Esmaiel Saberfar, Zahra Goodarzi, Ali Najafi,
Volume 15, Issue 8 (1-2013)
Abstract
Background: Influenza type A virus is one of the most important viral agents in human respiratory diseases. The genetic variability of the influenza viruses leads to the incidence of new epidemics worldwide. Hence, there is a growing need for rapid and effective new methods capable of detection and differentiation of influenza virus circulating strains. This study was done to develop a method for rapid differentiation of the subtypes of influenza type A virus. Materials and Methods: In this experimental study, reverse-transcription and polymerase chain reaction (RT-PCR) were performed using a primer set based on M gene of H1N1, H3N2, H5N1, and H9N2 influenza subtypes. Then the amplified fragments were subjected to digestion using subtype specific restriction endonuclease enzymes. Results: The results of PCR reaction showed that the primer pair of the M gene was specific and capable of amplifying all influenza subtypes understudy. Also, different restriction fragment length polymorphism patterns (RFLP) were generated using enzyme digestion reaction on the amplified segment of M gene. Conclusion: RT-PCR and RFLP analysis of the M gene can be employed as a useful method for differentiating influenza virus subtypes
Malahat Amani, Hossein Alizade, Esmaeil Shiri,
Volume 20, Issue 10 (1-2018)
Abstract
Abstract
Background: The exact diagnosis of schizophrenic disorder from schizoaffective disorder has always been a challenge for therapists due to the comorbidity of many signs and symptoms. The purpose of this study is to investigate the differential diagnosis of schizophrenia disorder from schizoaffective disorder, focusing on the emotional content of their memories.
Materials and Methods: The present study is a causal-comparative study on 23 male and female patients with schizophrenia and schizoaffective disorder (without any comorbid diagnosis). Using a clinical interview, the emotional events and memories of their lives, as well as the amount of affective and emotional words used by these patients in expressing their memories, have been assessed. To determine the differences between the two groups, the data were analyzed by Chi-square test.
Results: Results showed that men with schizophrenia had a significant difference in expressing the amount of emotional events in their lives, as well as in expressing affective and emotional words compared to schizoaffective men. But, this difference was not observed in schizophrenic and schizoaffective women.
Conclusion: The findings of this study suggest that differential diagnosis of schizophrenia and schizoaffective disorder can be made with emphasis on the emotional content of memories, especially in men.
