Showing 9 results for Nitric Oxide
Dr Ghasem Mosayebi, Mr Ali Ghazavi, Mr Mahmood Reza Khazaei, Mr Mohammad Ali Payani,
Volume 9, Issue 1 (3-2006)
Abstract
Introduction: Free radical-mediated peroxidation of biological molecules, such as lipids, is implicated in the pathogenesis of multiple sclerosis and it's animal model experimental allergic encephalomyelitis(EAE). Low concentration of antioxidant vitamin E has been observed in serum of multiple sclerosis. However, it is not known whether vitamin E has protective effect in EAE. Vitamin E may inhibit EAE by effect on the level of uric acid and Nitric Oxide (NO) production. Materials and Methods: In this experimental study some male C57BL/6 mice were placed in two therapeutic groups (n=8 per group) with age and weight-matched as follow: 1)Vitamin E-treated EAE mice (10mg/kg/every two days of vitamin E given i.p from day-3 until day+19 after disease induction, 2) Non-treated EAE mice (EAE control) received vehicle alone with same schedule. In addition, 5 age and weight-matched male C57BL/6 mice served as normal (non-EAE) controls. Clinical score of disease, uric acid and NO levels of the groups were analysed. Results: Results showed that vitamin E-treated mice had significantly less clinical score of EAE (4±0.8) than non-treated EAE induced mice (5.3±0.44), (p<0.01). Also, there was difference at the onset day of the disease between vitamin E-treated and non-treated EAE-induced mice (day 13±1 and day 11±1, respectively), although was not significant. Concentration of uric acid in vitamin E treated mice were significantly lower than EAE control (p<0.001). There was no difference at the level of NO between the groups. Conclusion: Vitamin E had no effect on NO level, but decreased serum uric acid level. It suggests that vitamin E can reduce or delay the onset of EAE by increasing uric acid consumption.
Mahmoudreza Palizvzn, Shadi Khademi, Ali Ghazavi, Ghasem Mosayebi,
Volume 9, Issue 4 (12-2006)
Abstract
Introduction: Oxidative stress may play a critical role in neurodegenerative disorders but the relation between oxidative stress and learning ability in normal rats is not investigated, so the aim of this study was to investigate the correlation between oxidative stress and two way active avoidance learning in Wistar rats. Materials and Methods: This is an experimental research. 14 Wistar rats were assigned for assessed learning ability in shuttle box. One day after shuttle box learning, cerebrospinal fluid (CSF) and blood samples were obtained. Concentration of Nitric Oxide and Ferric reduction/antioxidant power were assessed. Data was analyzed using Pearson correlation test. Results: The results of the present study demonstrate that there are positive correlation between shuttle box learning ability and Ferric reduction/antioxidant power (p<0.001, r =0.66 4) and Nitric Oxide concentration (p<0.001, r = 0.724) in serum, but not in CSF. Conclusion: The results of this study suggest that high concentration of antioxidant power and Nitric Oxide concentration in blood can improve shuttle box learning in rats
Kevan Ghasami, Ali Ghazavi, Yahya Jand, Mohamdali Payani, Mahmodreza Khazaei, Ghasem Mosayebi,
Volume 13, Issue 2 (6-2010)
Abstract
Background: Multiple sclerosis (MS) is a chronic, autoimmune disease of the central nervous system (CNS) of unknown etiology. Vitamin D3 (1,25(OH)2D3) has strong immune modulating potential. Nitric Oxide (NO) has been identified as one of the most destructive products of the immune system and is an important factor in demyelination. The effect of short-term vitamin D3 supplementation on NO level was assessed in MS patients. Materials and Methods: The study included 60 MS patients (male and female). Patients were randomized independently, in a double blind design, into one of two treatment groups. Controls (n=30) received current treatment. Vitamin D treated (n=30) individuals received current treatment plus 300000 IU vitamin D3. Vitamin D3 injection was repeated monthly for 6 months. Nitric oxide (NO) production was estimated by Griess reaction. Results: NO levels decreased following vitamin D3 treatment but the differences did not reach significance (12.411.1 μmol/L to 9.88.9 μmol/L). Control group was also associated with an increase in NO levels but not statistically significant (18.417.07 μmol/L to 22.0716.8 μmol/L). Conclusion: Vitamin D3 has not significant effect on the level of nitric oxide. However, further studies should be done to evaluate the potential of vitamin D as an immune modulator in management of MS.
Neda Soleimani, Saeed Daneshmandi, Morteza Sattari, Ali Akbar Pourfathollah,
Volume 13, Issue 4 (1-2011)
Abstract
Background: Correct use of cuminum cyminum and finding its different applications in medicine and industry necessitates a more clear understanding of this medicinal plant. This study deals with identifying the effects of the essential oil of this herbal plant on macrophages and tumor cell lines. Materials and Methods:Cuminum cyminum essential oil was extracted from its fruit, and its effects on peritoneal macrophages and LPS stimulated macrophages were examined. MTT assay was done for evaluation of macrophages viability and the amount of nitric oxide (NO) in culture supernant was measured by Griess Reagent. WEHI-164 mice fibrosarcoma cell line was cultured with different concentrations of cuminum cyminum and cytotoxicity level was evaluated by MTT assay. Results: The viability of macrophages and also, the amount of NO production in 50 and 500 μg/ml cuminum cyminum essential oil was lower than that of the control group (p<0.001). MTT assay showed that cuminum cyminum essential oil in 50 and 500 μg/ml concentrations significantly inhibits tumor cells growth (p<0.001). Conclusion: Cuminum cyminum essential oil by having immune-modulatory properties can be used in treatment of many inflammatory and immunologic disorders. Also, it can be used as a therapeutic or complementary agent in tumor therapy.
Zaman Arjmand Kolukhi, Zivar Salehi, Farhad Mashayekhi, Behrooz Najafi, Seyed Hossein Mirpoor,
Volume 17, Issue 8 (11-2014)
Abstract
Background: Nitric oxide is synthesized in endothelial cells by eNOS and acts as a pleiotropic regulator involved in carcinogenesis. Most gastric cancers develop from stomach epithelial cells therefore, NO may play a role in their development. Polymorphisms of eNOS have been shown to be associated with cancer susceptibility. In the present study, we investigated the association of the eNOS genotypes with gastric cancer risk in Guilan Population.
Materials and Methods: In this case-control study, we analyzed the Glu298Asp polymorphism of eNOS in 87 patients with gastric cancer and 90 healthy controls. The genotyping of eNOS polymorphism was performed using polymerase chain reaction–restriction fragment length polymorphism assays. All statistical analyses were conducted by the MedCalc statistical software.
Results: No association between the eNOS genotypes and gastric cancer risk was found. Among the 87 patients, 45 had Glu/Glu, 38 were Glu/Asp, and 4 were Asp/Asp. In the control group, 44 had Glu/Glu, 40 were Glu/Asp, and 6 were Asp/Asp. We found no significant differences in allele and genotype frequencies between control and patient specimens.
Conclusion: We found that there was no association between this polymorphism and gastric cancer risk. Results suggest that eNOS polymorphism may play a role in inhibition of gastric cancer. However, larger population-based studies are needed for clarifying the role of eNOS polymorphism in gastric cancer.
Farid Eghbali, Mahdi Moradi,
Volume 19, Issue 11 (2-2017)
Abstract
Background: High blood pressure increases various cardiovascular events about 2 to 3 times in a person. The purpose of this study is to recognize the effect of a course of pilates exercise on hypertension, nitric oxide, and resting heart rate in the eldrlymen with hypertension.
Materials and Methods: Subjects of this quasi-experimental research included 30 elderly men with hypertension grade one(in the range of systolic-diastolic 140/90 to 159/99mm Hg) who participated voluntarily and accessibly in the study and were divided randomly into two experimental peer (15 people) and control (15 people) groups. Weight, height and BMI of subjects were 75±80, 170±175 and 25-26, respectively. The experimental group did selected exercise of Pilates for eight weeks (three one-hour sessions per week). Desired variables of the subjects of both groups were evaluated 24 hours before the start of exercise and 24 hours after the last session. Data analysis was conducted using dependent and independent t-test by SPSS16 statistical software at the significant level of p≥0.05.
Results: The research results showed that the eight-week pilates exercise gave rise to reduction in blood pressure(p≤0.05) as well as resting heart rate (p≤0.05) and to increase in nitric oxide of elderly men with hypertension (p≤0.05).
Conclusion: The eight-week pilates exercise reduced blood pressure and resting heart rate and increased the production of nitric oxide in elderly men. So, it seems that the regular physical exercise can be effective as a preventive factor in the outbreak of cardiovascular diseases of the elderly men.
Ensieh Seif Amirhoseiny, Ali Ganji, Ghasem Mosayebi, Ali Ghazavi,
Volume 23, Issue 1 (3-2020)
Abstract
Background and Aim: Fennel with phytoestrogenic, antioxidant and anti-diabetic properties is used in traditional Iranian medicine to treat Polycystic Ovarian Syndrome (PCOS). Chronic inflammation is a new proposed mechanism in PCOS pathogenesis that suggests the role of immune system in this disease. Due to the main role of Treg cells in maintaining immune homeostasis, their impaired function, along with increased production of inflammatory compounds such as nitric oxide, can lead to persistent inflammation.
Methods & Materials: In this experimental study, 18 BALB/c mice were randomly divided into three groups including control group, untreated PCOS-induced group, and fennel-treated group. The control group received 0.1 ml sesame oil for 21 days. PCOS was induced by subcutaneous injection of Dehydroepiandrosterone (60 mg/kg/day) plus 0.1 mL sesame oil for 21 days. Mice in third group were treated by intraperitoneal injection of 500 mg/kg/day fennel extract after 21 days. The regulatory of T cells (Tregs) were analyzed by flow cytometry method and nitric oxide level determination was performed by Griess Reaction method. Ferric Reducing/Antioxidant Power (FRAP) assay was used to measure the total antioxidant power.
Ethical Considerations: This study was approved by the Ethical Committee of Arak University of Medical Sciences (Code: IR.ARAKMU.REC.1397.312).
Results: The estrous cycle was normalized after treatment with fennel. Fennel significantly reduced the number of ovarian cysts. Nitric oxide concentration decreased significantly while FRAP concentrations did not significantly alter. The number of Treg cells was also increased significantly.
Conclusion: Due to the role of immune system in the incidence of inflammation, concurrent modulation of both immune and endocrine systems may open new horizons in the design of new therapeutic interventions for PCOS.
Zahra Bahramnezhad, Ali Ghazavi, Ali Ganji, Ghasem Mosayebi,
Volume 24, Issue 3 (8-2021)
Abstract
Background and Aim: Medicinal plants contain various biological compounds, including phenols, flavonoids, and terpenoids, with anti-radical and anti-inflammatory activities that affect human health and improve life. Considering the numerous beneficial effects of Tanacetum Parthenium and Satureja Montana and the lack of full antioxidant capacity and anti-inflammatory effects of their combination, we decided to combine these two plants’ anti-inflammatory and antioxidant effects on induced acute inflammation in BALB/c mice.
Methods & Materials: In the present study, mice received the extracts of T. Parthenium and S. Montana and their combination by gavage for 14 consecutive days. Then, to induce acute inflammation, thioglycollate was injected intraperitoneally to all groups. FRAP and Grease tests were used to evaluate the total antioxidant capacity and serum nitric oxide concentration, respectively. One-way ANOVA analyzed the results.
Ethical Considerations: This study was approved by the Research Ethics Committee of Arak University of Medical Sciences (Code: REC.1398.021).
Results: The results showed that the combination of the extracts (P=0.006) and Satureja Montana (P=0.021) led to a significant increase in total antioxidant activity compared to the control group. Also, according to the results of grease test, Satureja montana (P=0.04), Tanacetum parthenium (P=0.034), and their combination (P=0.003) significantly reduced serum nitric oxide production compared to the control group.
Conclusion: The present study shows the synergistic effect of the combined extracts to increase their total antioxidant capacity and anti-inflammatory.
Sadegh Abbasian, Ali Asghar Ravasi, Rahman Soori, Sirous Choobineh,
Volume 25, Issue 2 (5-2022)
Abstract
Background and Aim Adropin is a new hormone plays an important role in improving lipid metabolism and vascular protection. The current study aims to determine the effect of eight weeks of high-intensity interval training (HIIT) on Adropin level and nitric oxide (NO) metabolites in overweight men.
Methods & Materials This is a quasi-experimental study with a pre-test/post-test design. Participants were 32 overweight men (Mean±SD age: 30.5±3.07 years, BMI: 27.93±4.2 kg/m2). They were randomly divided into two groups of HIIT (n=16) and control (n=16). The VO2max test was first conducted. Then, the HIIT group performed 8 weeks of HIIT (3 days per week) at 20 sessions of 30 seconds with a 13-s interval between each session. Body composition was measured using a bioelectrical impedance analyzer and the blood sampling was done for measuring Adropin and NO metabolites by ELISA kits in pre-test and post-test phases. The data analysis was done by repeated-measures ANOVA. The significance level was set at P<0.05.
Ethical Considerations This study was approved by the ethics committee of the University of Tehran (Code: IR.UT.REC.1395007). All procedures were in accordance with guidelines of the declaration of Helsinki.
Results There was a significant difference in Adropin between HIIT and control groups (0.88±0.256; 95% CI: 0.26-1.5 ng/mL). There was also a significant difference in NO metabolites between the two groups (7.5±3.03; 95% CI: 0.85-14.15 ng/ml). Moreover, there was a positive relationship between Adropin and NO metabolites in overweight men (r=0.498, P=0.0001).
Conclusion It seems that HIIT can attenuate cardiovascular diseases by increasing Adropin and NO and can improve cardiovascular fitness (VO2max) and body composition of men with overweight.
