Showing 4 results for Chorioallantoic Membran
Nayere Ataee, Jina Khayat Zade, Hassan Rakhshandeh, Saideh Zafar Balanezhad,
Volume 14, Issue 1 (3-2011)
Abstract
Background: Angiogenesis is one of the most important biological processes which is characterized by the formation of new blood vessels in many developmental and pathological stages. Therefore, angiogenesis blockage using anti-angiogenic drugs can be effective in treatment of such diseases as hemorrhages and cancers. Citrullus colocynthis (bitter melon) is a medicinal plant with cytotoxicity effects that its anti-angiogenic effects were investigated in this study. Materials and Methods: In an experimental study, at first, Citrullus colocynthis alcoholic extract was prepared. Then, 30 Highline fertilized eggs were randomly divided into control, sham-exposed, and treatment groups. On the seventh day of incubation, the sham-exposed group was treated with normal salin and the treatment group was treated with the plant extract. On the 10th day of incubation, CAMs were examined and photographed by research photostereomicroscope. The number and length of vessels around the treated region were measured and analyzed through SPSS and t-test (p<0.05). Results: According to data analysis, the number (31.40±5.87) and length (46.60±7.33 cm) of vessels in the control group did not reveal a significant difference in comparison to the number (27±5.16) and length (42.40±5.05 cm) of vessels in the sham-exposed group. However, a significant decrease was observed in the number (6.70±2.05) and length (14.79±3.34 cm) of vessels in the treatment group in comparison to the control group(p<0.05). Conclusion: The alcoholic extract of Citrullus colocynthis seems to have had a repressive effect on angiogenesis in chick chorioallantoic membrane. Therefore, it decreases the number and length of vessels around the treated area.
Fateme Niazi , Maryam Tehranipour, Khadije Shahrokhabadi,
Volume 19, Issue 7 (10-2016)
Abstract
Background: Angiogenesis is an important biological processes of new blood vessels in many pathological stages of development and embryo development occurs and a complex and dynamic phenomenon that is needed for development and other physiological processes. This study aimed to investigate the effect of alcoholic Ocimum basilicum leaf extract on angiogenesis chick chorioallantoic membrane is done.
Materials and Methods: In this experimental study 40 Ross fertilized eggs were randomly divided into four groups: control, sham-exposed and experimental groups were divided. The second day of incubation the eggs window was opened. Eighth day of the alcoholic extract of basil doses of 50 and 150 mg/kg on chick chorioallantoic membrane was injected. On day 12, embryos length and weight and chorioallantoic membrane (CAM) was photographed by photostereomicroscope Then the numbers and lengths of vessels in special area on CAM were measured with Image J. analyzed through by t-test and ANOVA (P<0.05).
Results: The data does not show significant difference between embryos length and weight in sham compare to all experimental groups. In the study vessels number just with 150 mg/kg observed significant.
Conclusion: Alcoholic extract of basil is an increase in the number of vessels and in this sense the healing and growth processes associated with them as well as effective.
Sara Ghandehari, Masoud Homayouni Tabrizi, Pouran Ardalan,
Volume 21, Issue 1 (4-2018)
Abstract
Abstract
Background: Angiogenesis occurs in physiologic (wound healing) and pathological conditions and plays an important role in tumor growth, progression and metastasis. Therefore, the inhibition of angiogenesis can be an important approach to treat cancer diseases. In recent years, the use of nanoparticles has been developed to control cancer cells and prevent tumor growth. Various studies have shown the role of nanoparticles in reducing angiogenesis and inhibition of cancer cells. So, in this study, the anti-angiogenic effect of silver nanoparticle synthesis by Rubina tinctorum L (Ru-AgNPs) on Chicken Chorioallantoic Membrane (CAM) was investigated.
Materials and Methods: In an experimental study, 60 eggs were randomly divided into 6 groups including control, laboratory control and 4 treatment groups (10 eggs/group). On the second day of incubation, a window was created on the eggs. On the 8th day, the chorioallantoic membrane of samples was treated with different concentrations of nanoparticles and on the 12th day, the image was taken from all samples. Finally, the number and length of vessels on the chorioallantoic membrane and following that weight and length of the embryo from crown to squattiest were measured. Quantitative data were analyzed using LSD-test.
Results: The results showed that the Ru-AgNPs significantly reduced the length and number of blood vessels in the treated groups compared to the control group. Also, the comparison of length and weight of embryos in treated samples with control group showed that the Ru-AgNPs had an inhibitory effect on the length of the embryos, but the weight did not differ significantly in the treatment with the control group.
Conclusion: Considering the inhibitory effect of the Ru-AgNPs on the angiogenesis of the chorioallantoic membrane, with further studies, this compound can be used to control and inhibit pathological angiogenesis in many diseases such as cancers.
Haleh Sadat Tavakkol Afshari, Masoud Homayouni Tabrizi, Touran Ardalan,
Volume 24, Issue 1 (3-2021)
Abstract
Background and Aim: Angiogenesis is the process of forming new blood vessels from the old ones; it plays an important role in physiological conditions, such as growth, wound healing, and reproduction. This process also plays a vital role in pathological phenomena and the occurrence of various diseases, especially tumor growth and metastasis. The present study evaluated the anti-angiogenic effects of nanoemulsion synthesized from Anethum graveolens essential oil using the CAM method.
Methods & Materials: To investigate the anti-angiogenic effects on the second day of incubation, a window was opened on the eggs. Subsequently, on the eighth day, a gelatin sponge with nanoemulsion with different doses (12.5, 25, and 50 μg/mL) was inserted on the chorioallantoic membrane. On the 12th day of incubation, a research stereomicroscope was used to evaluate the rate of angiogenesis and imaging of the treated area. Vascular factors were examined by Image J software.
Ethical Considerations: This study was approved by the Research Ethics Committee of Mashhad Branch, Islamic Azad University (Code: IR.IAU.MSHD.REC.1398.027).
Results: The obtained results concerning the effect of nanoemulsion on the rate of angiogenesis in the CAM test indicated a decrease in the length and number of vessels of the chorioallantoic membrane in the treated samples, compared to the controls. Moreover, there was a reduction in the height and weight of treated fetuses, compared to the untreated fetuses (P<0.05).
Conclusion: The anti-angiogenic activity of nanoemulsion synthesized from Anethum graveolens essential oil indicated the possible use of this substance for diseases, like cancer, in which the formation of blood vessels is inhibited. Therefore, Anethum graveolens nanoemulsion can be suggested as an agent for further cancer studies.
