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Introduction: There is little information about essence and combination of ductuli efferentes secretions. Glycoconjugates importance in sperm production and maturation has been confirmed in previous studies therefore this study is done in order to recognitize Glycoconjugates in ductuli efferentes epithelium and determination of their distribution pattern by means of lectin histochemistry. Materials and Methods: In this descriptive study tissue species were obtained from 30 adult male BALB/c mice. After fixation and routine laboratory processes, 5 μm sections were prepared from paraffin blocks. These slides were exposed to different lectins by means of lectin histochemistry. Reaction intensity in different cells was investigated by light microscope and graded according to the previous related studies. Then results were compared with each other by Kruskal-Wallis and Dunn statistical tests. Results: The mean of reaction intensity in ductuli efferentes epithelium in reply to different lectins, showed significant statistical difference (p<0.005). The most intense reaction was seen in response to WGA (Wheat Germ Agglutinin) lectin and after that to SBA (Soybean Agglutinin), VVA (Vicia Villosa Agglutinin) and PNA (Peanut Agglutinin) respectively. But the staining was negative with GSA-I (Griffonia Simplicifolia Agglutinin-I) lectin. Conclusion: Results indicate that the cells of ductuli efferentes epithelium in mouse are involved in synthesis and secretion of sperm maturation related glycoconjugates and produce a variety of these components in various amounts. There is a large amount of components, containing terminal sugars such as Sialic acid,Galactose-N-Acetylgalactosamine and N-Acetylgalactosamine in ductuli efferentes cells. The lack of Galactose in this epithelium shows that, it hasn’t a role in sperm maturation.

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Background: Glycoconjugates molecules showed a programmed changes during the development. These components in the cell-surface and extracellular matrix plays important roles for different developmental processes. In this study lectin histochemistry technique was used for identification and localization of some glycoconjugates during the development of thymus. Methods and Materials: Balb/C mice embryos from days 10 to 15 and also day 18 fetuses fixed in formalin and provided 5m serial sections of these samples for histochemical study. Slices were incubated with three types of HRP-conjugated lectins (HRP) include: SBA specified for α, β-D-N-acetylgalactoseamin (α,β-D-GalNAc), PNA specified for β-D-Galactose -(1-3)-D-N-acetylgalactoseamin (β-D-Gal-(1-3)-D-GalNAc) , MPA specified for D-Galactose (D- Gal). Results: SBA lectin was presented with decreasing intensity in the Golgi zone (GZ) and cell surfaces of mesenchymal cells, epithelial cells and T-cells microenvironment in studying days. In first day (12&13) T-cells had high reaction with SBA in membranes and GZ. PNA lectin was revealed with several intensity in basal membranes and membranes of mesenchymal cells, epithelial and microenvironment of T-cells in studying days. MPA lectin weakly presented in the endodermal and mesenchymal cells and microenvironment of T-cells in all days. Conclusion: Results indicated that glycoconjugates molecules with terminal carbohydrate of GalNAc might have a role in the development of thymus gland and maturation of the T-cells. Glycoconjugates with terminal disaccharide of Gal/GalNAc probably have significant effect on the total developmental process of thymus gland. Further, Glycoconjugates with terminal carbohydrate of Gal probably had not a key role in the development of thymus gland and T-cells or with low quantity effect.