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Background: Nutrition assessment is a routine method for determining the nutritional needs of individuals, especially for vulnerable groups (women, infants, and children). One of the important criteria for assessment of nutritional status is determining the concentration of a nutrient after its intake. Materials and Methods: In this cross-sectional study, 80 lactating mothers were selected from private and public hospitals in Tabriz. Data gathered through the questionnaire included general information concerning lactating mothers and also their nutritional diet. Since vitamin A and beta-carotene were measured through Karr-Price method, vitamin A and beta-carotene concentrations were measured at 620 nm wavelength. Results: There was a significant positive correlation between retinol intake and colostrum retinol of lactating mothers (p≤0.01) (r = 0.28). Also, retinol intake showed a positive and significant correlation with total vitamin A (retinol equivalent) in colostrum (p≤0.002) (r= 0.33). Conclusion: Daily retinol and beta-carotene intake affect retinol of colostrum which its proper concentration in mother’s milk can play an important role in better function of this vitamin for both mother and child.