Saeed Babaei, Saeed Changizi Ashtiani, Hamid Gerami, Ali Reza Jamshidifard , Mohammad Rafiee,
Volume 6, Issue 3 (10-2003)
Abstract
Introduction: At present, bone graft is unavoidable. In fact although among the all probable bone grafts, the autograft is the best choice, but because of some situations like two coincidence surgery upon one individual, size limitation of autografts (its impossible to get a big piece of graft from patient) increasing the bloodshed contingency, infection etc, made specialist to think about the possible substitution the allograft.
Materials and Methods: Seventeen Newzealandian rabbits were divided into four groups. The graft generator group consisted of one rabbit which was genetically different from other groups. The sham group composed of three rabbits which round autografts about 4 mm in diameter from their tibia were exchanged between rights and left tibias. These grafts were not deantigenized and a 4 mm vacant hole was created to see if it will repair spontaneously within three months. The control group consisted of three rabbits received a round 4 mm tibial autografts which double deantigenization process were done over them. The test group composed of 10 rabbits, received corticocancellous (intramembranous) allografts in left tibia and corticocancellous (endocondral). Allograft in right tibia all grafts were bearing the double deantigenization process.
Results: The rate of prosperity for CCl grafts was 80% and for CCE grafts was 60%.In sham group, healing process was extremely fast (in eight weeks).
In control group, healing process was done slower until six weeks but after 12 weeks it was compensated. The vacant holes were not repaired even after three months. Statistically, there was a meaningless difference between the rates of prosperity in both groups of grafts.
Conclusion: According to statistical assays, although the prosperous numbers of CCl were more than CCE grafts but this difference was meaningless. In the basis of histological assessments if the velocity of healing process is important, the CCI grafts are recommended
Maryam Jafarpour, Mojtaba Bayani, Leila Hesami-Moghadam, Ali Pooladi,
Volume 25, Issue 1 (3-2022)
Abstract
Background and Aim The use of allograft alone or in combination with other bone materials has significantly improved bone-augmentation procedures. Therefore, this study aimed to evaluate the effect of topical gentamicin solution (80 mg) with allograft on guided bone regeneration in defects in rat skull.
Methods & Materials Twelve Wistar mice were randomly divided into two groups, with 6 in each group. Three defects were created in their skulls, one filled with demineralized freeze-dried bone (DFDBA) allograft alone, the other with allograft with gentamicin 80 mg solution, and the third defect were left empty as a control group. After 4 and 10 weeks, the mice were killed for histological specimens. Histological and histomorphometric evaluations were performed to qualitatively and quantitatively assess bone formation, a new bone type made, new bone vitality, inflammatory response, connective tissue type, and angiogenesis.
Ethical Considerations This study was approved by the ethics committee of Arak University of Medical Sciences (Code: IR.REC.ARAKMU.1397.30).
Results At the end of 4 and 10 weeks after surgery, the average percentage of ossification in the area of defects was higher in the group that received allografts with gentamicin, and during this period, a significant difference was seen between these groups and the control group.
Conclusion According to the present study, the use of gentamicin in combination with DFDBA has a significant effect on bone regeneration in defects in the rat skull.
