Showing 24 results for Mellitus
Mohammad Ali Shariatzadeh, Ghasem Mosayebi, Naser Mahdavi Shahri , Ehsanollah Ghaznavirad, Ali Fani, Abdolrahman Dezfulian, Ali Chehreei ,
Volume 6, Issue 3 (10-2003)
Abstract
Introduction: Insulin-dependent diabetes mellitus (IDDM) or type 1 diabetes is an organ-specific autoimmune disease that caused by destruction of insulin-producing beta cells of the pancreas. Etiology of this disease is still unknown. It is seen that genetic and environmental factors play an important role for susceptibility to develop type 1 diabetes. The relationship between HLA associated factors and susceptibility to IDDM disease, was reported by several investigators. Also, some studies show that dermatoglyphics is associated with type 1 diabetes. However, it is maybe there is an association between HLA and dermatoglyphics inpatients'with type 1 diabetes and these characteristics could be applied for diagnosis of disease.
Materials and Methods: In this study, the prevalence of HLA (with using standard microlymphocytotoxicity method) and dermatoglyphics determined in 30 Iranian patients with IDDM and 30 normal healthy controls with similar ethnic background and the same geographical area.
Results: A significantly higher frequency of HLA-DQ, A2, DR3 and DQ2 were found in IDDM cases compared to the controls. The results obtain from dermatoglyphics showed that line ab was reduced in male and female type 1 diabetes. The reciever operating chractristics curve showed that the positive point for lines ab in right and left hands were 34.7 and 35.25, respectively.
Discussion: There is no association between HLA and dermatoglyphics.
With considering of genes encoding of HLA separated from genes determining dermatoglyphics, HLA typing and dermatoglyphics seem to be interesting tools for genetic studies related to type 1 diabetes. Further studies are recommended in order to provide more insight into the susceptibility to this disorder.
Dr Ghasem Mosayebi, Dr Ehsanola Ghaznavi Rad, Dr Ali Fani, Dr Seyedmohammad Moazenni,
Volume 8, Issue 3 (1-2006)
Abstract
Insulin dependent diabetes mellitus, human leukocyte antigens
Introduction: Insulin dependent diabetes mellitus (IDDM) or type 1 diabetes is created by autoimmune destruction of insulin-producing beta cells of the pancreas in genetically susceptible individuals. The relationship between human leukocyte antigens (HLA) associated factors and susceptibility to IDDM disease, was reported by several investigators. Association with different HLA types depends also on the studied populations. The aim of the present study was to determine HLA antigens which represent a high susceptibility to develop the IDDM disease in this area. Materials and Methods: In this study, the prevalence of HLA class-I and II antigens has been determined in 31 Arakian patients with IDDM and 57 normal healthy controls with similar ethnic background and from the same geographical area. The typing of HLA antigens was carried out using standard microlymphocytotoxicity method. Results: A significantly higher frequency of HLA-A2, A3, B21, DR3 and DQ2 were found in IDDM cases compared to the controls. In contrast, HLA-DR2, DR7 and B53 were represented at a somewhat higher frequency in controls compared to the IDDM patients. Conclusion: These results indicate that HLA-A2, A3, B21, DR3 and DQ2 antigens contribute to susceptibility to IDDM independently and HLA-DR2, DR7 and B53 antigens maybe associated with prevention of IDDM in Arakian patients.
Mehrdad Roghani, Tourandokht Balouchnejad, Samira Yadegari, Rouholah Miri,
Volume 9, Issue 3 (9-2006)
Abstract
Introduction: Considering the increasing incidence of atherosclerosis and cardiovascular disorders in diabetes mellitus, this study was conducted to evaluate the beneficial effect of two-month administration of Plantago Psyllium (PP) seed on the contractile reactivity of isolated aorta in diabetic rats. Materials and Methods: In this experimental study, 32 male Wistar rats were randomly divided into four control, PP-treated control, diabetic and PP-treated diabetic groups. To induce diabetes, Streptozotcin (STZ) was intraperitoneally administered (60mg/Kg). PP-treated groups received PP mixed with standard pelleted food at a weight ratio of 6.25%. After 2 months, contractile reactivity of thoracic aortic rings to KCl and Noreadrenaline were determined using isolated tissue setup. Data was analyzed using ANOVA and Tukey tests. Results: Serum glucose level showed a significant increase in diabetic group after one and two months (p<0.001), but it,s decrease in PP-treated diabetic group was not significant in comparison to diabetic group. Also PP-treated diabetic group showed a lower contraction to KCl (p<0.05) and noreadrenaline (p<0.01) as compared to diabetic group. Meanwhile, there was no significant difference between control and PP-treated control groups regarding contractile reactivity. Conclusion: It can be concluded that oral administration of PP for 2 months can decrease the contractile responsiveness of vascular system and this may prevent the development of hypertension in diabetic rats.
Mehrdad Roghani, Tourandokht Balouchnejad, Maryam Falahmohamadi,
Volume 10, Issue 2 (6-2007)
Abstract
Introduction: Use of medicinal plants for decreasing blood glucose and lipids to normal level is clinically important. In this respect, Silybum marianum (SM) is a plant that can lower lipid peroxidation and lipids in an experimental model of hyperlipidemia. Therefore, the effect of chronic oral administration of this plant on serum glucose, triglyceride, total cholesterol, and HDL and LDL cholesterol level of diabetic rats was investigated. Materials and Methods: In this experimental study, female rats (n=36) were randomly divided into 4 groups, including control, SM-treated control, diabetic, and SM-treated diabetic groups. The treatment groups received oral administration of plant-mixed pelleted food (6.25%) for 4 weeks. Serum glucose, triglyceride, total cholesterol, LDL and HDL cholesterol levels were determined before the intervention, and at 2nd and 4th weeks after the intervention. Data was analyzed using repeated measure ANOVA, One way ANOVA, student and pairt T and Tukey tests. Results: Serum glucose level in diabetic group was increased in seond and 4th weeks after the intervention as compared to one week before that (p<0.001) and SM treated diabetic rats only had a mild non-significant effect. In addition, triglyceride level in diabetic group increased 4 weeks after the intervention in comparison to related data one week before that (p<0.05) and there was a significant lower level of triglyceride in SM-treated diabetic rats (p<0.05). Furthermore, a similar significant reduction was obtained for treated-diabetic group as compared to diabetic group regarding serum cholesterol level (p<0.05). On the other hand, HDL- and LDL- cholesterol levels were significantly higher (p<0.05) and lower (p<0.05) in SM-treated diabetic group as compared to untreated diabetic group respectively. Conclusion: Oral chronic administration of SM had no significant hypoglycemic effect and led to appropriate changes in blood lipid profile.
Bahman Salehi, Mohammad Reza Rezvanfar, Faeze Shirian,
Volume 10, Issue 3 (6-2007)
Abstract
Introduction: Depression is one of the most common affective disorders, and in addition to different neurotransmitters and life events, various internal stressors such as blood glucose, cholesterol, triglyceride and other factors are responsible for it. Considering hyperglycemia as a possible risk factor of this disorder and inconsistency in results of previous studies, we decided to assess the relationship between different levels of HbA1C and major depression. Materials and Methods: This is a cross-sectional analythical study that is carried out on 134 samples. They were chosen randomly among patients with type2 diabetes reffering to Vali-e-Asr hospital. The patients were divided into 2 groups on the basis of their HbA1C test results patients with HbA1C less than 7% (group A), consisting 55 persons and patients with HbA1C more than 7% and equal to it (group B), including 79 persons. Participants were assessed by Hamilton scale of depression. The relation between HbA1C levels and major depression in these 2 groups was assessed using Chi square and Mann Whitney U tests. Results: We found that 40% of depressed patients had HbA1C level lower than 7% and 60% of them had HbA1C level higher than 7%. No significant correlation was found between HbA1C and major depression. But the duration of disease, using Insulin and being hypertensive simultaneously was correlated to major depression in this study. Conclusion: This study indicates that uncontrolled diabetes and high level of blood glucose is cosidered of less importance as an undelying mechanism of depression in patients with type 2 diabetes.
Mehrali Rahimi, Efat Mashhadi, Ebrahim Karimi,
Volume 10, Issue 3 (6-2007)
Abstract
Introduction: Diabetes mellitus is a chronic disease that has well known effects on most organs, but there have been controversies in few different studies about the effects of diabetes on hearing. So this study was carried out in 2003-2004 in Kermanshah Diabetes Research Center to assess the effect of diabetes on hearing. Materials and Methods: This is a case-control study. 82 diabetic cases (26 types I and 56 type 2 cases) were studied along with 82 controls matched both in age and sex. Cases were selected randomly, their range of age was 15 to 55 years and their duration of diabetes was more than 3 years. After completing questionnaires, all cases and controls undergone autoscopic examination. Those with other ear disease with hearing impairment were omitted. Audiometery including pure tone audiometery, speech audiometery, and impedance audiometrey with well tone AD19 formed was done on 164 (diabetic and control) persons. Data was analyzed using t-test. Results: Audiometric findings with different frequencies in the two groups revealed that there was a significant relationship between diabetes and hearing impairment (p<0.001). This relationship was more significant in high frequencie, older age and type 2 diabetes, but there were no relationship between control of blood sugar (HbA1c), duration of diabetes, sex, microvascular and macrovasculur complications with hearing loss in diabetic patients. Conclusion: These finding demonstrate that diabetes can cause mild to moderate hearing impairment especially in high frequencies. The mechanism of this effect is unknown. Complementary studies with more patients and with more accurate devices such as Otoacustic Emission (OAE) and Audiometric Brainstem Reflex (ABR) are recommended
Alireza Shahab Jahanlou, Fazlallah Ghofranipour, Alireza Sobhani, Masoud Kimmiagar, Maryam Vafaei,
Volume 11, Issue 2 (6-2008)
Abstract
Introduction: Quality of life (QOL) has become an important concept in medical research. Some research has shown a curvilinear relationship between QOL and glycemic control, but some did not so. The aim of this study is to investigate different aspects of QOL and also assess the hypothesis in diabetic patients. Materials and Methods: In a cross-sectional study, a random sample of adult diabetic outpatients (n=110) were selected and completed the WHO-QOL assessment instruments. In addition HbA1c was measured in these patients by calorimetric method. Patients were divided in to 3 groups: 1-good control (HbA1c<7), 2-fair control (7≥HbA1c<9) and 3-poor control (HbA1c≥9). QOL was assessed by measuring physical, psychological, social and environmental domains. Data were analyzed with one way ANOVA (Tukey as post hoc), Chi2 and Mann-Whitney, and correlation tests. Results: A significant correlation was seen between BMI and glycemic control, physical and social aspects, physical and environmental aspects, psychological and environmental aspects, and social and psychological aspects. No significant difference was seen between mean score of different dimensions of QOL in relation to HbA1c changes. The mean score of each dimension in relation to glycemic control showed that, better score of psychological, environmental and social dimensions is seen in patients with fair glycemic control. But regarding the overall score of QOL this is completely reverse. The mean score of physical dimension is decreased with increasing HbA1c. There was no correlation between other domains of QOL and glycemic control. Conclusion: In this survey higher quality of life was seen in fair glycemic control in social, environmental and psychological dimensions, but physical dimension in patients was decreased when HbA1c was increased. Regarding the overall score of QOL it can be seen that the lowest score of QOL is in patients with fair glycemic control which is in contrast with curvilinear hypothesis.
Маryam Tehranipour, Jina Khayyatzade, Zahra Ghorbani,
Volume 12, Issue 4 (2-2010)
Abstract
Background: Maternal diabetes is associated with an increased risk of several complications in the offspring, such as the growth of congenital disturbances and malformations. In the present study, we examined the effects of maternal hyperglycemia on the volume of brain and lateral ventricle in newborn Wistar rats. Materials and Methods: In this experimental study, on the 7th day of pregnancy, hyperglycemia was induced by a single injection (i.p.) of streptozotocin (55 mg kg-1) and the animals in control group were given an equal volume of citrate buffer. After parturition on the first day of birth, a litter was randomly selected from each mother, their brains dissected and fixed in 10% formalin. Then they were sectioned with 7μm thickness and stained by H.E. Through the use of stereological techniques and systematic random sampling scheme, the volume of the brain and lateral ventricles were estimated. Results: Statistical analyses showed significant increases (p<0.05) in the volume of the brains and lateral ventricles in the experiment group in comparison to the control group. Conclusion: By affecting blood brain barrier permeability in newborn rats, maternal diabetes can generate a large amount of CSF. These effects can bring about brain disorders such as hydrocephalus.
Faramarz Fallahi, Mehrdad Roghani, Majid Khalilizad,
Volume 12, Issue 4 (2-2010)
Abstract
Background: Considering some evidence on anti-diabetic potential of Allium ursinum (AU) , this study was conducted to evaluate the effect of oral administration of AU on contractile responsiveness of thoracic aorta in diabetic rats.
Materials and Methods: In this experimental study, 40 male Wistar rats were divided into control, AU-treated control, diabetic, glibenclamide-treated, and AU-treated diabetic groups. For inducing diabetes, streptozotcin (STZ) was administered (60 mg/Kg). AU-treated group received AU mixed with standard pelleted food at a weight ratio of 1% for 2 months. Serum glucose level was measured at weeks 4 and 8. Eventually, contractile responsiveness of thoracic aortic rings to KCl and noradrenaline (NA) was evaluated .
Results: Serum glucose level, at weeks 4 and 8, in the AU-treated diabetic group was significantly lower than that in the diabetics group (p<0.01 and p<0.005, respectively). In addition, the maximum thoracic aorta contractile responsiveness to NA in the AU-treated diabetic group was significantly less than the diabetic group (p<0.05) however, such a significant reduction was not observed for KCl.
Conclusion: Oral administration of AU for 2 months is of a moderate hypoglycemic effect and attenuates the contractile responsiveness of the vascular system in diabetic rats. Background: Considering some evidence on anti-diabetic potential of Allium ursinum (AU), this study was conducted to evaluate the effect of oral administration of AU on contractile responsiveness of thoracic aorta in diabetic rats. Materials and Methods: In this experimental study, 40 male Wistar rats were divided into control, AU-treated control, diabetic, glibenclamide-treated, and AU-treated diabetic groups. For inducing diabetes, streptozotcin (STZ) was administered (60 mg/Kg). AU-treated group received AU mixed with standard pelleted food at a weight ratio of 1% for 2 months. Serum glucose level was measured at weeks 4 and 8. Eventually, contractile responsiveness of thoracic aortic rings to KCl and noradrenaline (NA) was evaluated. Results: Serum glucose level, at weeks 4 and 8, in the AU-treated diabetic group was significantly lower than that in the diabetics group (p<0.01 and p<0.005, respectively). In addition, the maximum thoracic aorta contractile responsiveness to NA in the AU-treated diabetic group was significantly less than the diabetic group (p<0.05) however, such a significant reduction was not observed for KCl. Conclusion: Oral administration of AU for 2 months is of a moderate hypoglycemic effect and attenuates the contractile responsiveness of the vascular system in diabetic rats.
Shahnaz Shahrjerdi, Nader Shavandi, Rahman Sheikh Hoseini,
Volume 12, Issue 4 (2-2010)
Abstract
Background: Diabetes Mellitus is a heterogeneous group of different metabolic disorders that are characterized by chronic increase of blood glucose and proteins, lipids and carbohydrates metabolism disorder. The aim of this study was to investigate the effect of 8 weeks of aerobic exercise on metabolic factors, quality of life and mental health of women with type II diabetes. Materials and Methods: In this semi-experimental investigation, 18 women with type П diabetes referring to Arak Petroleum Company Clinic were selected and divided into two groups: aerobic (10 participants) and control (8 participants). Aerobic group performed selected exercises in 8 weeks (three 30-50 min sessions per week). In this investigation, we used metabolic factors (such as total cholesterol, triglycerides, HDL, VLDL, LDL, FBS, HbA1c and Insulin serum) and SF-36 quality of life (QOL) and GHQ mental health (MH) questionnaires. The level of significance was considered to be 95% (P<0/05). Results: There were significant differences between the means of pre-test and post-test measures of FBS, HbA1C, Insulin serum, QOL and MH in aerobic group. There were no significant differences between the means of pre-test and post-test measures of TC, HDL, LDL, VLDL and triglycerides in aerobic group and all factors in the control group (P<0/05). Conclusion: Aerobic exercise affects metabolic factors, QOL and MH in type П diabetic patients and it results in their improvement.
Roghani, Khalili, Baluchnejadmojarad, Heydari,
Volume 14, Issue 1 (3-2011)
Abstract
Background: Chronic diabetes mellitus is accompanied with disturbances in learning, memory, and cognitive skills. Noticing the existing evidence regarding the anti-diabetic potential of hesperetin, the effect of its chronic administration on learning and memory in diabetic rats was investigated. Materials and Methods: In this experimental study, 40 male Wistar rats were divided into control, hesperetin-treated control, diabetic, and hesperetin/glibenclamide-treated diabetic groups. For evaluation of learning and memory, initial (IL) and step-through latencies (STL) were determined at the end of the study using passive avoidance test, and the alternation behavior percentage was obtained using Y maze. Results: STL significantly decreased in the diabetic (p<0.01) and hesperetin-treated diabetic (p<0.05) groups in comparison to the control group however, the difference between these two groups was not significant. Alternation percentage in the diabetic group was significantly lower in comparison to the control group (p<0.05), but the hesperetin-treated diabetic group revealed a significant difference in comparison to the diabetic group (p<0.05). Conclusion: Although long-term treatment with hesperetin does not enhance the capability of retention and recall in diabetic animals on the passive avoidance test, it can improve the short-term spatial memory in diabetic animals.
Navid Mazruei Arani, Homa Baghaei, Ashraf Khorrami Rad, Zatollah Asemi, Sabiheosadat Alizadeh, Ahmad Esmaeilzadeh,
Volume 16, Issue 6 (9-2013)
Abstract
Background: Increased insulin resistance, inflammatory factors and parameters of oxidative stress are associated with the development of diabetes complications. This study was designed to determine the beneficial effects of synbiotic Gaz on insulin resistance, inflammatory factor and oxidative stress in patients with type 2 diabetes.
Materials and Methods: This randomized crossover double-blinded controlled clinical trial was performed among 62 diabetic patients aged 35-70 y. Subjects were randomly assigned to consume of either the synbiotic (n=62) or control Gaz (n=62) for 6 weeks. A 3-week washout period considered. The synbiotic Gaz was consisted of a probiotic viable and heat-resistance strain Lactobacillus sporogenes (1×107 CFU), 0.04 g inulin and 0.05 g stevia per 1 g as sweeteners' substances. Control Gaz (the same substance without probiotic bacteria and prebiotic inulin) was. Patients were received synbiotic and control foods in a 7 g package thrice a day. Fasting blood samples were taken at baseline and after 6-week intervention to measure insulin resistance, hs-CRP and biomarkers of oxidative stress.
Results: Consumption of synbiotic Gaz, compared with control, resulted in a significant decrease in serum insulin (p=0.02) and hs-CRP levels (p=0.008). Supplementation with synbiotic Gaz led also to a significant increase in plasma total glutathione (p<0.0001) compared to the control.
Conclusion: In conclusion, consumption of synbiotic Gaz for 6 weeks resulted in decreased levels of serum insulin, hs-CRP and increased levels of plasma total glutathione.
Mohammad Ahmadzadeh, Jamshid Faraji,
Volume 16, Issue 11 (2-2014)
Abstract
Background: Wolfram syndrome is a rare neurodegenerative autosomal recessive disorder. That occurs in association with diabetes mellitus, diabetes insipidus, and optic nerve atrophy, hearing loss, urinary tract infection and nervous complication.
Case: The present report belong to a 24 years old girl who was effected by diabetes mellitus in 10 years old & has been effected by Diabetes insipidus, recurrent UTI, hearing loss and other type of disorders in 20 years old.
Conclusion: Strict control of blood glucose and regular fundoscopy, for early detection of optic atrophy are important. Step in management of this syndrome and quality of life is improved with hearing and vision aid tools.
Malihe Aveseh, Rohollah Nikooie, Vahid Sheibani ,
Volume 17, Issue 5 (8-2014)
Abstract
Background: Considering to the frequency of occurrence of hypoglycemia in diabetes, alternative substrates for glucose play an important role in maintaining brain metabolism. The aim of the present study was to investigate the long-term effects of endurance training on brain lactate uptake during hypoglycemia in type 1 diabetic rats.
Materials and Methods: In this experimental study, seventy-two male Wistar rats were equally divided into four groups: Control, Trained, Diabetic control and Diabetic trained. Diabetes was induced by intraperitoneally injection of streptozotocin. After 8 weeks of endurance training, Hypoglycemia was induced by an intraperitoneally injection of insulin. One-half hours after hypoglycemia, lactate (4 mmol/kg) was injected intraperitoneally into animals. Animals were killed at intervals of 10, 20, and 30 min after injection, plasma and brain concentration of lactate and glucose were measured.
Results: A significant difference was found for plasma lactate concentration at intervals 20 (p<0.05) and 30 (p<0.05) between healthy groups and also between diabetic groups (p<0.05). Thirty minutes after injection, significant increase in brain lactate concentration was found between control and trained (p<0.05) and control diabetic and diabetic trained (p<0.05). Brain/plasma ratio of lactate had a similar pattern with brain lactate concentration. Increase in brain lactate concentration had no effect on decreased level of brain glucose concentration.
Conclusion: Endurance training can increase the brain lactate uptake of diabetic rats during hypoglycemia.
Elnaz Vaghef Mehrabani, Leila Vaghef Mehrabani, Sharareh Norouzi, Somayyeh Ziyadi, Aziz Homayouni Rad,
Volume 18, Issue 9 (12-2015)
Abstract
Background: Metabolic syndrome, a concurrence of overweight and abdominal fat distribution, disturbed glucose and insulin metabolism, mild dyslipidemia and hypertension is among the most important issues that man deals with, in today's world. Probiotics are live microorganisms which, when administered in adequate amounts, can confer a health benefit on the host. The aim of the present study was to review the clinical evidence available regarding the role of probiotics in improvement of disorders associated with metabolic syndrome.
Materials and Methods: In this research, clinical trials performed between 2000-2014 were searched in two databases, Pubmed and ScienceDirect. In both databases “Probiotics”, “metabolic syndrome” and each of its elements were used as the key words.
Results: Probiotics may reduce body weight through manipulating the gut flora. Through reducing inflammation and immune-regulation, probiotics decrease insulin resistance. They also improve lipid profile through reducing cholesterol absorption and producing short chain fatty acids, as well as deconjugating bile acids and converting cholesterol to coprostanol. Moreover, probiotics have been suggested to have a hypotensive effect through release of angiotensin-converting enzyme inhibitory peptides, through proteolytic action.
Conclusion: Although some clinical trials have failed to obtain beneficial effects of probiotics on metabolic syndrome related situations, a majority of the studies have shown positive results for these microorganisms in this regard. Thus, probiotics can be looked upon as a novel approach when either prevention or alleviation of metabolic syndrome is aimed.
Mohammad Parastesh, Ali Heidarianpour, Mohammad Bayat, Abbas Saremi,
Volume 19, Issue 8 (11-2016)
Abstract
Abstract
Background: Diabetes mellitus is associated with reductions in fertility indices. Resistance training, on the other hand, through reducing the adverse effects of diabetes, exerts a positive impact on diabetic individuals. The aim of the present study was to examine the effects of ten weeks of resistance training on serum levels of reproductive hormones and sperm parameters in Wistar rats with diabetes mellitus type 2.
Materials and Methods: In this experimental study, 36 Wistar rats with mean weight of 200±50 were randomly assigned to healthy control, diabetic control and diabetic training groups. The diabetic resistance training group received ten weeks of resistance training (climbing up the ladder) following the induction of diabetes. Twenty-four hours after the last training session, left epididymis of the rats was examined for studying sperm parameters and blood serum samples were examined for evaluating reproductive hormones. Data were analyzed using one-way ANOVA and Turkey’s Post Hoc test at 0.05%.
Results: Ten weeks of resistance training induced significant increases in serum testosterone and FSH levels in the resistance training group in comparison to the diabetic group (p<0.007).Resistance training did not have any significant effects on serum LH levels in the resistance training group compared to the diabetic control group. In addition, sperm parameters (sperm count, survival rate and motility) presented significant improvements compared to the diabetic group(p<0.05).
Conclusion: Resistance training can improve sperm parameters, including sperm count, survival rate and motility, through increasing serum testosterone, LH and FSH levels (reproductive hormones) in rats with diabetes mellitus type 2.
Mehri Jamilian, Jamilian Somayeh Jamshidi,
Volume 20, Issue 10 (1-2018)
Abstract
Abstract
Background: Selenium supplement has multiple important effects, including anti-inflammatory effect. The aim of this study was to assess the effects of selenium supplement on gene expression of inflammatory cytokines and vascular endothelial growth factor in gestational diabetes.
Materials and Methods: This randomized double blind placebo control trial was performed on 40 patients suffering from GDM aged 18–40 years old. Participants were randomly divided into interventional group receiving 200mg/day selenium supplements (n=20) and control group receiving placebo (n=20) for 6 weeks. Primary outcome was gene expression of inflammatory cytokines and VEGF which were assessed in lymphocyte of GDM patients by RT-PCR method.
Results: After 6 weeks intervention, in comparison with the control group, interventional group showed down regulation of gene expression of tumor necrosis factor alpha (TNF–α) (p=0.02) and transforming growth factor beta (TGF–β) (p=0.01) and up-regulation of gene expression of vascular endothelial (VEGF) (p = 0.03) in lymphocytes of GDM. There was not any significant change following intervention with selenium regarding gene expression of interleukin IL-1 β and IL-8 in lymphocytes of GDM patients.
Conclusion: 6 weeks supplementation with selenium in patients with GDM can cause down regulated gene expression of TNF-α and TGF–β, and up regulated gene expression of VEGF. Selenium supplement had not any effect on gene expression of IL-1 β and IL-8.
Mohammad Parastesh, Nader Shavandi,
Volume 21, Issue 2 (5-2018)
Abstract
Abstract
Background: Diabetes mellitus is associated with reductions in fertility indices. Interval training, on the other hand, through reducing the adverse effects of diabetes, exerts a positive impact on diabetic individuals.The aim of the present study was to examine the effects of ten weeks of High Intensity Interval Training (HIIT) on reproductive hormones and sperm parameters in Wistar rats with diabetes mellitus type 2.
Materials and Methods: In this experimental study, 36 Wistar rats with mean weight of 200±48 were randomly assigned to healthy control, diabetic control and diabetic + high intensity interval training groups. The diabetic training group received ten weeks of HIIT training by treadmill following the induction of diabetes. Twenty-four hours after the last training session, left epididymis of the rats was examined for studying sperm parameters and blood serum samples were examined for evaluating reproductive hormones. Data were analyzed by one-way ANOVA and Tukey's post hoc test at a significant level of 0.05%.
Results: Ten weeks of HIIT training reduces fasting blood glucose (p=0.001) and significantly increases serum testosterone (p=0.001), LH (p=0.042) and FSH (p=0.024) levels in the HIIT training group in comparison to the diabetic group. In addition, sperm parameters (sperm count, survival rate and motility) presented significant improvements compared to the diabetic group (p<0.05).
Conclusion: It seems that HIIT training can improve sperm count, survival rate and motility, through increasing serum testosterone, LH and FSH levels (reproductive hormones) in rats with diabetes mellitus type 2.
Farzaneh Rooshenas, Mahboobeh Ashrafi, Saeed Nazifi, Mahmoud Aminlari, Sara Talebanzadeh,
Volume 21, Issue 5 (10-2018)
Abstract
Background and Aim: Medicinal plants possessing antioxidant activity may reduce oxidative stress and improve the functions of various organs that affected by hyperglycemia. The aim of the present study was to evaluate the effects of saffron aqueous extract (SAE) administration to diabetic rats by measuring the oxidative stress parameters and important biochemical enzymes in liver tissue.
Materials and Methods: 72 hours after STZ administration (60 mg/kg body weight), the animals with fasting blood glucose over of 250 mg/dl were considered to be diabetic rats and experimental groups were: control (1), control drug (2), diabetes (3) and diabetes drug (4). The treatment was started on the 7th day after STZ injection with i.p injection of SAE (200mg/kg body weight), five doses and weekly to groups 2 and 4. At the end of the experimental period, biochemical factors were measured after bleeding and harvesting of tissues.
Findings: Results indicated the perturbation in the activity of important liver enzymes in diabetic group (3) and SAE adjusted and normalized their levels activity. In addition, SAE with increases in the activity of antioxidant enzymes alleviated diabetes induced oxidative stress and thus reduced MDA levels in group 4 compared to group 3.
Conclusion: SAE is not only useful in the controlling of blood glucose, but also has antioxidant potential to protect the liver tissue of diabetic rats against damage caused by hyperglycemia-induced oxidative stress.
Mohammad Amin Edalatmanesh, Habibollah Khodabandeh, Nooshin Yazdani, Samaneh Rafiei,
Volume 21, Issue 6 (12-2018)
Abstract
Background and Aim: Neuropathy is the most common abnormality in diabetes mellitus which characterized with cerebral damages especially in hippocampus. This study evaluates the effect of Cinnamomum Zeylanicum extract (CZE) on memory, hippocampal neuron damage and antioxidant enzymes levels in animal model of diabetes.
Materials and Methods: 50 adult Sprague dawley rats were randomly divided into 5 groups: Control, STZ (Streptozotocin, 50 mg/kg; i.p.), and STZ + CZE100, STZ + CZE200 and STZ + CZE400 which were treated with CZE in 100, 200 and 400 mg/kg, respectively. CZE was administered in 14 days, orally. After evaluation of working and spatial memory, activity of catalase (CAT) and glutathione peroxidase (GPx) enzymes was assessed by ELISA. Then, histopathological assessment of hippocampus was done.
Findings: In comparison with the controls, STZ group showed an increase in latency time and distance to the hidden platform in MWM, a decrease in alteration behaviors, cell density and activity of CAT and GPx enzymes in hippocampus (p˂0.05). In addition, treatment with CZE decreased latency time and distance in MWM and increased alteration behavior, hippocampal cell density and activity of antioxidant enzymes in comparison with the STZ group (p˂0.05).
Conclusion: Diabetes with reduction of neuronal density and activity of antioxidant enzymes in the hippocampus causes deficits in spatial and working memory. However, Administration of CZE ameliorates these neuropathologic disorders.
