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Background: Brucellosis is caused by brucella which is a facultative intracellular pathogen invading both professional and nonprofessional phagosytic cells. Eucalyptus globulus is one of the most widely used medicinal plants in folk medicine throughout the world. The aim of this study was to evaluate the antibacterial effects of Eucalyptus globulus extracts on intramacrophage Brucella melitensis 16M. Materials and Methods: In this experimental study, after preparing aquatic, ethanolic, and acetonic extracts of Eucalyptus globules, the effect of the extracts on intramacrophge survival of B. melitensis 16M obtained from cell culture of Balb/c mice peritoneal macrophages was studied. In order to do this, after lysis of macrophages, through preparation of serial dilutions and culture on Mueller Hinton agar medium, the number of colonies grown was counted. Results: The maximum antimicrobial activity of Eucalyptus globulus extracts on intramacrophage B. melitensis 16 M were in 1:40 dilution (21.62 mg/ml) of the aquatic extract, 1: 640 dilution (1.26 mg/ml) of the ethanolic extract, and 1:320 dilution (2.59 mg/ml) of the acetonic extract after 24h. Conclusion: Aquatic, acetonic, and ethanolic extracts of Eucalyptus globulus possess antimicrobial properties against intramacrophage B. melitensis 16M and ethanolic extract has the most effective antimicrobial activity on intramacrophage Brucella melitensis therefore, these extracts can be useful in treatment of brucellosis.

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Background: Brucellosis is a debilitative disease that imposes heavy costs on the economy and society. Therefore, using the most accurate and efficient method to diagnose this disease is essential. In Iran, Brucella melitensis is the common causative agent for brucellosis and BP26 protein of this bacterium has a good level of antigenicity. Thus, the aim of this study is to produce Brucella melitensis recombinant BP26 protein with a PET28a expression vector.

Materials and Methods: In this applied-fundamental study, genomic DNA was isolated from bacterial culture through proteinase K (pK) and phenol/chlorophorm protocol. Then, two pairs of primers were designed based on the known sequence in the gene bank for amplification of Brucella melitensis bp26 gene and PCR reaction was set up and optimized. The PCR product was cloned first into PTZ57R/T vector and accessed on the PET28a vector and sequenced. The recombinant vector was transformed and expressed into E. coli BL21 (DE3). Then, the recombinant protein was purified with Ni-NTA column of chromatography against His tag.

Results: The size of PCR product was in accordance with the part of bp26 gene size in the gene bank. The bp26 gene without adding IPTG had little expression and 3 hours after adding IPTG with a 1 Mm concentration to culture media, extreme expression was observed.

Conclusion: The production of recombinant BP26 protein from isolated Brucella melitensis native to Markazi province was done. Noticing the importance of BP26 protein and its significance for future studies on providing brucellosis diagnosis kits, its production was made possible.

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Background: Brucellosis is one of the most important diseases among humans and animals. Clinical management of brucellosis due to an increased rate of treatment failure and recurrence is extremely worrying. The aim of this study was to determine the antimicrobial susceptibility pattern of the brucella isolates.

Materials and Methods: From April to September 2014 a total of 30 brucella isolates that were cultured on brucella agar has been studied. The species identification was carried out and to determine the effect of antibiotics on bacteria antibiogram testing was performed by disk diffusion.

Results: In this study, 30 brucella strains were isolated from cultured specimens and antibiogram testing was performed. All microbial positive specimens were sequenced by PCR. All isolates were Brucella melitensis. According to the tests, suceptibility to tetracycline, minocycline, gentamicin, tigecyclin was 100%, to doxycycline 93.3%, co-amoxiclave 66.7%, rifampin 44.7%, streptomycin 86.7%, ciprofloxacin 80%, cotrimoxazole 76.7% and ceftriaxone 73.3%.

Conclusion: This study shows that the predominant strain in our patients was Brucella melitensis. Also, due to high levels of resistance to rifampin to use the other effective drugs like gentamicin, streptomycin, ciprofloxacin or cotrimoxazole in combination with doxycycline or tetracycline.