Showing 5 results for Roc Curve
Mahmood Amini, Seid Alireza Hosseini, Yahya Jand, Tooraj Zandbaf, Babak Eshrati, Shabanali Alizadeh, Ghasem Mosayebi, Ali Ghazavi, Ali Cyrus,
Volume 13, Issue 4 (1-2011)
Abstract
Background: Nowadays, the use of inflammatory biomarkers in the diagnosis of appendicitis is on the rise. On the other hand, the role of oxygen free radicals in various inflammatory states has been verified. Noticing the high prevalence of negative appendectomy (9.3-22.2%), the aim of this study was to investigate the level of blood total antioxidant capacity (TAC) as a biomarker for early diagnosis of acute appendicitis. Materials and Methods: In this prospective analytical trial, over a one-year period, 407 patients, with preoperative diagnosis of acute appendicitis who had undergone operation, were studied. After measuring the TAC level, data were analyzed through the analytic ROC curve and parametric mean comparison tests. Results: Of all the patients, 298 were identified with appendicitis. TAC levels in adult female and male groups were respectively 663.9±164.75 and 752.58±167.37 µm/L which revealed a significant difference (p<0.0001). Also, in children, the mean of TAC level in perforated appendicitis sub-group was significantly greater than those in gangrenous and suppurative groups. Conclusion: TAC level can be used as a predictive factor for the diagnosis of perforated appendicitis in children however, it cannot be used as a biomarker for the diagnosis of appendicitis. Because of the significant difference existing between the level of TAC in male and female patients, further studies are suggested to investigate the level of inflammatory biomarkers with respect to menstrual cycle in women.
Mahmood Amini, Tooraj Zandbaf, Shabanali Alizadeh, Yahya Jand, Seid Alireza Hosseini, Babak Eshrati, Ali Cyrus, Hamideh Amini,
Volume 14, Issue 1 (3-2011)
Abstract
Background: Diagnosis of acute appendicitis is one of the challenges that surgeons face in the emergency ward. Although Alvarado score has been recommended in the diagnosis of acute appendicitis, this criterion has some limitations in terms of age, sex, and race. The aim of this study was to evaluate the effectiveness of Alvarado score in the diagnosis of acute appendicitis based on the age and gender. Materials and Methods: In this cross-sectional study, 407 patients who underwent operation with the preoperative diagnosis of acute appendicitis from June 2008 to August 2009 were included in the study. The patients’ data were recorded by the investigators. The data were compared by parametric tests. The sensitivity and specificity were determined by the optimal cut-off point on ROC curve using MedCalc software version 10.2.0.0. Results: The optimal cut-off point for women was lower than men (6 and 7, respectively). The rate of negative appendectomy in the group with Alvarado scores 9 and 10 was 11.8%, while the negative appendectomy rate in the other two groups with Alvarado scores 7 and 8 and 5 and 6 were 29.9% and 52.8%, respectively. Conclusion: The diagnostic value of Alvarado score of 8 and less in physical examination is lower than the abdominal tenderness exam alone. Also, the cut-off point for the diagnosis of acute appendicitis in women should be considered lower than that in men. On the other hand, the cut-off point in adolescents and children is higher than adults.
Hamideh Mohammadnia Kojidi, Mohammad Rafeie, Mohammad Ali Daneshmand, Jalal Rezaei,
Volume 21, Issue 2 (5-2018)
Abstract
Abstract
Background: Early diagnosis of prenatal illnesses and timely treatment of congenital anomalies has been the mainstay of the health system. In this study, our aim is to provide Roc curve multivariate modeling in detection of fetal abnormalities using associated markers in screening Down syndrome in the first and second periods of pregnancy.
Materials and Methods: This is a descriptive-analytical study that uses information from two sets of data. In the first set, 152 individuals, who had the results of the first- trimester and second screening tests at risk and in the second group, 75 individuals with normal results. The studied variables included the serum markers in the first- trimester and the second- trimester screening, auxiliary variables (includes demographic information). Statistical analysis was performed by using ROC regression, incremental value analysis and Stata 12 software.
Results: In evaluating the value of each diagnostic test in the presence of auxiliary variables using logistic regression and rock curves, the results generally showed that in screening the first- trimester of PAPP-A and in the screening the second-trimester,Inhibin-A can be used alone as a diagnostic test.
Conclusion: Best diagnostic test in the first- trimester, respectively, PPAP-A, NT, FREE B-HCG and in the second- trimester of screening, respectively, Inhibin-A, HGG, UE3 and AFP were based on the area under the ROC curve. In addition, the most significant effect of the predictor variable on the outcome of the diagnostic test was family history.
Mojtaba Asad Samani, Maryam Peymani,
Volume 24, Issue 2 (5-2021)
Abstract
Background and Aim: The protein encoded by the SGO1 gene is a member of the shugoshin family of proteins and protects the centromere during mitosis. lncRNAs are non-coding RNA with 200 nucleotides lengths, i.e., involved in regulating gene expression. The current study aimed to evaluate the expression of SGO1 and SGO1-AS1 in different stages of disease progression; we also compared their expression pattern in tumor tissues with healthy tissues in colorectal cancer patients.
Methods & Materials: In total, 40 tissue samples of patients with colorectal cancer were reported according to the examination and criteria with the approval of a pathologist. Besides, 40 normal tissues were sampled from a completely healthy part of the intestine of the same patients. After RNA extraction and cDNA synthesis, the Real-time RT-PCR technique was used to evaluate the expression of the desired genes in the study groups. ROC curve analysis was also used to determine the ability of each selected gene to diagnose the disease.
Ethical Considerations: This study was approved by the Ethics Committee of Shahrekord Azad University (Code: IR.IAU.SHKREC.1398.020).
Results The obtained data suggested that SGO1 significantly decreased in the colorectal cancer tumor samples (P<0.001) and SGO1-AS1 LncRNA significantly increased expression, compared to adjacent healthy tissues. Additionally, in the age group of below 60 years, compared to the age group of over 60 years, SGO1 expression increased and SGO1-AS1 expression decreased. Based on the AUC obtained from the ROC diagram, it was found that the SGO1 gene with AUC=0.8041 and SGO1-AS1 with AUC=0.6364 could significantly distinguish a healthy population from patients with colorectal cancer.
Conclusion: According to the collected results, SGO1 -AS1 and SGO1 were significantly reduced and increased in tumor tissue, respectively; however, only the SGO1 gene was introduced as a good marker for diagnosing colorectal cancer.
Reza Hashemi, Maryam Peymani, Kamran Ghaedi, Hana Saffar,
Volume 25, Issue 1 (3-2022)
Abstract
Background and Aim PBK is a mitogen-activated protein kinase (MAPKK) among MEK1/2 and MEK7 and can phosphorylate P38, JNK, and ERK in many cellular functions. The E2F transcription factor family also belongs to a class of cellular regulators acting as oncogenes and tumor suppressors. This study aims to investigate the expression of PBK and E2F7 in the early stages of colorectal cancer (CRC) compared to advanced stages based on the experimental and TCGA (The Cancer Genome Atlas) database.
Methods & Materials A total of 32 tissue samples of patients with CRC with the approval of a pathobiologist were collected according to the examination and criteria reported from different stages. After RNA extraction and cDNA synthesis, the RT-qPCR technique was used to evaluate the expression of the desired genes in the study groups. A receiver operating characteristic (ROC) curve analysis was also used to determine the ability of each of the selected genes to differentiate the two populations: stage I+II and stage III+IV.
Ethical Considerations In all stages of this research, codes of ethics of research and publication were observed.
Results In this study, it was shown that the expression of PBK and E2F7 significantly increased in stage I+II samples compared to stage III+IV. These data were confirmed by laboratory results and information extracted from the TCGA database. Also, based on the area under curve obtained from the ROC curves, these two genes are significantly distinguishable between stage I+II and III+IV populations in CRC.
Conclusion According to the results of this study, PBK and E2F7 genes are good markers in the diagnosis of CRC.
