Showing 10 results for Culture
Amir Ghaemi, Mohammad Fashbaf, Hoorie Soleimanjahi, Reza Omidbeigi, Taravat Bamdad, Davoud Hekmatpour, Kasra Hamdi,
Volume 10, Issue 2 (6-2007)
Abstract
Introduction: Nowadays, the attention of researchers has been focused on natural medicine in order to avoid the detrimental side effects of chemical drugs. In this study we assessed the effect of root extract of Tagetes minuta against HSV-1 and HSV-2. Materials and Methods: This research is an experimental study. Root extract of Tagetes minuta was obtained with 70% ethanol by maceration. Vero cells were grown in DMEM containing 5% fetal bovine serum. Serial dilutions of extracted suspension (1/10, 1/20, 1/40, 1/80, 1/160) were incubated by the exact titer of viruses and monitored for antiviral activity of extract. Data was analyzed using Doncan test. Results: Root extract obtained from Tagetes minuta significantly has antiviral activity against HSV-1 and HSV-2. This extract has more effect on HSV-2 than HSV-1. This study indicates that antiviral activity of the extract varies between different concentrations and the optimum antiviral activity on both viruses was obtained using 1/10 concentration. Conclusion:The results of this investigation showed that root extract of Tagetes minuta have good antiviral potenoial against HSV-1 and HSV-2, a good source of drug for treatment of diseases due to HSV-1 and HSV-2.
Parsa Yousefi Chaijan, Fatemeh Dorre, Zahra Moghaddasi, Maryam Mashayekhi,
Volume 13, Issue 3 (9-2010)
Abstract
Background : Urine tract infection(UTI) among children is the most prevalent bacterial infection and the second prevalent infection after Viral Flu.Regarding this cfact that urine culturing has very important role in diagnisis and perseverance of this disease and sampling method has significant effect on this disease remedy.
In this study the effect of genital area ablution on decreasing the contamination of urine culture is examined.
Methods and Materials: This study is in the form of case_controlled.In this study 620 little girls (3-12 years –old) referring to Amir Kabir children special clinic are examined.Population under investigation was cpatients who had not history of taking antibiotic during last seven days, sondage and genital anomaly and inflammation and genital discharge.
Patients were randomly entered in two different groups one with ablution and the other without ablution.After data collection, available inputs were statistically analyzed with SPSS and the effect of genital area ablution was determined on decreasing the contamination of urine culture.
Results: Among 310 patients in with_ablution group 11 patients were reported UTI(3/7%).3 patients were contaminated (1%),296 patients were normal (95/3%).Among 310 patients in the second group,14 patients were reported UTI(4/7%),6 patients were contaminated(2%),290 patients were normal(93/3%).Contamination rate in with_ablution group were 1% and in the other group was 2%Based on Pvalue :0/491 in both groups no significant statistically difference were found between these two groups and the analysis result distribution in both groups in a=0/05 level were statistically the same.
Conclusion: In both group no significant statistically difference were found between these two groups ,so we concluded that the genital area ablution doesn’t have such an effect on decreasing the contamination of urine culture and we don’t suggest ablution of genital area in children before providing urine sampling.
Fatemeh Piryaei, Mina Ramezani, Fahimeh Piryaei,
Volume 14, Issue 2 (5-2011)
Abstract
Background: Noticing the practical significance of stem cells, this study was conducted to culture and screen bone marrow mesenchymal stem cells derived from Raf and Hilline chicken strains and investigate the effect of age and race on the morphology and differentiation of the generated cells.
Materials and Methods: In this fundamental study, bone marrow cells from 3 to 25 day-old Raf and Hiline chicken strains were cultured in low glucose DMEM, 10% BFS. Then third passage bone marrow cells of the two strains were compared in terms of morphology, differentiation to bone, cartilage, and adiposity. Data were analyzed through SPSS software.
Results: In culturing Raf chicken derived bone marrow cells, in contrast to Hiline chicken strain, colonization took place and they almost had a better fibroblastic morphology. The results indicated higher yields of differentiation to bone, cartilage, and adipose tissues in Raf chicken derived bone marrow cells than Hiline chicken. These differences were statistically significant. Also, 15 days was the most suitable age for screening the mesenchymal stem cells of chicken.
Conclusion: Screening and proliferation of mesenchymal stem cells from 15-day old Raf chicken bone marrow cells are good resources for differentiation and purification of chicken bone marrow mesenchymal stem cells.
Peyman Abdollahzadeh, Reza Shapouri, Shahrzad Nasiri Semnani, Hamed Alizadeh,
Volume 14, Issue 7 (2-2012)
Abstract
Background: Brucellosis is caused by brucella which is a facultative intracellular pathogen invading both professional and nonprofessional phagosytic cells. Eucalyptus globulus is one of the most widely used medicinal plants in folk medicine throughout the world. The aim of this study was to evaluate the antibacterial effects of Eucalyptus globulus extracts on intramacrophage Brucella melitensis 16M.
Materials and Methods: In this experimental study, after preparing aquatic, ethanolic, and acetonic extracts of Eucalyptus globules, the effect of the extracts on intramacrophge survival of B. melitensis 16M obtained from cell culture of Balb/c mice peritoneal macrophages was studied. In order to do this, after lysis of macrophages, through preparation of serial dilutions and culture on Mueller Hinton agar medium, the number of colonies grown was counted.
Results: The maximum antimicrobial activity of Eucalyptus globulus extracts on intramacrophage B. melitensis 16 M were in 1:40 dilution (21.62 mg/ml) of the aquatic extract, 1: 640 dilution (1.26 mg/ml) of the ethanolic extract, and 1:320 dilution (2.59 mg/ml) of the acetonic extract after 24h.
Conclusion: Aquatic, acetonic, and ethanolic extracts of Eucalyptus globulus possess antimicrobial properties against intramacrophage B. melitensis 16M and ethanolic extract has the most effective antimicrobial activity on intramacrophage Brucella melitensis therefore, these extracts can be useful in treatment of brucellosis.
Hosein Sohrabi, Mohammad Reza Sarookhani, Akram Ezani,
Volume 16, Issue 8 (11-2013)
Abstract
Background: Vulvovaginal candidiasis is a common problem in women. The purpose of this study was to compare two identification methods new PCR analysis (with DNA extracted from samples) and conventional culture technique in detection of Candida species in vulvovaginal samples.
Materials and Methods: In this experimental-analytical study, 150 women of fertility ages participated and two vaginal discharge samples were collected by speculum. One sample used for direct DNA extraction as well as PCR and the other was used for culture and phenotypic evaluations. Phenotypic evaluations waere performed by germ tube and chlamydospore formation and specially culture in chrome agar medium to detect color of the colonies. PCR was performed by DNA extracted from samples as templates and finally size of Candida specific amplicons was determined.
Results: From 150 samples, 87 in culture and 127 in new PCR technique were positive. In culture method, from total 87 Candida species , 73 C. albicans, 12 C. glabrata and 2 C. tropicalis were isolated whereas in new PCR technique, from total 127 candida species 107 C. albicans, 18 C. glabrata and 2 C. tropicalis were identified. Concordance of the two methods were calculated as 68 percent.
Conclusion: The new molecular method (innovative PCR) has the potential to rapidly and accurately diagnose Candida vulvovaginitis in patients and can be used for diagnosis of Candida species in clinical specimens.
Zahra Naseri, Mohammad Yousef Alikhani, Seyyed Hamid Hashemi, Mohammad Reza Arabestani,
Volume 17, Issue 5 (8-2014)
Abstract
Background: Brucellosis is a major cause of zoonosis disease and is endemic in hamadan Province in Iran. The purpose of this study was to isolate Brucella species from brucellosis patients and identify different species of this bacterium in order to determine the prevalence of the species.
Materials and Methods: This study was descriptive- cross sectional and fifty blood samples were obtained from brucellosis patients with clinical symptoms. The samples were cultured in BACTEC system and incubated for 14 days. Then, the samples were cultured on Brucella agar and biochemical tests were done for identification of bacteria. Finally, Polymerase Chain Reaction (PCR) applied for confirmation and isolated identification with specific primers.
Results: Seven Brucella strains were isolated from 50 blood samples of the patients with brucellosis by blood culture and PCR. The PCR results on blood specimens showed 4 positive in spite of the negative results of blood culture. PCR and biochemical methods revealed that all the 11 isolated bacteria were Brucella Melitensis.
Conclusion: This study was designed to evaluate PCR technique as a diagnostic tool for brucella spp in comparison to conventional techniques. This study showed a high prevalence of brucellosis due to Brucella Melitensis in Hamadan Province and efforts in this region should be aimed at the eradication of this bacterium.
Ali Reza Morad Abadi, Mohammad Arjomandzadegan, Navid Emami, Manijeh Kahbazi, Azam Ahmadi, Saeed Falahat, Seyyed Hossein Hosseini, Mehdi Kargaran, Parisa Khosravi,
Volume 21, Issue 4 (8-2018)
Abstract
Background and Aim: Ziehl Nelson staining, fluorescent and also culture are the standard methods for the diagnosis of tuberculosis. In this study, the performance of conventional cultivation methods was compared with Flash PCR.
Materials and Methods: A total of 56 sputum samples from patients with suspected tuberculosis in Tuberculosis Center of Arak city were collected and Ziehl–Neelsen and culture in Löwenstein–Jensen medium were accomplished. Moreover, DNA from all of the 56 sputum samples was extracted by Chelex100 method. Molecular evaluation was accomplished by Flash PCR kit containing probes and primers for gene amplification IS6110. Positive and negative controls together with samples were used in a MTC410 apparatus for amplification. FD-12 apparatus was used to evaluate the results. In addition, electrophoresis on agarose was used for confirmation of the results.
Findings: From 56 sputum samples of suspected TB patients, 20 samples were positive and 36 samples were negative on microscopic evaluation and culture methods. FLASH-PCR molecular analysis showed that all of 20 positive samples were positive in molecular methods, too. On the other hand, three of sputum samples that were negative by culture and staining were positive in FLASH-PCR method. One of these 3 patients, received Isoniazid, pyrazinamide and ethambutol antibiotic by responsible medicine. All results were confirmed using conventional electrophoresis.
Conclusion: In some negative samples, possibly because of the small number of bacteria in sample or a defect in the sampling, the Flash PCR may due good advantages. Therefore, due to the low cost, this method is recommended for routine use.
Nahal Moharekpour, Vahab Habibipour, Davood Shamci,
Volume 25, Issue 2 (5-2022)
Abstract
Background and Aim Crisis management is an important field of management, especially in the field of health. This study aims to investigate the factors affecting the development of disaster management mechanisms in hospitals of Arak, Iran.
Methods & Materials In this regard, 240 questionnaires were distributed among hospital and nursing managers in Arak. The questionnaire had acceptable reliability with Cronbach’ alpha value of 0.897.
Ethical Considerations This study was approved by the ethics committee of Arak University of Medical Sciences (code: 1274.1401.REC.ARAKMU.IR).
Results Then, by using structural equation modeling in LISREL software, the designed model was analyzed. Finally, by using statistical tests, the research hypotheses were examined.
Conclusion The results showed the significant effect of three factors of “lack of stability in business environment”, “risk taking”, and “organizational culture” on the development of the mechanism of crisis management.
Mahmood Haghiri, Vahab Habibpour, Nahal Moharekpour, Morteza Ghasemi,
Volume 25, Issue 2 (5-2022)
Abstract
Background and Aim This study aims to investigate the effect of nurses’ autonomy on the quality of patient care based on Kramer and Schmalenberg’s model
Methods & Materials This is a causal-comparative study. Participants were 101 nurses of Imam Khomeini Hospital in Arak, Iran from the intensive care unit and internal medicine, surgery, pediatrics, gynecology, orthopedics, and emergency departments. The data collection tools were Moghimi and Ramazan’s patent care quality questionnaire and an autonomy assessment questionnaire based on Kramer and Schmalenberg’s autonomy model. The data were analyzed in SPSS software.
Ethical Considerations This study was approved by the ethics committee of Islamic Azad University of Arak Branch (Code: IR.IAU.ARAK.REC.1397.66).
Results The mean autonomy score of nurses was 3.16 ±0.35, indicating a desirable level. Nurses of all departments had limited autonomy. In the internal department, nurses had unsanctioned autonomy, while in the surgical department, they had autonomous nursing care action. The regression models were significant for all departments except for the internal department. With an increase of one unit in limited autonomy in the intensive care unit, autonomous nursing care in the surgical department, and limited autonomy in pediatrics, gynecology, orthopedics, and emergency departments among the nurses, the quality of care can be increased by 0.253, 0.187, 0.522, 0.205, 0.466 and 0.129, respectively.
Conclusion Nurses’ autonomy can increase the quality of patient care. The nursing managers need to empower nurses and provide an environment for them to apply their knowledge in practicing autonomy.
Mohammad Reza Heidari, Mehrdad Goudarzvand Chegini, Hamid Reza Rezaei Kelidbari, Morad Rezaei Dizgah,
Volume 25, Issue 2 (5-2022)
Abstract
Background and Aim The internationalization of higher education is an effective strategy to meet the diverse needs of a society. One of the factors determining the failure or success of internationalization is the organizational culture of universities. The present study aims to investigate the role of organizational culture-related factors in the internationalization of Iranian universities.
Methods & Materials This is a quantitative-quantitative study. Participants were 124 Iranian experts in higher education and those involved in internationalization of universities. A researcher-made questionnaire was used as a self-report tool with close-ended questions. In the quantitative stage, structural equation modeling and first- and second-order factor analysis were performed in SPSS and AMOS software.
Ethical Considerations This study was approved by the ethics committee of Islamic Azad University, Rasht Branch (Code: IR.IAU.RASHT.REC.1400.035)
Results The ranking of factors affecting the internationalization of Iranian universities showed that the factor with the highest factor loading was “communication & interaction” (1.03) and the factor with the lowest factor loading was “human/physical/financial resources” (0.87).
Conclusion Communication/interaction of universities with other education centers, as an organizational culture-related factor, plays an important role in the internationalization of Iranian universities.
