Maryam Sadrnia, Ghasem Habibi, Mohammad Arjomandzadegan,
Volume 21, Issue 3 (6-2018)
Abstract
Background and Aim: In this study, the effect of Myrtus extracts on 25 methicillin resistant Staphylococcus aureus (MRSA) and Escherichia coli ESBL strains isolated from patients were compared by two methods.
Materials and Methods: 15 methicillin-resistant Staphylococcus aureus (MRSA) and 10 Escherichia coli ESBL isolates were used in this study. Fresh Leaves of Myrtus were collected from the herbal medicine farm. Extraction was performed using a reflux distillation. The effect of concentrations 0.195-100 micrograms per ml of Myrtus extract on clinical isolates was analyzed in disk diffusion method compared with micro broth dilution method and with MTT in 545 nm on an ELISA reader apparatus.
Findings: Inhibition zone diameter for the minimum effective concentration of 50 micrograms per milliliter in all isolates of ESBL and MRSA were as 8±1 mm and 11±1. Minimum Inhibitory Concentration (MIC) was 6.25mic/ml and Minimum Bactericidal Concentration (MBC) was determined 12.5mic/ml for E. coli ESBL. Furthermore, the amounts for MIC and MBC was determined as 12.5 and 25 mic/ml, respectively for Staphylococcus aureus.
Conclusion: The results of this study showed compliance of two methods in evaluation of drug-resistant clinical isolates. It was proved that the disk diffusion method could be determining range of effective concentration but micro broth method determines the effective concentration carefully. It is recommended that results obtained from disk diffusion not to be basis for final decisions in traditional medicine studies. Bacterial behavior in the broth and determination of the point of death greatly increases the accuracy of the results.
Fatemeh Jalalvand, Masoumeh Asle-Rousta,
Volume 21, Issue 3 (6-2018)
Abstract
Background and Aim: Chronic restraint stress impairs spatial learning and memory. Myrtle (Myrtus communis) has antioxidant, antidiabetic, hepatoprotective and neuroprotective properties. The aim of this study was to investigate the effect of myrtle hydroalcoholic extract on chronic restraint stress-induced spatial learning and memory deficit in adult male Wistar rats.
Materials and Methods: In this experimental study, 80 adult male Wistar rats were allocated to eight groups (10 in each) included control group (intact), myrtle0.75, myrtle1.5, myrtle3 groups (were gavaged with hydroalcoholic extract of myrtle at 0.75, 1.5 and 3 mg/ kg bw doses), stress group (restrained in restrainers for 6 hours per day for 21 consecutive days), stress- myrtle0.75, stress-myrtle1.5 and stress- myrtle3 groups (received myrtle extract at 0.75, 1.5 and 3 mg/ kg bw doses and exposed to chronic immobility stress). Spatial learning and memory were examined through the Morris water maze test.
Findings: Chronic immobilization stress caused spatial learning and memory impairment. Consuming doses 1.5 and 3 of Myrtle extract to stressed animals caused significant decrease in spent time and swam distance to reach the hidden platform (p<0.05) and increased time lapsed in target quadrant comparing with stress group (p<0.05).
Conclusion: It has been concluded that myrtle extract can improve spatial learning and memory in rats exposed to stress in a dose dependent manner.
Forough Khodadadnejad, Majid Akbari, Fateme Abdolalian, Mahboube Daneshvar, Behzad Ahmadi, Zahra Zahraei,
Volume 24, Issue 3 (8-2021)
Abstract
Background and Aim: The success of endodontic treatment depends on the complete elimination of pathogenic bacteria inside the root canal. Enterococcus faecalis, a gram-positive, pathogenic, and anaerobic cocci, are the primary etiology of root canal infection in treated and untreated teeth and are highly associated with endodontic treatment failure. This study aimed to compare the antimicrobial activity of Sodium hypochlorite 2.5%, microemulsion of Myrtus 10%, and microemulsion of Thyme 0.6% on the E. faecalis after root canal filling.
Methods & Materials: In this experimental study, 25 extracted single canal human anterior teeth were collected, and their crowns were cut. Then after washing and cleaning, and filling, the sterilization was done using an autoclave. Then, except 5 teeth (negative control group), the teeth were smeared with E. faecalis and then divided into 5 groups randomly represented the irrigation by Sodium hypochlorite 2.5%, microemulsion of Myrtus 10%, and microemulsion of Thyme 0.6%, with a positive control group and negative control group. The teeth were filled with gutta-percha and then incubated in an incubator for 90 days at a temperature of 37°C. Finally, the comparison of CFU in the groups due to abnormal data distribution was performed using the Kruskal-Wallis test.
Ethical Considerations: The Ethics Committee of Arak University of Medical Sciences approved this study (Code: IR.ARAKMU.REC.1398.297).
Results: According to the results of this study, the correlation between the groups of cultured teeth was not significant(P>0.05). The specimens washed with microemulsion of the Thyme 0.6% had the highest positive number of cultures; the specimens washed by Sodium hypochlorite 2.5% had the lowest number of cultures.
Conclusion: This study showed that no statistically significant difference exists in their antimicrobial activity after root canal therapy after the use of different wash solutions.
