Showing 8 results for Interleukin
Shahin Ramazi, Majid Motovalibashi, Morteza Hashemzade Chaleshtori, Hamid Reza Khazraei, Ali Fasihi, Elham Iziy,
Volume 17, Issue 12 (3-2015)
Abstract
Background: Allergic rhinitis, a chronic inflammatory disease of the respiratory tract, includes an IgE-mediated inflammatory response in the mucosal region of the aerial tubles. Since IL-8 plays a role in increasing the amount of immunoglobulin E and serves as an effective factor in the prevalence of this disease, so this study aims to investing at that whether IL-8 genotypes of 133 C/G polymorphism is associated with the amount of immunoglobulin E in serum in patients with allergic rhinitis.
Materials and Methods: In this analytical and descriptive study, by investigating 130 patients with AR and 62 healthy control volunteers in Chahar Mahalo Bakhtiari province, total level of IgE in serum was determined by ELISA method and then its association with 133 C/G polymorphism was investigated. T-test and chi-square statistical analyzes were performed using SPSS software for statistical analyses.
Results: the total serum IgE level of the patients with GC genotype of 133 C/G SNP were significantly higher regarding normal individuals (p<0.05).
Conclusion: The sigmificant relationship between 133 C/G genotype in IL-8 P gene promoter and increased serum IgE level confirms tht it plays a role in the prevalence of AR.
Rohullah Ranjbar, Abdolhamid Habibi, Farzaneh Abolfathi, Najeme Nagafian,
Volume 19, Issue 7 (10-2016)
Abstract
Background: Known inflammatory factors in type II diabetes and complications are delaying adjustment this factor, it is an effective approach in preventing complications.The aim of this study was to evaluate the effect of eight weeks aerobic interval training on interleukin-6 and interleukin-10 serum levels, in women with type II diabetes.
Materials and Methods: In this study, 18 patients with type II diabetes in Ahvaz city, were randomly divided into the two experimental group (n=10) and control group (n=8). The experimental group did the aerobic interval training and pedaling on the ergometer bicycle with 65 to 80 percent of their maximum leg power for three times a week during the eight weeks. The blood samples were prepared in two pre-test and post-test steps to measure the interleukin-6 and interleukin-10 serum levels through ELISA method.
Results: Statistical analysis showed that after eight weeks of aerobic interval training, interleukin-6 and interleukin-10 serum levels, related to the experimental group had no significant difference in comparison with control group (p>0.05).
Conclusion: The results indicate that doing the aerobic interval training for three times a week during eight weeks with 65 to 80 percent of maximum leg power, it has no effect on inflammatory and anti-inflammatory indices, in women with type II diabetes.
Bahman Hasanvand, Rahman Soori, Sadegh Abbasian, Mahsa Rastegar Moghaddam Mansoori,
Volume 20, Issue 1 (4-2017)
Abstract
Abstract
Background: In response to the exercise, variety of inflammatory and anti-inflammatory changes in cytokines were occurred into the tissue spaces and also to the systemic circulation. The purpose of the current study was to determine the effect of three-week intensive interval training on Lipocalin-2 and Interleukin1-β in healthy and adult rat hippocampus.
Materials and Methods: Twenty Wistar rats were divided into training and control groups. Training group performed 15 (bouts) × 4 (min) exercise training for 60 min with 85 to 90% of VO2max used for four sessions/week (for 3 weeks). Then, blood (for ELISA analyzing) and tissue sampling was performed from rat’s hippocampus and they were evaluated by using a Real-Time PCR method. Also, independent and paired t tests were used to define within and between group differences.
Results: Findings showed that gene translations of Lipocalin-2 and Interleukin1-β were significantly decreased in rat’s hippocampus (p=0.0001 and p=0.0001, respectively). Also, similar decreases were shown in serum levels of Lipocalin-2 and Interleukin1-β in rats (p=0.017 and p=0.003, respectively).
Conclusion: It seems that significant decrease of Lipocalin-2 subsequent of short-time intensive interval training is due to decreasing and significant changes in Interleukin1-β. Also, current training protocol on treadmill can significantly decrease levels of Lipocalin-2 and Interleukin1-β in rat serum and hippocampus.
Azam Zarneshan, Mohammadreza Zolfaghari, Mahdia Gholamnejad, Mehdi Yousefi,
Volume 20, Issue 8 (11-2017)
Abstract
Abstract
Background: IL-4 plays a key role in the pathogenesis of asthma and the increased serum level of that is positively correlated with asthma severity and obesity. IL-4 induces IgE secretion by B cells. Previous studies suggesting an anti-inflammatory effect of exercise training on asthmatic lungs. The aim of the present study was to investigate the effect of 12 weeks of aerobic exercise training on serum levels of IL-4 and IgE in overweight and obese asthmatic women.
Materials and Methods: 21 overweight and obese asthmatic women were selected and divided into two groups including experimental (n=12) and control (n=9) groups. The experimental group performed aerobic exercise training, three times a week, 60 minutes each session. Fasting blood samples were collected before and 2 days after completion of the course. The data were analyzed by using SPSS22 software
Results: A significant decrease in serum IL-4 (p = 0.015, t = - 2.68), BMI (p = 0.014, t = - 2.72) and weight (p = 0.001, t = -3.99) was shown following the 12 weeks of training in the experimental group compared to the control group. There were no significant correlations between the obesity changes and the Inflammatory Marker Changes (p>0.05).
Conclusion: The present study indicates that aerobic exercise training reduced the serum levels of IL-4 independent of obesity changes in overweight and obese asthmatic women.
Masoumeh Khodadoust, Masoumeh Habibian,
Volume 23, Issue 6 (11-2020)
Abstract
Background and Aim: Weight gain and vitamin D deficiency are associated with increased systemic inflammation and chronic diseases. The current study aimed to investigate the effects of regular Pilates training associated with Vitamin D intake on Tumor Necrosis Factor-α (TNF-α) and Interleukin-10 (IL-10) levels in overweight males.
Methods & Materials: This quasi-experimental study was conducted on 52 overweight men aged 45-55 years. Volunteers were firstly selected through the convenience sampling method. Then, they were divided into Pilates training, Pilates training + vitamin D supplementation, vitamin D supplementation (combined), and control groups (n=13/group). The provided Pilates training program was performed for 8 weeks (with the intensity of 50%-75% of reserve heart rate, 3 sessions/week). The supplement and combined groups also received 50000 units of oral vitamin D weekly for 8 weeks. The serum levels of variables were determined using the ELISA method. The obtained data were analyzed by Paired Samples t-test, one-way Analysis of Variance (ANOVA), and Kruskal–Wallis test at the significant level of <0.05.
Ethical Considerations: This study was approved by the Research Ethics Committee at Islamic Azad University, Babol Branch, Iran (Code: IR.IAU.BABOL.REC.1398.088). Also, this study was approved by the Iranian Clinical Trial Registration Center (Code: IRCT20190831044650N2)
Results: The collected results indicated that 8 weeks of Pilates training, vitamin D intake, and the combined intervention were associated with a significant increase in IL-10 (P<0.001) and a decrease in TNF-α (P<0.001). Additionally, the presented combination intervention was associated with further alternations in the research variables, compared to the other two interventions; however, in the exercise group, the decrease in TNF-α level was more than that in the vitamin D intake group (P=0.012).
Conclusion: It seems that in overweight combined with vitamin D deficiency stats, the anti-inflammatory effects of Pilates training, vitamin D supplementation interventions, and a combination of the two methods can be attributed in part to up-regulation IL-10 and TNF-α reduction; however, the effectiveness of the combined intervention on the development of inflammation was greater than that of the other two interventions. Furthermore, the effect of Pilates training on reducing TNF-α was stronger than that of vitamin D supplementation.
Fateme Halalkhor, Ameneh Poorrahim, Marefat Siahkouhian,
Volume 26, Issue 5 (12-2023)
Abstract
Background and purpose: Anti-cytokine considerations in covid-19 patients can play a significant role in preventing death, therefore, physical activity by reducing risk factors, as well as saffron with anti-inflammatory and antioxidant properties, are considered as anti-cytokine solutions. Therefore, the purpose of this research is to investigate the effect of saffron supplementation and endurance training on the concentration of interleukin-6, interleukin-10 and cysteine in outpatients with coronavirus.
Materials and methods: The statistical population of this research is outpatients with coronavirus, 36 of whom were randomly selected. Cytokines and cysteine were measured using a special kit and ELISA method. The research hypotheses were checked using SPSS software at a significance level of P<0.05 using ANOVA test with repeated measurements with between-group factor.
Results: The results showed that endurance activity and saffron supplementation caused a significant decrease in IL-6 levels and the ratio of interleukin 6 to interleukin 10 (P≥0.05). Also, endurance activity along with saffron supplementation caused a significant increase in serum L-cysteine concentration (P=0.035). The results showed that there is no interaction effect between the measurement stages and the group, as well as intra-group and inter-group differences in interleukin-10 variables (P=0.511).
Conclusion: The levels of IL-6 and IL-10 decrease due to saffron supplementation and endurance training, and the amount of cysteine increases, which helps the recovery of covid-19 patients.
Amir Najafi, Mohammad Amin Momeni-Moghaddam, Dr Davoud Salarbashi, Narges Amini Beidokhti, Marziye Rahmani, Milad Khorasani,
Volume 27, Issue 3 (7-2024)
Abstract
Introduction: Type 2 diabetes is a non-communicable disease that imposes a significant financial burden on the healthcare system each year. Numerous studies have demonstrated the involvement of inflammatory factors in the initiation and progression of this condition. The primary goal of this study is to compare the polymorphism of the interleukin 1 receptor antagonist gene among individuals with type 2 diabetes and those in the control group.
Methods: Following approval from the Ethics Committee of Gonabad University of Medical Sciences, blood samples were collected from 100 participants at Bohlool Hospital in Gonabad. These individuals were categorized into two groups: cases (individuals with type 2 diabetes) and controls (healthy individuals). DNA extraction was carried out using the salting out method. To examine the polymorphism, the specific segment was initially amplified through PCR with designated primers and then identified via gel electrophoresis. The data were analyzed using subjected to the Chi-square test at a significance level below 5%.
Results: Findings from the polymorphism analysis revealed a notable contrast in the genotype 2/1 (P = 0.001) and 2/2 (P = 0.004) within the case group when compared to the healthy participants. Specifically, individuals with genotype 2/1 exhibited a heightened risk of developing type 2 diabetes by up to 15 times.
Conclusions: Within the examined population, the polymorphism of the interleukin 1 receptor antagonist gene substantially influenced the predisposition to type 2 diabetes, amplifying the likelihood of developing this ailment. Individuals harboring allele 2 are at an increased susceptibility to type 2 diabetes.
Rana Noruozi Kuma Olya, Sima Nasri, Samad Farashi-Banab, Fereshteh Dadfar, Naeimeh Dehghani,
Volume 27, Issue 4 (10-2024)
Abstract
Introduction: Breast cancer is one of the most common cancers and the principal cause of death in women. One of the mechanisms of cancer cells for the lack of access to the immune system is the production of compounds suppressing immune responses, such as interleukin-10. On the other hand, vascular endothelial growth factor, by binding to its receptors on the surface of endothelial cells, plays a significant role in vascular permeability and tumor vascularity. In this study, the expression of interleukin-10 and vascular endothelial growth factor in breast tumor tissue was investigated in an experimental tumor model.
Methods: First, mammary tumors were experimentally induced in Balb/C mice, and RNA was extracted from the tumor tissue. cDNA was synthesized from the extracted RNAs, and the expression level of 10-IL and VEGF genes was evaluated by RT-PCR.
Results: The results of data analysis showed that the expression of IL-10 and VEGF genes in the tumor tissue was higher than in the cells of the control group, but this increase in expression was not statistically significant.
Conclusions: In general, the expression level of Interleukin-10 and VEGF genes was increased in the experimental tumor model, but broader research and the correlation with other involved factors seem necessary.
