Showing 7 results for Pcr-Rflp
Monir Doudi, Gilda Eslami, Mahbobe Setorki, Seyed Hossin Hegazi,
Volume 14, Issue 4 (9-2011)
Abstract
Background: Leishmania major and leishmania tropica are the main causes of cutaneous leishmaniasis in Iran, especially in Isfahan and Bam regions. In this study, noticing the effect of diversity of this parasite strains on designing disease control strategies, human isolates were examined through PCR-RFLP to determine the type of strains. Materials and Methods: In this experimental study, 340 samples obtained from CL patients due to Leishmania were cultured and prepared for microscopic study and examined through PCR-RFLP. The products of some of these samples were sequenced and analyzed. ITS1 region of genomic DNA was extracted and amplified with LITSr and L5.8s primers. Data on sequencing the samples were related to ITS1 region that in extracted DNAs with LITSr and L5.8s primers appeared with four kinds of genotype patterns, two for L.major and two for L.tropica in Isfahan and Bam regions. Results: Genotypic groups, LmA and LmB, were detected from L.major isolates while LtA and LtB genotypic groups were indicated for L.tropica in these two regions. The most prevalent genotypes related to isolates of Isfahan were LmA geneotype, whereas LtA geneotype was mostly reported in isolates of Bam. Conclusion: Leishmania major and leishmania tropica, the causative agents of zoonotic cutaneous leishmaniasis (ZCL) in Isfahan and anthroponotic cutaneous leishmaniasis (ACL) in Bam, respectively, are genetically polymorphic species. There exists a relationship between genetic heterogeneousness and clinical manifestation and geographical regions of this disease in human
Abedin Saghafipour, Yavar Rassi, Mohammad Reza Abai, Mohammad Ali Oshaghi, Mohammad Reza Yaghoobi Arshadi, Mehdi Mohebali, Homa Hajaran, Reza Mostafavi,
Volume 15, Issue 6 (11-2012)
Abstract
Background: Cutaneous leishmaniasis is a health problem in many areas of Iran. Cutaneous leishmaniasisis reported mostly in the central county of Qom province, including Ghanavat and Qomrood villages. This study was done to identify parasite species in human and rodents in order to illustrate the epidemiologic picture of the disease and provide an appropriate control program in 2010 Materials and Methods: This cross-sectional study was done on microscopic smears of 45 human samples and rodents samples hunted around Ghanavat and Qomrood villages in the central county of Qom province in 2010.In total,15 human samples and one hunted rodent sample were positive. In this study,the DNA of the parasites were extracted from the slides and analyzed by Leishmania specific premiers using ITS1 PCR-amplification (Internal Transcribed Spacer1). PCR (PolymeraseChain Reaction) products were digested by Haelll enzyme. Results: Overall, 15 human samples and one rodent sample from Merioneslibycus species were evaluated by PCR-RFLP (Restriction Fragment Length Polymorphism). After electrophoresis, it was demonstrated that the parasite was Leishmaniamajor in both human and rodent species. Conclusion: PCR-RFLP technique is an effective method to determine Leishmania parasite species in Geimsa stained slides from human and rodent reservoirs. One of the advantages of this technique is that it is possible to recognize the pathogen species of Leishmania parasite without gene sequencing. Besides, PCR-RFLP technique is a method of quite high sensitivity and specificity which can identify parasite species in addition to the diagnosis of leishmaniasis within 24 hours.
Mohsen Soosanabadi Farahani, Kourosh Kamali, Masoud Karimlou, Mehdi Banan, Hamid Reza Khorram Khorshid,
Volume 16, Issue 6 (9-2013)
Abstract
Background: There is abundant evidence indicating that inflammatory mechanisms within the central nervous system contribute to cognitive impairment via cytokine-mediated interactions between neurons and glial cells. BAT1, a member of the DEAD-box family of RNA helicases, appears to regulate the production of inflammatory cytokines associated with AD pathology. In the current study BAT1 -22 promoter polymorphism was analyzed in AD and control subjects.
Materials and Methods: In this case-control study, genomic DNA from peripheral blood samples of 153 Alzheimer’s patients and 153 healthy controls was extracted using salting-out method. DNA analysis was performed by PCR-RFLP method and p<0.05 was considered statistically significant.
Results: After genotyping and statistical analysis the results failed to show any association between BAT1 -22 promoter polymorphism and sporadic Alzheimer’s disease.
Conclusion: BAT1 -22 is not associated with Alzheimer’s disease in Iranian population and so has no effect on predisposition to sporadic Alzheimer’s disease.
Shahin Ramazi, Majid Motovalibashi, Morteza Hashemzade Chaleshtori, Hamidreza Khazraei,
Volume 17, Issue 2 (5-2014)
Abstract
Background: Allergy is regarded as a multifactorial condition that its onset and severity are influenced by both genetic and environmental factors. Hence, identification of genetic factors involved in allergic rhinitis development and its related phenotypes is a major task in understanding the genetic background of allergic rhinitis. This study was designed to examine the association between IL-18 -607 A/C promoter polymorphism on chromosome 11q22 and allergic rhinitis.
Materials and Methods: In this analytic study, genomic DNA was obtained from the blood samples of 293 patients with allergic rhinitis and 218 healthy controls by standard phenol chloroform method. The IL-18/-607 A/C polymorphism was analyzed by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis. To analyze the association between genotypes and alleles and the disease in the case group compared with the control group, X2 test was used.
Results: The frequency of the AC genotype of the IL-18/-607 A/C gene polymorphism was significantly greater in allergic rhinitis patients than in controls (p<0.05). By comparing the frequency of AA genotype with other genotypes, OR was calculated as 2.03.
Conclusion: The results suggest that IL-18/-607 A/C polymorphism gene may be one of the factors participating in the pathogenesis of AR or its intermediary phenotypes.
Sonya Zamani, Farhad Mashayekhi, Zivar Salehi, Nasim Abbasi,
Volume 18, Issue 11 (2-2016)
Abstract
Background: Diabetic retinopathy (DR) is the complication of diabetes mellitus (DM) and causes blindness among adults. Chronic extra cellular hyperglycemia in diabetes stimulates reaction oxygen species ROS production and increases oxidative stress. GPX-1 that was coded by GPX-1 gene is a key enzyme in protecting vessels against oxidative stress. The aim of this study was to evaluate the association of GPX-1 gene Pro 198 Leu polymorphism in patients with diabetic retinopathy.
Materials and Methods: In this case-control study, 160 blood samples of participants including 80 patients with diabetic retinopathy and 80 healthy individual were tested. Genotyping of GPX-1 gene was determined by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) by ApaI enzyme. Data analysis was performed using MedCalc (12.1) program.
Results: The genotype frequencies of the GPX-1 in DR patients for Leu/Leu, Leu/Pro, Pro/Pro were 10%, 62.5% and 27.5%, respectively, while for the control groups were 10%, 70% and 20%, respectively.In ohter words, Ile/Pro heterozygote was the most frequent genotype in patients and controls. According to the results of this study, there was not significant difference between patients with diabetic retinopathy and controls(p=0.52).
Conclusion: It is concluded that GPX-1 gene Pro 198 Leu polymorphism is not associated with DR. Further research is required to clarify the role of GPX-1 gene in DR in Rasht population along higher sample size.
Ahmad Hamta, Milad Pezeshki, Jamshid Ansari,
Volume 20, Issue 12 (3-2018)
Abstract
Abstract
Background: Biological and epidemiological data suggest that damage induced by endogenous and exogenous factors affects the integrity and stability of DNA and associated with susceptibility to breast cancer. The XRCC3 protein participates in DNA double-strand breaks and recombination repair. The aim of the present study was to evaluate associations between the risk of breast cancer and Thr241Met polymorphism in the XRCC3 gene.
Materials and Methods: In this study, the effects of Thr241Met polymorphism of the XRCC3 gene and the risk of breast cancer in a population-based case-control study inclusive 80 patients and 80 healthy individuals of women in Markazi province were evaluated. Genomic DNA was extracted from blood samples using the kit procedure. The genotypes of samples were determined by PCR-RFLP technique. Statistical analysis was done using SPSS software (estimation of χ2 and p-value) and the final results were determined.
Results: Statistically significant difference was observed between the two groups of patients and controls for three genotypes of the site rs861539 (p= 0.000). Genotype CT (p= 0.000, OR=2.352, CI= 95%; 2.431 - 39.948) and TT (p = 0.003, OR= 2.352, CI=95%; 0.611 - 9.049) significant associations were showed with risk of breast cancer. Instead, the genotype CC (p= 0.000) showed a protective role against susceptibility to breast cancer.
Conclusion: This study identified that there is significant association between Thr241Met polymorphisms of the XRCC3 and the risk of susceptibility to breast cancer, which is in accordance to some of researchers' studies.
Seyedeh Zahra Shifteh, Doctor Ahmad Hamta,
Volume 26, Issue 1 (4-2023)
Abstract
Introduction: Breast cancer is a highly heterogeneous disease. The antigen molecule of four cytotoxic T-lymphocytes is involved in inhibition of T cell response and immune response regulation. Single nucleotide polymorphisms in the CTLA4 gene can affect the expression of the aforementioned molecule. The aim of this study was to investigate the polymorphisms of rs4553808 and rs733618 of CTLA4 gene with the risk of breast cancer.
Methods: In this study to investigation polymorphisms, the DNA of 80 patients with breast cancer and 80 healthy individuals in central province of ARAK were extracted from peripheral blood. Then, PCR-RFLP technique was used. The results were analyzed using SPSS software and SNP Analyzer. This study was approved by the Ethics Committee of the Arak University (Code: Ir.arakmu.rec.1396.25).
Results: Statistical analysis rs4553808 polymorphism showed no significant increase in the risk of patients with GG genotype compared with the control group (OR = 2/013, CI = 95% 1/721-2/353). Also, heterozygotes AG genotype analysis did not show any relationship between the genetic diversity and breast cancer (OR = 1/204, CI = 95% 0/604-2/402). The combination of AG + GG genotypes did not show any significant correlations (OR = 1/130, CI = 95% 0/569-2/242). Statistical analysis for rs733618 polymorphism showed increase in the risk of breast cancer. The results indicate that the TC (OR = 2/992, CI = 95% 1/280-1/998) showed a significant relationship between the genetic diversity and breast cancer. The analysis of the combined CC and TC genotypes was associated with increased risk for breast cancer compared to TT genotypes (OR = 0/334, CI = 95%; 0.143-0.782, P = 0.009). Considering that the distribution of CC and TC genotypes was significant between the two groups of control and the patient, so the frequency of TT genotype with the same amount of P = 0.001 was significant between the two groups of control and the patient.
Conclusions: There was a significant relationship between the genotypes rs733618 polymorphism and breast cancer. However, there was no significant relationship between rs4553808 polymorphism and breast cancer risk.
