Showing 6 results for Inhibition
Pedrama Ariapanah, Morteza Sattari, Zahra Jafari-Azar, Adonis Poormohammadi Mojaveri,
Volume 13, Issue 4 (1-2011)
Abstract
Background: Due to problems caused by traditional dressings, scientists have long been in search for producing alternative cellulose. Unique characteristics of bacterial cellulose synthesized by acetobacter xylinum, due to its nanostructure cellulose, resulted in attempts to devise an ideal dressing with this cellulose. The main aim of this study is to evaluate the effect of impregnated bacterial cellulose on staphylococcus aureus culture. Materials and Methods: In this descriptive-analytical study, cellulose disks synthesized by bacterial cellulose and cellulose blank disks (without antibiotic) were placed in 3.3% ciprofloxacin hydrochloride. These disks were, then, together with ciprofloxacin standard, control cellulose, and cellulose blank disks, placed on the cultured media of staphylococcus aureus. After 24 hours, the results were obtained through the measurement of growth inhibition zone. Determining the amount of antibiotic absorbed into bacterial cellulose can be done through the comparison of the effects of cellulose disks containing different concentrations of ciprofloxacin hydrochloride and ciprofloxacin standard disks. Results: Both cellulose and blank disks created a growth inhibition zone in staphylococcus aureus media, whereas the growth inhibition zone of cellulose and cellulose blank disks (negative control) were insignificant. Conclusion: Noticing the unique characteristics of bacterial cellulose as a dressing and its proven ability in absorption and release of ciprofloxacin hydrochloride, the prospects are seen for production of antibiotics containing dressings of this microbial product in future.
Farideh Tabatabaei Yazdi, Behrooz Alizade Behbahani, Maryam Heidari Sureshjani,
Volume 17, Issue 3 (6-2014)
Abstract
Background: The overuse of therapeutic antibiotics results in the drug resistance. The excessive use of antibiotics causes the mutations in the microorganisms and the emergence of new microorganisms which are resistant against the common antibiotics. With regard to limitations and known side effects of antibiotics, the exploring of antimicrobial compounds seems necessary. The purpose of this study is to evaluate the antimicrobial effects of aqueous and ethanolic extracts of Chevil against Staphylococcus epidermidis PTCC 1435, Yersinia enterocolitica PTCC 1221 and Enterobacter aeruginosa PTCC 1151 and to compare them with the common therapeutic antibiotics.
Materials and Methods: In this experimental study, after collecting plants from the highlands of Chaharmahal va Bakhtiari province, the extraction was carried out by the maceration method. To evaluate the antimicrobial activity, Disc diffusion test with Kirby-Bauer method was used. The Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentration (MBC) were determined by using the dilution method.
Results: The highest inhibition zone diameter in 40 mg/ml was related to Staphylococcus epidermidis and the minimum diameter in this concentration was related to Gram-negative bacteria, Enterobacter aeruginosa. MIC of aqueous and ethanolic extracts for Enterobacter aeruginosa 64 and 32 mg/ml and MBC of ethanolic and aqueous extracts of Enterobacter aeruginosa were 128 and 64 mg/ml respectively.
Conclusion: The ethanolic extract of the Chevil compared with the common therapeutic antibiotics had more inhibitory effect on studied bacteria. Furthermore, Chevil extracts showed greater inhibitory effect on Gram-positive bacteria in comparison with Gram-negative bacteria.
Hassan Izanloo, Mohammad Ahmadi Jebelli, Shahram Nazari, Navid Safavi, Hamid Reza Tashayoe, Gharib Majidi, Mohammad Khazaei, Vahid Vaziri Rad, Behnam Vakili, Hussein Aghababaei,
Volume 17, Issue 9 (12-2014)
Abstract
Background: This study aimed to examine the antibacterial effect of Polyamidoamine-G4 dendrimer on Escherichia coli, Enterobacter cloacae, Bacillus subtilis and Staphylococcus aureus.
Materials and Methods: In this experimental study, the antibacterial effects of Polyamidoamine-G4 dendrimer were studied by disc diffusion and micro-dilution method. Different concentrations of Polyamidoamine-G4 inoculated onto blank disks and were placed in Mueller-Hinton agar media. Zone of inhibition was investigated by bacterial inoculation according to the McFarland standard 0.5. Minimum inhibitory concentration and minimum bactericidal concentration of Polyamidoamine-G4 dendrimer were determined by micro-dilution method in nutrient broth culture.
Results: Zone of inhibition in concentration 500 &mug/ml of Polyamidoamine-G4 dendrimers for Escherichia coli, Enterobacter cloacae, Bacillus subtilis and Staphylococcus aureus were 14, 0, 35 and 29mm, respectively. Concerning the Zone of inhibition in gram negative bacteria with gram positive ones was p<0.05 and had significant difference. The minimum inhibitory concentration of Polyamidoamine-G4 dendrimer for Escherichia coli, Bacillus subtilis and Staphylococcus aureus were 1250, 2.5, and 1 &mug/ml, respectively. The minimum bactericidal concentration of Polyamidoamine-G4 dendrimer belonged to Escherichia coli, Bacillus subtilis and Staphylococcus aureus were 2500, 5 and 5 &mug/ml, respectively. Polyamidoamine-G4 dendrimer had not bacteriostatic and bactericidal effects on Enterobacter cloacae.
Conclusion: According to the results, Polyamidoamine-G4 dendrimer can eliminate Escherichia coli, Staphylococcus aureus and Bacillus subtilis effectively. It is suggested in the rest of this study that the probable toxicity of nanostructured compounds examined in drinking water and, economic studies is done for synthesis and their applications in case of prevention of using.
Majid Talebi, Dariush Minai-Tehrani, Mohammad Fazilati, Arash Minai-Tehrani,
Volume 20, Issue 9 (12-2017)
Abstract
Abstract
Background: Lipases are one of the groups of enzymes which are important in medicine and industry. In gastrointestinal tract of human, lipase catalysis break down of triglyceride. Bacteria have also lipases which break down triglyceride in their culture medium and can use its fatty acids as carbon source. Dicyclomine is an antispasmodic drug that relieves smooth muscle spasm of the gastrointestinal tract. Few drugs have been determined to inhibit lipase activity, in this research; dicyclomine was introduced as an inhibitor of lipase, for the first time.
Materials and Methods: Lipase was extracted from Pseudomonas aeruginosa and the effect of dicyclomine was tested on its activity. The bacterium was cultured in medium with olive oil as carbon source and the supernatant was used for enzyme assay. For monitoring the enzyme activity, Para nitrophenyl palmitate (pNPP) was used as a substrate and the induced product was measured by visible spectrophotometer. Lineweaver-Burk plot was utilized for revealing the type of binding.
Results: This research has shown that dicyclomine can inhibit lipase. Inhibition plot determined that the drug can inhibit the enzyme by competitive manner. Calculation of Ki and IC50 values showed that the drug can bind to the enzyme with high affinity. The study of enzyme activity in different pH and temperature revealed that optimum activity of enzyme, in the presence or absence of the drug, was observed in pH 8 and 60oC.
Conclusion: For the first time, dicyclomine was introduced as an inhibitor of lipase and the kinetics factors of the drug were investigated.
Ms Kobraa Mastery Farahani, Dr Soghra Akbari Chermahini,
Volume 25, Issue 5 (12-2022)
Abstract
Introduction: Investigating the psychological issues of patients with diabetes, especially in adolescence and youth, is particularly important. Therefore, this study aims to compare the health anxiety and response inhibition of people with type 1 diabetes with healthy people.
Methods: This research is a description of the causal and comparative types. Its statistical population consists of all boys and girls aged 14 to 24 years old with type 1 diabetes in Arak city who were referred to the treatment clinics of this city in 1400. From this population, 30 people were selected by purposive sampling, and 34 healthy teenagers participated in this study as a comparison group. The tools of this research included the health anxiety questionnaire (Salkoskis and Warwick, 2002) and the open-ended task, and multivariate analysis of variance was used to analyze the data.
Ethical Considerations: The present research has been registered with the code IR.ARAKU.REC.1401.015 in the Ethics Committee of Arak University.
Results: The present study's findings showed a significant difference between health anxiety and cognitive inhibition of adolescents with type 1 diabetes and healthy adolescents (P<0.05).
Conclusions: The results of this research show the higher level of health anxiety and poor performance of adolescents with type 1 diabetes in choosing the right answer and neglect the wrong answer, which indicates a weakness in their inhibition system. Considering the placement of these problems along with the psychological characteristics of adolescence, it is suggested to use suitable psychological treatment approaches and suitable cognitive rehabilitation interventions to solve or reduce these problems and increase the health of these patients.
Dr Fereshteh Amouzadeh, Dr Alireza Bahrami, Dr Mehdi Rahimzadeh,
Volume 26, Issue 4 (11-2023)
Abstract
Abstract
Background and aim: Attention deficit hyperactivity disorder is very common in athletes. This study aims to investigate the impact of transcranial electrical stimulation of alternating current on response inhibition and selective attention in athletes with ADHD.
Materials and methods: The present study was quasi-experimental with a pretest, posttest, and follow-up design with the control group. The statistical population was 160 elite athletes with ADHD aged 9 to 11 years (60 girls and 100 boys). Of these, 120 athletes with ADHD were randomly selected and assigned to three groups of 40: control, sham, and tACS. First, a pretest was done, and then all subjects in the sham and experimental groups received F3(Anodal) and F4(Cathodic) fake and real stimulations, respectively, with a current of 1 mA (10 Hz) for 15 minutes in 10 sessions. The control group did not receive any intervention, then each group was re-examined. A follow-up test was done after two weeks. The Continuous Performance Test (CPT) and Stroop were used in this study. For data analysis, Mixed Anova, one-way ANOVA, and Benferroni's post hoc test were used with using Spss version 21 statistical software (p≤0.05).
Ethical considerations: This study is approved sport sciences Reserch Institute with ethical registration code IR.SSRI.REC.1401.1361.Parents gave written informed consent form.
Results: The results showed that tACS was effective in the response inhibition and selective attention of athletes with ADHD and led to the improvement of response inhibition and selective attention of these subjects.
Conclusion: Based on the findings, the tACS protocol of 1 mA can be used as an effective treatment to improve inhibition and selective attention of athletes with ADHD.
