growth factor in breast tumor tissue was investigated in an experimental tumor model.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:8.0pt"><span style="line-height:115%"><span ms="" style="font-family:" trebuchet="">Methods:</span></span></span></b><span style="font-size:8.0pt"><span style="line-height:115%"><span ms="" style="font-family:" trebuchet=""> First, mammary tumors were experimentally induced in Balb/C mice, and RNA was extracted from the tumor tissue. cDNA was synthesized from the extracted RNAs, and the expression level of 10-IL and VEGF genes was evaluated by RT-PCR.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:8.0pt"><span style="line-height:115%"><span ms="" style="font-family:" trebuchet="">Results: </span></span></span></b><span style="font-size:8.0pt"><span style="line-height:115%"><span ms="" style="font-family:" trebuchet="">The results of data analysis showed that the expression of IL-10 and VEGF genes in the tumor tissue was higher than in the cells of the control group, but this increase in expression was not statistically significant.</span></span></span></span></span></span></span></span><br> <b><span style="font-size:8.0pt"><span style="line-height:115%"><span ms="" style="font-family:" trebuchet="">Conclusions:</span></span></span></b><span style="font-size:8.0pt"><span style="line-height:115%"><span ms="" style="font-family:" trebuchet=""> In general, the expression level of Interleukin-10 and VEGF genes was increased in the experimental tumor model, but broader research and the correlation with other involved factors seem necessary.</span></span></span> فاکتور رشد اندوتلیال عروقی؛ اینترلوکین؛ تومور پستان Vascular endothelial growth factor, Interleukin-10, Breast tumor 205 210 http://jams.arakmu.ac.ir/browse.php?a_code=A-10-1940-3&slc_lang=fa&sid=1 Rana Noruozi Kuma Olya رعنا نوروزی کمارعلیا noorozi_a@yahoo.com 4600319475328460088486 4600319475328460088486 No Department of Biology, Payame Noor University, Tehran, Iran گروه زیست‌شناسی، دانشگاه پیام نور، تهران، ایران Sima Nasri سیما نصری s_nasri1@pnu.ac.ir 4600319475328460088487 4600319475328460088487 No Department of Biology, Payame Noor University, Tehran, Iran گروه زیست‌شناسی، دانشگاه پیام نور، تهران، ایران Samad Farashi-banab صمد فراشی بناب farashibs@yahoo.com 4600319475328460088488 4600319475328460088488 No School of Medicine, Tehran University of Medical Sciences, Tehran, Iran گروه زیست‌شناسی، دانشگاه پیام نور، تهران، ایران Fereshteh Dadfar فرشته دادفر fereshtehdadfar2003@yahoo.com 4600319475328460088489 4600319475328460088489 Yes Department of Biology, Payame Noor University, Tehran, Iran گروه زیست‌شناسی، دانشگاه پیام نور، تهران، ایران Naeimeh Dehghani نعیمه دهقانی naeimehdehghani@gmail.com 4600319475328460088490 4600319475328460088490 No Department of Biology, Payame Noor University, Tehran, Iran گروه زیست‌شناسی، دانشگاه پیام نور، تهران، ایران