Parivash Kazemi, Habiballa Jowhary, Esfandiyar Sharifi, Akbar Zeraatpishe,
Volume 14, Issue 6 (1-2012)
Abstract
origanum vulgare l.spp viride was used in ancient medicine and it was medicated for digestive disease, diabetes, remedy of trauma. While antimicrobial, ant diabetic, anticancer and antioxidant effect of this plant was proved but there is done no study for its effect on reproductive system. Therefore the purpose of recent study is surveying eventual androgenic effect of this plant on hormonal level of pituitary - gonadal axis in mature male vistar rats.
Materials and Methods: recent study was done on five groups of male rats of vistar race and every group includes nine rats. The control group received no drug. The sham group received physiological serum and experimental groups of A, B and C were received the ethanolic extract of origanum vulgare l. with concentration of 40, 20, 10 mg/kg body weight respectively with gavaj for 14 days. Then levels of FSH, LH and TSH hormones in blood sample was measured with RIA method and obtaining result was compared between control group and other groups. Data was analyzed with SPSS software and statistical method of ANOVA and Tukey test. In this research, significant level was p<0.05.
Results: in control group, sham group and A, B and C groups, respectively mean and standard deviation from average of plasma concentration for LH hormone based on mlU /ml were: 0.18±0.006, 0.183±0.017, 0.187±0.026, 0.241±0.012 and 0.284±0.027 And for FSH Hormone were: 0.321±0.025, 0.342±0.071, 0.372±0.026, 0.383±0.031 and 0.372±0.026 And for TSH hormone were: 5.28±0.683, 6.07±0.5, 6.09±1.94, 6.66±1.48 and 8.1±1.66.
Conclusion: ethanolic extract of origanum vulgare leaf in maximum dose have androgenic effects and it can affect the activity of different levels of hypothalamic – pituitary – gonadal axis and increase the secretion of testosterone and gonadotropic hormones.
Saeed Hajihashemi, Razie Rajabi, Atefeh Ghiasabadi Farahani,
Volume 22, Issue 5 (11-2019)
Abstract
Background and Aim Gentamicin antibiotic has some side effects such as nephrotoxicity. The aim of this study was to evaluate the post-treatment effects of using hydroethanolic extract of Origanum Vulgare (OV) on nephrotoxicity caused by gentamicin.
Methods & Materials In this study, 32 male Wistar rats were divided into four groups of control (n=8), gentamicin (n=8; 100 mg/kg/day intraperitoneally for 8 days and gavage of distilled water for 2 days), OV extract group (intraperitoneal injection of normal saline for 8 days and using 40 mg/kg OV extract by gavage for 2 days), and gentamicin+ OV extract (intraperitoneal injection of gentamicin 100 mg/kg/day for 8 days and using 40 mg/kg OV extract by gavage for 2 days). The concentration of urea, creatinine, sodium, potassium and osmolarity were measured in plasma and urine samples. The right kidney was used to measure Malondialdehyde (MDA) and Ferric Reducing Antioxidant Power (FRAP).
Ethical Considerations This article was obtained from a research proposal approved by the Research Ethics Committee of Arak University of Medical Sciences (Code:IR.ARAKMU.REC. 1394.284)
Results Post-treatment administration of hydroethanolic extract of OV significantly decreased the concentration of urea, creatinine, absolute sodium excretion, relative sodium and potassium excretion, and MDA levels but significantly increased creatinine, urine osmolality and FRAP levels.
Conclusion Oral administration of OV extract as post-treatment method improved nephrotoxicity caused by gentamicin use by reducing oxidative stress of oxygen free radicals and lipid peroxidation in the affected kidneys.
