Showing 4 results for Formation
Alireza Shoae Hassani, Negar Ordouzadeh, Amir Ghaemi, Rashed Nazari, Kasra Hamdi, Davoud Hekmatpour,
Volume 11, Issue 2 (6-2008)
Abstract
Introduction: Extracts of leaves from Camellia sinensis L contains polyphenolic components with antimicrobial activity. In this investigation biofilm inhibitory effects of black and green tea extracts were defined for five members of enterobacteriacea family including: Enteropathogenic Escherichia coli, Shigella flexneri, Proteus mirabilis, Klebsiella pneumoniae and Salmonella enterica serovar Typhimurium. Because tea is the most widely drunk beverage in Iran, therefore investigation of its effects on enterobacterial biofilm formation and colonization is very important. Materials and Methods: In this experimental study after extraction of samples with Soxhlet extractor in water/ methanol solution, further extraction took place in Ethyl acetate phase. The extracts preserved in 4oC refrigerator after sterilization by 0.44 µ filters. Well diffusion (Kirby Bauer) and broth dilution methods were used for evaluation of minimum inhibitory concentration of biofilm formation in black and green tea extracts treated cultures. Evaluation of biofilm formation was assayed by observation of colony forming unit of cultured bacteria per milliliter by sampling from Erlenmeyer flask wall scratching onto Tripticase soy agar medium and comparing the results with controls. Analysis of data was done using analysis of variance. Results: Biofilm inhibitory effects of black tea were greater than green tea. The concentration of 4.5 mg/ml of black tea and 5mg/ml of green tea had bactericidal effects against examined bacteria. On Mueller Hinton agar, Proteus mirabilis was more sensitive to black tea EPEC was more sensitive to green tea and Klebsiella pneumoniae showed more resistance to both extracts. Conclusion: Due to the fact that gastrointestinal tract is directly affected with consumed beverage, the high concentration of tea entered in lumen can reduce the number of enterobacteriaceae and can reduce their carcinogenic amine products. Thus it plays an important role in inhibition of gastrointestinal lymphoma and colon carcinoma. Also application of tea polyphenols as a food preservative can be useful.
Other Information Journal,
Volume 12, Issue 3 (10-2009)
Abstract
Other Information About Journal
Behnaz Sadat Abedi, Zohreh Kiyani, Shahrbanoo Parchami, Morteza Hashemzade Chaloshtari, Abbas Doosti,
Volume 18, Issue 5 (8-2015)
Abstract
Background: Hypertrophic cardiomyopathy (HCM) is a various collection of heart diseases with autosomal dominant inheritance affecting 0.2% of the global population. HCM is also the most common cause of sudden cardiac death in individuals younger than 35 years old. Approximately, 40% of affected cases are associated with MYBPC3 gene. The aim of this study is to investigate the possible presence of mutation in 15 and 18 exons of MYBPC3 gene in patients with HCM in Chaharmahal Va Bakhtiyari province.
Materials and Methods: In this experimental study, 30 HCM patients were selected. DNA was extracted using standard phenol-chloroform method. Certain exons were amplified by PCR method. And then, SSCP and HA methods were run.
Results: Significant differences were observed between the positive control samples and other samples. However, there were no difference in studied exons or shift in the bands.
Conclusion: Mutations in the exons of MYBPC3 gene may cause the HCM disease, and change in other exons may be the causative agent in this geographical region and change in this studied exons may not have contributed to the HCM disease. However, it is necessary to study more patients for getting a better conclusion.
Sadaf Ataei Alamdari, Mohammad Arjomandzadegan, Mona Nassaj Manizani, Azam Ahmadi,
Volume 29, Issue 1 (3-2026)
Abstract
Background and Aim: Vancomycin-resistant enterococci (VRE) are among the common causes of nosocomial infections, and their ability to form biofilms may contribute to increased antibiotic resistance. This study aimed to evaluate the biofilm-forming ability of VRE isolates and their antibiotic resistance patterns.
Materials and Methods: A total of 70 stool samples were collected from cancer patients. Suspected Enterococcus isolates were identified through biochemical tests including catalase test (negative), growth in 6.5% NaCl, positive PYR test, and bile esculin hydrolysis. In total, 22 Enterococcus isolates were confirmed and preserved for further analysis. Resistance to vancomycin was determined using the Broth microdilution according to CLSI guidelines. Biofilm formation was evaluated using the microtiter plate assay. The presence of biofilm-associated genes gelE and cylA was detected by polymerase chain reaction (PCR).
Findings:
The results showed that 68.2% of the isolates were capable of forming biofilm. Based on optical density in the microtiter plate assay, 27.2% were strong biofilm producers, 13.6% moderate, and 27.2% weak. Additionally, 31.8% of isolates were non-biofilm producers. PCR results revealed that among the 22 isolates, gelE and cylA genes were present in 77.3% and 63.6% of cases, respectively.
Conclusion:
Biofilm formation plays a significant role in enhancing antibiotic resistance in VRE isolates. Identification of biofilm-associated factors can be beneficial in designing effective therapeutic strategies against these bacteria.
Ethical consideration
The ethics approval letter is available with approval ID IR.ARAKMU.REC.1403.047 and approval date 15/11/2023.
Funding:
This research was funded by Arak university of medical sciences, Iran.
