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Introduction: Radiotherapy after breast cancer surgery will increase local control of the disease and also increase overall survival. Radiation have some side effects on lung function. In different radiotherapy techniques, these side effects are different. Pulmonary function tests and oxygen saturation are methods for evaluation of these complications. In this study we decide to campare pulmonary complications in two radiotherapy methods. Materials and Methods: In this clinical trial study fifty one patients with breast cancer in stage II and III according to TNM staging system, which were under modified radical mastectomy in Imam Hosein hospital and refered for adjuvant radiotherapy, randomly divided in two groups. In one group patients were treated with three field technique and in others with four field technique. All patients received total dose of 48-50 Gy. For patients, pulmonary function test and pulse oxymetery were done once before initiation of radiotherapy and then one and three months after radiotherapy. Results: Measurement of FEV1 , FVC and show that no significant statistical difference was present between the two groups one month and three months after radiotherapy, also in each of the two groups the amount of FEV1 , FVC and one month after radiotherapy had no significant statistical difference in comparison to baseline tests but FEV1 and FVC after three months was decreased and had significant statistical difference respectively (p<0.001, p<0.006). SO2 had no significant defference between the two groups and also in each group after one and three month of radiotherapy. Conclusion: Locoregional radiotherapy of breast and lymph nodes areas causes a decrease in some parameter of pulmonary function tests but no difference was present between three field and four field techniques.

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Introduction: Extracts of leaves from Camellia sinensis L contains polyphenolic components with antimicrobial activity. In this investigation biofilm inhibitory effects of black and green tea extracts were defined for five members of enterobacteriacea family including: Enteropathogenic Escherichia coli, Shigella flexneri, Proteus mirabilis, Klebsiella pneumoniae and Salmonella enterica serovar Typhimurium. Because tea is the most widely drunk beverage in Iran, therefore investigation of its effects on enterobacterial biofilm formation and colonization is very important. Materials and Methods: In this experimental study after extraction of samples with Soxhlet extractor in water/ methanol solution, further extraction took place in Ethyl acetate phase. The extracts preserved in 4oC refrigerator after sterilization by 0.44 µ filters. Well diffusion (Kirby Bauer) and broth dilution methods were used for evaluation of minimum inhibitory concentration of biofilm formation in black and green tea extracts treated cultures. Evaluation of biofilm formation was assayed by observation of colony forming unit of cultured bacteria per milliliter by sampling from Erlenmeyer flask wall scratching onto Tripticase soy agar medium and comparing the results with controls. Analysis of data was done using analysis of variance. Results: Biofilm inhibitory effects of black tea were greater than green tea. The concentration of 4.5 mg/ml of black tea and 5mg/ml of green tea had bactericidal effects against examined bacteria. On Mueller Hinton agar, Proteus mirabilis was more sensitive to black tea EPEC was more sensitive to green tea and Klebsiella pneumoniae showed more resistance to both extracts. Conclusion: Due to the fact that gastrointestinal tract is directly affected with consumed beverage, the high concentration of tea entered in lumen can reduce the number of enterobacteriaceae and can reduce their carcinogenic amine products. Thus it plays an important role in inhibition of gastrointestinal lymphoma and colon carcinoma. Also application of tea polyphenols as a food preservative can be useful.

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Background: Molybdenum is an essential trace element for both animals and plants. Molybdenum (Mo), which functions as a cofactor for a limited number of enzymes including xanthine dehyrogenase, aldehyde oxidase, and sulfite oxidase in mammals, is believed to be an essential trace element in animal nutrition. The aim of this study is to evaluate the hepatoprotective potential of sodium molybdate against carbon tetrachloride (CCl4) induced liver damage. Materials and Methods: In an experimental study, adult male rats received daily oral administrations of different doses of sodium molybdate (0.05, 0.1, and 0.2 g/kg bw) along with intrapertioneal CCl4 (50% CCl4 in olive oil, 1 ml/kg bw) twice a week for 28 consecutive days. Results: Histopathological examinations in CCl4-treated rats showed extensive liver injuries characterized by extensive hepatocellular degeneration and necrosis, fat degeneration, and inflammatory cell infiltration while histopathological changes induced by CCl4 were significantly attenuated by sodium molybdate treatment. Conclusion: The results of this study suggest that sodium molybdate could protect liver against the CCl4-induced oxidative damage in rats, and this hepatoprotective effect might be contributed to the protection of liver by preventing the toxic chemical reactions which generate oxidative stress, lipid peroxidation, and molecular changes which ultimately lead to liver tissue necrosis.

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origanum vulgare l.spp viride was used in ancient medicine and it was medicated for digestive disease, diabetes, remedy of trauma. While antimicrobial, ant diabetic, anticancer and antioxidant effect of this plant was proved but there is done no study for its effect on reproductive system. Therefore the purpose of recent study is surveying eventual androgenic effect of this plant on hormonal level of pituitary - gonadal axis in mature male vistar rats. Materials and Methods: recent study was done on five groups of male rats of vistar race and every group includes nine rats. The control group received no drug. The sham group received physiological serum and experimental groups of A, B and C were received the ethanolic extract of origanum vulgare l. with concentration of 40, 20, 10 mg/kg body weight respectively with gavaj for 14 days. Then levels of FSH, LH and TSH hormones in blood sample was measured with RIA method and obtaining result was compared between control group and other groups. Data was analyzed with SPSS software and statistical method of ANOVA and Tukey test. In this research, significant level was p<0.05. Results: in control group, sham group and A, B and C groups, respectively mean and standard deviation from average of plasma concentration for LH hormone based on mlU /ml were: 0.18±0.006, 0.183±0.017, 0.187±0.026, 0.241±0.012 and 0.284±0.027 And for FSH Hormone were: 0.321±0.025, 0.342±0.071, 0.372±0.026, 0.383±0.031 and 0.372±0.026 And for TSH hormone were: 5.28±0.683, 6.07±0.5, 6.09±1.94, 6.66±1.48 and 8.1±1.66. Conclusion: ethanolic extract of origanum vulgare leaf in maximum dose have androgenic effects and it can affect the activity of different levels of hypothalamic – pituitary – gonadal axis and increase the secretion of testosterone and gonadotropic hormones.

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Background: Brucellosis is one of the most common bacterial zoonotic infections in the world. The incidence of this infection is quite high and is endemic in several countries. According to WHO report, the prevalence of zoonotic and human brucellosis is on the rise in the Mediterranean region, the Middle East, and west Asian countries. The aim of this study was to investigate the usage of silver nanoparticles in treatment of brucellosis. Materials and Methods: In this experimental study, the activity of silver nanoparticles against Brucella meltensis 16M was determined by agar well diffusion method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles were determined by macrodilution method. Also, the antibacterial effect of silver nanoparticles was studied in mouse model. Results: The results showed that silver nanoparticles in low concentrations can kill Brucella melitensis 16M in laboratory conditions. MIC and MBC of silver nanoparticles were 4 ppm and 6 ppm in macrodilution method, respectively. The anti-brucella effect of silver nanoparticles was also observed in mouse model. Conclusion: This study demonstrated that silver nanoparticles can be used against brucellosis.

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Background: Olive oil as a mjor source of fat in diet, besides having a high level of unsaturated fatty acid contains biological components, such as the antioxidant phenolic compounds that can prevent the destructive effect of free radicals and their resulting mutation on cellular structures. The main goal of this study was to investigate the antioxidant and anticancer effects of olive oil through Salmonella typhimurium and microsome. Materials and Methods: In this study, 16 Iranian olive oil samples and one Spanish sample were used. The determination test of anti-mutagenic potential was based on the method proposed by Ames et al. using mutant strain of Salmonella typhimurium TA100 and a well-known carcinogenic material (sodium azide) which by adding microsome of rat liver (S9), its anticancer effect was examined. Positive and negative controls which respectively contained sodium azide and distilled water were studied. Each test was simultaneously done three times and the percentage of inhibition was determined according to (1-T/M) × 100. Results: The inhibitory percentage in its highest level in darkness was equal to 63.64% based on the variety of olive oil and in light was equal to 60.70%. Conclusion: The findings of this study show that the antioxidant and anticancer properties of olive oil decrease with exposure to light

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Abstract

Background: Poor air quality has a lot of damage on the environment and humans. Awareness of the air quality situation reduces health effects of air pollution. This study was performed with the aim of the comparative investigation of Air Quality Health Index (AQHI) and its application in seven major cities of Iran in 2011.

Materials and Methods: This study was a descriptive–analytic one. First, the required data of four criteria pollutants were taken from Department of Environment in seven major cities of Iran. The data were validated by the World Health Organization criteria. The air quality health index was measured based on the instructions and classified into low, medium, high and very high degrees according to the air quality standard tables.

Results: The results demonstrate that according to air quality health index, the level of air pollution in seven major cities of Iran has been undesirable and air quality has exceeded the standard level in Ahvaz 85%, Arak73%, Tehran70%, Esfahan60%, Shiraz 47%, Tabriz 43% and, Mashhad 29% of days.

Conclusion: Due to poor air quality and health consequences resulting from it, importance of Air Quality Health Index is explored in planning to control and reduce air pollution and awareness of peoples from daily status of air quality and its health effects.

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Background and Aim: Herpes simplex virus type 1 (HSV-1) may lead to oral herpes, encephalitis and keratoconjunctivitis. Today, following the increasing of HSV-1 prevalence and drug resistance, there has been an interest in the use of natural substance. In this study, we assessed the effect of hexane and methanol extracts of Chelidonium majus L. against acyclovir-resistant HSV-1. Materials and Methods: The toxicity threshold of Chelidonium majus L. hexane and methanol extracts on HeLa cell was determined with trypan blue and MTT methods. Their direct antiviral effects were evaluated against HSV-1. Different concentrations of extracts in different times of virus replication have been evaluated. In each stage, the viral titers were tested by TCID50 assay. Findings: The methanol extract at the concentration of 200 µg/ml and hexane extract at the concentration of 600 µg/ml were determined as effective minimal cytotoxic concentration on HeLa cell line. These concentrations did not have significant virucidal effects on Herpes simplex virus. The maximum antiviral effects of methanol extract at the concentration of 200 µg/ml was exhibited 1 and 2 hours after virus adsorption and reduced virus titer 4 logTCID50 compared to the control. Hexane extract did not have antiviral effect. Conclusion: methanol extract of chelidonium majus L. compared to hexane extract showed significant antiviral effect on acyclovir-resistant HSV-1. Further research is required to identify specific bioactive compounds of this plant in order to be used in anti-herpes drugs.

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Background and Aim: There exists increased demineralization risk of enamel adjacent to fixed orthodontic attachments by cariogenic bacterias (e.g., Streptpcoccus mutans, Streptpcoccus sobrinus) and inadequate data about commonly used orthodontic cements. Thus, we evaluated the antibacterial effects of three common domestic and foreign orthodontic cements (Glass ionomer, Zinc phosphate, & polycarboxylate) on two cariogenic pathogens (S. mutans & S. sobrinus).
Methods & Materials: In this laboratory study, after culturing two standard strains of Streptococcus mutans and Subrinus bacteria and concentrating and diluting them, a direct contact test was used to evaluate the antibacterial properties of types of cement. The study groups (n=5) included the following: three types of glass ionomer resilience cement, Hoffman’s zinc phosphate and Hoffman’s polycarboxylate, and three glass ionomer cement, zinc phosphate, and carboxylate from Aria Dent factory in Iran. The acquired data were analyzed using SPSS v. 17.
Ethical Considerations: The present study was approved by Babol University of Medical Sciences (Code: MUBABOL.REC.1391.7).Results: In both types of culture media, containing Streptococcus mutans and Streptococcus subrinus bacteria, there was a significant difference between culture media containing 3 groups of cements respecting bacterial growth inhibition zone (P<0.001). In general, The mean diameter of the inhibition zone was greater in Iranian polycarboxylate, compared to its foreign counterpart (P<0.001).
Results: Regarding to zone of inhibition, there was statistically significant difference between three tested cements in both S.mutans and S.sobrinus culturing media (P<0.001). The mean diameter of inhibition zone was greater for Iranian polycarboxylate compared to its foreign counterpart (P<0.001).
Conclusion: Glass ionomer cement demonstrated better antibacterial effect compared to zinc phosphate and polycarboxylate cements. There was also greater antibacterial effect of three tested cements on S.sobrinus than S.mutans. Iranian polycarboxylate showed stronger inhibition on both mentioned bacterias compared to the foreign one.

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Background and Aim: The research results show tobacco use increases cardiovascular and lung disorders or the probability of periodontitis in humans, while no definitive relations were yet confirmed. This study evaluates the relationship between periodontitis and tobacco use by panoramic radiography in Bandar Abbas City.
Methods & Materials: A total of 198 panoramic radiographs were classified into periapical healthy (n=99) and patient (n=99) groups. Radiologists assessed radiographs for periodontitis via Total Teeth Numbers (TTN), the quality and length of Root Filling Treatment (RFT), the number (NoRFT), and inadequate root filling treatment (RFTin), age, gender, the cigarette and hookah use, and dental debris. Data were analyzed by Chi-Square, t-test, and logistic regression.
Ethical Considerations: Due to the lack of therapeutic intervention and the preservation of patients’ characteristics, ethical considerations are not included in this study (Code: IR.HUMS.REC.1399.384)
Results: Tobacco, hookah use, TTN, and RFTin were different (P<0.01) in groups, but cigarette smoking, debris, RFT, NoRFT, and age were not. Women in the periodontitis group showed high (P<0.01) in tobacco, hookah use, and RFTin, while no gender differences were observed in other parameters. The logistic regression results showed that based on the Odd Ratio NoRFT (39.7), RFTin (36.8), `hookah (8.1), tobacco (6.57), debris (3.62), smoking (3.59), RFT (1.4) and TTN (0.89) were the highest (P<0.01). At the same time, age and gender did not affect periapical tissues. 
Conclusion: Hokka, tobacco, and cigarette use negatively affected the periapical tissues, respectively. The decrease in TTN and increase in debris and RFT were probably the effects of tobacco smoking on the periapical tissues. Age and gender were not related to the periapical lesions.