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Introduction: Many research demonstrate the effect of margarine trans fatty acids on many physiological mechanisms in both human and animals. The purpose of this research was to evaluate the effects of margarine oil on reproduction in Wistar rat. Materials and Methods: ‏This is an experimental study in which 46 Wistar female and male rats were divided into 4 groups. Male and female rats in group 1 were fed by 3% Margarine oil and standard food, in group 2 only female rats and in group 3 only male rats had Margarine oil with the standard food. Male and female rats in group 4 (as control group) had only standard food. Administration was started one month pre mating and continued during mating, gestation and lactation periods. After delivery, the pups were assessed for overall number, sex ratio, body weight, and mortality. One way analysis of variance was used for data avalysis. Results: ‏Results showed that weight and female ratio of pups were significantly increased in group 2 as compared to group 1 and control. Also the comparison of pups’ mortality in the four groups showed that pups mortality was increased in group 2 as compared to the other groups. On the other hand there was no significant difference in number of pups in the four groups. Conclusion: The results showed that dietary Margarine oil administration to female rats can significantly increase weight, ratio of female pups and mortality of pups.

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Background and Aim: Recently, there is increasing concern about the declining of male reproductive health. Oxidative stress has been proposed as a possible mechanism contributed in reproductive system failure. Dorema aucheri that is being used in Persian folk medicine has been supposed to have male fertility-enhancing properties. Present study was done to evaluate the impacts of Dorema aucheri on oxidative status and reproductive parameters in male rats. Materials and Methods: In this experimental study, healthy adult male Wistar rats were treated with 100, 200 and 400 mg/kg of ethanolic D. aucheri leaves extract via gavage for 70 days. Blood samples were collected for analysis of testosterone, LH and FSH serum levels. Reproductive organs weight, density, motility and morphology of spermatozoa, seminiferous tubules diameter, germinal epithelium height and also testicular levels of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GPx) were evaluated. Findings: Significant (p<0.05) increases were seen in the testis and epididymis weights of male rats treated with 200 and 400 mg/kg D. aucheri extract. In rats treated with D. aucheri extract sperm density and percent of morphologically normal sperm were significantly (p<0.05) higher. No significant differences were seen in serum testosterone, FSH and LH levels between D. aucheri extract-treated groups and controls. D. aucheri significantly (p<0.05) reduced malondialdehyde (MDA) levels and also increased superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity in testicular tissue of rats. Conclusion: Present study indicates that D. aucheri leaves extract has beneficial effects on reproductive parameters in male rats which might be a consequence of its antioxidant properties.