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Background: Clinical research suggest a relationship between epilepsy and hypogonadism. The aim of this study is to identify the impact of epilepsy induced by kindling with pentylenetetrazol (PTZ) on hormones and some reproductive parameters in male rats. Materials and Methods: Adult male rats (NMRI strain) were divided into control and kindling groups. Kindling was induced by 40 mg/kg PTZ with a 48 hour interval through intraperitoneal injection. Each animal received 13 doses. At the end of the thirteenth dose, animals were weighed and uthenaized with ether and blood samples, collected from their hearts, were used for testosterone, prolactin and FSH, LH assay. In order to examine motility and morphology of sperms, tissue samples from epidydimis were isolated and minced in DMEM/F12 culture medium and were incubated for 15 minutes. Then sperm morphology and motility were studied. Testis were also isolated and weighed. Results: Kindling with PTZ decreased serum levels of testosterone and LH, but it increased rolactin. However, there was no difference in serum level of FSH between the two groups. Sperm motility in kindling group decreased significantly. There were no significant differences in testis weight, but the amount of animal weight losses in the kindling group was more than that of the control group. Conclusion: By changing the serum level of sexual hormones and decreasing sperm motility, PTZ kindling induced epilepsy exerts diminishing negative effects on reproduction.

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Background: One of the side effects of chemotherapy agents is gonadal suppression leading to infertility. The present study examined the feasibility of using a gonadotropin releasing hormone agonist (GnRH-A) in the prevention of busulfan induced azoospermia in prepubertal mice.

Materials and Methods: In this case and control research, Leuprolide (3.8 mg/kg) was given to prepubertal male mice 2 weeks after or prior to busulfan (40 mg/kg) administration. The animals received one or two doses of leuprolide after busulfan administration with 4 weeks apart. Six weeks after the last leuprolide treatment, the sperm parameters were analyzed icluded sperm count and motility according to the World Health Organization criteria.

Results: At the time of scarifies, testicular weight were increased in animals receiving leuprolide. In animals receiving leuprolide pretreatment, the percentage of progressive and immotile sperm was significantly (p&le0.05) increased and decreased respectively. But the percentage of non progressive sperm did not change. The sperm motility in busulfan treated animals with post treatment didn't change. The sperm count also increased in pretreatment animals but it was not significant (p&ge0.05).

Conclusion: The present study demonstrates that leuprolide pretreatment resulted in improvement in sperm motility in prepubertal male mice.

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Abstract

Background: Diabetes mellitus is associated with reductions in fertility indices. Resistance training, on the other hand, through reducing the adverse effects of diabetes, exerts a positive impact on diabetic individuals. The aim of the present study was to examine the effects of ten weeks of resistance training on serum levels of reproductive hormones and sperm parameters in Wistar rats with diabetes mellitus type 2.

Materials and Methods: In this experimental study, 36 Wistar rats with mean weight of 200±50 were randomly assigned to healthy control, diabetic control and diabetic training groups. The diabetic resistance training group received ten weeks of resistance training (climbing up the ladder) following the induction of diabetes. Twenty-four hours after the last training session, left epididymis of the rats was examined for studying sperm parameters and blood serum samples were examined for evaluating reproductive hormones. Data were analyzed using one-way ANOVA and Turkey’s Post Hoc test at 0.05%.

Results: Ten weeks of resistance training induced significant increases in serum testosterone and FSH levels in the resistance training group in comparison to the diabetic group (p<0.007).Resistance training did not have any significant effects on serum LH levels in the resistance training group compared to the diabetic control group. In addition, sperm parameters (sperm count, survival rate and motility) presented significant improvements compared to the diabetic group(p<0.05).

Conclusion: Resistance training can improve sperm parameters, including sperm count, survival rate and motility, through increasing serum testosterone, LH and FSH levels (reproductive hormones) in rats with diabetes mellitus type 2.

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