Showing 5 results for Erythrocyte
Javad Baharara, Farhang Hadad, Alireza Ashraf, Elham Khanderoo,
Volume 11, Issue 2 (6-2008)
Abstract
Introduction: The increasing use of the electromagnetic field producer sets in daily living causes concerns about these waves on human health. The effects of extremely low frequency electromagnetic field (50 Hz) on induction of chromosomal damages on bone marrow erythrocytes of male Balb/C mouse has been investigated in this research. Materials and Methods: This is an experimental study in which the laboratory system of producing electromagnetic with low frequency (50 Hz) was used. Five week old male Balb/C mice were divided into three controls, sham-exposed and experimental groups. The experimental mice exposed were exposed to electromagnetic field (50 gauss) for 4 days (12 hours/day). After treatment, the chromosomal damages were assessed using micronucleus test in polychromatic erythrocytes and resultant quantity data were analyzed using t and Mann-Whitney test. Results: Results showed that frequency of micronucleated polychromatic erythrocytes of experimental group (14/35±1/589) was more than Sham-exposed (8/958±1/049) and control group (7/65±0/768) significantly (p<0/05). Conclusion: Extremely low frequency electromagnetic field (50 Hz) causes chromosomal damages induction in polychromatic erythrocytes of bone marrow male Balb/C mouse.
Hadi Ansarihadipour, Hasan Ziafatikafi,
Volume 14, Issue 6 (1-2012)
Abstract
Background: Biochemical studies have shown that iron produces reactive oxygen species via Haber-Weiss and Fenton reactions. The goal of this study is to examine the role of iron in oxidation of human hemoglobin and its structural changes in erythrocytes.
Materials and Methods: In this experimental study, blood samples from healthy subjects were incubated aerobically with the iron containing metal catalyzed oxidation (MCO) system in the presence of 0.036, 0.7, 0.14, 0.28, 0.57, 1.14, 2.28, 4.55, 9.09, and 18.18 micromole of iron. Structural changes in Hb were followed by spectrophotometric analysis from 300 to 650 nm. In addition, carbonyl assay was performed for estimation of protein oxidation in globin chains.
Results: Based on the results, oxy-Hb decreased up to 68% in iron-treated erythrocytes. Decrease in the absorbance ratio (A577, A542 wavelength) indicated the conversion of oxy-Hb to met-Hb. Also, met-Hb concentration was 4.7 fold of hemichrome. After 24 hours of incubation, oxyHb concentration decreased up to 50% and metHb concentration increased up to 85%. Moreover, increase in iron concentration resulted in significant carbonyl formation in hemoglobin.
Conclusion: These findings indicate that Hb oxidation instead of its oxygenation leads to anemia and hypoxia. The findings of this study may be directly applicable to oxidation states during hemolytic diseases and iron treatment.
Savad Baharara, Zahra Zahedifar, Farhang Haddad, Naser Mahdavi Shari,
Volume 15, Issue 1 (4-2012)
Abstract
Background: Green tea due to its antioxidant properties can prevent some types of chromosomal damage. In this study, the effects of green tea on repressing chromosomal aberrations induced by cell phone waves in bone marrow polychromatic erythrocytes of male Balb/C mice were investigated.
Materials and Methods: In this experimental study, 40 adult male Balb/C mice were randomly divided into five groups: control (natural conditions), sham exposed (no exposure to cell phone waves in vitro), experimental group 1 (exposure to cell phone waves), and experimental group 2 (intraperitoneal injection of 100 mg/kg dose of green tea extract for 5 days and exposure to cell phone waves), and experimental group 3 (intraperitoneal injection of 200 mg/kg dose of green tea extract for 5 days and exposure to cell phone waves). Micronucleus test was run in all groups. Data were analyzed by ANOVA and Tukey tests using SPSS software (p<0.05).
Results: The mean number of micronucleus in polychromatic erythrocytes of the sham group (3.47± 0.253) compared with control group (3.43 ± 0.605) did not show a significant difference (p> 0.05), whereas the mean micronucleus frequency in the experimental group 1 (5.64±0.308) increased significantly. However, the mean micronucleus frequency in experimental group 2 (0.92 ± 0.129) and experimental group 3 (0.55 ± 0.046) compared with the control and sham exposed groups decreased significantly (p = 0.001).
Conclusion: Cell phones microwaves (940 MHz) induce chromosomal aberrations in bone marrow polychromatic erythrocytes of male Balb/C mice, but green tea has inhibitory effects and reduces chromosomal damage.
Hadi Ansari Hadipour, Ali Molaei Nezamabadi ,
Volume 17, Issue 3 (6-2014)
Abstract
Background: Our Previous studics hane been shown that iron ions produce oxygen free radical reaction products in the erythrocytes. The present study is evaluating the oxidative effects of aluminum in erythrocytes and plasma proteins and decline of antioxidant power of plasma in operating room personnel.
Materials and Methods: Blood obtained from operating room personnel. Erythrocytes and plasma were incubated aerobically in aluminum containing metal catalyzed oxidation (MCO) system. Total antioxidant capacity of plasma was estimated by FRAP assay (ferric reducing ability of plasma), plasma protein oxidation by carbonyl assay and structural changes in Hb were followed by spectrophotometric analysis and assessment of oxy-Hb, met-Hb and hemichrome.
Results: There was a significant relationship between optical density of Hb in 340 and 420 nm and between oxy-Hb and optical density of Hb in 420 nm(p<0.05) On the other hand, 68 to 56% decrease in total antioxidant power of plasma in control group and operating room personnel, results oxidation in plasma protein and carbonyl concentration was increased three fold.
Conclusion: These findings may be helpful in evaluating the antioxidant status and structural changes in Hb and plasma proteins in people who are exposed to aluminum especially operating room personnel.
Hamid Reza Dorostkar, Hadi Ansari Hadipour, Mohammad Taghi Goodarzi,
Volume 17, Issue 7 (10-2014)
Abstract
Background: There is a relationship between diabetes, dialysis and oxidative stress. The aim of this study was the comparison of structural changes in Hb, oxidative damages in plasma proteins, and antioxidant capacity in diabetic hemodialysis patients with those of control subjects.
Materials and Methods: In this experimental study, blood samples obtained from diabetic hemodialysis patients and control group. Oxidative damages in plasma proteins were determined by carbonyl assay and antioxidant power of plasma was performed by FRAP assay. Conformational changes in Hb were detected by spectrophotometric analysis. Blood glucose, urea, creatinine and uric acid in patients were determined usnig routine laboratory methods. Statistical analysis were performed by using regression analysis and t-test in SPSS20 software.
Results: This study showed a significant correlation between carbonyl content of plasma proteins and optical density of Hb at 630 and 275 nm which corresponds to structural changes in Hb. ferric reducing ability of plasma (FRAP), as an index of total antioxidant capacity of plasma was found to be enhanced significantly in diabetic patients receiving hemodialysis (from 1019.62±129 to 1354.54±129 molare, p<0.05).
Conclusion: The results obtained in the present study showed that inducible factors in diabetic hemodialysis patients contribute to plasma antioxidant activity and probably responsible for prevention of carbonyl formation and oxidative damages in hemoglobin.
