Showing 8 results for Drug Resistance
Mohammad Amin Moosavi, Soroush Moasses Ghafary, Masood Asadi, Iraj Asvadi Kermani ,
Volume 14, Issue 4 (9-2011)
Abstract
Background: Leukemia is a malignant and progressive disease. Over-expression of inhibitors of apoptosis proteins (IAPs), such as survivin and its anti-apoptotic variants, including sur-ΔEx3, is the main cause of resistance to apoptotic effects of chemotherapy drugs. In the present study, the effects of CBX on apoptosis and expression level of survivin and sur-ΔEx3 and K562 cells (experimental model of chronic myeloid leukemia) were investigated.
Materials and Methods: In this experimental study, human K562 cells were cultured and exposed to CBX. Trypan blue exclusion test was used to evaluate growth inhibitory and viability effects of the drug. Fluorescent microscopy (acridine orange/ ethidium bromide double staining) and DNA electrophoresis were applied to the study of apoptosis. The expression level of survivin and sur-ΔEx3 was studied by semiquantative RT- PCR.
Results: The results showed that after the 48 h treatment of K562 cells with 150 µM CBX, significant growth inhibitory and apoptotic effects (up to 50%) were induced. In addition, after 2-4 h of treatment with CBX (150 µM), down-regulation of survivin and sur-∆Ex3 were observed. However, the expression level of survivin and sur-ΔEx3 increased to the level of control cells with longer treatment times (6-12 h).
Conclusion: Noticing the apoptotic and down-regulatory effects of CBX on survivin and sur-∆Ex3 expression, this drug can be used as a potential candidate for further studies on CML treatment, especially for inhibition of drug resistance in leukemia cells.
Neda Mokhberian, Forouzandeh Mahjoubi, Razieh Pour Ahmad, Mojtaba Alivandi,
Volume 16, Issue 10 (1-2014)
Abstract
Background: Multidrug resistance is the main reason for unsuccessful chemotherapy. The important reason of drug resistance is ATP dependent pumps shus as MDR1 that extrude drugs from the cell. MDR1 is high polymorphic. It seems that polymorphisms influent on gene expression and response to treatment. The aim of this study was investigation of C1236T polymorphism MDR1 gene and it’s association with response of treatment in childhood acute lymphoblastic leukemia.
Materials and Methods: In this descriptive study, C1236T polymorphism of MDR1 was investigated in 44 acute lymphoblastic leukemia childhood and 40 healthy individual by ARMS-PCR technique. Association of this polymorphism with response to treatment was also investigated. Data were analyzed using Chi-squre test and SPSS software. P values <0.05 were considered to be statistically significant.
Results: There was no significant difference in frequencies of C1236T polymorphism between patients and healthy group (p=0.876). Frequency of C1236T polymorphism of MDR1 between responder and non responder was not significant (p=0.304).
Conclusion: It seems that there is no correlation between C1236T polymorphism of MDR1 gene and response to treatment. So the role of C1236T polymorphism in gene expression MDR1 in childhood acute lymphoblastic leukemia and response to treatment is still controversial.
Farideh Tabatabaei Yazdi, Behrooz Alizade Behbahani, Maryam Heidari Sureshjani,
Volume 17, Issue 3 (6-2014)
Abstract
Background: The overuse of therapeutic antibiotics results in the drug resistance. The excessive use of antibiotics causes the mutations in the microorganisms and the emergence of new microorganisms which are resistant against the common antibiotics. With regard to limitations and known side effects of antibiotics, the exploring of antimicrobial compounds seems necessary. The purpose of this study is to evaluate the antimicrobial effects of aqueous and ethanolic extracts of Chevil against Staphylococcus epidermidis PTCC 1435, Yersinia enterocolitica PTCC 1221 and Enterobacter aeruginosa PTCC 1151 and to compare them with the common therapeutic antibiotics.
Materials and Methods: In this experimental study, after collecting plants from the highlands of Chaharmahal va Bakhtiari province, the extraction was carried out by the maceration method. To evaluate the antimicrobial activity, Disc diffusion test with Kirby-Bauer method was used. The Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentration (MBC) were determined by using the dilution method.
Results: The highest inhibition zone diameter in 40 mg/ml was related to Staphylococcus epidermidis and the minimum diameter in this concentration was related to Gram-negative bacteria, Enterobacter aeruginosa. MIC of aqueous and ethanolic extracts for Enterobacter aeruginosa 64 and 32 mg/ml and MBC of ethanolic and aqueous extracts of Enterobacter aeruginosa were 128 and 64 mg/ml respectively.
Conclusion: The ethanolic extract of the Chevil compared with the common therapeutic antibiotics had more inhibitory effect on studied bacteria. Furthermore, Chevil extracts showed greater inhibitory effect on Gram-positive bacteria in comparison with Gram-negative bacteria.
Faegheh Teymori, Nour Amirmozafari Sabet,
Volume 20, Issue 4 (7-2017)
Abstract
Abstract
Background: Acinetobacters are aerobic gram-negative bacteria which are distributed widespread in soil and water. The bacteria are isolated from cultured skin, mucous membranes, secretions and hospital environment. Acinetobacter baumannii, is a strain that more frequently isolated. Acinetobacter strains are often resistant against antimicrobial agents.
Materials and Methods: The method of this study was based on field, observation and test. On August and October 2015, samples were isolated from the soil and water of the Sadeghieh Square river in Tehran, respectively, and were transferred to the laboratory in the ice pack. 50 baumannii samples were isolated by biochemical methods (TSI, SIM, OF and gram test). November 1394, 100 clinical samples were isolated from Imam Khomeini hospital by biochemical method, and in the culture media Mueller Hinton agar plates were transferred to the laboratory. Antibiogram test for 150 baumannii samples was performed. Biofilms formation of Acinetobacter baumannii environmental and clinical samples was investigated by Congo red agar and culture plate methods.
Results: In all samples (clinical and soil), most of antibiotic resistance was 92% for imipenem and the resistance of water samples to imipenem was 99.9%. Biofilm formation by Congo red agar in water, soil, and clinical samles was resprctively 44%, 40% and 1%. All isolates were negative biofilm culture plate.
Conclusion: Considering Acinetobacter baumannii resistance to antibiotics and the lack of biofilm formation of in clinical and environmental isolates, it was concluded that there wasn’t any relationship between antibiotic resistance and biofilm formation.
Bita Kaviani, Hossein Sazegar, Noosha Zia-Jahromi, Farzane Mohamadi Farsani,
Volume 20, Issue 11 (2-2018)
Abstract
Abstract
Background: The aim of this study is to investigate the role of rs137852599 single-nucleotide polymorphism in the androgen receptor coding gene on drug resistance against treatment with Enzalutamide in individuals diagnosed with prostate cancer.
Materials and Methods: In this case-control study, the ARMS-PCR analysis was conducted on androgen receptor coding gene in 50 patients diagnosed with prostate cancer with drug resistance and on 50 patients diagnosed with prostate cancer without drug resistance. The statistical analyses were performed using the GeNePop server and then the results were investigated by the SISA server.
Results: The allele frequencies of A and C alleles in rs137852599 were 0.78 and 0.22 for drug resistant and 0.94 and 0.06 for non-drug resistance groups. The results indicated that there is a meaningful relationship between drug resistance and rs137852599 single-nucleotide polymorphism
(p = 0.020).
Conclusion: The existence of single-nucleotide polymorphisms may result in drug resistance in individuals diagnosed with prostate cancer. Therefore, investigation of the existence of such polymorphisms can be effective in prescription of suitable drugs for these patients.
Maryam Ghane, Fariba Adham,
Volume 22, Issue 6 (1-2020)
Abstract
Background and Aim: recent years, increase in extended-spectrum β-lactamases (ESBLs) producing Escherichia coli has led to limitations of treatment options. This study aimed to find the frequency of blaTEM and blaPER genes among ESBL producing urinary isolates of E. coli and detect their resistance pattern.
Methods & Materials: From January 2016 to February 2017, 972 urine samples from patients suspected of having urinary tract infections in three main hospitals and laboratories in Karaj were collected. Bacterial identification, antimicrobial susceptibility and ESBL production were performed according to the standard guidelines. Polymerase chain reaction was used for the detection of TEM and PER -lactamases.
Ethical Considerations: This study was approved by the Research Ethics Committee of Islamic Azad University of Tehran Medical Branch (Code: IR.IAU.TMU.REC.1396.274).
Results: Out of 972 samples, 500 cases were culture-positive for E. coli. Thirty-six percent (n =180) of the isolates were determined as ESBL-producer. Among ESBL positive isolates, the most susceptibility was observed in amikacin and imipenem (80 and 60% respectively). Resistance to trimethoprim/sulfamethoxazole,ciprofloxacin, tetracycline and gentamicin was 92.7%, 78.9%, 66.1% and 57.8%, respectively. All ESBL producers exhibited multidrug resistance. Among the ESBL-positive isolates, blaTEM gene was detected in 44.72% (n=85) of the isolates, but the blaPER gene was not found in any of the isolates.
Conclusion: The prevalence of multidrug resistant ESBLs producing uropatogenic E. coli is high. Continues monitoring of ESBL producers and their resistance patterns can help to reduce the spread of such resistant strains in the community.
Erfan Rezaei, Mojtaba Didehdar, Seyed Hamed Mirhoseini,
Volume 24, Issue 3 (8-2021)
Abstract
Background and Aim: Fungal infections are among the most critical and common issues for hospitalized patients, especially in intensive care units. This study aimed to determine the fungal contamination of indoor air and surfaces in sensitive wards of the Arak University of Medical Sciences educational hospitals and determine the drug susceptibility pattern of isolated species.
Methods & Materials: In this descriptive cross-sectional study, 63 air samples were taken from sensitive hospital wards using the one-stage Anderson method, and 63 surfaces samples were taken using wet cotton swabs and cultured in saprodextrose agar medium containing chloramphenicol. Identification of the genus and, as far as possible, the species of fungi was performed using the culture method on the slide. Drug susceptibility testing was performed on isolated species by broth microdilution method (CLSI-M38A2 standard).
Ethical Considerations: This study was approved by the Research Ethics Committee at Arak University of Medical Sciences (Code: IR.ARAKMU.REC.1395.315).
Results: From the total samples, 18 species of fungi were isolated. These included: Aspergillus niger (8), Aspergillus flavus (4), Aspergillus fumigatus (2), Rhizopus spp. (2), Mucor spp. (1) and Fusarium spp. (1). In the drug sensitivity assay, instances of resistance included: Partial sensitivity of Aspergillus fumigatus to Itraconazole (1), Partial sensitivity of Aspergillus niger to Ketoconazole (1), and Resistance of Aspergillus niger to Itraconazole (1).
Conclusion: The pattern of nosocomial fungal infection with pathogenic fungi and the drug susceptibility pattern of these organisms in other regions of Iran and the world is relatively consistent with the present study results. And drugs listed in global guidelines for treating these infections, such as voriconazole and caspofungin in the treatment of invasive aspergillosis and amphotericin B in the treatment of invasive mucormycosis and Fusarium wilt, are now effective drugs.
Ali Moradpoor Shamami, Dr Masumeh Anvari, Seyedeh Tooba Shafighi, Hadi Sedigh Ebrahim-Saraie,
Volume 25, Issue 5 (12-2022)
Abstract
Introduction: Considering the importance of urinary tract infections caused by uropathogenic Escherichia coli (UPEC) in the medical field, this study aimed to investigate serogroups O25 and O16 and the pattern of antibiotic resistance among UPEC isolates obtained from hospitalized patients with urinary tract infections (UTIs) in Rasht hospitals.
Methods: A total of 110 urine samples were collected from patients with UTIs referred to selected hospitals in Rasht. The disk diffusion method, as recommended by the CLSI, was used to determine the pattern of antibiotic susceptibility. Serogroups O25 and O16 were detected using specific primers.
Results: Among the studied samples, 36.4% (40/110) were men and 63.6% (70/110) were women. Based on the antibiotic susceptibility pattern, a high level of antibiotic resistance was observed against nalidixic acid (81.8%) and co-trimoxazole (78.2%), while the most effective antibiotics were amikacin (85.5%) and nitrofurantoin (83.6%). In addition, multi-drug resistant phenotype was found in 72.7% (110/80) of UPEC isolates. According to PCR results, the frequency of serogroups O25 and O16 was 36.4% and 17.3%, respectively. Both serogroups had the highest resistance to nalidixic acid and co-trimoxazole, while the lowest resistance in serogroup O25 to nitrofurantoin (20%) and amikacin (14.3%) and in serogroup O16 to imipenem (5.3%) and nitrofurantoin (10.5%).
Conclusions: This study showed that the high prevalence of MDR strains among UPEC strains is very worrying and professionals should be very careful in prescribing antibiotics for patients. Like most studies, the frequency of serogroup O25 was high, and probably, this serogroup can play a role in causing urinary tract infections and antibiotic resistance of UPEC strains.
