Showing 5 results for Spermatozoa
Ali Khosrowbaki,
Volume 15, Issue 9 (2-2013)
Abstract
Background: There is growing evidence that damage to spermatozoa by reactive oxygen species (ROS) plays a key role in male infertility. This study was done to review the role of oxidative stress in male infertility. Materials and Methods: In this review article, PubMed, Scopus, and EBSCO-CINAHL databases were used for finding the relevant studies. Results: Under physiological conditions, a certain level of ROS is necessary for normal sperm function. However, an excessive level of ROS produced by leucocytes and immature sperms can cause damages to spermatozoa. Oxidative stress develops when there is an imbalance between ROS production and antioxidant defense system in male reproductive tract. High levels of ROS have been detected in the semen samples of 25-40% of infertile men. Oxidative stress can induce detrimental effects on standard seminal parameters and fertilizing capacity of spermatozoa. Conclusion: Oxidative stress can induce impaired sperm function that results in poor pregnancy rate in natural conditions and assisted reproduction.
Gholamreza Najafi, Rahim Hobe Naghi, Aref Hoshyari, Masoud Moghadaszadeh, Behzad Ghorbanzadeh,
Volume 15, Issue 10 (3-2013)
Abstract
Background: Atrazine is an herbicide used widely by farmers in controlling weeds. The aim of this investigation was to evaluate the effect of atrazine, as an herbicide, on sperm quality, sperm DNA damage, invitrofertilization (IVF), and embryonic development in mature male rats. Materials and Methods: In this experimental study, 42 mature male Wistar rats weighing 170±5g were divided into three groups, including one control and two treatment groups. The rats in the control group were administered corn oil (0.2 ml/day) and the rats in the test groups were orally gavaged with atrazine 150mg/kg (high dose) and 75mg/kg (low dose) body weight daily for a total of 45 days. Epidydimis tail was cut and placed in 1 ml of human tubular fluid (HTF) medium for 30 minutes in an atmosphere of 5% CO2 in air at 370C. The sperms were analyzed for sperm count, sperm viability, motility, DNA damage, immature sperm, and in vitro fertilization. Data were analyzed by One-Way ANOVA. Results: In this study, atrazine provoked a significant decrease (P<0.05) in sperm number, sperm viability, and sperm motility. The data suggest that the atrazine had a negative impact on sperm maturation and DNA integrity in a time-dependent manner, which consequently caused a significantly remarkable reduction in IVF ability (P<0.05). Conclusion: Atrazine is capable of inducing DNA damage and chromatin abnormalities of spermatozoa which can contribute to a low fertilization rate.
Seyed Mohammad Ali Shariatzadeh, Afsaneh Hajian Karahrodi,
Volume 17, Issue 12 (3-2015)
Abstract
Background: Bisphenol A (BPA) has estrogenic properties and generates reactive oxygen species (ROS). The aim of this study is to investigate the effect of Nigella sativa oil (NSO) on sperm parameters against toxicity induced with BPA.
Materials and Methods: In this experimental study, adult male mice with mean body weight 32±3 g were randomly divided into 4 groups (n=6) Control, BPA (200mg/kg/day), NSO (5ml/kg/day), and BPA+NSO. Oral treatment was performed till 34 days. After end of treatment, body and left testis weight were recorded and left caudal epididymis was also cut. Released spermatozoa were used to analyze sperm parameters such as motility, viability and abnormalities. Sperm chromatin quality was assessed. Data were analyzed with One-Way ANOVA.
Results: Body and testis weight showed no significant change in four groups (p<0.05). A significant decrease in the motility, viability and normal morphology of sperm (p<0.001) was found in BPA-treated mice compared to the control group. In BPA+ NSO group, NSO could significantly increas the mentioned parameters compared to the BPA group (p<0.05). BPA had no effect on the uncleus diameter of the spetmatogonia and sperm DNA integrity and histon-protamine replacement.
Conclusion: The results indicated that NSO could partially ameliorate Bisphenol A-induced toxicity on sperm parameters.
Ali Asghar Ghafarizadeh, Gholamhassan Vaezi, Seyed Mohammad Ali Shariatzadeh, Ali Akbar Malekirad,
Volume 20, Issue 6 (9-2017)
Abstract
Abstract
Background: In Asthenoteratozoospermic men, low motility, defected DNA and highly oxidative stress in sperm cause poor assisted reproductive techniques (ART) outcomes. The aim of this study was to determine the effect of Vitamin E (Vit E), as a potent antioxidant, on sperm motility, viability and DNA integrity at different times of in vitro incubation (after 2, 4 and 6-h) to improve asthenoteratozoospermic semen samples for ART.
Materials and Methods: Asthenoteratozoospermic semen samples of 50 volunteers were collected and examined. Each sample was divided into two groups of control and vitamin E (2mM) and kept in the 37 °C and 6 % CO2 for 2, 4 and 6 hours. After this incubation, sperm motility, viability and sperm DNA fragmentation (SCD) were evaluated in each group. Data were analyzed using repeated measurement of ANOVA and T-test. The means were considered significantly different at p<0.05.
Results:Significant decrease in total and progressive motility and viability as well as significant increase in sperm DNA damage (after 6h of incubation) were found in control group vs. the control group before incubation (p<0.05). The sperm motility and viability was significantly higher in vitamin E group compared to untreated control group (p<0.05). Our results also showed that DNA fragmentation significantly was lower after 6h of vitamin E treatment (p<0.05).
Conclusion: In vitro supplementation of vitamin E in asthenoteratozoospermia semen samples may protect spermatozoa from maltreatment effect of ROS during sperm sampling via keeping enzymatic and antioxidant process in optimum condition.
Mahsa Soltani, Elham Shojafar, Ali Asghar Ghafarizadeh, Azam Moslemi, Maryam Baazm,
Volume 28, Issue 6 (1-2026)
Abstract
Introduction: Sperm cryopreservation is a crucial method for preserving fertility in patients with asthenoteratozoospermia. However, this process can lead to a reduction in sperm parameters due to the production of free radicals and damage to the cell membrane. Various substances are added to the cryopreservation medium to prevent cellular damage. In this study, platelet-rich plasma (PRP), which contains growth factors and bioactive molecules, was used to improve sperm parameters after freezing.
Methods: Semen samples were collected from 20 men diagnosed with asthenoteratozoospermia. The samples were randomly divided into five groups: control (no PRP), PRP50, PRP100, PRP200, and PRP400. Homologous PRP was prepared and added to the respective groups. The sperm samples were cryopreserved in liquid nitrogen. Twenty days after freezing, samples were thawed and subjected to a comprehensive evaluation of viability, motility, morphology, malondialdehyde (MDA) levels, and DNA fragmentation using specific assay kits. The findings were evaluated using one-way analysis of variance followed by Tukey's post hoc test.
Results: The results of this study demonstrated that cryopreservation led to a significant decrease in all sperm parameters in the control group. The addition of PRP at concentrations of 50 and 400 among the concentrations used resulted in a significant increase in total motility, progressive and non-progressive motility, sperm viability, and a decrease in immotile sperm, DNA fragmentation, and MDA levels compared to the control group (p<0.05).
Conclusions: Cryopreservation and subsequent thawing can have detrimental effects on the biological properties of sperm samples. Therefore, the dose-dependent addition of platelet-rich plasma as a cryoprotectant may offer a promising approach to mitigate the negative impacts of freezing on samples from men with asthenoteratozoospermia.
