Abdolrahman Dezfulian, Hayat Mombini, Shahla Zahiri, Farzaneh Dehgani , Abdolkarim Mansuri ,
Volume 5, Issue 4 (12-2002)
Abstract
Introduction: Cisplatin is a drug widely used as an antineoplastic drug for treatment of malignant tumors. But because of its side effects on the different systems especially kidney (nephrotoxic), the use of this drug is very limited. Clinical as well as, laboratory animal studies have supported this observations. In this research study we have used stereological technique (3-D) for finding the changes, due to nephrotoxic effect of this drug, in the number of glomeruli in kidney (numerical density and total number).
Materials and Methods: For experimental, 30 rats were separated by random sampling in to 3 groups of 10 animals cache. The first group received acute dose (7.5 mg/kg) of the drug (cisplatin) in serum physiology (experimental group). The second group received equivalent placebo dose in serum physiology through peritoneum (control). The third group received chronic dose (1.25 mg/kg) for 5 days, in serum physiology. All the 30 animals, after 96 hours, were anesthetized, dissected and their right kidneys were removed and placed in fixative (10% formalin). Whole kidney specimens were processed for stereology by special method of sectioning for physical disector and glomeruli number were counted.
Results: Number of glumeroli and numerical density was estimated for experimental groups (control, acute and chronic) was 31707, 30415 and 30802 as well 162, 119, and 140 respectively.
Conclusion: Stereological methods could be very useful for investigation of chemical drug effects in organs with good validity.
Ali Reza Susan Abadi , Mansour Parvaresh , Keyvan Ghasaami, Mitra Sadat Rezaei ,
Volume 5, Issue 4 (12-2002)
Abstract
Introduction: Diabetic neuropathy is a common complication of diabetes, leading to great morbidity and mortality and resulting in a huge economic burden for care of the patient with diabetes. It is the most common of neuropathy in the developed countries of the world, accounts for more hospitalizations than all the other diabetic complications combined, and is responsible for 30% 1075% of nontraumatic amputations.
Materials and Methods: This investigation is a descriptive analytic, cross-sectional study among 40 diabetic patients without symptoms of peripheral neuropathy referred to Aruk Vali-Asr diabetes clinic from March to september of 2003. Patients were referred to one neurologist for study of peroncal and tibial motor nerves and sural sensory nerve conduction velocity in electrodiagnostic clinic of Arak Amir Kabir hospital. The results of this group were compared to the results of 70 age and Gender matched normal control subjects. Independent samples T-Test, Pearson correlation coefficient and Fisher's exact test were used in analysis.
Results: A total of 40 patients were studied with mean age of 42.6 years and mean duration after the first diagnosis of diabetes 5.72 years. Mean investigated nerves conduction velocity in the normal control group was meaningful more than in diabetic patients. Asymptomatic neuropathy was present in 27 (67.5%) of patients. In these patients mean duration after the first diagnosis of diabetes was 6.5±5.16 years.
Conclusion: Asymptomatic diabetic neuropathy is common (67.5%) sural sensory. Nerve conduction velocity is a sensitive test for early diagnosis of diabetic neuropathy.
Mohammad Mahdi Karimi, Amir Majlesi,
Volume 5, Issue 4 (12-2002)
Abstract
Introduction: Infection due to hepatitis B virus (HBV) could be due to wild or mutant viruses. The HBeAg negative chronic hepatitis B (HbeAg-CHB) is unable to produce hepatitis B, e antigen (HbeAg), so that patients with this varient do not present with HBV characterized by HbcAg in the serum. HbeAg-CHB usually proceeds to serious liver disease. The prevalence of different viral forms in patients with chronic liver disease in Iran has not been established.
Materials and Methods: Seventy-six Hamadanian patients with over 6 months HBSAg positivity were enrolled. Patients with co-infection of HIV, HCV, past history of alcoholism, fatty liver, using of hepatotoxic drugs, autoimmune hepatitis and other metabolic liver disease were excluded. All patients were screened for Hhe Ag. HbeAb. HBV DNA by PCR, AST, ALT, ALK. pH and bilirubin.
Results: Eleven (14.5%) patients had HheAg-CHB (HbsAg +ve, HbeAg-ve / ve/anti HbeAb + ve, HBV DNA + ve and elevated AST, ALT) and 6 (8%) had normal transminases (AST and ALT) accompanied by the remaining criteria of HbeAg-CHB. 59 patients (77.5%) were infected with wild type HBV, ie: HBSAg + ve, HbeAg-ve, HbeAb + ve, HBV DNA- ve, and normal AST, ALT.
Conclusion: Frequency of HbeAg.CHB in Hamadan is 14.5% knowing of this varient of chronic hepatitis B is important since the HbeAg-CHB have worse prognosis than wild type and more better response to lamivudine than interferon.
Bahman Salehi, Morteza Pirhadi,
Volume 5, Issue 4 (12-2002)
Abstract
Introduction: Depression is one of the psychiatric disorders that causes loss of energy, decrease of concentration and daily activity that these also became decreased in motivation and educative activity of students. The aim of this study was assesed to determine the prevalence of depression and it's related factors of Arak university of medical sciences in 79-80.
Materials and Methods: This study was cross-sectional and the sample of study consisted of all medical sciences students that came to university on fall of 79 (N=153) that were screened for depression at the biginning time of educational course and then 3 terms later by using personal data questionair and short form (21 statements) of Beck Depression Inventory Test.
Results: From 153 students, 38 (%22.1) respondants at biginning of course obtained the score in between 17 and>17 were determined as depressive case, then after 3 terms this figure increased to 58 (%38.5), this differences was significant differences between depressive and nondepressive groups in variables such as proper of dornitory condition 3, satisfaction of field of study 3.64, satisfaction with the educative conditions of the university 3.49, satisfaction with student-management relationship 3.77, appropriate cmotional-supportative and security condition in between family and students 4.32, economical condition of safe and families 3.58, at biginning time and then 3 terms later in arrangement of above were 2.82, 3.66, 3.37, 2.79, 2.66, 4.17 and 3.67 that these diffrences due to constant samples were significant and has had significant roles in prevalence of depression.
Conclusion: This study showed that depression was increased in students of Arak University of Medical Sciences after 3 terms and it is related with some stressful factors such as family, social and economical factors.
Amir Almasi-Hashiani, Soheila Zareifar, Seyed Hamid Hosseini, Aziz Dehghan,
Volume 15, Issue 2 (6-2012)
Abstract
Background: Leukemia is the most common type of cancer in children which its relapse decreases the patients’ survival rate. The aim of this study was to determine the risk factors involved in leukemia relapse in patients in Shahid Faghihi Hospital, Shiraz, during 2004-2009 years.
Materials and Methods: In this retrospective cohort study, 280 patients with acute lymphoblastic leukemia and acute myeloid leukemia were included. Patient characteristics were analyzed with respect to their association with recurrence through Chi-square test, Fisher’s exact test, and logistic regression model using SPSS software version 16 (P-value<0.05).
Results: Logistic regression model revealed a statistically significance relationship between age and recurrence of the disease (odds ratio (OR) = 0.35, 95% confidence interval (CI) = 0.15-0.82), odds ratio of relapse in the 5-10 years old age group was 0.35 times more than the 0-5 years old age group (p=0.01).
Conclusion: Noticing the greater likelihood of relapse in 0-5 years old age group compared with the 5-10 years old age group, more attention and better follow-up for decreasing the side effects of the disease and enhancing the survival rate of the 0-5 y/o age group are recommended.
Davood Bashash, Seyed H. Ghaffari, Maryam Kazerani, Kebria Hezaveh, Kamran Alimoghaddam, Ardeshir Ghavamzadeh,
Volume 15, Issue 9 (2-2013)
Abstract
Background: Since nearly 90% of patients with acute promyelocytic leukemia (APL) have high telomerase activity and significant shortened telomere length, these patients have, therefore, been suggested to be good candidates for the therapeutic intervention with telomerase inhibitors. This study was done to investigate the effects of BIBR1532, a non-nucleoside inhibitor of telomerase, on APL cells. Materials and Methods: In this experimental study, for investigating the effect of BIBR1532, NB4 leukemic cells were cultured in the presence of various concentrations of BIBR1532. Succeeding apoptosis assay, Caspase-3 activity assay, and quantitative real-time PCR were applied to examine the effect of this drug on apoptosis percenage, enzymatic activity of Caspase-3, and quantitative expression of genes mRNA involved in apoptosis. Results: The results showed that BIBR1532 induced apoptosis in NB4 cells in a dose-dependent maner. Moreover, real time PCR results showed that BIBR1532 led to a significant decrease in mRNA of Bcl-2 gene and signficant increases in transcription of Bax, PUMA, and Caspase-3. Conclusion: Since treatment with BIBR1532 could exert rapid apoptotic cell death in NB4 cells andactivate cellular apoptosis route, anti-telomerase-based therapy can regarded as a suitable strategy for APL treatment. Patients with progressive shortening of telomere length and high levels of telomerase activity are suitable candidates for treatment with telomerase inhibitors.
Mojtaba Sharafkhah, Ghasem Mosayebi,
Volume 16, Issue 2 (5-2013)
Abstract
Background: Epidemiologic studies show the high prevalence of some infections and cancers in individual blood groups. Perhaps, level of immunity factors differs in people with individual blood group. The aim of this study is to compare the level of antibodies and complement factors in ABO blood groups.
Materials and Methods: In this descriptive study, peripheral blood samples from 40 male healthy individuals with different ABO blood groups (n=10 in each group) with similar age (18-25 years) and genetic background were collected. The serum levels of total IgG, IgM, IgA, C3, and C4 were analyzed by nephelometry method. All data were analyzed by SPSS software version 11.5.
Results: The levels of IgA, IgG, and C4 protein in individuals with O blood group were significantly higher than those of other groups (p=0.02, p=0.01, and p=0.004, respectively). Also, the mean concentration of IgM in individuals with AB blood group was significantly higher than that in other groups (p=0.02). There was a significant difference between the level of C3 in O blood group and those of other blood groups (p=0.01). The mean concentrations of all parameters (except IgG) in B blood group were lower than those in other blood group.
Conclusion: Generally, natural antibodies in O blood group were higher than those in other groups. This high level of total antibodies in O blood group may reduce susceptibility to some infections.
Masoumeh Heidari Bateni, Shabanali Alizade, Akbar Hashemi Tayer, Amir Almasi Hashyani,
Volume 16, Issue 3 (6-2013)
Abstract
Background: Platelet glue is a human plasma product that is rich in platelets growth factors and fibrinogen and has haemostatic and healing properties. In the present study, the effect of fibrin sealant rich in platelet growth factors on healing burn wounds was evaluated.
Materials and Methods: This randomized clinical trial was done on 50 burn patients divided into two equal groups and followed for one month. Both groups received antibiotics during the treatment. Granulation tissue formation and epithelialization were the tokens of wound recovery and positive response to treatment. Data analysis was done by Fisher’s exact test, Mann-Whitney test, and t-test using SPSS software version 16.
Results: The mean of time to complete healing was significantly different in intervention and control groups (p=0.001). Time to recover 100% was 11.5±2.2 days in the intervention group and 16.2±5.3 days in the control group. The intervention group healed about 1.4 times faster than the control group.
Conclusion: Autologous platelet gel can be used as a new therapeutic approach in healing burn wounds. With this product, burn wounds can be expected to heal more quickly.
Majid Naderi, Akbar Dorgalaleh, Shaban Alizadeh, Ahmad Kazemi, Hosein Dargahi, Shadi Tabibian, Mohammad Reza Younesi, Zahra Kashani Khatib ,
Volume 16, Issue 7 (10-2013)
Abstract
Background: Factor XIII deficiency is an extremely rare, autosomal recessive coagulation disorder with estimated prevalence of 1/2000000 worldwide. This disorder represents with different clinical manifestations including, umbilical cord bleeding, recurrent abortion and CNS bleeding. CNS bleeding is a common but life threating complication of disease. The aim of this study was to evaluate the effect of a common polymorphism of thrombin activatable fibrinolysis inhibitor (TAFI) occurrence of CNS bleeding in patients with severe factor XIII deficiency.
Materials and Methods: This case control study was performed on 34 patients with factor XIII deficiency and history of CNS bleeding and 36 patients with factor XIII deficiency but without CNS bleeding as control group. Initially all patients were molecularly analyzed for factor XIII deficiency, then both groups were assessed for common TAFI Thr325Ile polymorphism. Finally obtained data was analyzed by SPSS software.
Results: Molecular analysis of TAFI Thr325Ile polymorphism revealed that almost all patients with CNS bleeding (89%) had this mutation that in 67% of patients was homozygote. There is a significant relationship between Thr325Ile polymorphism in homozygote manner with incidence of CNS bleeding in factor XIII deficient (OR 18.9, 95% CI 3.8 to 95.1).
Conclusion: It seems that Thr325Ile polymorphism is a suitable prognostic factor in patients with severe factor XIII deficiency and this probably polymorphism increases risk of CNS bleeding about 20 fold.
Elahe Aslani, Nooshin Naghsh, Monire Ranjbar,
Volume 16, Issue 10 (1-2014)
Abstract
Background: Chronic myeloid leukemia (CML) is a malignant clonal disorder of hematopoietic stem cells which results in increase of myeloid cells, erythroid cells and platelets in the peripheral blood and hyperplasia in bone marrow. The research evaluates the cytotoxic effect of hydro-alcoholic extract of M.polegium before flowering aerial organs on K562 cell line as a model of chronic myeloid leukemia.
Materials and Methods: In this experimental trial, Leaves and stems of M. pulegium before flowering collected from Afoos city and extraction using maceration method. K562 cells were cultured and treated with concentrations of extract (12.5-100 &mug/ml) and different times (24,48,72 hour). Cytotoxicity of M. pulegium before flowering extract against K562 leukemia cells was estimated by the MTT test method. The absorbance was measured using an ELISA plate reader at 540 nm. Survey on data accomplished with the use of SPSS15 software and one-way ANOVA test analysis and Tukey test and p<0.001 was considered significant.
Results: Hydro-alcoholic extract of Mentha pulegium before flowering showed the highest cytotoxic effect (IC50=50 &mug/ml) and 72 hour after treatment on K562 cell line .in other words, hydro-alcoholic extract of Mentha pulegium before flowering extracts a dose and time dependent cytotoxic effect on K562 cell line.
Conclusion: Considering the cytotoxic effect of hydro-alcoholic extract of M.polegium before flowering aerial organs on K562 cells, the plant can be considered as a potential candidate for further studies on CML treatment.
Zeinab Aminilari, Farhad Daryanoosh, Maryam Koshkie Jahromi, Mohammad Mohammadi,
Volume 17, Issue 4 (7-2014)
Abstract
Background: Derived hormones from Adipokines include leptin, adiponectin, resistin, apelin, omentin.The aim of present study was to investigate the effect of 12 weeks aerobic exercise on plasma levels of Apelin, Omentin and glucose in obese older women with diabetes type 2.
Materials and Methods: In this study, 30 obese older women were chosen. They were also divided in to two groups: control (n=15 Age: 53.3+5.88 BMI: 28.7+2.2) and experimental (n=15 Age: 52.6+ 7.12: BMI: 30.03+3.6) according to their fat percentage and BMI. The training program included aerobic exercises which were conducted increasingly, 3 times a week for 12 weeks. Blood samples were taken before starting the exercise training and also 24 hours after the last training session. U Mann-Whitney Test was used to evaluate the data and in order to compare each group's pretest and posttest, Wilcoxon test was used at the significance level of p&le 0.05.
Results: There were significant changes in apelin and glucose plasma levels in the training group (p=0.001 and p=0.006 respectively). Furthermore, no significant difference was observed between each group's omentin levels.
Conclusion: It was finally concluded that exercise training can facilitate the process of glucose entrance into a cell because of the continuous muscle contractions and also decrease the plasma glucose. Also each adipokine response might be depend on the intensity and duration of exercise training, separately.
Behnaz Tavasoli, Saber Ebrahimi, Rima Manafi, Fatemeh Kiani, Majid Safa, Ahmad Kazemi,
Volume 17, Issue 7 (10-2014)
Abstract
Background: Acute lymphoblastic leukemia (ALL) is the most common type of cancer in children. Currently, chemotherapy is the most effective method of leukemia cancer treatmentwhich has many side effects. New strategies in cancer therapy utilizecompounds that specifically target aberrant signaling pathways in order to reduce toxic sideeffects Indole-3-carbinl (I3C) found in vegetables has multiple anti-cancer properties because of its ability to modulate multiple cellular signaling pathways. In this study the molecular mechanism of the action of indole-3-carbinol on pre- B ALL cells was investigated.
Materials and Methods: In current study, NALM-6 cells were treated with different concentrations of I3C at specific times. Analysis of cellular DNA content was performed by flow cytometry for evaluation of cell cycle status. The protein expression of p21, p53 as well as c-Myc proteins was determined by Western blot in I3C-treated cells.
Results: Cell cycle histogram analysis showed that I3C significantly increased the percentage of G1 cells compared with non-treated cells (control)(p<0.05). The western blot analysis also indicated I3C significantly up regulated p21, p53 expression and down regulated c-Myc expression (p<0.05).
Conclusion: The G1 arrest induced by I3C is associated with down-regulation of c-Myc and up-regulation of p53 and its downstream target p21.
Farhad Daryanoosh, Maryam Mehboodi, Maryam Mortazavi, Elham Motesharee,
Volume 17, Issue 9 (12-2014)
Abstract
Background: Obestatin and leptin are two important hormones in energy homeostasis that have been discovered in recent years. The purpose of this study was to investigate the effect of eight weeks of intense aerobic exercise on plasma levels of obestatin, leptin, insulin and growth hormones in male obese Sprague Dawley Rats.
Materials and Methods: In this exprimontal study 50 male rats, with the weight range of 20±320 were taken from the Animal Laboratory of Medical Sciences in Shiraz University, and were divided into two training groups: experimental and control. The exercise program consisted of 8 weeks of intense aerobic exercise, 5 sessions per week. The exercise started at a speed of 5 meters per minute and with a slope of zero degree for 15 minutes and the intensity progressively reached up to the speed of 25 m/m and the slope of 15 degrees for 75 minutes in the last week and ended. Blood samples were taken 24 hours after the last training session and were sent to the laboratory to measure the levels of obestatin, leptin, insulin and growth hormones. SPSS software and one-way analysis of variance (ANOVA) and Pearson correlation coefficient were used to assess the results.
Results: There was a significant reduction in plasma levels of obestatin (p=0.02), leptin (p=0.02), and insulin (p=0.03), while the results showed that no meaningful difference was observed in the amount of growth hormone between the control group and intense training group (p=0.07).
Conclusion: The results of the present study have shown that after sports activities leading to weight loss, the reduction of anti-hunger hormones (obestatin and leptin) is expected.
Behnaz Tavasoli, Rima Manafi, Fatemeh Kiani, Majid Safa, Ahmad Kazemi,
Volume 17, Issue 11 (2-2015)
Abstract
Background: Doxorubicin is a chemotherapeutic agent still in widespread use in hematologic malignancies. A side effect of anthracyclines such as doxorubicin is the activation of nuclear factor-&kappaB (NF-&kappaB), a potent inducer of antiapoptotic genes, which may blunt the therapeutic efficacy of the drugs. In this study, the effect of indole -3-carbinol (I3C) on the activation NF-&kappaB and the anti-apoptotic genes whose expression is regulated by NF-&kappaB was assessed in NALM-6 cells.
Materials and Methods: NALM-6 cells were preincubated with various concentrations of I3C and then treated with doxorubicin. Cellular DNA content assay and Annexin V-FITC staining were performed by flowcytometry for evaluation of apoptosis. For assessing the effect of I3C on the expression of XIAP, survivin, and nuclear p65 proteins, NALM-6 cells were pretreated with I3C and then incubated with doxorubicin. Whole-cell and nuclear extracts were prepared for Western blot analysis. A paired t-test was conducted to evaluate the results.
Results: DNA histogram analysis of NALM-6 cells indicates a combination of I3C with doxorubicin significantly escalated the percentages of sub-G1 population cells compared with doxorubicin - only treated group (p<0.05). Annexin V-FITC staining also showed that cotreatment of NALM-6 cells with I3C and doxorubicin significantly increased the proportion of Annexin-V positive cells in comparison with the doxorubicin treated cells (p<0.05). The western blot analysis indicated I3C significantly inhibits both doxorubicin -induced nuclear translocation of p65 and the expression of doxorubicin-induced NF-&kappaB target.
Conclusion: Our results indicated that using natural non-toxic inhibitors of NF-&kappaB such as I3C in combination with anthracyclines might be a rational combination therapy for BCP-ALL cells in which NF-&kappaB is constitutively active.
Naser Mirazi, Maryam Gholami ,
Volume 19, Issue 8 (11-2016)
Abstract
Abstract
Background: Carbon tetrachloride is one of the chemical toxins, disturbing bone marrow texture and changing the serum blood proteins. In this study, the protective effect of Avicennia marina leaf extract on bone marrow texture of rats induced by carbontetrachloride is investigated.
Materials and Methods: 42 male rats were divided randomly in to 6 groups: group induced by CCl4 (carbon tetrachloride 1:1 with olive oil, 2 ml/kg single dose, i,p), sham(taking olive oil, 2ml/kg i,p single dose) and control (taking normal saline, 2ml/kg, i,p single dose ). Treated groups (1,2 and3): induced by carbon tetrachloride 1:1 with olive oil, 2ml/kg single dose and then after two hours treated by 200mg/Kg, 400mg/Kg and 800mg/kg AME /day for 96 hrs, i,p) After the examination the blood samples were collected from heart directly and WBC and blood proteins such as Albumin, total protein separation of serum and Sections sternum bone were analyzed. Data were analyzed by ANOVA and statistical significance differences were accepted at(p<0.05).
Results: The necrotic bone marrow texture, WBC, serum Albumin and total protein of the treatment groups showed a significant increase rather than group induced by CCl4(p<0.001).
Conclusion: The Avicennia marina leaf has active antioxidant and flavonoids compounds which probably have protective effects on bone marrow texture from toxic agents such as CCl4.
Parvin Sheydaei, Abolfazl Bayrami, Yashar Azizian, Shadi Parvinroo,
Volume 19, Issue 10 (1-2017)
Abstract
Abstract
Background: Nanoparticles are used in various applications due to unique mechanical and physicochemical properties such as their increased surface area to volume ratio and quantum effects. This study was designed to investigate the cytotoxic effects of zinc oxide nanopaticles on hematological and biochemical parameters BALB/c mice.
Materials and Methods: In this experimental study, 28 adult male mice BALB/c, were divided into four groups (one control group and three experimental groups). The mice in the experimental groups orally received Zinc Oxide nanoparticles with doses of 50, 100 and 300 mg/kg for 14 days. The control group received distillated water only. On 15th day, some hematological and biochemical parameters were studied on the blood samples collected.
Results: Results showed that Zinc Oxide nanoparticles cause changes in blood cells. In high concentration, nanoparticles increased some of factors such as white blood cells, hemoglubin, MCV and neutrophil and besides decreased amount of RBCs, pLTs, hematocrit, lymphocytes, glucose and kratenin significantly (p<0.05).
Conclusion: The findings showed that zinc oxide nanoparticles cause harmful effects due to the considerable variations in hematological and serum parameters in mice in a dose-dependent way.
Seyed Amir Dadkhahi, Ali Arash Anoushirvani, Reza Aghabozorgi,
Volume 20, Issue 2 (5-2017)
Abstract
Background: Most of the current regimens in the treatment of multiple myeloma include thalidomide. Thalidomide is a modulator of the immune system and according to several studies, its main complication is thromboembolism. The aim of this study is to compare the thromboemboli prophylactic effect of aspirin and low dose warfarin in standard risk multiple myeloma patients that treated with regimens containing thalidomide.
Materials and Methods: In this double- blind clinical trial study, sixty-six patients with multiple myeloma under treatment with thalidomide-containing regimens with standard risk for thromboembolism who were admitted to Khansari hospital, entered the study according to inclusion and exclusion criteria. The incidence of thromboembolism in these patients was evaluated.
Results: Five patients in the warfarin group and 2 patients in the aspirin group had thromboemboli. Chi square analyses showed no significant difference between groups (p=0.635).
Conclusion: The results showed that both drugs are effective in preventing thromboembolism and can be used as a prophylactic treatment.
Hengamesadat Razavi, Shaban Alizadeh, Amir Atashi, Parvin Rahmani,
Volume 20, Issue 5 (8-2017)
Abstract
Abstract
Background: Fetal hemoglobin (α2γ2) is the main oxygen transport protein in the human fetus. Fetal hemoglobin is nearly completely replaced by hemoglobin A, except in a few thalassemia cases and sickle cell anemia. Several studies have indicated that expression of γ-globin might be regulated post-transcriptionally. Small non-coding RNA called microRNAs which target mRNA can lead to translated repression or mRNA decay. The aim of this study is to investigate the effect of miR-940 up-regultion on γ-chain gene expression and erythroid markers in k562 cell line.
Materials and Methods: In this experimental study, k562 cells were cultured in RPMI1640. Then pre miR-940 was transfected by electroporation method in k562 cell line. In 3, 7 and 14 days, RNA was extracted and cDNA synthesized in selected days. Up-regulation of miR-940 was confirmed by miRNA Quantitative real time PCR and then the expression γ of chains and GATA-1 was investigated by QRT-PCR. Finally, erythroid markers were checked by flow cytometry.
Results: In 3, 7 and 14 days after transfection, the GATA-1 and γ-chain expression were increased in comparison with untransfected cells. Also, the expression of erythroid markers was increased.
Conclusion: The data show that up-regulation of miR-940 has a role in the increase of γ-chain gene expression in k-562 cell line. We suggest that miR-940 may be a significant potent therapeutic target for increasing Hb F level. Patients with sickle cell anemia and β-thalassemia are suitable candidate for treatment in this way.
Mozhgan Hashemieh,
Volume 22, Issue 5 (11-2019)
Abstract
This article has no abstract.
Masoomeh Rahimzadeh, Siroos Naeimi, Mohammad Mahdi Moghanibashi, Khalil Khashei Varnamkhasti,
Volume 23, Issue 4 (9-2020)
Abstract
Background and Aim: In acute myeloid leukemia, a large number of immature cells develop, which can related to some single nucleotide polymorphisms presence in positions of genes that encodes enzymes involved in cell activation and evolution signaling pathways. In this study, the association of rs104893674 (A / C) polymorphism with the risk of Acute Myeloid Leukemia (AML) in samples obtained from Fars and Isfahan Province hospitals was investigated.
Methods & Materials: In the present case-control study conducted at Islamic Azad University of Kazerun in 2019, 94 AML patients and 99 age and sex-matched healthy individuals were enrolled. The rs104893674 (A / C) polymorphism was determined by Tetra Primer ARMS PCR method. Data were analyzed by SPSS (version23) software using Chi-square statistical test.
Ethical Considerations: This study with research ethics code IR.IAU.KAU.REC.1398.051 has been approved by Research Ethics Committee of Islamic Azad University of Kazerun.
Results: The results of this study showed a significant, allele and genotype-specific Association between rs104893674 (A / C) polymorphism with risk of AML. Thus, there are more likely to develop AML in AC genotype, individuals with A allele at this polymorphic site (P=0.000).
Conclusion: The association of acute myeloid leukemia with the genetic polymorphism of the ZAP-70 protein can be considered as an option for prognosis of this complication in susceptible individuals.
