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Effects of disinfecting meatus and urinary catheter with 10% Povidone Iodine ointment on incidence rate of bacteriuria in hospitalized male patients in neurologic wards
Kurush Basami, Zahra Mahdavi, Malahat Nikravan Mofrad, Hamidreza Kohestani, Nayyereh Baghcheghi,
Volume 11, Issue 2 (6-2008)
Abstract
Introduction: Urinary tract infection is one of the most common nosocomial infections that mainly occur after instrumentation in urinary tract, particularly after urinary catheterization. This study was conducted to assess disinfection's effects of meatus and urinary catheter with 10% Povidone Iodine ointment on incidence of bacteriuria in hospitalized patients of neurological unit of Shohadaye Tajrish hospital in iran. Methods and Materials: This study , is a randomized clinical trail which is done on Seventy stroke's patients that were catheterized by urologic expert's selected on convenience sampling and randomly divided to tow groups (Experimental group: 35 patients, Control group: 35 patients). In the experimental group, meatus and catheter drainage tube junction were disinfected daily and spigot was disinfected tow times a day by PVP10% ointment. In the control group the cares were done by normal saline. In days: 2,5,8,11,14 a urine sample was sent to lab for culture. The maximum period of study was 14 days. Results: Incidence of bacteriuria in experimental and control groups were 20% and 42.9%, respectively. Relative risk was 2.14. In patients with positive urine cultures: puri was 85.7% in experimental group and 86.6% in control group. Clinical symptoms of UTI were seen in 28.6% in experimental group and 33.3% in control group. Conclusion: This study showed that application of povidone-iodine 10% ointment for disinfection meatus and catheter drainage tube junction is effective to prevent catheter-associated urinary tract infection in male patients.
Isolation of Listeria Monocytogenes from Meat Products and Study on PrfA Gene in Bacteria Isolated and Compaire Them with Clinical Samples and Standard Samples
Jamileh Nowroozi, Elham Siasi Torbati, Robab Baharvand,
Volume 16, Issue 9 (12-2013)
Abstract

Background: Listeria is a gram positive bacterium, facultive intracellular pathogene. Prf A gene has an important role in virulence. The purpose of this study was to determine of the presence of Listeria monocytogenes in various meat products and presence of prf A gene in bacteria isolated from food samples and compare them with clinical samples and standard samples.

Materials and Methods: During 6 months of 60 samples of beef, chicken, ham, cocktail, sausage, from a butchery’s Khoramabad and shops in Khoramabad and Tehran were collected. L. monocytogenes was isolated according to cold enrichment method and prf A gene were analyzed by PCR (polymerase chain reaction) method. Statistical analysis was performed with the software SPSS (statistical package for social science).

Results: From 60 samples of meat and meat products, 8 (13.3%) were positive for Listeria spp. Among in these samples, 4 cases of L. monocytogenes (6.6%), 3 cases (5%), L. innocua and 1 case (1.6%) L. welshimri, were isolated which L. innocua was isolated from meat and poultry samples and L. monocytogens from meat, chicken, ham and L. welshimeri from meat were isolated. Prf A gene was detected in isolated L. monocytogenes and donated bacteria from dairy products, clinical and standard samples, 2 cases of donated samples of vegetable contained prf A genes.

Conclusion: The prf A gene (activator of transcription and regulators the expression of other virulence genes), in the studied samples were observed. This gene plays a role in pathogenesis. Because these bacteria are transmitted through food and is a serious threat to public health. Thus identification of bacteria especially its genes could be possible to find some ways to prevent the bacteria and avoid disease from it.


Optimization of a Multiplex PCR for Simultaneous Detection of Foodborne Pathogens Salmonella spp. and Escherichia coli O157:H7 and Contamination Prevalence Assay in Meat Products
Hossein Morsali, Golnaz I Asaadi Tehran, Hanieh Asaadi, Sajjad Yazdansetad, Reza Najafpour,
Volume 20, Issue 6 (9-2017)
Abstract
Abstract
Background: Salmonella spp. and Escherichia coli O157:H7 are the most common bacterial foodborne pathogens contaminating food products especially meat. It is essential to detect the pathogens rapidly, specifically, and simultaneously by selection and optimization of suitable reference genes. The present study was conducted to simultaneously detect E. coli O157:H7 and Salmonella spp. in meat products and contamination prevalence assay by using multiplex PCR based on rfbE and invA genes amplification in Zanjan province, northwest of Iran.
Materials and Methods: A total of 74 meat samples were obtained from various regions of Zanjan province, randomly. 25 grams of each meat sample was completely homogenized in 225 ml of Mueller-Hinton broth growth medium and incubated. Bacterial strains were purified and DNA extraction was performed from purified bacterial isolates. Simultaneous amplification of rfbE and invA gene fragments was done with specific primers by optimization of a multiplex PCR. Finally, the sensitivity of the method was evaluated by inoculation of the bacteria to the meat.
Results: Out of 74 meat samples, 6(8%), 4(5.4%), and 2(2.7%) samples were positive for E. coli O157:H7, Salmonella, and both E. coli O157:H7-Salmonella, respectively. Multiplex PCR indicated high sensitivity for simultaneous detection of the pathogens in lowest dilution of the bacteria that had been inoculated to the meat.
Conclusion: In this study, a multiplex PCR was optimized based on Salmonella spp. and E. coli O157:H7 virulence genes for rapid and simultaneous detection of the pathogens with high sensitivity and specificity. Multiplex PCR as a reliable tool for rapid and simultaneous detection of foodborne pathogens to prevent contamination of food products.
 

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