Mohammad Ali Shariatzadeh, Ghasem Mosayebi, Naser Mahdavi Shahri , Ehsanollah Ghaznavirad, Ali Fani, Abdolrahman Dezfulian, Ali Chehreei ,
Volume 6, Issue 3 (10-2003)
Abstract
Introduction: Insulin-dependent diabetes mellitus (IDDM) or type 1 diabetes is an organ-specific autoimmune disease that caused by destruction of insulin-producing beta cells of the pancreas. Etiology of this disease is still unknown. It is seen that genetic and environmental factors play an important role for susceptibility to develop type 1 diabetes. The relationship between HLA associated factors and susceptibility to IDDM disease, was reported by several investigators. Also, some studies show that dermatoglyphics is associated with type 1 diabetes. However, it is maybe there is an association between HLA and dermatoglyphics inpatients'with type 1 diabetes and these characteristics could be applied for diagnosis of disease.
Materials and Methods: In this study, the prevalence of HLA (with using standard microlymphocytotoxicity method) and dermatoglyphics determined in 30 Iranian patients with IDDM and 30 normal healthy controls with similar ethnic background and the same geographical area.
Results: A significantly higher frequency of HLA-DQ, A2, DR3 and DQ2 were found in IDDM cases compared to the controls. The results obtain from dermatoglyphics showed that line ab was reduced in male and female type 1 diabetes. The reciever operating chractristics curve showed that the positive point for lines ab in right and left hands were 34.7 and 35.25, respectively.
Discussion: There is no association between HLA and dermatoglyphics.
With considering of genes encoding of HLA separated from genes determining dermatoglyphics, HLA typing and dermatoglyphics seem to be interesting tools for genetic studies related to type 1 diabetes. Further studies are recommended in order to provide more insight into the susceptibility to this disorder.
Dr Ghasem Mosayebi, Dr Ehsanola Ghaznavi Rad, Dr Ali Fani, Dr Seyedmohammad Moazenni,
Volume 8, Issue 3 (1-2006)
Abstract
Insulin dependent diabetes mellitus, human leukocyte antigens
Introduction: Insulin dependent diabetes mellitus (IDDM) or type 1 diabetes is created by autoimmune destruction of insulin-producing beta cells of the pancreas in genetically susceptible individuals. The relationship between human leukocyte antigens (HLA) associated factors and susceptibility to IDDM disease, was reported by several investigators. Association with different HLA types depends also on the studied populations. The aim of the present study was to determine HLA antigens which represent a high susceptibility to develop the IDDM disease in this area. Materials and Methods: In this study, the prevalence of HLA class-I and II antigens has been determined in 31 Arakian patients with IDDM and 57 normal healthy controls with similar ethnic background and from the same geographical area. The typing of HLA antigens was carried out using standard microlymphocytotoxicity method. Results: A significantly higher frequency of HLA-A2, A3, B21, DR3 and DQ2 were found in IDDM cases compared to the controls. In contrast, HLA-DR2, DR7 and B53 were represented at a somewhat higher frequency in controls compared to the IDDM patients. Conclusion: These results indicate that HLA-A2, A3, B21, DR3 and DQ2 antigens contribute to susceptibility to IDDM independently and HLA-DR2, DR7 and B53 antigens maybe associated with prevention of IDDM in Arakian patients.
Mana Shojapour, Ghasem Mosayebi, Fardin Faraji, Keyvan Faraji, Ali Ghazavi,
Volume 17, Issue 3 (6-2014)
Abstract
Background: Multiple sclerosis (MS) is an autoimmune disorder with unknown etiology. Genetic and environmental factors associated with MS susceptibility. Genetic studies show an important role for human leukocyte antigens (HLA) and susceptibility to autoimmune diseases such as MS. There is controversy between the association of HLA alleles with MS susceptibility in various studies. However, with consider the high incidence of MS in Iranian population and limit information about association of HLA and MS, we analyzed HLA alleles in MS patients.
Materials and Methods: In this case-control study, 60 MS patients and 40 normal individuals with the same ethic background and geographic area were analyzed for HLA-DRB and DQB alleles by single specific primer-polymerase chain reaction (SSP-PCR) method.
Results: HLA-DRB1*03 and DQB1*02 alleles frequencies in MS patients were greater than healthy controls. There was no significant difference in frequency of other HLA-DR alleles between the MS patients and normal individuals.
Conclusion: DRB1*03 and DQB1*02 alleles confer increased susceptibility to MS in this population. However, to determine the role of HLA in Iranian MS patients, more studies are needed.
