Showing 3 results for Egg
Mahzad Erami, Mahmood Saffari, Seyeid Ali Pourbakhsh, Seyeid Jamal Hashemi,
Volume 14, Issue 2 (5-2011)
Abstract
Background: Food contamination with fungi and the production of mycotoxins, such as aflatoxin, allow the toxins to enter human body. Continuous contamination with low doses of these agents can act as a major risk factor for hepatocellular carcinoma. Thus the present study was carried out to evaluate the detection of contamination in eggs with aflatoxin by PCR method.
Materials and Methods: In a cross-sectional study, a total of 144 suspicious and 211 intake eggs were collected and three samples of fungi including aspergillus niger, penicillium expansum, and fusarium verticillioides as negative controls and 14 samples of aspergillus flavus as positive controls were selected and examined using TLC and PCR. The results were analyzed through SPSS software.
Results: By PCR, neither aflR, omt-A, and ver-1, nor-1 was detected in intake eggs by PCR. Of the suspected eggs, four samples with nor-1, two samples with aflR, and two samples with omt-A could be detected. Three samples of the 14 strains of aspergillus flavus were shown to be positive through the use of TLC and the four primers. One strain of aspergillus flavus was positive with all of the four primers however, it was negative in TLC.
Conclusion: The findings of this study indicated that PCR is a sensitive, fast, and specialized technique, but it cannot detect the presence of the fungi before the appearance of colonization. Thus for indicating toxcification, other complementary tests are also required.
Sareh Mohamadi, Zahra Eslamirad, Reza Hajihossein, Mojtaba Didehdar,
Volume 18, Issue 12 (3-2016)
Abstract
Background: Toxocariasis is a parasitic disease caused by presence of Toxocara larva in human body. Soil as a reservoir maintained eggs of parasite and has important role in dissemination of disease because dogs and cats defecate eggs of parasite by feces. Despite there are large number of stray cats and dogs in urban areas such as Arak city, we need to collect information about soil pollution in public places. Therefore, the aim of this study was to evaluate soil contamination with Toxocara eggs in public parks in Arak city.
Materials and Methods: In this cross-sectional study, 60 soil samples were collected from 15 public parks in the Arak city during the summer 2015. The soil samples were provided by flotation method and examined by microscope.
Results: The results of this study showed that 4 (26.6%) of 15 parks were infected with Toxocara eggs. The highest rate of infection was observed in the walking and picnic site of parks. Also, in this study, two contaminated parks were located in the center and two parks were located in the southeastern of city.
Conclusion: Considering that soil contamination with Toxocara eggs was high in Arak city, it seems that the health authorities should design and implement programs in order to prevent human infection.
Mahdi Mosayebi, Reza Hajhossein, Mona Moghadasi,
Volume 26, Issue 6 (2-2024)
Abstract
Introduction: Fasciolosis is one of the most important parasitic disease common among both humans and livestock. That causes several health problems and economical losses in different parts of Iran. In order to control this disease, chemicals are used, which in some cases lead to resistance to the used substances and cause environmental problems. In this study, the effectiveness of the ethanolic extract of Lippia citriodora on the eggs of Fasciola hepatica is investigated.
Methods: Fasciola hepatica eggs were obtained from the liver and gall bladders of naturally infected sheep and kept at 4˚C until use. Ethanol extract of Lippia citriodora kunth was provided from dried leaves. The eggs were exposed to Different concentration of extract. Concentrations of Lippia citriodora extract (10, 20, 30, 40, 50, 75 and 100%). Control group eggs were placed in chlorine-free water. Egg and embryo development stages to complete and release miracidia were examined every day. Ethical considerations: This study was conducted after receiving the code of ethics (93-175-13) from the University Research Council.
Results: In concentration of 10 percent Lippia citriodora, miracidia was formed after 16 days in the egg. With increasing of concentration percentage of the extract, time of miracidia formation in eggs also rose. The time of miracidia formation between cases and control group is significantly different (P < 0.05).
Conclusions: Our study shows that extract of Lippia citriodora is an effective inhibiting factor against development of Fasciola eggs.
