Background: Male fertility depends on the proper function of a complex system of organs which plays an important role in spermatogenesis. In this study the effects of sulpiride-administration were assessed by means of sperm parameters and in vitro fertilization potential.

Materials and Methods: In this experimental study thirty adult male mice were divided into 3 groups as test, control-sham and control. The test group were injected with 40 mg/kg sulpiride solution daily for 45 days IP. Sham mice were injected by solvent only. After 45 days, all mice were dispatched by cervical dislocation consequence of unconsciousness. Cauda epididymis were used to collect sperm cells and assess their motility, viability and DNA integrity. The rate of in vitro fertilization and embryonic development were also examined.

Results: In comparison with sham and control groups, sperm motility and viability rate showed a significant reduction in the sulpiride-administered animals. Rate of DNA damage increased which gives rise to a remarkable reduction of fertilization rate, zygote division, blastocysts number, and significant increase of arrested embryos in sulpiride treated mice (p<0.05).

Conclusion: Data suggest that following sulpiride-induced hyperprolactinemia, induction of spermatogenesis dysfunction, causes low sperm quality that accompanies a significant lower fertility potential and embryonic development in comparison with the sham and control groups.

Abstract

Background: Operating room personnel are subject to occupational hazards which could lead to an increase in free radicals and develop various diseases. The aim of the present study was to determine the effect of consuming green tea on the improvement of the blood oxidative biomarkers in operating room personnel who are exposed to anesthetic gases.

Materials and Methods: This study was a before-after clinical trial which was conducted on 24 operating room personnel. They were invited to consume 4 cups of a green tea beverage, prepared from 3 g of green tea leaves in 300 mL of boiled water (at 80˚ C), daily for 8 weeks. Then, Myeloperoxidase (MPO), DNA damage, Glutathione Peroxidase (GPx), and Superoxide Dismutase (SOD) in the plasma were measured in order to evaluate the level of oxidative stress biomarkers before and after consuming green tea.

Results: Green tea consumption by operating room personnel brought about a significant increase in glutathione peroxidase and superoxide dismutase and a considerable decrease in myeloperoxidase and DNA damage.

Conclusion: According to the results of this study, green tea consumption as an antioxidant supplement by operating room personnel, who are regularly exposed to anesthetic gases, can minimize oxidative stress and DNA damage considerably. Thus, it is advisable for operating room personnel to consume green tea as a natural antioxidant supplement.