Showing 18 results for Antioxidant
Neda Baghy Nia, Shahrbanoo Oryan, Ali Fani, Aliakbar Maleky Rad,
Volume 11, Issue 4 (12-2008)
Abstract
Background: Imbalance between the production of free radicals and the antioxidant defense system causes oxidative stress. The cardamom is the fruit of the Elettaria cardamomum maton which probably has an antioxidant property. Aim of this study is assess of effect of cardamom- tea watery extract on oxidative stress.
Methods and Materials : This clinical trial research was carried out on 54 persons. Firstly 5ml venous blood was collected and the parameter of the oxidative stress in their blood was evaluated. Then they were given every day 3 grams of cardamom and 1 gram of tea as like as 100 ml cardamom- tea watery extract for two weeks. After 2 weeks 5 ml blood was collected and the parameters of oxidative stress in their blood serum was evaluted for the second time. The parameters of first and second group of bloods were compared by SPSS software.
Results : The mean of total antioxidant capacity of serum before and after cardamom- tea extract were 1.96 ± 0.64 m mol/ml and 2.23 ± 0.46 m mol/ml respectively (p=0.009). The mean of lipid peroxidation before and after cardamom- tea were 14.97 ± 3.58 nmol/ml and 12.07 ± 5.91 nmol/ml respectively (p=0/002). The mean of total thiol group before and after cardamom- tea were 0.224 ± 0.200, 0.266 ± 0.166nmol respectively (p=0.141).
Conclusion: The cardamom- tea reduces the amount of lipid peroxidation and enhanced the antioxidant markedly. So, the cardamom- tea decreases the amount of oxidative stress in blood. Ther- fore the cardamom- tea in the people diet is usful.
Morteza Behnam Rassouli, Nargess Ghayour, Majid Afsharian, Maryam Tehranipour, Mohammad Bagher Ghayour,
Volume 13, Issue 1 (4-2010)
Abstract
Background: Oxidative stress is one of the probable molecular mechanisms involved in lead (Pb) neurotoxicity. On the other hand, lemon balm (Melissa officinalis) which is widely used in traditional medicine, has a high antioxidant activity. In this study, the protective impacts of Melissa officinalis on the adverse effects of Pb toxicity on learning ability were investigated. Materials and Methods: In this experimental-clinical trial, 40 virgin Wistar rats were mated and divided into control, control positive (vitamin C+Pb), control negative (Pb), and 3 Pb and Mellisa experimental groups. Mellisa was orally administered in three doses including + 25, 50 and 100 mg/ kg of body weight daily. Treatment started from 7th day of gestation and continued through pregnancy and lactation periods. The three month- old offsprings in each group were assessed in terms of memory and learning ability by Morris water maze test and the results were compared between the groups. Results: Exposure to Pb during and after gestation leads to learning disorders. While concomitant administration of Pb and Melissa, as well as vitamin C can, to a large degree, reduce the adverse effects of Pb on learning abilities. Conclusion: Since no significant differences were obtained from the comparison of results in the control, Pb+M and vitamin C+Pb groups, it can be concluded that Melissa has antioxidant impacts equal to vitamin C. Therefore, similar to vitamin C, Mellisa can decrease the neurotoxic effects of Pb.
Mahmood Amini, Seid Alireza Hosseini, Yahya Jand, Tooraj Zandbaf, Babak Eshrati, Shabanali Alizadeh, Ghasem Mosayebi, Ali Ghazavi, Ali Cyrus,
Volume 13, Issue 4 (1-2011)
Abstract
Background: Nowadays, the use of inflammatory biomarkers in the diagnosis of appendicitis is on the rise. On the other hand, the role of oxygen free radicals in various inflammatory states has been verified. Noticing the high prevalence of negative appendectomy (9.3-22.2%), the aim of this study was to investigate the level of blood total antioxidant capacity (TAC) as a biomarker for early diagnosis of acute appendicitis. Materials and Methods: In this prospective analytical trial, over a one-year period, 407 patients, with preoperative diagnosis of acute appendicitis who had undergone operation, were studied. After measuring the TAC level, data were analyzed through the analytic ROC curve and parametric mean comparison tests. Results: Of all the patients, 298 were identified with appendicitis. TAC levels in adult female and male groups were respectively 663.9±164.75 and 752.58±167.37 µm/L which revealed a significant difference (p<0.0001). Also, in children, the mean of TAC level in perforated appendicitis sub-group was significantly greater than those in gangrenous and suppurative groups. Conclusion: TAC level can be used as a predictive factor for the diagnosis of perforated appendicitis in children however, it cannot be used as a biomarker for the diagnosis of appendicitis. Because of the significant difference existing between the level of TAC in male and female patients, further studies are suggested to investigate the level of inflammatory biomarkers with respect to menstrual cycle in women.
Hadi Ansarihadipour, Maryamsadat Alhoseini, Soheila Rostami , Narges Farahani, Mahya Hashemi ,
Volume 15, Issue 2 (6-2012)
Abstract
Background: The aim of this study is to assess antioxidative and pro-oxidative efficacy of ascorbate on serum albumin during iron-induced oxidative stress. Materials and Methods: In this experimental study, albumin was placed in the oxidative system containing iron ions and different concentrations of ascorbate. To monitor albumin degradation, sodium dodecyl sulfate polyacrylamide gel electrophoresis was performed according to Laemmli procedure. Oxidative modification of albumin was demonstrated using a method for determination of carbonyl groups by 2,4-dinitrophenylhydrazine. Results: By applying the carbonyl assay, ascorbate showed a dual effect: initial pro-oxidative effect on albumin changed to an antioxidant one in a dose-dependent manner. Our findings showed prooxidant effects for ascorbate in low concentrations (0-100 µM) and antioxidant effects in higher concentrations (100-300 µM). Also, electrophoretic pattern of plasma proteins showed significant protein aggregations in the range of 35 to 45 kDa of MW and protein degradations in the range of 115 to 180 kDa. Conclusion: Ascorbate can produce reactive oxygen species and can also inhibit the production of these oxidants in the presence of iron ions as well. These findings may be directly applicable to oxidative states during the administration of ascorbate and may be important in preventing oxidative modifications of proteins in blood circulation and other biological fluids.
Siavash Fazelian, Ahmad Saedi Someeh Olia, Mrayam Mirftahi, Mostafa Hoseini, Haleh Sadrzade Yegane, Javad Heshmati, Nazli Namazi,
Volume 16, Issue 9 (12-2013)
Abstract
Background: Considering that pre-diabetes is one of disorders that increases developing of diabetes, this study was performed to investigate the effect of L-Arginine supplementation on antioxidant enzyme activity, total antioxidant capacity, and body composition in patients with pre-diabetes.
Materials and Methods: A double-blinded randomized clinical trial was performed on 46 pre-diabetic patients. Patients in intervention (n=23) and placebo groups received 3g/day L-arginine and placebo (three 1gr L-arginine capsules), respectively for 8 weeks. Anthropometric, body composition, and dietary intake assessments, and also biochemical tests were done at baseline and end of study. Total Antioxidant Capacity (TAC), glutathione peroxidase enzyme and superoxide dismutase activity were measured. Paired t-test, independent t-test and ANOVA were used to analyze quantitative variables.
Results: Thirty eight patients (19 subjects in each group) completed the study. After adjusting confounding factors, no significant difference in body composition, anthropometric indices and dietary intake was observed at the end of study (p<0.05 in all factors), but a significant difference in TAC level was observed in the intervention group at the end of study (p<0.001).
Conclusion: Supplemenation with 3gr/day L-arginine for 8 weeks led to raised serum TAC level without changes in body composition in pre-diabetic patients.
Ali Akbar Malekirad, Mohammad Abdollahi, Mohammad Fazilati, Najme Igani, Mahmod Reza Baghinia, Azam Taheri, Mohammad Amin Rezvanfar,
Volume 17, Issue 1 (4-2014)
Abstract
Introduction: Paints are composed of extensive variety of hazardous substances, such as organic solvents and heavy metals. Occupational exposure to paint increases oxidative stress, hematological disorders, and DNA injury. This study was performed to explore the beneficial effects of Selvit on the paint workers by measuring total antioxidant capacity, lipid peroxidation, and blood parameters as main oxidative stress biomarkers.
Materials and Methods: This clinical trial study was conducted on 25 male workers who worked in the paint factory. Serum markers such as total antioxidant capacity (TAC), lipid peroxidation (LPO) and also hematological parameters were measured before and after using Selvit (14 days). SPSS software was used for data analyses and paired t-test and Pearson correlation analysis were applied.
Results: Results showed that after using the Selvit, an increase in TAC and a decrease in LPO were observed but they were not significant. Also, aspartate transaninase significantly decreased after using Selvit and high density lipoprotein significantly increased.
Conclusion: Selvit have beneficial effect on hematological parameters due to its antioxidant capacity.
Gholam Reza Shahsavari, Asghar Mohammad Pour Konani, Arezou Miraftabi,
Volume 18, Issue 2 (5-2015)
Abstract
Background: Oxidative stress and antioxidant status may be associated with glaucomatous damage. The purpose of this study was to compare the serum antioxidant status between primary angle closure glaucoma patients and healthy subjects.
Materials and Methods: In this case-control study 66 primary angle closure glaucoma patients and 80 controls were enrolled. Peripheral blood sample obtained from patients. Superoxide dismutase(SOD) was assayed by inhibition the rate of adrenochrome formation. Catalase (CAT) was evaluated by decrease of H2O2 absorbance. Glutathione peroxidase (GPx) and Glutathione reductase (GR) were determined following NADP oxidation or reduction. Glutathione S-transferase (GST) was measured by increase in the absorbance of CDNB and glutathione conjugation.
Results: Antioxidant defense enzymes were significantly decreased in glaucoma patients over those of control groups. CAT (p<0.006), SOD (p<0.020) and GPX (p<0.004). A relative insignificant decrease of GR and GST activities was observed in glaucoma patients compared with healthy subjects.
Conclusion: The present study supports the hypothesis that oxidative stress is an important factor in the pathogenesis of glaucoma. Although primary angle closure glaucoma has an anathomical basis but decrease in antioxidant defense enzymes activities may have a role in pathogenesis of this type of glaucoma.
Somayeh Jahani, Masoud Salehi, Amin Shakiba, Aliasghar Moradipour, Forouzan Forouzandeh,
Volume 18, Issue 5 (8-2015)
Abstract
Background: Ferula assa-foetida is one of the common medicines that was used as antiseptic with a view to traditional uses, it can be used as a safe and effective drug to treat diseases particularly resistant bacterial infections. This study aims to product gelatin nano- capsules containing Ferula assa-foetida essential oil and investigate their antioxidant and antibacterial activities.
Materials and Methods: This experimental study was performed on gelatin nano-capsules containing Ferula assa-foetida essential oil (FAO) (2, 4, 6 and 8% w/w), glycerol (25% w/w) as plasticizer and glutaraldehyde as cross-linker. The morphology, antioxidant and antibacterial activities and operation of the nano-capsules were assessed according to American Standards by Scanning Electron Microscopy, ABTS, and microbiological tests.
Results: Gelatin nano-capsules exhibited low antioxidant and antibacterial activities while gelatin nano-capsules incorporated with FAO exhibited excellent antioxidant and antibacterial. The highest rates of these effects were seen merged with 8% of FAO.
Conclusion: Gelatin nano-capsules merged with FAO have excellent physical form, as well as they are an appropriate antioxidant and antibacterial that have been considered to produce antoxidant and antibacterial drugs.
Majid Askaripour, Syed Reza Fatemi Tabatabaei, Hossein Najafzadehvarzi, Foruzan Hosseini,
Volume 18, Issue 11 (2-2016)
Abstract
Background: Renal injury following ischemia - reperfusion (I/R) is still an unavoidable problem in many remedial and medical situations. Portulaca oleracea (PO) has been known for its anti-oxidative effects. Then, the present study aimed to investigate the effect of ethanolic extract of PO (EEPO) on the renal function and antioxidant status after induction of I/R injury in the rat kidney.
Materials and Methods: A total of 30 rats (Wistar) were divided into five groups (n = 6 each). Sham group: underwent laparotomy without I/R, EEPO group: EEPO administered 300 mg/kg then was operated like sham, I/R group: was underwent renal ischemia/reperfusion only, EEPO150+ I/R and AEPO300+ I/R groups: were administered PO 150 and 300 mg/kg then underwent I/R operation. PO extract was administered for 5 days in the relevant groups by gavage. Serum urea and creatinine (Scr), the level of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and total antioxidant activity (TAA) were determined. Data were analyzed by ANOVA and post hoc LSD test. P values of 0.05 or less were considered statistically significant.
Results: Induction of I/R and pretreatment with PO extract, increased the level of superoxide dismutase (SOD) in comparison with sham group (p<0.05, p<0.001). There were no significant differences in the levels of MDA, GSH and TAA among different groups. On the other hand, the Scr and serum urea of the I/R and treated groups were elevated compared to the sham group (p<0.001).
Conclusion: Ethanolic extract of PO did not strongly affect the renal antioxidant status and could not prevent the renal injury following I/R.
Mohsen Ebrahimi Monfared, Mehdi Sadegh, Zahra Gohari,
Volume 19, Issue 12 (3-2017)
Abstract
Abstract
Background: Melatonin regulates the sleep-wake cycle and is protected against oxidants. Vitamin E has antioxidant effect. The aim of this study was to investigate effect of melatonin and/or vitamin E on the EEG, sleep quality and quality of life of female nurses working in hospitals in Arak.
Materials and Methods: 60 female nurses were randomly selected from hospitals in the Arak city. Participants divided into three groups (melatonin3 mg/day, vitamin E 200 IU/day and melatonin and vitamin E groups) and a control group. Before and two months after the baseline, PSQTI and the quality of life of 26 questions questionnaire were completed and EEG was recorded. Finally, the findings for both groups before and after the intervention were compared with each other.
Results: The average of sleep quality showed an improvement in groups receiving melatonin and melatonin+vitamin E (p>0.05). While data of life quality did not show any significant change between groups. In EEG activity, α waves in the range of 8-10 Hz showed maximum increase in melatonin group and minimum increase in vitamin E group. Also, α wave in the range of 10-12 Hz revealed the most decrease in the melatonin group.
Conclusion: It seems simultaneous administration of melatonin and vitamin E is able to improve sleep quality. In addition, melatonin could improve alpha-waves of brain activity.
Farzaneh Rooshenas, Mahboobeh Ashrafi, Saeed Nazifi, Mahmoud Aminlari, Sara Talebanzadeh,
Volume 21, Issue 5 (10-2018)
Abstract
Background and Aim: Medicinal plants possessing antioxidant activity may reduce oxidative stress and improve the functions of various organs that affected by hyperglycemia. The aim of the present study was to evaluate the effects of saffron aqueous extract (SAE) administration to diabetic rats by measuring the oxidative stress parameters and important biochemical enzymes in liver tissue.
Materials and Methods: 72 hours after STZ administration (60 mg/kg body weight), the animals with fasting blood glucose over of 250 mg/dl were considered to be diabetic rats and experimental groups were: control (1), control drug (2), diabetes (3) and diabetes drug (4). The treatment was started on the 7th day after STZ injection with i.p injection of SAE (200mg/kg body weight), five doses and weekly to groups 2 and 4. At the end of the experimental period, biochemical factors were measured after bleeding and harvesting of tissues.
Findings: Results indicated the perturbation in the activity of important liver enzymes in diabetic group (3) and SAE adjusted and normalized their levels activity. In addition, SAE with increases in the activity of antioxidant enzymes alleviated diabetes induced oxidative stress and thus reduced MDA levels in group 4 compared to group 3.
Conclusion: SAE is not only useful in the controlling of blood glucose, but also has antioxidant potential to protect the liver tissue of diabetic rats against damage caused by hyperglycemia-induced oxidative stress.
Zohre Sadat Tabatabayi, Masoud Homayouni Tabrizi, Ali Neamati,
Volume 21, Issue 7 (2-2019)
Abstract
Background and Aim: Increasing metabolism and production of free radicals in the body are among the factors causing increased oxidative stress, weakened the antioxidant system, and some diseases, including cancer. The purpose of this study was to investigate the antioxidant activity and toxic effect of the Schiff base manganese II compound N, Nʹ di pirodexil 1, 4 butadiamin.
Materials and Methods: The antioxidant capacity of the Schiff base manganese II complex N, Nʹ di pirodexil 1, 4 butadiamin at the concentrations of 62.5, 125, 250, and 500 µg/ml was Investigated using the 1.1-diphenyl-2-picricyl-hydrazyl (DPPH) technique. We also evaluated the toxic effect of the mentioned Schiff bases complex at concentrations of 0, 62.5, 125, 250 and 500 µg/ml by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) at 24, 48 and 72 hours on a breast cancer cell line (MCF7 [Michigan Cancer Foundation-7]).
Ethical Considerations: In this study, research ethical principles were considered.
Findings: The antioxidant capacity of Schiff bases manganese II complex N, N di pirodexil 1, 4 butadiamin at the concentration of 500 µg/ml was 38.83%. Further, considering the toxic effect of the Schiff bases on the MCF7 cell line, the results showed that IC50 at 24, 48 and 72 hours was about 124, 245 and 470 µg/ml, respectively.
Conclusion: The results obtained from the review of the antioxidant capacity of the Schiff base manganese II complex N, Nʹ di pirodexil 1, 4 butadiamin compared to (BHA) butylated hydroxyanisole suggest that the compound can be effective in free radical inhibition.
Fatemeh Samimi, Maryam Baazm, Ebrahim Eftekhar, Farideh Jalali Mashayekh,
Volume 22, Issue 4 (9-2019)
Abstract
Background and Aim Oxidative stress is the main factor in the development and progression of diabetes and its related complications. There is growing evidence that antioxidants supplementation can improve oxidative stress induced in diabetes. The present investigation was conducted to study the effects of Coenzyme Q10 (CoQ10) on the Oxidative Stress Index (OSI) in diabetic rats.
Methods and Materials A total of 30 male rats were divided into five groups: saline, sesame oil (as a vehicle), CoQ10-treated (10 mg/kg/day), diabetic (induced with streptozotocin: 55 mg/kg), and CoQ10-treated diabetic (10 mg/kg/d). Then, we measured the Malondialdehyde (MDA), Total Oxidant Status (TOS), and Total Antioxidant Capacity (TAC) levels in the rats’ liver homogenate. Additionally, the OSI was calculated.
Ethical Considerations The Research Ethics Committee of Arak University of Medical Sciences approved this study (Code: IR.ARAKMU.REC.1397.119).
Results results showed a significant decrease in the level of liver MDA (P=0.022), TOS (P=0.03), and OSI (P=0.028) in the CoQ10-treated diabetic group compared to the diabetic rats. No significant change was observed in the total thiol group (P=0.25) and TAC (P=0.77) level in diabetic rats’ livers treated with CoQ10 compared to diabetic rats.
Haleh Sadat Tavakkol Afshari, Masoud Homayouni Tabrizi, Touran Ardalan,
Volume 22, Issue 4 (9-2019)
Abstract
Background and Aim Among nano-pharmaceutical materials, nanoemulsions are an important tool in the field of nanotechnology. They have been designed for clinical and therapeutic applications. Since the therapeutic efficacy of cancer is measured by the ability of the drug to reduce and eliminate tumors without damaging healthy tissues, nanoemulsions can be useful as a targeted drug carrier. Therefore, we are going to study the antioxidant and anticancer effects of nanoemulsions prepared using dill essential oil.
Methods and Materials Evaluate the antioxidant properties, we used DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2'-azino-bis[3-ethylbenzothiazoline-6-sulphonic acid]) tests. We also applied MTT assay for the evaluation of induced cytotoxicity in liver hepatocellular carcinoma cells and normal human umbilical vein endothelial cells.
Ethical Considerations The Research Ethics Committee of Islamic Azad University (Mashhad Branch) approved this study (Code: IR.IAU.MSHD.REC.1398.027).
Results Nanoemulsions prepared using dill essential oil has a good potential for inhibiting DPPH (IC50=500 μg/mL) and ABTS (IC50=420 μg/mL) radicals. Nanoemulsions also caused a little toxicity to both cell lines. Nanoemulsions reduced cell viability in a dose-dependent manner and the cytotoxicity induced to cancer cells was higher than normal cells.
Conclusion The present study indicates that nanoemulsions prepared by dill essential oil will have the potential to become a therapeutic strategy for diseases caused by oxidative stress.
Sahar Dehghani, Leila Rouhi, Noosha Ziya Jahromi, Reza Dehghani, Khalil Khashei Varnamkhasti,
Volume 24, Issue 2 (5-2021)
Abstract
Background and Aim: Proliferate potential differentiate into different cell lineages and high self-renewal of Mesenchymal Stem Cells (MSCs); thus, they are ideal tools for regenerative medicine. However, a leading problem is an oxidative stress in the target tissue and the apoptosis of transplanted stem cells before tissue repair. The pretreatment of stem cells with antioxidants may make them resistant to oxidative stress. Ginger is the main medicinal plant with antioxidant properties. This study explored the antioxidant effects of ginger extract on bioavailability and oxidative stress-induced apoptosis in human adipose tissue-derived mesenchymal stem cells and rat bone marrow examined.
Methods & Materials: In this study, human adipose tissue-derived mesenchymal stem cells and rat bone marrow were cultured in a DMEM medium with 20% FBS. The explored cells were incubated for 4 and 6 hours for pretreatment with different concentrations of ginger extract (50, 100, 200, & 400 mg/mL); then, they were treated with 200 μM H2O2 for 2 hours. Bioavailability was analyzed by ELISA reader using an MTS kit and apoptosis was analyzed by flow cytometry using an Annexin V-FITC/PI kit into the manufacturer’s protocol at both times. The obtained data were analyzed by Analysis of Variance (ANOVA) using SPSS.
Ethical Considerations: This study was approved by the Ethics Research Committee of Shahrekord Branch, Islamic Azad University (Code: IR.IAU.SHK.REC.1397.028).
Results: The MTS results indicated a dose- and time-dependent manner increase in the bioavailability of human adipose tissue-derived mesenchymal treated stem cells. Ginger extract treatment also dose- and time-dependently decreased the rate of apoptosis in rat bone marrow mesenchymal stem cells.
Conclusion: Ginger extract, by reducing the oxidative stress in mesenchymal stem cells, elevates their lifespan in the target tissue, and increases the efficiency of these cells in tissue regeneration.
Zahra Bahramnezhad, Ali Ghazavi, Ali Ganji, Ghasem Mosayebi,
Volume 24, Issue 3 (8-2021)
Abstract
Background and Aim: Medicinal plants contain various biological compounds, including phenols, flavonoids, and terpenoids, with anti-radical and anti-inflammatory activities that affect human health and improve life. Considering the numerous beneficial effects of Tanacetum Parthenium and Satureja Montana and the lack of full antioxidant capacity and anti-inflammatory effects of their combination, we decided to combine these two plants’ anti-inflammatory and antioxidant effects on induced acute inflammation in BALB/c mice.
Methods & Materials: In the present study, mice received the extracts of T. Parthenium and S. Montana and their combination by gavage for 14 consecutive days. Then, to induce acute inflammation, thioglycollate was injected intraperitoneally to all groups. FRAP and Grease tests were used to evaluate the total antioxidant capacity and serum nitric oxide concentration, respectively. One-way ANOVA analyzed the results.
Ethical Considerations: This study was approved by the Research Ethics Committee of Arak University of Medical Sciences (Code: REC.1398.021).
Results: The results showed that the combination of the extracts (P=0.006) and Satureja Montana (P=0.021) led to a significant increase in total antioxidant activity compared to the control group. Also, according to the results of grease test, Satureja montana (P=0.04), Tanacetum parthenium (P=0.034), and their combination (P=0.003) significantly reduced serum nitric oxide production compared to the control group.
Conclusion: The present study shows the synergistic effect of the combined extracts to increase their total antioxidant capacity and anti-inflammatory.
Maryam Bahrami, Ali Ghazavi, Ali Ganji, Ghasem Mosayebi,
Volume 24, Issue 3 (8-2021)
Abstract
Background and Aim: In addition to free radicals such as Nitric Oxide (NO), inflammation is one of the most important pathophysiological causes of peritonitis. Over thousands of years, Nigella Sativa (NS) and Silybum Marianum (SM) are two plants known for their anti-oxidant and anti-inflammatory properties. However, the effect of its compound is unclear. Thus, in this study, we evaluated the anti-inflammatory effect of NS and SM extracts and their combination on inflammatory diseases like thioglycollate peritoneal.
Methods & Materials: Alcoholic extracts of SM and NS were obtained by the soxhlet method. Male Balb/C mice were divided into 5 groups and gavage orally for 14 days with SM, NS, the mixture of extracts of these two, DMSO 30% as the control group, and dexamethasone as the positive control group. The safety profile and acute toxicity in mice were assessed. On day 10, acute peritonitis was induced by thioglycollate 3%. Finally, the total anti-oxidant power and NO concentration were measured by FRAP and Griess method, respectively, in the serum of treated mice.
Ethical Considerations: All experimental process was performed following the guidelines according to the Animal Ethics Committee of Arak University of Medical Sciences (IR.ARAKMU.REC.1397.359).
Results: Acute toxicity test showed no significant changes in weight and physical appearance of the mice. However, the extract and their mixture decreased NO level significantly (P=0.000) in serum. Also, the mixture significantly increased total anti-oxidant power (P=0.015).
Conclusion: Results showed that the SM and NS extract mixture demonstrated anti-inflammatory activity, inhibiting inflammatory mediators such as NO and increasing anti-oxidant power, thus supporting its therapeutic potential in slowing down inflammatory processes in inflammation disorders.
Zahra Sadat Mousavi, Fatemeh Nasernakhaei,
Volume 27, Issue 6 (1-2025)
Abstract
Introduction: Bird′s-foot trefoil (Lotus corniculatus L., Fabaceae) has many medicinal properties due to its valuable chemical compounds. This research reviews the chemical compounds and medicinal properties of this plant.
Methods: This study used library resources, search engines, and databases such as Google, Google Scholar, PubMed, PubChem, ScienceDirect, Magiran, ResearchGate, and SID. The data were collected and classified.
Results: The results indicate that bird′s-foot trefoil is rich in valuable compounds such as kaempferol, quercetin, isorhamnetin, oleamide, and linoleamide. Different extracts of this plant have antioxidant, anti-inflammatory, immunostimulant, anti-cancer, anti-pain, anti-depressant, anti-amylase, anti-fungal, antiprotozoal, and anti-bacterial activities. It is used for wound healing, controlling anxiety and nausea, treating urinary infections, removing kidney stones, and as an antispasmodic and anti-hemorrhoid. Topical application of Lotus corniculatus seed extract has reduced the sebum contents and areas of oily human skin.
Conclusions: L. corniculatus can directly and indirectly affect human life and health. Due to the effective compounds and medicinal properties of Bird′s-foot trefoil, additional studies and clinical tests on its medicinal properties should be performed.
