Showing 7 results for Cytotoxic
Mehdi Mosayebi, Ehsan Ghaznavi, Abdolhossein Dalimi, Mohamad Moazeni, Ghasem Mosayebi, Mahmoudreza Khazaii,
Volume 9, Issue 3 (9-2006)
Abstract
Introduction: There is difference between susceptibility or resistance to infectious diseases such as cystic and alveolar Eclinococcosis in human and animals, that is due to the difference between individual host factors and immunologic responses. This study is done to investigate the resistance and susceptibility markers (HLA) in Hydatid patients and healthy persons. Materials and Methods: This analythical (case-control) study is carried out on 60 patients with confirmed cystic echinococcosis and 30 healthy individuals living in Arak. Blood samples were gathered and tested by microlymphocytotoxicity method. At first diagnostic kits with specific antiserusms for each antigen (28 antigens) were provided and then lymphocytes were separated. After dye and stabling with formalin and based on cells morphology, results were seen by invert microscope. Data was analyzed using Odds Ratio, Relative Risk, Preventive Fraction, Aetiologic Fraction and Chi square test. Results: Results showed that HLA-A1 was significantly higher in patients (p<0.05), and people having this antigen are more susceptible for the infection. In spite, HLA-A10 was higher is healthy individuals (p<0.05) and have a preventive effect in disease involvement. Other investigated antigens had no signigicant difference in the two groups. For more accurate results molecular investigation is needed. Conclusion: In individuals having HLA-A1 there is more chance for cyst growth in confronting hydatosis and this individuals are more susceptible to the disease. But in individuals having HLA- A10 there is less chance for cyst growth in confronting hydatosis and this antigen have a preventive effect against hydatid cyst.
Elahe Aslani, Nooshin Naghsh, Monire Ranjbar,
Volume 16, Issue 10 (1-2014)
Abstract
Background: Chronic myeloid leukemia (CML) is a malignant clonal disorder of hematopoietic stem cells which results in increase of myeloid cells, erythroid cells and platelets in the peripheral blood and hyperplasia in bone marrow. The research evaluates the cytotoxic effect of hydro-alcoholic extract of M.polegium before flowering aerial organs on K562 cell line as a model of chronic myeloid leukemia.
Materials and Methods: In this experimental trial, Leaves and stems of M. pulegium before flowering collected from Afoos city and extraction using maceration method. K562 cells were cultured and treated with concentrations of extract (12.5-100 &mug/ml) and different times (24,48,72 hour). Cytotoxicity of M. pulegium before flowering extract against K562 leukemia cells was estimated by the MTT test method. The absorbance was measured using an ELISA plate reader at 540 nm. Survey on data accomplished with the use of SPSS15 software and one-way ANOVA test analysis and Tukey test and p<0.001 was considered significant.
Results: Hydro-alcoholic extract of Mentha pulegium before flowering showed the highest cytotoxic effect (IC50=50 &mug/ml) and 72 hour after treatment on K562 cell line .in other words, hydro-alcoholic extract of Mentha pulegium before flowering extracts a dose and time dependent cytotoxic effect on K562 cell line.
Conclusion: Considering the cytotoxic effect of hydro-alcoholic extract of M.polegium before flowering aerial organs on K562 cells, the plant can be considered as a potential candidate for further studies on CML treatment.
Fatemeh Keikhaei, Nooshin Naghsh, Mehrdad Modaresi,
Volume 17, Issue 6 (9-2014)
Abstract
Background: Leukemiais a malignant and progressive disease of the Hematopoietic tissues of the body. The pistacia atlantica tree base, the geographic in large areas of the Mediterranean and the Middle East is growing. K562Cell class is considered as laboratory model of chronic phase of human CML. We compared the growth inhibitory effects SUZIN as a chemical compared with pistacia atlantica as a combined Zn plant antioxidant capacity in reducing cancer has been studied.
Materials and Methods: Pistachio nut was collected from around Kerman, then they were dried in room temperature and extraction was performed for 48 hours by maceration method. K562 cell class was incubated in medium RPMI-1640 fortified with 10%(v/v) FBS and 50% Streptomycin-Penicillin. Cytotoxic effect of hydro-ethanolic extract of pistacia atlantica against cancer cells K562 was evaluated in three interval by MTT method. Light absorbance by Eliza device was measured in wave length 540 nm. Statistical analysis was performed using SPSS15 software and ANOVA test.
Results: Pistacia atlantica in 24h and in concentration 100&mug/ml andSuzin in48h and in 12.5 &mug/ml,72 h and in 50 &mug/ml induced growth inhibition half of the cells were K562. Results obtained from changes in cell morphology influenced by hydro-ethanolic extract of pistacia atlantica and SUZIN suggest abnormal transformation of cells that probably represents apoptosis and necrosis.
Conclusion: Time and concentration against cytotoxic effect of Pistacia atlantica have the combined effect. Whileiron supplementation, alone time is due. Special concentration of pistacia atlantica having high antioxidant capacity with the Suzincan be considered as a potential target for inhibiting K562 cells in treatment of blood cancer.
Bagher Seyed Alipour, Najmeh Barimani, Abbasali Dehpour Jooybari, Seyed Mohammad Hoseini,
Volume 17, Issue 11 (2-2015)
Abstract
Background: Nanomaterials have gained increasing attention because of their novel properties, including a large specific surface area and high reaction activity. This study was designed to investigate the cytotoxic effects of CuO nanopaticles on brain, spleen, and embryo NMRI pregnant mice.
Materials and Methods: In this experimental study, forty two female NMRI mice of (weighting 30±3.0 g) were randomly divided into six groups (four experimental groups, one sham group and one control group).The experimental mice on days 3 and 12 of pregnancy received CuO nanoparticle with concentrations 50, 100, 150, 200 mg/kg intraperitoneal injection. On day 17 pregnancy, brain, spleen and fetus weights were measured.Tissues for histopathological evaluation were stained with hematoxylin and eosin.
Results: Based on the macroscopic observations of embryos weight with increasing concentration of nanoparticle compared to control reduces its toxicity increased (p&le0.05). Spleen only at concentration of 600 mg/kg showed significant changes compared to control (p&le0.05). Histopathologic examination on brain and spleen following IP administration of CuO nanoparticle showed signs of cytotoxicity (congestion, necrosis, inflammatory cell infiltration, vacuolar degeneration) and (congestion, necrosis, increased hemosiderin) compared to control group, respectively.
Conclusion: The present study clearly showed that CuO-NPs can produce the histopathological abnormalities on brain and spleen tissues of NMRI mice in a dose-dependent manner.
Sanaz Alizadeh, Naser Aghdami, Bagher Seyed Alipour,
Volume 20, Issue 1 (4-2017)
Abstract
Abstract
Background: Copper nanoparticles (Cu NPs) induced angiogenesis, has been adapted to respond the most important challenging in wound healing. But due to the toxicity of nanoparticles, the nontoxic concentrations is important. The aim of this study was to determine the concentration and size of copper nanoparticles for investigating the effect of its cytotoxicity on the endothelial cell.
Materials and Methods: In this study, we exposed Cu NPs (40nm) with concentrations of 1, 10, 100 μM and 1 ,10 mM to endothelial cells and evaluate its viability effect after 24, 48 and 72 hours, according to the MTS) Methy Thiazol Tetrazolium (assay. Its optical density was determined using an ELISA reader and then was recorded.
Results: The findings demonstrated that Cu NPs was significantly (p<0.05) cytotoxic in concentration higher than 100 μM and cell viability was significantly increased following 48 and 72 hours in all concentrations, so that, the most difference was seen in 100 µM concentration. The IC50 values of Cu NPs at incubation time 24, 48 and 72 hours were 31.44, 36.67 and 29.38 μM.
Conclusion: The results showed that different concentration of Cu NPs in the 48 and 72 hours didn’t cause any cytotoxicity effect, but it stimulated endothelial cell proliferation. Therefore, Cu NPs with dose and time dependent effect has been increased endothelial cell proliferation.
Mahmoud Bahreloloum Tabatabai, Mohammad Mirjalili, Fatemeh Yazdiyan, Seyedhossein Hekmatimoghaddam,
Volume 22, Issue 1 (4-2019)
Abstract
Background and Aim: The aim of this study was to assess the applied characteristics of wound covers containing nanoliposomic essential oil of ajwain, with suitable antimicrobial properties and lack of cytotoxicity.
Materials and Methods: Liposomal formulations of the ajwain essential oil containing DSPE-PEG, cholesterol, span60 and SPC80 were prepared using a thin layer method. The rooting and spray methods on a cellulose fabric were used to produce skin wound cover. In addition to in vitro intracellular penetration and measurement of minimum inhibitory concentration of the product, textile characteristics, antimicrobial activity and 96 hours release of the essence in the wound cover were studied.
Ethical Considerations: In this study, all principles of research ethics were considered.
Findings: The loading efficiency of the liposomal formulation was more than 85%. The small particle dispersion index (PDI = 0.02) in the form of the PEGylated formulation indicates optimal dispersion of the particles which reduces the buildup of the drug in the cutaneous application. The standard AATCC microbial test showed inhibitory effect of the wound cover on bacteria, especially E. coli. Textile tests indicated acceptable properties of the produced wound cover, too.
Conclusion: Altogether, this wound cover showed acceptable features in combating the two selected bacteria responsible for infectious skin ulcers.
Sahar Dehghani, Leila Rouhi, Noosha Ziya Jahromi, Reza Dehghani, Khalil Khashei Varnamkhasti,
Volume 24, Issue 2 (5-2021)
Abstract
Background and Aim: Proliferate potential differentiate into different cell lineages and high self-renewal of Mesenchymal Stem Cells (MSCs); thus, they are ideal tools for regenerative medicine. However, a leading problem is an oxidative stress in the target tissue and the apoptosis of transplanted stem cells before tissue repair. The pretreatment of stem cells with antioxidants may make them resistant to oxidative stress. Ginger is the main medicinal plant with antioxidant properties. This study explored the antioxidant effects of ginger extract on bioavailability and oxidative stress-induced apoptosis in human adipose tissue-derived mesenchymal stem cells and rat bone marrow examined.
Methods & Materials: In this study, human adipose tissue-derived mesenchymal stem cells and rat bone marrow were cultured in a DMEM medium with 20% FBS. The explored cells were incubated for 4 and 6 hours for pretreatment with different concentrations of ginger extract (50, 100, 200, & 400 mg/mL); then, they were treated with 200 μM H2O2 for 2 hours. Bioavailability was analyzed by ELISA reader using an MTS kit and apoptosis was analyzed by flow cytometry using an Annexin V-FITC/PI kit into the manufacturer’s protocol at both times. The obtained data were analyzed by Analysis of Variance (ANOVA) using SPSS.
Ethical Considerations: This study was approved by the Ethics Research Committee of Shahrekord Branch, Islamic Azad University (Code: IR.IAU.SHK.REC.1397.028).
Results: The MTS results indicated a dose- and time-dependent manner increase in the bioavailability of human adipose tissue-derived mesenchymal treated stem cells. Ginger extract treatment also dose- and time-dependently decreased the rate of apoptosis in rat bone marrow mesenchymal stem cells.
Conclusion: Ginger extract, by reducing the oxidative stress in mesenchymal stem cells, elevates their lifespan in the target tissue, and increases the efficiency of these cells in tissue regeneration.
