Journal of Arak University of Medical Sciences

Search published articles

Showing 5 results for Binding

Investigation of Mutations in Exons 15 and 18 of MYBPC3 Gene in Hypertrophic Cardiomyopathy Patients

Behnaz Sadat Abedi, Zohreh Kiyani, Shahrbanoo Parchami, Morteza Hashemzade Chaloshtari, Abbas Doosti,
Volume 18, Issue 5 (8-2015)

Abstract

Background: Hypertrophic cardiomyopathy (HCM) is a various collection of heart diseases with autosomal dominant inheritance affecting 0.2% of the global population. HCM is also the most common cause of sudden cardiac death in individuals younger than 35 years old. Approximately, 40% of affected cases are associated with MYBPC3 gene. The aim of this study is to investigate the possible presence of mutation in 15 and 18 exons of MYBPC3 gene in patients with HCM in Chaharmahal Va Bakhtiyari province.

Materials and Methods: In this experimental study, 30 HCM patients were selected. DNA was extracted using standard phenol-chloroform method. Certain exons were amplified by PCR method. And then, SSCP and HA methods were run.

Results: Significant differences were observed between the positive control samples and other samples. However, there were no difference in studied exons or shift in the bands.

Conclusion: Mutations in the exons of MYBPC3 gene may cause the HCM disease, and change in other exons may be the causative agent in this geographical region and change in this studied exons may not have contributed to the HCM disease. However, it is necessary to study more patients for getting a better conclusion.

The Relationship between Lymphocytic ABCA1 Protein with IL10 and TNF-α Cytokines Followed by one period Interval Combined Exercise Training in Overweight and Obese Male Adolescents

Bahloul Ghorbanian,
Volume 19, Issue 5 (8-2016)

Abstract

Background: The study on rats showed that some of cytokines and proteins which were produced by macrophages and other cells, plays a critical role in regulating of ABCA1 expression. But, in this area, the study in human subjects, especially subsequent physical activity has not performed. The purpose of this study was to investigate the relationship between lymphocytic ABCA1 protein with IL-10 and TNF-α cyrokines subsequent eight weeks interval combined exercise training (ICET) among overweight and obese boy adolescents.

Materials and Methods: In this semi-experimental study, 28 students (16.93±1.89 yr, 88.07±9.98 kg and 28.35±2.55 kg/m²) were randomly selected and assigned into training (n=13) and control (n=15) groups. Exercise protocol was ICET (8WK, 4 d/wk, 70 min/d).Cell hemolysis and sensitive Elisa method was used for evaluating ABAC1 protein T-student tests and Pearson correlation were used to analyze the data.

Results: The survey changes of post to pre-test of ABCA1, IL-10 and TNF-α showed that there was a positive significant correlation between lymphocytic ABCA1 protein with IL-10 (r=0.43, p=0.032) and a negative significant correlation with TNF-α (r=-0.53, p=0.012) (p<0.01) after eight weeks training. Also, after exercise, ABCA1 level was significantly increased but the levels of in creased IL10 and decreased TNF-α were not significant.

Conclusion: Due to the increased lymphocytic ABCA1 protein concentration and the correlation between variables following training, the results prove that TNF-α and IL-10 may have negative and positive regulatory effects on lymphocytic ABCA1 protein expression, respectively.

The Association of Genetic Variant rs8506C>T at miR-526b Binding Site in the LincRNA-NR_024015 Exon with Breast Cancer Risk

Fatemeh Tavakoli, Somayeh Reiisi,
Volume 23, Issue 2 (5-2020)

Abstract

Background and Aim: The long noncoding RNAs (lncRNAs) is an important type of RNAs that can regulate gene expression and, therefore, are involved in the development of various cancers. The genome-wide association study (GWAS) is used to identify phenotype-related loci within non-coding regions. However, the biological functions and exact relationships between phenotype-related loci and lncRNAs have not fully been identified. No study was found on the relationship between rs8506C>T polymorphisms in the lincRNA-NR_024015 exon and breast cancer susceptibility and clinical factors. Therefore, the present study aimed to evaluate the effect of polymorphism rs8506C>T on the breast cancer risk.
Methods & Materials: In this case-control study, participants were 120 patients with breast cancer, 120 healthy controls. The genetic variant was genotyped by using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR–RFLP) method. Interactions between the polymorphism and clinical factors were further evaluated, and Odds Ratio (OR) was measured for risk assessment.
Ethical Considerations: This study has been approved by the Research Ethics Committee at Shahrekord University of Medical Sciences (code:????) .
Results: There was a correlation between rs8506 C>T polymorphism and breast cancer risk in the dominant model (CC and CT+TT genotypes; P=0.027; OR=1.84; 95% CI: 1.067‐3.201). In the co-dominant model, CT genotype had a statistically significant association with breast cancer risk (P=0.038). Subjects with T allele in the rs8506 polymorphism had an increased risk of breast cancer (OR=1.69; 95% CI: 1.047-2.736; P=0.031). No relationship between rs8506 polymorphism and clinical factors including metastasis, tumor grade, and Human Epidermal Growth Factor Receptor 2 (HER2) status was observed.
Conclusion: Genetic variant rs8506 C>T polymorphism in the lincRNA-NR_024015 exon may contribute to the breast cancer risk. Allele T in this variant confers an increased risk of breast cancer. Further functional analyses are required to detect the detailed mechanism underlying the observed association.

Evaluation of the Method of Binding Tamoxifen to DNA Experimentally and Computationally

Fatemeh Sharafi Bajgan, Reza Safari, Maryam Nejat Dehkordi,
Volume 24, Issue 4 (9-2021)

Abstract

Background and Aim: Tamoxifen is a group of drugs of selective estrogen receptor modulators, and is one of the drugs effective in the prevention and treatment of some cancers (such as breast cancer). In this study, the interaction of tamoxifen with DNA is investigated experimentally. Also, the electronic structure (at atomic scale) of the molecular system of tamoxifen was theoretically investigated, using atom in molecule (AIM) theory.
Methods & Materials: First, in the experimental section of this study, the interaction of Tamoxifen with DNA were investigated by UV-ViS technique and hydrodynamic method (Viscometry). In addition, the analysis of the experimental results shows the obvious effect of concentration on the mechanism of how the tamoxifen molecule binds to DNA. Then, in the theoretical part of this research, using computational biophysical chemistry methods, some properties of tamoxifen molecular system, such as electronic Density of States (DOS), boundary orbital’s energy (HOMO/LUMO), Electrostatic Potential Energy (EPS) and electronic contour maps of the electron density and its Laplacian, will be calculated.
Ethical Considerations: This article is a meta-analysis with animal sample.
Results: Result of the UV-ViS spectroscopy technique and viscometry indicated hyperchromism and hypochromism effect. In addition, the result were depend on the concentration of the drug and affected the kind of binding of Tamoxifen to DNA. the analysis of computational studies on the drug tamoxifen suggests that the mechanism of the local charge/energy distribution in the molecular system of tamoxifen plays an important role in how this drug binds to DNA.
Conclusion: Based on the experimental results of UV-ViS technique and viscometry, as well as the electronic/vibrational properties of the tamoxifen molecular system, it was defined that the Tamoxifen interacts significantly with all the binding sites of DNA.

The Effect of Aerobic Training and Berberine Supplementation on the Expression of Dopamine 4 Receptor and CREB Gene in Heart Tissue of Female Rats During Withdrawal from Methamphetamine

Miss Zahra Mortezaei, Dr Somayeh Rajabi, Dr Sayyed-Javad Ziaolhagh,
Volume 26, Issue 3 (9-2023)

Abstract

Introduction: Methamphetamine increases the release of dopamine from nerve terminals. Binding of dopamine to dopamine receptors increases the phosphorylation of cyclic adenosine monophosphate responsive element (CREB) protein and changes the transcription of downstream genes.The purpose of this study is to investigate the effect of methamphetamine induction followed by aerobic training and Berberine on dopamine receptor 4 and CREB gene expression in the heart tissue of methamphetamine-addicted female rats during the withdrawal period.
Methods: 30 rats were randomly divided into 5 control groups, methamphetamine, methamphetamine + aerobic training, methamphetamine + Berberine, methamphetamine + aerobic training + Berberine. Intraperitoneal injection of 10 mg/kg of methamphetamine was performed for 5 days, and during the withdrawal period, aerobic training was performed for 4 weeks and simultaneously the consumption of berberine 100 mg/kg as a solution in drinking water was considered. Real Time PCR method was used to measure gene expression. Independent T-test, Kruskal-Wallis test and SPSS24 software were used at the level of 0.05 to analyze the data. The code of ethics in the research was received with number IR.IAU.SHAHROOD.REC.1402.015.
Results: The results showed that methamphetamine use caused a non-significant increase (97%) in CREB expression and a non-significant decrease (52%) in dopamine 4 receptor compared to the control group (P>0.05). The implementation of interventions during the withdrawal period, such as Berberine consumption and the combination of berberine with aerobic training, produced non-significant increasing and decreasing effects on dopamine 4 receptor gene expression and CREB in the heart of methamphetamine-addicted rats, respectively (P > 0.05).
Conclusions: Short-term induction of methamphetamine did not cause significant changes in the expression of dopamine 4 receptor and CREB genes in the heart. Therefore, these genes could not undergo a significant change as a result of interventions such as Berberine and exercise. More studies are needed to investigate exact genetic changes in heart tissue.

Page 1 from 1

Designed & Developed by : Yektaweb