Davood Bashash, Seyed H. Ghaffari, Maryam Kazerani, Kebria Hezaveh, Kamran Alimoghaddam, Ardeshir Ghavamzadeh,
Volume 15, Issue 9 (2-2013)
Abstract
Background: Since nearly 90% of patients with acute promyelocytic leukemia (APL) have high telomerase activity and significant shortened telomere length, these patients have, therefore, been suggested to be good candidates for the therapeutic intervention with telomerase inhibitors. This study was done to investigate the effects of BIBR1532, a non-nucleoside inhibitor of telomerase, on APL cells. Materials and Methods: In this experimental study, for investigating the effect of BIBR1532, NB4 leukemic cells were cultured in the presence of various concentrations of BIBR1532. Succeeding apoptosis assay, Caspase-3 activity assay, and quantitative real-time PCR were applied to examine the effect of this drug on apoptosis percenage, enzymatic activity of Caspase-3, and quantitative expression of genes mRNA involved in apoptosis. Results: The results showed that BIBR1532 induced apoptosis in NB4 cells in a dose-dependent maner. Moreover, real time PCR results showed that BIBR1532 led to a significant decrease in mRNA of Bcl-2 gene and signficant increases in transcription of Bax, PUMA, and Caspase-3. Conclusion: Since treatment with BIBR1532 could exert rapid apoptotic cell death in NB4 cells andactivate cellular apoptosis route, anti-telomerase-based therapy can regarded as a suitable strategy for APL treatment. Patients with progressive shortening of telomere length and high levels of telomerase activity are suitable candidates for treatment with telomerase inhibitors.
Mirza Hossein Norouzi Kamareh, Mohammad Reza Zolfaghari, Firouz Ghaderi Pakdel, Javad Tolouei Azar,
Volume 20, Issue 9 (12-2017)
Abstract
Abstract
Background: Telomerase activity was reduced by aging, leading to decrease in telomere length and cell death. Evidence suggests that physical activity as well as green tea has a positive effect on the prevention of cellular aging. The aim of this study was to evaluate the effect of 12 weeks aerobic training combined with green tea extract on cardiac telomerase enzyme in aged male rats.
Materials and Methods: In this study, 32 aged male rats were randomly divided into 4 groups: control, exercise, green tea and exercise + green tea. Exercise groups performed moderate intensity aerobic exercise for 12 weeks and 5 days per week; meantime, the supplementary groups consumed green tea extract. Sandwich ELISA method was used to measure the amount of telomerase.
Results: The results show that telomerase enzyme in exercise group was significantly higher than control group (p= 0.001), green tea group was significantly higher than control group (p= 0.016) and in exercise + green tea group significantly higher than control group (p= 0.001) was observed.
Conclusion: According to the results of this study, 12 weeks of moderate-intensity aerobic exercise with green tea extract can increased the amount of telomerase enzyme and Prevent telomere length shortening and cellular aging.
