Journal of Arak University of Medical Sciences

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Showing 6 results for Klebsiella

Comparative Study on the Effects of Aloe Vera Extract in Clinical Strains of Staphylococcus Aureus, Klebsiella, Staphylococcus Epidermidis and Escherichia Coli Compared to Antibiotics of Choice

Maryam Sadrnia, Mohammad Arjomandzadegan,
Volume 17, Issue 6 (9-2014)

Abstract

Background: Nowadays, with the development of drug resistance, the use of herbs as an alternative to chemical drugs is considered by researchers. In this work, effects of Aloe vera extracts on clinical isolates was studied.

Materials and Methods: Aloe vera plant medicinal plants were obtained from a greenhouse. Three extracts including essential oils, extracts and no essential oils and essential oil extraction method also includes a complete extract of Aloe vera were prepared Percolation total. To investigate Microbiology extracts of two strains of Staphylococcus aureus and Staphylococcus epidermidis clinical strain of Gram-positive and Gram-negative isolates of Klebsiella pneumoniae and Escherichia coli strains Staphylococcus aureus ATCC25923 were used as well. Evaluate the effect of two methods: Kirby-Bauer disk with the minimum inhibitory concentration (MIC) was performed using microplate dilution. Turbidity was determined by an ELISA reader apparatus.

Results: All extracts of aloe vera on Klebsiella with a diameter of 32±2 mm mg/ml 285.7 concentration with microplate dilution method was 2.23 mg/ml. Staphylococcus aureus and MIC zone diameter of 30±2 mm and mg/ml 2.23, Staphylococcus epidermidis and Escherichia coli mg/ml4.46 mm 17.85 mm 30±5 mg/ml 17.85 respectively. Similar concentration of 17.85 mg ml Aloe Vera with a circle formed by the disk mc/ml 10 gentamicin was shown. This effect is similar to other bacteria antibiotics gentamicin, clindamycin, erythromycin, and Cefixime compared with Aloe Vera extract has been proven. Essential oils made from all parts of the same whole extract of aloe vera, but not essential extracts, bacteria studied were ineffective.

Conclusion: In this study the effects of similarity and some excess water Asrsarh Aloe Vera with common antibiotics on bacteria causing the infection was confirmed. Therefore, by production of appropriate pharmaceutical plant drugs with fewer side effects, bacterial infections couled be treated properly.

The Frequency of Extended Spectrum β-Lactamase Genes of SHV-2a, SHV-5 and SHV-12 in Clinical Isolates of Klebsiella Pneumoniae Isolated from Kermanshah Medical Centers in 2014

Mohammad Hosein Feiz Sarshar, Alisha Akya,
Volume 19, Issue 2 (5-2016)

Abstract

Background: The dissemination of extended-spectrum β-lactamases (ESBL) in Klebsiella pneumoniae isolates has resulted in the increase of antibiotic resistance and mortality among patients. The aim of this study was to determine the prevalence of ESBL and SHV-2a, SHV-5 and SHV-12 genes in K. pneumoniae isolates from Kermanshah.

Materials and Methods: In this descriptive – analytical study, from 112 clinical samples of patients admitted at Kermanshah medical centers in 2014, 60 K. pneumoniae isolates were recognized by standard methods of bacteriology and API Kit. Antibiotic susceptibility of isolates was determined by disk diffusion method and the isolates were screened for ESBL-producerig using the combination disc method. The SHV-2a, SHV-5 and SHV-12 genes were determined among isolates using PCR method. Primers were designed in this study.

Results: Of 60 isolates tested, the highest and the lowest resistance was for ampicillin and carbapenem antibiotics, respectively. Forty-five percent of isolates were ESBL-producer. Among 60 isolates tested, 5 (8.3%), 57 (95%) and 43 (71.7%) isolates contained SHV-2a, SHV-5 and SHV-12 genes, respectively. Five isolates contained all the three genes of SHV-2a, SHV-5 and SHV-12.

Conclusion: The results indicate the relatively high prevalence of SHV type beta-lactamase genes in K. pneumoniae isolates in Kermanshah. Given this high prevalence, the surveillance of antibiotic resistant patterns and relevant genes is necessary among gram-negative bacilli in Kermanshah region. Due to the high resistance of K. pneumoniae isolates to antibiotics and to reduce the dissemination of resistant genes, susceptibility testing to choose more affective antibiotics is recommended even for isolates from outpatients.

To Study the Association between AcrAB and Qep A Efflux Pumps and Ciprofloxacin Resistance among Escherichia Coli and Klebsiella Pneumoniae Clinical Strains

Mohsen Heidary, Aghil Bahramian, Hossein Goudarzi, Gita Eslami, Ali Hashemi, Saeed Khoshnood,
Volume 19, Issue 4 (7-2016)

Abstract

Background: The efflux pumps are one of the main mechanisms of resistance to antibiotics in E. coli and K. pneumoniae strains. The aim of this study is to study the association between genes coding efflux pumps AcrAB and Qep A and ciprofloxacin resistance among E. coli and K. pneumoniae clinical strains

Materials and Methods: This study was done on 100 strains of E. coli isolated from Taleghani and Labbafinejad Hospitals and 100 strains of K. pneumoniae isolated from Taleghani and Mofid Children Hospitals. Antimicrobial susceptibility tests were performed by disk diffusion method based on CLSI guidelines. Identification of genes encoding efflux pumps Acr AB and Qep A was done by PCR technique.

Results: In this study, fosfomycin and imipenem had the best effect against E. coli clinical isolates and fosfomycin and tigecycline had the best effect against K. pneumoniae clinical isolates. PCR assay demonstrated that the prevalence of AcrA, AcrB and QepA genes among E. coli isolates were 92%, 84% and 0%, respectively and among K. pneumoniae isolates were 94%, 87% and 4%, respectively.

Conclusion: The prevalence of genes encoding efflux pumps in E. coli and K. pneumoniae clinical strains, which causes resistance to fluoroquinolones, is cause for concern. Therefore, controlling infection and preventing the spread of drug-resistant bacteria needs to manage medication carefully and identify resistant isolates.

In vitro Inhibitory Effect of Alcoholic and Aqueousextract of Vaccinium Arctostaphylos on ESBLs Producing Klebsiella Strains Isolated from Clinical Specimens in Karaj

Sare Karimi, Azam Haddadi, Parvin Torabzadeh,
Volume 21, Issue 2 (5-2018)

Abstract

Abstract
Background: In recent years, increasingly antibiotic resistance problem among Klebsiella isolates and side effects of antibiotics overuse have made researchers to study the antimicrobial activity of medicinal plants. The aim of this study was to study the inhibitory effect of ethanolic and aqueous extract of Vaccinium arctostaphylos on ESBLs producing Klebsiella strains.
Materials and Methods: Among 143 isolates of Klebsiella collected from some hospitals and clinical laboratories in Karaj, ESBLs producer were screened by phenotypic confirmatory test (PCT). One of them was identified based on 16S rRNA gene sequencing. MIC and MBC of ethanol and aqueous extracts of Vaccinium arctostaphylos were determined using microdilution method on selected ESBLs producing Klebsiella strains.
Results: Resistance to ceftazidime, ceftriaxon and cefotaxime was observed in 14.7% of the isolates. 32 isolates (22%) were detected as ESBL producers. Results of MIC and MBC tests showed that ethanolic and aqueous extract of Vaccinium arctostaphylos have inhibitory effect on ESBLs producing Klebsiella strains,
Conclusion: The presence of antibacterial activity could be confirmed in most plant species used in traditional medicine in Iran and we should focus on combining traditional medicines and modern drugs.

Integron-associated Antibiotic Resistance in Clinical Isolates of Klebsiella from Karaj

Hiva Saki, Azam Haddadi, Mahmoud Shavandi,
Volume 21, Issue 7 (2-2019)

Abstract

Background and Aim: In recent years, Multidrug resistance has been increasing among Klebsiella isolates. The aim of this study was to survey existence of integrons and its relation with antibiotic resistance among clinical isolates of Klebsiella.
Materials and Methods: From Jun 2015 to May 2016, 129 Klebsiella isolates collected from Karaj hospitals and laboratories. Statistical population included 80.6% female and 19.4% male. Antimicrobial susceptibility was performed using Kirby-Bauer disk diffusion and ESBLs producer were screened. Integrons were detected using PCR.
Ethical Considerations: This study with research ethics code IR.IAU.K.REC.1396.16 has been approved by research ethics committee at Islamic Azad University of Karaj, Iran.
Findings: The highest and lowest percentage of sensivity were found to ofloxacin (89.1%) and amoxcicillin (6.2%), respectively. 82.9% of isolates were resistant to more than two antibiotics from different classes. Among 129 isolates, 19.3% of the isolates harbour integrons. Frequencies of MDR among integron-positive isolates were 100%. Also, 71.3% and 28.7% of isolates were ESBLs positive and negative respectively.
Conclusion: Results showed integron elements were prevalent among MDR isolates. Integron-associated resistance genes can be served as reservoirs of multi drug resistance within clinical isolates and presence of integron can be used as a marker to identify MDR isolates. Prevalence of ESBLs among clinical isolates of Klebsiella showed that antibiotics like ampicillin or amoxicillin-clavulanic acid are not effecvtive anymore in treatment of UTIs.

Investigation of qnrB and qnrS genes frequency in E.Coli and Klebsiella Pneumoniae isolates in children with UTI in Rasht city

Ensiyeh Abbaspour Naderi, Mohammad Ali Bepouei, Mahzad Diar, Matin Mohamadi, Mohammad Hedayati, Mahdi Shahriarinour,
Volume 26, Issue 4 (11-2023)

Abstract

Introduction: Urinary tract infection (UTI) is one of the most important and common infections in children. The aim of this study was to investigate the frequency of qnrB and qnrS genes in Escherichia coli and Klebsiella pneumoniae isolated from urinary tract infections of children in 17 Shahrivar Hospital in Rasht.
Materials and methods: In this descriptive cross-sectional study, 49 strains of Escherichia coli and Klebsiella pneumoniae were isolated from 17 Shahrivar Hospital in Rasht and identified using biochemical methods. Sensitivity and resistance of strains to antibiotics were determined by Kirby Bohr and dilution broth methods. PCR method was used to evaluate the frequency of qnrS and qnrB genes in isolates.
Results: In this study, the highest resistance was observed in piperacillin (81.5%) and cefazolin (88.9%) isolates from Escherichia coli and in Klebsiella pneumoniae (cefazolin (90.9%) and amoxicillin (95.5%) isolates from 49 Isolated, 73.4% had qnrB gene and 97.9% had qnrS gene.
Conclusion: It seems that one of the reasons for increasing multidrug resistance in hospital isolates of urinary tract infection (UTI) in Rasht is the increased transfer of plasmid genes between these isolates.

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