---

Background: Dental plaque is composed of bacterial derived extracellular polysacharide known as glucan which is synthesized by Streptococcus mutans. Natural substances that could inhibit the plaque formation of the bacteria have a significant importance. This investigation has evaluated the honey beeswax extract effect on the Gft production, the key enzyme of S. mutans colonization factor for the first time. Methods and Materials: In this experimental study extraction of the sample conducted with ethyl acetate and methanol solutions in the Clevenger extractor. The ethyl acetate soluble fraction was separated in the first step and after the evaporation of the first solute, the 70% methanol as inactive solvent was added and the water mixture was used as a second solution, then materials were separated with dH2O. Minimum inhibitory concentration (MIC) of the honey beeswax extract was assessed by Broth diffusion method. Examination of cell adherence (Biofilm Inhibitory Concentration, BIC) was calculated by colony counts from surface scratching of glass slides in the bacterial media that supplied with 1% sucrose. Glucosyltransferase expression was detected by 15% SDS poly acrylamide gel electrophoresis. Results: Concentration of 1mg/ml of ethyl acetate honey beeswax extract was inhibited completely biofilm and it was prevented the production of glucosyltransferase enzyme. The concentration of formation 6 mg/ml of the extract had bacteriostatic effect and 30 mg/ml concentration of this extract had bactericidal for S. mutans (P<0.01). Conclusion: The sub- bacterial concentrotion honey beeswax extract was able to block the major enzyme that contributes to S. mutans biofilm formation.

---

In the Holy Quran, it is stated that “eating honey has healing for people” “Fihe shefaho lenas”. The word “healing” in Quran has not been used about any other food but honey and one Sura in Quran is dedicated to Nahl (bee). This study investigates the importance of honey from the perspective of Quran and medicine. This review article investigated the therapeutic properties of honey from the perspective of Quran and medicine through text analysis and manual and online search. Medicine has proven that honey due to its low moisture, high osmosis, and high acidity inhibits the growth of infectious agents and creates a sterile coat on the wound. Prophet Mohammad said, “Lam yastashfeo almariz bemasale sharbato olasal”, meaning that nothing cures the sick person the way honey does. Studies have shown that honey is effective in strengthening child development and treatment, prevention, and improvement of skin, respiratory, liver, gallbladder, eyes, ears, and nose diseases and cancer. The application of natural honey to healing diabetic and cutaneous ulcers and the topical application of honey to open wounds can accelerate the healing process. Oral administration of dates honey syrup can enhance the natural progression of delivery and womb contractions which decrease the need for cesarean. It can be concluded from the review of the literature that emphasis of Quran on the use of honey in diet and its reference to the healing property of honey for people has not been without a cause. Modern medicine also verifies the curative properties of honey for many diseases.