Forough Khodadadnejad, Majid Akbari, Fateme Abdolalian, Mahboube Daneshvar, Behzad Ahmadi, Zahra Zahraei,
Volume 24, Issue 3 (8-2021)
Abstract
Background and Aim: The success of endodontic treatment depends on the complete elimination of pathogenic bacteria inside the root canal. Enterococcus faecalis, a gram-positive, pathogenic, and anaerobic cocci, are the primary etiology of root canal infection in treated and untreated teeth and are highly associated with endodontic treatment failure. This study aimed to compare the antimicrobial activity of Sodium hypochlorite 2.5%, microemulsion of Myrtus 10%, and microemulsion of Thyme 0.6% on the E. faecalis after root canal filling.
Methods & Materials: In this experimental study, 25 extracted single canal human anterior teeth were collected, and their crowns were cut. Then after washing and cleaning, and filling, the sterilization was done using an autoclave. Then, except 5 teeth (negative control group), the teeth were smeared with E. faecalis and then divided into 5 groups randomly represented the irrigation by Sodium hypochlorite 2.5%, microemulsion of Myrtus 10%, and microemulsion of Thyme 0.6%, with a positive control group and negative control group. The teeth were filled with gutta-percha and then incubated in an incubator for 90 days at a temperature of 37°C. Finally, the comparison of CFU in the groups due to abnormal data distribution was performed using the Kruskal-Wallis test.
Ethical Considerations: The Ethics Committee of Arak University of Medical Sciences approved this study (Code: IR.ARAKMU.REC.1398.297).
Results: According to the results of this study, the correlation between the groups of cultured teeth was not significant(P>0.05). The specimens washed with microemulsion of the Thyme 0.6% had the highest positive number of cultures; the specimens washed by Sodium hypochlorite 2.5% had the lowest number of cultures.
Conclusion: This study showed that no statistically significant difference exists in their antimicrobial activity after root canal therapy after the use of different wash solutions.
Dr. Mojtaba Bayani, Dr. Shirin Shafiei Lialestani, Dr. Amir Almasi-Hashiani, Dr. Seyed Hamed Mirhoseini,
Volume 25, Issue 5 (12-2022)
Abstract
Introduction: Dental unit water lines (DUWL) are a potential place for the accumulation of microorganisms and the formation of microbial biofilm, which exposes people to infection risk. This study aimed to investigation of the effectiveness of four commonly used substances and selection of most effective disinfectant in DUWLs disinfection.
Methods: This systematic review study was conducted based on PRISMA templates and the PubMed, Scopus and Web of Science databases with a specific search strategy were examined. In this systematic review study, the effectiveness of 4 disinfectants including: hydrogen peroxide, chlorhexidine, chlorine-dioxide, sodium hypochlorite on microbial biofilms in the DUWL was investigated. The study was conducted on July 10, 2021, and finally the data of all studies related to the subject of this systematic review were extracted. This study was approved by the Research Ethics Committee at Arak University of Medical Sciences (Code: IR.ARAKMU.REC.1399.347).
Results: All four disinfectants in sufficient concentration and time can be useful and effective. If the biofilm in DUWL is old and stabilized, it will affect the effectiveness of these materials and it will take longer to remove.
Conclusions: The use of materials in combination can cover all the microbial spectrum present in the biofilm of this area, and even fixed biofilms can be removed with extended and continuous use.
