Showing 25 results for Human
Mohammad Ali Shariatzadeh, Ghasem Mosayebi, Naser Mahdavi Shahri , Ehsanollah Ghaznavirad, Ali Fani, Abdolrahman Dezfulian, Ali Chehreei ,
Volume 6, Issue 3 (10-2003)
Abstract
Introduction: Insulin-dependent diabetes mellitus (IDDM) or type 1 diabetes is an organ-specific autoimmune disease that caused by destruction of insulin-producing beta cells of the pancreas. Etiology of this disease is still unknown. It is seen that genetic and environmental factors play an important role for susceptibility to develop type 1 diabetes. The relationship between HLA associated factors and susceptibility to IDDM disease, was reported by several investigators. Also, some studies show that dermatoglyphics is associated with type 1 diabetes. However, it is maybe there is an association between HLA and dermatoglyphics inpatients'with type 1 diabetes and these characteristics could be applied for diagnosis of disease.
Materials and Methods: In this study, the prevalence of HLA (with using standard microlymphocytotoxicity method) and dermatoglyphics determined in 30 Iranian patients with IDDM and 30 normal healthy controls with similar ethnic background and the same geographical area.
Results: A significantly higher frequency of HLA-DQ, A2, DR3 and DQ2 were found in IDDM cases compared to the controls. The results obtain from dermatoglyphics showed that line ab was reduced in male and female type 1 diabetes. The reciever operating chractristics curve showed that the positive point for lines ab in right and left hands were 34.7 and 35.25, respectively.
Discussion: There is no association between HLA and dermatoglyphics.
With considering of genes encoding of HLA separated from genes determining dermatoglyphics, HLA typing and dermatoglyphics seem to be interesting tools for genetic studies related to type 1 diabetes. Further studies are recommended in order to provide more insight into the susceptibility to this disorder.
Mahdi Sharif, Masoud Keyghobadi, Hajar Ziaei, Jamshid Izadi, Shirzad Gholami, Alireza Khaliliyan,
Volume 8, Issue 2 (7-2005)
Abstract
Introduction: Different strains of Echinococcus Granulosus have been found in endemic areas of Iran. This variation has a significant aspect in the field of medical parasitology and veterinary and also in epidemiology, pathology, control and prevention of Hydatid cyst infection. Morphology, epidemiology and biochemical studies can be carried out to determine Hydatid cyst strains in human and animal origins as host. This study was done to measure different biochemical compositions of liver Hydatid cyst fluids in human, sheep, goat, cattle and camel in 2004.
Materials and Methods: In a cross sectional-analytical study, 112 samples of Hydatid fluids were collected from the liver cysts of different hosts: 16 sheeps, 64 cattles, 12 goats and 10 camels in slaughter houses of Sari and Ghaemshahr and 10 human in Imam hospital. All cyst fluids were centrifuged at 4500 rpm at 4˚C for 45 minutes and the supernatants were analyzed for various biochemical parameters.
Results: Quantitative differences were observed in the levels of Sodium, Glucose, Urea, Alanin Aminotransferase (AST) in liver cystic fluids obtained from different hosts, although these differences were not statistically insignificant. However, differences in the levels of Potassium, Calcium, Triglycerides, Cholesterol, Uric acid, Creatinin, Albumin, Gamma Glutamyl Transferase, Aspartat Aminotransferase (AST) and Creatinine Phosphokinase (CPK) in different Hydatid cyst fluids were statistically significant (P<0.05).
Conclusion: Differences in biochemical composition of different Hydatid cyst fluids suggest the possible existence of more than one strain of Echinococcus Granulosus in human and other intermediate domestic animal hosts in Mazandaran.
Dr Ghasem Mosayebi, Dr Ehsanola Ghaznavi Rad, Dr Ali Fani, Dr Seyedmohammad Moazenni,
Volume 8, Issue 3 (1-2006)
Abstract
Insulin dependent diabetes mellitus, human leukocyte antigens
Introduction: Insulin dependent diabetes mellitus (IDDM) or type 1 diabetes is created by autoimmune destruction of insulin-producing beta cells of the pancreas in genetically susceptible individuals. The relationship between human leukocyte antigens (HLA) associated factors and susceptibility to IDDM disease, was reported by several investigators. Association with different HLA types depends also on the studied populations. The aim of the present study was to determine HLA antigens which represent a high susceptibility to develop the IDDM disease in this area. Materials and Methods: In this study, the prevalence of HLA class-I and II antigens has been determined in 31 Arakian patients with IDDM and 57 normal healthy controls with similar ethnic background and from the same geographical area. The typing of HLA antigens was carried out using standard microlymphocytotoxicity method. Results: A significantly higher frequency of HLA-A2, A3, B21, DR3 and DQ2 were found in IDDM cases compared to the controls. In contrast, HLA-DR2, DR7 and B53 were represented at a somewhat higher frequency in controls compared to the IDDM patients. Conclusion: These results indicate that HLA-A2, A3, B21, DR3 and DQ2 antigens contribute to susceptibility to IDDM independently and HLA-DR2, DR7 and B53 antigens maybe associated with prevention of IDDM in Arakian patients.
Mehdi Mosayebi, Ehsan Ghaznavi, Abdolhossein Dalimi, Mohamad Moazeni, Ghasem Mosayebi, Mahmoudreza Khazaii,
Volume 9, Issue 3 (9-2006)
Abstract
Introduction: There is difference between susceptibility or resistance to infectious diseases such as cystic and alveolar Eclinococcosis in human and animals, that is due to the difference between individual host factors and immunologic responses. This study is done to investigate the resistance and susceptibility markers (HLA) in Hydatid patients and healthy persons. Materials and Methods: This analythical (case-control) study is carried out on 60 patients with confirmed cystic echinococcosis and 30 healthy individuals living in Arak. Blood samples were gathered and tested by microlymphocytotoxicity method. At first diagnostic kits with specific antiserusms for each antigen (28 antigens) were provided and then lymphocytes were separated. After dye and stabling with formalin and based on cells morphology, results were seen by invert microscope. Data was analyzed using Odds Ratio, Relative Risk, Preventive Fraction, Aetiologic Fraction and Chi square test. Results: Results showed that HLA-A1 was significantly higher in patients (p<0.05), and people having this antigen are more susceptible for the infection. In spite, HLA-A10 was higher is healthy individuals (p<0.05) and have a preventive effect in disease involvement. Other investigated antigens had no signigicant difference in the two groups. For more accurate results molecular investigation is needed. Conclusion: In individuals having HLA-A1 there is more chance for cyst growth in confronting hydatosis and this individuals are more susceptible to the disease. But in individuals having HLA- A10 there is less chance for cyst growth in confronting hydatosis and this antigen have a preventive effect against hydatid cyst.
Rahmat Allah Jadidi, Afsaneh Noroozi, Esmaeil Moshri,
Volume 12, Issue 2 (9-2009)
Abstract
Background: Recognition of mortality-related factors in Intensive Care Units (ICUs) could increase efficiency and effectiveness of these units. This research determined the relationship between the physical structure, human resources and their relevance to mortality rate in Intensive Care Units. Methods and Materials: In this cross-sectional study, all ICUs of hospitals in Markazi province were considered. Data gathering tools were resource (human, physical and training) questionnaires and checklists which their reliability and validity were accepted by Monitoring and Validation Center of Ministry of Health. Mortality rate after 24 hours of admition was also determined in relation to hospital resources and activities. Data were analyzed with descriptive statistics, Spearman and Kruskal-Wallis tests. Results: Total score of human, physical and training resources in hospitals of Markazi Province is 55 percent of the country standard score. Mortality ratio after 24 hours of admission, to all patients (released and deceased patients) was highest in Vali-e-Asr hospital (25%) and lowest in Saveh Shahid Chamran Hospital (5%). There wasn’t significant difference in this ratio among hospitals. There were no significant difference between obtained scores of each resource (human, physical and training) among University, Social Insurance and Qods (private) hospitals with respect to mortality rate 24 hours of admission. Conclusion: Human, physical and training resources have not direct effect on decreasing mortality rate after 24 hours of admission but can create necessary conditions for providing higher quality services.
Hadi Ansarihadipour, Hasan Ziafatikafi,
Volume 14, Issue 6 (1-2012)
Abstract
Background: Biochemical studies have shown that iron produces reactive oxygen species via Haber-Weiss and Fenton reactions. The goal of this study is to examine the role of iron in oxidation of human hemoglobin and its structural changes in erythrocytes.
Materials and Methods: In this experimental study, blood samples from healthy subjects were incubated aerobically with the iron containing metal catalyzed oxidation (MCO) system in the presence of 0.036, 0.7, 0.14, 0.28, 0.57, 1.14, 2.28, 4.55, 9.09, and 18.18 micromole of iron. Structural changes in Hb were followed by spectrophotometric analysis from 300 to 650 nm. In addition, carbonyl assay was performed for estimation of protein oxidation in globin chains.
Results: Based on the results, oxy-Hb decreased up to 68% in iron-treated erythrocytes. Decrease in the absorbance ratio (A577, A542 wavelength) indicated the conversion of oxy-Hb to met-Hb. Also, met-Hb concentration was 4.7 fold of hemichrome. After 24 hours of incubation, oxyHb concentration decreased up to 50% and metHb concentration increased up to 85%. Moreover, increase in iron concentration resulted in significant carbonyl formation in hemoglobin.
Conclusion: These findings indicate that Hb oxidation instead of its oxygenation leads to anemia and hypoxia. The findings of this study may be directly applicable to oxidation states during hemolytic diseases and iron treatment.
Mahdi Paryan, Samira Mohammadi Yeganeh, Behzad Khansari Nejad, Mahdieh Mondanizadeh, Saeed Paryan,
Volume 15, Issue 4 (9-2012)
Abstract
Background: Several different molecular methods have been developed that are capable of detecting HIV-1 in clinical specimens with different levels of sensitivity and specificity. This article describes the results of a reliability study on the development and application of a new real-time TMA method for isothermal detection of HIV-1. Materials and Methods: In this ex Primental study, the molecular beacon primer and probe set were designed for a 176-base-pair region of HIV-1 pol gene using a specialized software. Logarithmic serial dilutions from 10-107 copies of an in-vitro transcribed RNA were used for determination of the analytical sensitivity of the assay. Clinical specimens that had previously been evaluated positive or negative by a valid commercial assay were used for assessing the clinical sensitivity and specificity of the assay. Results: The analytical and clinical sensitivities of the assay were determined 500 copies/ml and 93.3%, respectively. The primers and the probe were HIV-1 specific and no cross-reaction was observed with other blood-borne viruses and human genome bioinformatically. The clinical specificity of the developed real-time TMA assay was examined experimentally using 20 negative samples and determined to be 100%. Conclusion: The developed real-time TMA assay can be used as an appropriate tool for the rapid and isothermal detection of HIV-1 in patients' blood and plasma samples.
Hadi Ansarihadipour, Ali Bahadori Vatankhah, Saeed Ziraki, Mohamad Saiadi,
Volume 15, Issue 8 (1-2013)
Abstract
Background: The presence of oxidant agents yields higher levels of free radical reaction products in erythrocyte membrane proteins and serum proteins. The aim of this study is to investigate the oxidative modifications of recombinant human coagulation factor VIII (rHFVIII) by spectrophotometric analysis. Materials and Methods: In this experimental study, rHFVIII was incubated aerobically with vitamin C and ferro ions in metal catalyzed oxidation (MCO) system for 4 to 28 hr. Carbonyl assay was used as an index of protein oxidation. For this purpose, 2,4dinitrophenyl hydrazine (DNPH) was used. Reaction of this reagent with carbonyl groups produces dinitrophenylhydrazone derivatives that their concentration was estimated by spectrophotometry. Results: Carbonyl groups in rHFVIII changed in the presence of vitamin C and ferro ions. Dose-dependent effects of vitamin C showed a decrease in carbonyl groups of rHFVIII whileferro ions increased oxidation and carbonyl group formation in this protein. Conclusion: These findings indicate that changes in carbonyl groups in rHFVIII are related to the generation of reactive oxygen species. Also, antioxidant mechanisms are activated in a dose- and time-dependent manner.
Mana Shojapour, Ghasem Mosayebi, Fardin Faraji, Keyvan Faraji, Ali Ghazavi,
Volume 17, Issue 3 (6-2014)
Abstract
Background: Multiple sclerosis (MS) is an autoimmune disorder with unknown etiology. Genetic and environmental factors associated with MS susceptibility. Genetic studies show an important role for human leukocyte antigens (HLA) and susceptibility to autoimmune diseases such as MS. There is controversy between the association of HLA alleles with MS susceptibility in various studies. However, with consider the high incidence of MS in Iranian population and limit information about association of HLA and MS, we analyzed HLA alleles in MS patients.
Materials and Methods: In this case-control study, 60 MS patients and 40 normal individuals with the same ethic background and geographic area were analyzed for HLA-DRB and DQB alleles by single specific primer-polymerase chain reaction (SSP-PCR) method.
Results: HLA-DRB1*03 and DQB1*02 alleles frequencies in MS patients were greater than healthy controls. There was no significant difference in frequency of other HLA-DR alleles between the MS patients and normal individuals.
Conclusion: DRB1*03 and DQB1*02 alleles confer increased susceptibility to MS in this population. However, to determine the role of HLA in Iranian MS patients, more studies are needed.
Behzad Khansarinejad, Mahdieh Mondanizadeh, Mohammad Rafeie, Siamak Mirab Samiee,
Volume 17, Issue 4 (7-2014)
Abstract
Background: The Real-time PCR assay has been established as the standard method for Human Cytomegalovirus (HCMV) quantitation in immunocompromised patients. However, the question of which one of whole blood or plasma specimens is better for viral quantitation is still unresolved for many clinical laboratories. To answer this question, the current study compares HCMV DNA load in whole blood and plasma samples.
Materials and Methods: In this prospective study, the whole blood and plasma samples were obtained from 41 transplantated patients and the viral load was detected using a validated, in-house Real-time PCR assay.
Results: Of the total 193 examined specimens, 174 were negative and 19 samples, from 16 patients, were positive in at least one of whole blood or plasma samples. Based on the results of linear regression analysis, the cytomegalovirus viral load was correlated in whole blood and plasma samples (R2: 0.872). However, the regression equation shows that the HCMV load in whole blood samples is higher than load of this virus in plasma. The validity of the quantitative results was confirmed by repeating the tests and analyzing the results using the repeated measure analysis.
Conclusion: Based on the results of the present study, HCMV quantitation in whole blood samples has a higher analytical sensitivity than in plasma samples.
Mohammad Reza Dayer, Nooshin Azari, Nematollah Razmi, Mohammad Saeid Dayer,
Volume 17, Issue 7 (10-2014)
Abstract
Background: Prion diseases are neurodegenerative disorders which ultimately results in the death of their victims. They are caused by structural transformation of cellular prion (PrPC) to its &beta-rich and anomalous isoform (PrPSc) and the accumulation of amyloid fibrillar deposits in the central nervous system. The precise mechanism underling this conversion is yet to be well understood. This study aimed to investigate the effect of non physiological temperatures on the misfolding mechanism of the human prion protein.
Materials and Methods: The crystal structure of human prion protein (90-231), (PDB code: 2Lej) in pdb format was used as a starting structure in this study. Three model structures of this coordinate structure were used separately to simulate PrPC at 27 , 37 and 47 . Molecular dynamic simulations were then performed using double-precision MPI version of GROMACS 4.5.5 for 10 ns and the results were analyzed using SPSS software, SPDBV and VebLab programs.
Results: The change of temperature from 37 to 27 or 47 induced significant structural changes to PrPC. These tempratures caused PrPC to attain a more folded and less flexible tertiary structure compared to its native structure at 37 . They, also, reduce protein-solvent hydrogen bonds and therefore increasing access of hydrophobic solvent to PrPC which may be behind the lower water solubility of PrPC and its increased resistance to proteolytic degradations.
Conclusion: This study shows that changes of temperatures accelerate structural changes of PrPC and reduce its solubility while rendering it vulnerable to transition into PrPSc.
Roohollah Dorostkar, Mohammad Sadegh Hashemzadeh, Mahdi Tat, Mohammad Raza Shafaati, Mohammad Najarasl, Samaneh Zahiri Yeganeh,
Volume 18, Issue 3 (6-2015)
Abstract
Background: In 1970, human papillomavirus (HPV) was introduced as the main etiologic factor of cervical carcinoma. Since there is no possibility of detecting the virus and its subtypes using serological methods and cell culture, the molecular methods such as PCR have particular importance in accurate, early and definite diagnosis of the virus. So, in this research, our goal is to use a proprietary PCR assay based on L1 gene of human papillomavirus for molecular recognition of HPV and to evaluate its prevalence in patient samples.
Materials and Methods: In this experimental study, after collecting of samples from malignant cervical lesions, the viral DNA was extracted from paraffin blocks of 50 clinical samples and PCR was done by specific primers for L1 gene of human papillomavirus in all samples. After the analysis of PCR products by 2% agarose gel electrophoresis, sensitivity and specificity of the test were also evaluated.
Results: Among 50 patient samples, 33 cases were confirmed to be positive for HPV infection and 17 cases were negative, showing high frequency of HPV in this patient population (about 66%). The results of specificity assay were positive for papilloma samples and sensitivity of the assay was 20 copies of recombinant construct containing L1 per reaction.
Conclusion: This study showed that PCR by specific primers for L1 gene of human papilloma virus is a proper and accurate method for detection of this virus and the results confirm the previous reports of correlation between HPV and cervical carcinoma.
Shahrzad Hadichegeni, Bahram Goliaei, Mehrdad Hsahemi,
Volume 18, Issue 7 (10-2015)
Abstract
Background: Human serum albumin (HSA) is a soluble blood protein which can bind to small molecules (such as drugs and toxins) and transfer them within the blood circulation.
Materials and Methods: UV-Vis spectroscopy and FT-IR methods were used to characterize the binding properties of HSA with diazinon(the toxin of organophosphate) and to investigate the changes of protein secondary structure, respectively, in molecular level under physiological condition in two times of first and thirty five days .
Results: The binding constant (KDiazinon-HSA = 3.367 ) was have been calculated based UV-Vis spectroscopy data. In FT-IR method, the proportion of decrease in percentage of &alpha-Helix on the first day was 53.97% to 51.88%, other secondary structures increased, such as Turns from 8.49% to10.21%, ß-Sheet from 13.94% to 14.81%, &beta-anti from 8.2% to %8.25 and r-coils from 15.4% to % 17.24. These changes for &alpha-Helixes, Turns, ß-Sheet, &beta- anti and random r-coils after thirty five days were 56.7% to 47.11%, 25.3% to 29.75%, 6.93% to 10.94%, 2% to 2.83% and 9.08% to 10.86%, respectively.
Conclusion: Since the content of protein secondary structure relates closely with its biological activity, therefore, a decrease in &alpha-helix and increase in &beta-sheet structure in the presence of diazinon at high concentration means the decrease of HSA biological activity. Our results suggest that diazinon has a relatively good binding with HSA and it could cause considerable changes in various secondary structures and likely is indicative of a unfolding of protein especially for the samples in thirty five days .
Seyed Reza Mohebbi, Hamed Naghoosi, Pedram Azimzadeh, Shaghayegh Derakhshani, Afsaneh Sharifian, Mohammad Reza Zali,
Volume 18, Issue 7 (10-2015)
Abstract
Background: In spite of designing and applying an effective vaccine against Hepatitis B virus (HBV), chronic infection with this virus is still one of the most important health problems worldwide. Host genetic background including single nucleotide polymorphisms play a significant role in chronicity or clearance of the infection. The final product of programmed cell death 1 gene (PDCD1) is expressed frequently on T-cells and in chronic viral infections, prevent the virus-specific T-cell response against the virus. In this study, the association of a single nucleotide polymorphism (+7146A/G) in intron 4 of PD1 gene with chronic hepatitis B infection in Iranian population has been assessed.
Materials and Methods: 212 chronic HBV patients and 208 healthy controls were analyzed in this case-control study. Genomic DNA of the studied individuals was extracted and after performing polymerase chain reaction (PCR), polymorphism of +7146 was determined via RFLP method.
Results: Frequencies of GG, GA and AA genotypes on position 7146 of the intron 4 of PD1 gene were 77.4%, 20.7% and 1.9% in patient group and 80.8%, 15.4% and 3.8% in control group, respectively. After statistical analysis, No significant difference was observed between patient and control groups (p=0.198).
Conclusion: Genotype frequencies in the studied population are in accordance with the results of previous studies. Results of the present study suggest that there is not any association between A/G single nucleotide polymorphism in intron 4 of PD1 gene and susceptibility to chronic hepatitis B in Iranian population.
Homa Dadgarnia, Zahra Hajebrahimi,
Volume 19, Issue 2 (5-2016)
Abstract
Background: Endothelial cells are very sensitive to mechanical force including microgravity and the morphological and functional changes in them are believed to be at the basis of weightlessness-induced cardiovascular deconditioning. It has been shown that the proliferation, migration, and morphological differentiation of endothelial cells play critical roles in angiogenesis. So far, the influence of microgravity on the ability of endothelial cells to foster angiogenesis remains to be explored in detail. The aim of this study was to investigate the effect of microgravity condition on VEGFR-2 and CD34 genes expression in human umbilical vein endothelial cells (HUVEC) in angiogenesis.
Materials and Methods: In this study, HUVEC cells were purchased from Pastor Institute. We used a clinostat to simulate microgravity condition for 2, 24 and 72 hours. Real time PCR technique was used for gene expression analysis after extraction of RNA from cells.
Results: Our results showed that microgravity for 72h leads to a significant increase (6 times compared with control group, p<0.001) in the VEGFR-2 gene expression. However, expression of CD34 did not change (p>0.05) with microgravity.
Conclusion: Based on the results, microgravity has positive effect on angiogenesis and can be used to generate vascules for cell therapy of ischemic diseases and atherosclerosis.
Fatemeh Ghatreh Samani, Ali Nourian, Laya Farzadi,
Volume 20, Issue 10 (1-2018)
Abstract
Abstract
Background: Ultrasonography (US) is the most important imaging modality in detecting both intrauterine and ectopic pregnancies. This study aimed to compare serum β-hCG level with Transvaginal Ultrasound in symptomatic patients with ectopic pregnancy.
Materials and Methods: A total of 210 patients with definite diagnosis of ectopic pregnancy were enrolled prospectively. All patients underwent transvaginal US by an experienced radiologist who was blind to the final status of the patient. Serum levels of β-hCG and the time gap between US examination and last menstrual period (LMP) were compared between patients with positive and negative US findings.
Results: US were positive in 194 patients (92.4%) and negative in the remaining 16 patients (7.6%). The two groups were similar in terms of age (median, 31 years vs. 30.5 years, respectively; p=0.57). Both the median time gap between US and LMP (median, 43 days vs. 34 days, p=0.03) and serum level of β-hCG (median, 1027 mIU/ml vs. 172.5 mIU/ml, p<0.001) were significantly higher in patients with positive US findings. Both differences were independent of each other in multivariate analysis. The best discriminative zone was set at a serum β-hCG level of 326 mIU/ml with a sensitivity and specificity of 86.6% and 87.5%, respectively.
Conclusion: According to our findings, both the median serum level of β-hCG and time gap between US and LMP in patients with missed diagnosis of ectopic pregnancy were significantly lower in comparison with correct diagnosis and proposed discriminative zone for serum level of β-hCG in our study is different from the previous studies though further studies are recommended especially in symptomatic patients with suspected ectopic pregnancy.
Iman Jamhiri, Saber Zahri, Davood Mehrabani, Zahra Khodabandeh, Ramin Yaghobi, Seyed Younes Hosseini,
Volume 20, Issue 11 (2-2018)
Abstract
Abstract
Background: High morbidity and limited therapies of hepatic fibro genesis are important factor for better understanding the molecular mechanisms of the disease. Advances in the understanding of the molecular behavior of hepatic stellate cells (HSC) allow the progress of a field dedicated to anti-fibrotic therapy. Melanoma differentiation associated gene-7 (IL-24/mda-7) as a gene induced during terminal differentiation in human melanoma cells, but the inflammatory response of cells to IL-24/mda-7 is not entirely cleared.
Materias and Methods: LX-2 cells (a human hepatic stellate cell) were treated by leptin (positive control), media (control negative), or were transfected by empty plasmid and pcDNA3.1/mda-7. The inflammatory state was evaluated through measuring the mRNA expression level of inflammatory molecule, IL-1β. The role of IL-24/mda-7 modulation on inflammatory response was assayed using SOCS1 and SOCS3 gene expressions.
Results: The expression levels of IL-1β, SOCS1 and SOCS3 were compared in LX-2 cell line groups. The expression of the IL-1β in the transfected cells was higher than the control cell, but it was not significant. The results indicated that the expressions of SOCS1 and SOCS3 were up-regulated following pcDNA 3.1/mda-7 transfection into LX-2 cells compared to control plasmids (p=0.0179, p=0.0428).
Conclusion: The endogenous IL-24/mda-7 exhibited a significant modulatory effect on stellate cells. Therefore, IL-24/mda-7 and relevant signaling pathways could be employed as a target for fibrosis treatment.
Sara Karimi Moghadam, Roohollah Dorostkar, Saeed Hesami Takallou,
Volume 20, Issue 11 (2-2018)
Abstract
Abstract
Background: Breast cancer is the most common cancer in Iran and breast cancer is the fifth leading cause of death among women. Diagnosis of breast cancer in early stages could increase the lifetime of more than 90% of patients. Human endogenous retroviruses are as heterochromatic parts of the genome, lack any expression. But in several categories of human cancers, including breast cancer, there is a significant increase in the level of HERV-Kenv mRNA.
Materials and Methods: In this case-control study, blood samples were collected from 40 breast cancer patients admitted in Baqiyatallah Hospital and 20 healthy individuals to study the increased expression of HERV-Kenv mRNA using specific primers and were tested by RT-PCR.
Results: Investigations on the patient and control groups showed that increased expression of mRNA was positive in 60% of patients with breast cancer and negative in all healthy subjects.
Conclusion: The results of this study showed that expression of mRNA HERV Kenv in breast cancer was increased. Since enhancement of mRNA HERV-Kenv in the blood of breast cancer patients occurs in of disease, these retroelements could be used as a diagnostic biomarker
Mahdi Bayati, Reza Gharakhanlou, Maryam Nikkhah, Sadegh Amani Shalamzari,
Volume 21, Issue 3 (6-2018)
Abstract
Background and Aim: Low-volume, high-intensity interval training (HIT) increase skeletal muscle aerobic capacity, yet little is known about the potential mechanisms in improvement of this adaptability. The purpose of present study was to examine the effect of four weeks of HIT on peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) and vascular endothelial growth factor (VEGF) protein contents in skeletal muscle of active men.
Materials and Methods: Eight active male students voluntarily and purposefully participated in this study. One week before the experiment started; subjects were familiar with protocol of research. Needle biopsy samples vastus lateralis were obtained 48 h before training and 72 h after the final training session. HIT protocol consisted of 11-15 bouts of 1 min cycling at ∼85-90% of reserve heart rate separated by 1 min of active recovery between each, 3 sessions per week for 4 weeks. Variables were measured by ELISA. All data were analyzed using paired t-test and at the level of significance of p ≤ 0.05.
Findings: Results of study showed the four weeks of HIT lead to significant increase in PGC-1α and VEGF (p < 0.001).
Conclusion: Our findings suggest that activation of VEGF from PGC-1α pathway is part of cellular-molecular mechanisms of high-intensity interval training. So, probably angiogenesis in skeletal muscle is one of the most important factors in improving of aerobic performance, which requires more studies. |
Komeil Amini, Kamran Mansouri,
Volume 21, Issue 5 (10-2018)
Abstract
Background and Aim: Human papillomavirus (HPV) infection is a prevalent, life-threatening disease and cause of cancer among women. Therefore, in recent years, developing novel anti-HPV agents is highly regarded. The study was planned to bioinformatic screening for E1 and E2 potential inhibitors of HPV serotypes including 16,18,31,33 and 45 types from medicinal plants.
Materials and Methods: This is a descriptive-analytic study. In the first step, three-dimension structure of phytochemicals were retrieved from PubChem database and then the cell cytotoxicity and mutagenesis potential of them were evaluated. In the next step, the amino acid sequences of two key proteins of mentioned types of HPV including E1 and E2 were obtained from Uniprot database. Furthermore, the conserved and variable regions of the protein sequences were predicted using multiple sequence alignment method. Finally, the three-dimension structure of mentioned proteins was determined by homology modeling method and potential interactions of the phytochemicals with the proteins were investigated using molecular docking method through Autodock 4.2.6 software.
Findings: The results demonstrated that ursolic acid has no cytotoxicity and mutagenesis potential with appropriate physicochemical properties. Results also showed that mentioned compound had strong interaction with both E1 and E2 of all studied serotypes. Furthermore, the evaluation of ursolic acid and E1 and E2 interactions showed that amino acid is involved in conserved regions of mentioned serotypes.
Conclusion: Based on the obtained results of present study ursolic acid could be good candidate for more in vitro and in vivo studies of its anti HPV activity.
