---

Background: Albizzia lebbeck is used for the treatment of diarrhea and dysentery. In this study the efficacy of hydro-ethanolic extract of this plant on enteric gram-negative and aerobic gram-positive bacillis was determined. Materials and Methods: In this experimental-clinical trial, cold maceration method by ethanol was used for extraction of Albizzia lebbeck fruit, seed, flower and leaves. Then its polyphenolic components were separated by ethyl acetate. After concentrating the different extracts through disk diffusion and tube dilution, the effect of minimal inhibitory concentration (MIC) and minimal bacteriocidal concentration (MBC) of their antibacterial component on shigella dysenteriae, proteus mirabilis, escherchia coli, bacillus cereus and bacillus anthracis were evaluated. Also, the antibacterial effects of Albizzia lebbeck extracts together and in combination with some antibiotics, were compared through variance analysis. Results: Different extracts of Albizzia lebbeck just had similar and positive effects on shigella dysenteriae, bacillus cereus, and bacillus anthracis. However, the hydro-ethanolic fruit extract was more effective than the falavnoid fruit, flower and leave extracts. MIC and MBC results for antibacterial material on the three aforementioned bacterias were 0.125 and 0.25 gram/ml, respectively while the effects of the polyphenolic components of this plant's fruit were similar to its hydro-ethanolic extracts. Conclusion: Through the identification of different components of Albizzia lebbeck and the attainment of new formulations of these components, the grounds will be provided for its application on treatment of patients with inflammatory diseases and asthma, and on improvement of their immune system.

---

Background: Lamiaceae family has about 200 genuses and 4000 species. The majority of species of the family have essence that was used for nutritional, cosmetic and pharmaceutical industry. The aim of this study was to investigate the chemical composition of essential oil from aerial parts of Bollota platyloma  and antibacterial activity of it.

Materials and Methods: In this experimental study, study Ballota platyloma, belonging to lamiaceae family and entomic of iran, was collected from Versk region in Mazandaran provinc. Essential oil from aerial parts was obtained by hydro distillation method by using Clevenger apparatus. GC/MS analysis of essential oil was carried out to identify major volatile constituents. Extraction was prepared by maceration method. Antibacterial activity of methanolic extracts against Pseudomonas aeruginosa, Bacillus subtilis, Stapylococcus aureus, Escherichia coli was investigated by disc diffusion method and minimal inhibitory concentration(MIC) method.

Results: Results showed that GC/MS analysis of the essential oil confirms the presence of 24 constituents in Ballota platyloma. The main constituents were Hexadecanoic Acid (40.03%), Germacrene D (26.6%) and Beta Caryophylene (4.76%). The results indicated that methanolic extract of Ballota platyloma possessed antibacterial activity. Among the aforementioned bacteria, the highest antibacterial activity was seen against S. aureus and the lowest activity against P. aeruginosa.

Conclusion: The findings of the present study showed that the major component of oil essential was germacrene-D and methanolic extract from the aerial parts of Ballota platyloma Rech. f. possess anti-bacterial effects. Thus, in order to find the underlying mechanism of this activity, further research should be carried out.

---