Showing 5 results for Stroke
Fardin Faraji, Akram Ranjbar, Babak Eshrati, Afsoun Talaie, Nahid Shafie, Shadi Pirasteh,
Volume 11, Issue 3 (9-2008)
Abstract
Background: In the recent years, oxidative stress was attended as one of the causal factors of ischemic stroke. In terms of the role of genetic, geographic and ethnic factors in the prevalence of stroke, This study was designed to compare the oxidative stress indexes of stroke patients with normal healthy subjects in this geographic area. Methods and Materials: In this case-control study, 36 patients older than 50 years with ischemic stroke and 45 healthy subjects with same age and sex, were enrolled. Five milliliter blood were drawn from all subjects. Samples were centrifuged and plasma was separated. Total antioxidant capacity, lipid peroxidation and thiol levels were measured respettively by FRAP, TBA and HU methods. Then the result was analyzed using t-test. Results: Results showed total antioxidant capacity and thiol plasma levels were lower in stroke patients in compare to healthy subjects, but only the thiol group had significant difference(P=0/001). Although lipid peroxidation showed a slight but non-significant difference in stroke patients in compare to control group. Conclusion: These findings suggest oxidative stress in patients with acute ischemic stroke may be conseaqence of an imbalance in oxidant/antioxidant homeostasis. Therefore it may be useful to recommend antioxidant medications or diet for these patients.
Fardin Faraji, Afson Talaie Zanjani, Babak Eshrati, Shadi Pirasteh,
Volume 11, Issue 4 (12-2008)
Abstract
Background: Stroke is one of the important causes of death. However the prognostic factors are not clearly defined. There are many evidences which show the role of inflammatory factors during the progression of stroke but the relation of CRP and stroke is still vague. This study was designed to determine the relation between CPR Level and WBS with severity of stroke (according to CNSS score) and volume of infarct in stroke patients.
Methods and Materials : This is a cross sectional study performed during the winter and spring of 2007 on 49 patients with stroke hospitalized in Vali-e-Asr hospital of Arak.Clinical examination was done by using CNSS scoring. The CRP and WBC were measured in the first 72 hour of onset of the disease. The CT scanning together with infarct volume estimation was performed at the same time.
Results : There was no correlation between the volume of infarct with CRP serum level (r=-0.14, p=0.39 ) and WBC (r=-0.17, p=0.92 . Also there was no correlation between CNSS score with CRP level (r=-0.04, p=0.81) and WBC (r=-0.124, p=0.40) too, but there was a significant correlation between CNSS score and stroke volum in brain CT scan (r=-0.43, p=0.006 ).
Conclusion: According to data in this study there was no correlation between serum acute phase reactant, the volume and severity of infarction.
Majid Amiri Motlagh, Mohammad Ali Atlasi, Zeinab Vahidinia, Sayyed Alireza Talaei, Zeinab Rezazadeh Lavaf, Abolfazl Azami Tameh,
Volume 20, Issue 5 (8-2017)
Abstract
Abstract
Background: Glutamate is the most widespread excitatory neurotransmitter in the mammalian central nervous system (CNS) and plays major role in the pathogenesis of ischemia brain injury.Glutamate transporters have a major role in glutamate removal and maintain its concentration below excitotoxic levels. Although estrogen’s and progesterone’s neuroprotective effects were well-described, the exact molecular mechanism has yet to be determined. This study has investigated estrogen and progesterone effect on glutamate transporters expression in the ischemic penumbra/peri-infarct region in rat.
Materials and Methods: Adult male Wistar rats were subjected to transient middle cerebral artery occlusion (tMCAO) for 1 h. Estrogen and progesterone combination was immediately injected after tMCAO subcutaneously. Sensorimotor functional tests for evaluating behavioral deficits and TTC staining for measurement of infarct volume were performed 24 h after MCAO. Real-time PCR technique was used for gene expression analysis of glutamate transporters EAAT2 and EAAT3.
Results: The combination of estrogen and progesterone could significantly reduce lesion volume. Also, hormone therapy could improve ischemic neurological disorders. After hormone therapy, gene expression of glutamate transporters EAAT2 and EAAT3 did not show significant changes.
Conclusion: Combined estrogen–progesterone treatment significantly reduces neurological deficits and infarct volume; these effects are independent of the glutamate transporters signaling pathways.
Firoozeh Alavian, Sohrab Hajizadeh, Mohammad Javan, Roham Mazloom,
Volume 20, Issue 6 (9-2017)
Abstract
Abstract
Background: Recent studies indicate that hyperoxia has a significant therapeutic effect in the acute ischemic injury. The role of intracellular kinases, including ERK, has been posed in the phenomenon of ischemic tolerance. In the present study, the effect of intermittent normobaric hyperoxia on the activity of ERK in the stroke model was studied
Material and Methods: This is an experimental study. Animals include 4 groups (sham, hyperoxia–sham, stroke and hyperoxia–stroke); each group consisted of 6 male Wistar rats in the weight range of 250 to 350 grams. Hyperoxia groups were exposed to 95% inspired oxygen for 4 h/day and 6 consecutive days. Oxygen concentration in the control groups was 21% (normoxia, room air). After 24h, the stroke group animals were subjected to 60 min of right middle cerebral artery occlusion (MCAO). After 24h reperfusion, neurological deficit scores (NDS) and ERK activity were assessed.
Results: 5 hours after MCAO, stroke groups showed a significant increase in ERK activity in the cortex (p <0.01) and subcortex (p <0.001). At the same time, hyperoxia significantly increased the activity of ERK in cortex compared to the normoxia group (p<0.05). In subcortex, hyperoxia had no significant effect on ERK activity. Twenty-four hours after MCAO, stroke groups showed a significant reduction in ERK activity in the cortex (p <0.001) and subcortex (p <0.05). 24hr after MCAO, The activity of ERK in the hyperoxia groups; in both cortex and subcortex areas was significantly higher than that of the normoxia groups (p<0.05). Also, hyperoxia caused a significant decrease in NDS (p=0.0220).
Conclusion: Increased levels of ERK activity in the hyperoxia groups can be used to protect the nervous system, but the presence of other malicious factors may have been more effective in some cases, so that hyperoxia alone hasn’t been able to prevent stroke progression.
Firoozeh Alavian, Saeedeh Ghiasvand,
Volume 21, Issue 7 (2-2019)
Abstract
Background and Aim: Recent studies point to the protective effects of Stachys lavandulifolia against inflammation and oxidative stress. The purpose of this study was to investigate the neuroprotective effects of Stachys lavandulifolia on brain injury, blood-brain barrier permeability and edema volume in rat stroke model.
Materials and Methods: In this experimental study, 17 male Wistar rats groups (n=6) were used; including control groups, sham and groups receiving doses of 50, 75 and 100 mg/kg/day of extract; for 30 days and orally by gavage. Two hours after the last gavage, the stroke groups were subjected to the Middle Cerebral Artery Occlusion (MCAO) model. 24 hours later, the volume of stroke, the blood-brain barrier permeability (BBB) and the volume of edema were investigated in the experimental groups. Results were analyzed by one-way ANOVA, Bonffron post test through Graph Pad Prism software and designed through Excel.
Ethical Considerations: This study with research ethics code IR.UI.REC.1397.162 has been approved by research ethics committee at Isfahan University.
Findings: Pre-treatment with Hydroalcoholic Extract with doses of 50, 75 and 100 mg/kg/day reduced stroke size. The dose of 75mg/kg/day reduced the permeability of BBB and the edema volume compared with the stroke group.
Conclusion: The most effective dose of Stachys lavandulifolia is 75 mg/kg, which has a strong potential in neuroprotection and stroke prevention. This study could be useful in further investigating of neuroprotective effect of Stachys lavandulifolia. |
