Showing 173 results for Rat
Marjan Hajimoradi Javarsiani, Javad Sajedianfard, Shagayegh Haghjooy Javanmard,
Volume 24, Issue 3 (8-2021)
Abstract
Background and Aim: Cancer cannot be explained only by genetic alterations but involves epigenetic processes. Modifying histones by acetylation plays a key role in epigenetic regulation of gene expression and is controlled by the balance between Histone Deacetylases (HDAC) and Histone Acetyltransferases (HAT). The HDACs expression and activity could be involved in several tumorigenesis mechanisms, so their inhibition induces cancer cell cycle arrest and migration.
Methods & Materials: Quisinostat is a novel promising second-generation HDAC inhibitor class of hydroxamic acid with high cellular potency towards classes I and II HDACs. Therefore, its low IC50 (<0.5nM) and bioavailability have been chosen to carry out our studies. Cancer cells were treated with Quiznos at nM200, and cell migration was measured by fluorescent microscopy.
Ethical Considerations: This study was the result of a preliminary study of Shiraz University (Code: 96GCU3M1293).
Results: The data showed that treatment of cancer cells with Quiznos significantly (P<0.05) reduced cell migration. DMSO did not affect reducing cell migration.
Conclusion: In this project try to explore the possible therapeutic application of this HDAC inhibitor against colon cancer. This study showed Quisinostat exerts broad-spectrum antiproliferative activity and migration.
Dr Hamidreza Soodagar, Dr Ali Farhadimahalli, Ms Hamideh Rezaei, Sir Mousa Reza Es'haqgh-Pour Rezaee,
Volume 24, Issue 6 (1-2022)
Abstract
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Maryam Jafarpour, Mojtaba Bayani, Leila Hesami-Moghadam, Ali Pooladi,
Volume 25, Issue 1 (3-2022)
Abstract
Background and Aim The use of allograft alone or in combination with other bone materials has significantly improved bone-augmentation procedures. Therefore, this study aimed to evaluate the effect of topical gentamicin solution (80 mg) with allograft on guided bone regeneration in defects in rat skull.
Methods & Materials Twelve Wistar mice were randomly divided into two groups, with 6 in each group. Three defects were created in their skulls, one filled with demineralized freeze-dried bone (DFDBA) allograft alone, the other with allograft with gentamicin 80 mg solution, and the third defect were left empty as a control group. After 4 and 10 weeks, the mice were killed for histological specimens. Histological and histomorphometric evaluations were performed to qualitatively and quantitatively assess bone formation, a new bone type made, new bone vitality, inflammatory response, connective tissue type, and angiogenesis.
Ethical Considerations This study was approved by the ethics committee of Arak University of Medical Sciences (Code: IR.REC.ARAKMU.1397.30).
Results At the end of 4 and 10 weeks after surgery, the average percentage of ossification in the area of defects was higher in the group that received allografts with gentamicin, and during this period, a significant difference was seen between these groups and the control group.
Conclusion According to the present study, the use of gentamicin in combination with DFDBA has a significant effect on bone regeneration in defects in the rat skull.
Fatemeh Mohammadi, Maryam Nikrou, Kimia Sadeghi, Farid Askarifard, Mohammad Sayyadi,
Volume 25, Issue 2 (5-2022)
Abstract
Background and Aim Coronavirus disease 2019 (COVID-19) is a respiratory disorder that can affect many body systems, including the hemostatic system. In this study, we aim to investigate the role of hemostatic system and the blood coagulation in COVID-19.
Methods & Materials In this review study, the articles were searched using the keywords COVID-19, Respiratory infection, and Coagulopathy in Google Scholar, PubMed, Google Springer and Science Direct databases.
Ethical Considerations Ethical principles in writing this article were observed in accordance with the guidelines of the National Ethics Committee and the Committee on Publication Ethics (COPE).
Results Many changes in the coagulation profile of infected patients were reported, including changes in the platelet count, fibrinogen/fibrin degradation product, D-Dimer, fibrinogen, prothrombin time, and activated partial thromboplastin time. With the increase in the number of patients with COVID-19, several studies found out the occurrence of thrombosis and coagulopathy in patients.
Conclusion Due to the increase in the occurrence of coagulation disorders in patients with COVID-19, the administration of anticoagulants is needed for their treatment; it can play an effective role in improving the prognosis of patients.
Ms Nafise Mohamadi Farsani, Doctor Ebrahim Nasiri Formi, Ms Zohre Khodadadi Jahromi,
Volume 25, Issue 4 (9-2022)
Abstract
Introduction: The period of the epidemic of covid-19 is a critical situation where the operating room personnel are in the front line and are in more contact with the sick patient and his respiratory tract. The purpose of this study is to determine the preventive behaviors of operating room personnel of Isfahan teaching hospitals during the epidemic of Covid-19.
Methods: In this cross-sectional descriptive study, 100 operating room personnel of Isfahan teaching hospitals were investigated in 2019-1400. The required information was collected with the help of demographic information questionnaires and a standard questionnaire of specific questions related to preventive behaviors. Data collection was done from March 1999 to June 1400, then it was analyzed by SPSS statistical software. The Research Ethics Committee of Sari University of Medical Sciences has registered this study with the code IR.MAZUMS.REC.1400.115.
Results: The average rate of compliance with preventive behaviors among operating room personnel was 64.53%, which was above the average level. The highest compliance rate was preventive behaviors after the patient with corona virus left the operating room (84.50%). In this research, 100 health care personnel of operating rooms participated in the study. The gender of the majority of them (74%) was female and 63% of them were under 30 years of age. The participants stated that after the patient with corona virus leaves the operating room, they often or always dispose of the patient's consumables correctly (85%), complete disinfection. It was done with detergents (87%), using UV rays (86%), storing and transporting samples in special double-layered containers and fully complying with hygiene principles (83%).
Conclusions: The results showed that at the time of admission of the patient without Covid 19, contraceptive protocols were observed to a lesser extent than when the patient with Covid was admitted. Preventive behaviors and staff knowledge and awareness about the proper use of personal protective equipment can play an effective role in reducing their incidence.
Dr Roghayeh Kiyani, Ms Haleh Behroti,
Volume 26, Issue 2 (7-2023)
Abstract
Introduction: Adverse effects of Coronavirus on somatic and mental health is undeniable .nurses staff are exposed to risk of somatic and psychological effects because of continuous working time. The aim of this study was to investigate the effectiveness of training coping strategies with stress on distress tolerance and psychological empowerment of corona nurses in Khoy hospitals.
Methods: This research was applied in terms of purpose and quasi-experimental in terms of research method with pre-test – post-test design with control group And from the statistical population that included all nurses in the corona ward of Khoy hospitals in 2021 , 30 people were selected as the available sample. The subjects were randomly assigned into two groups (15 individuals in experimental group and 15 individuals in control group).In order to collect data, the Distress Tolerance Questionnaire and the Psychological Empowerment Questionnaire were used. The experimental group subjects participated in eight 90-minute sessions of training coping strategies with stress. Multivariate analysis of covariance was used to test the hypotheses. This study was approved by the Ethics Committee of Islamic Azad University of Tabriz (Code: REC.1400.135).
Results: The results showed that training coping strategies has increased distress tolerance and psychological empowerment in the post-test phase (P < 0.01).
Conclusions: According to the findings of the present study, it can be concluded that teaching coping strategies can be used as an effective method to improve stress tolerance and psychological empowerment of coronary nurses.
Dr Mehdi Asghari, Dr Gholamabbas Fallah Ghalhari, Dr Marzieh Abbasinia, Dr Farbod Ebadifard Azar, Dr Fahimeh Shakeri, Dr Mohammad Javad Ghanadzade, Mr Gholam Heidar Teimori, Dr Sanaz Karpasand, Dr Hossein Safari,
Volume 26, Issue 3 (9-2023)
Abstract
Introduction: One of the biggest environmental challenges of the 21st century is the phenomenon of climate change and global warming, which can cause numerous health problems. One significant health issue associated with climate change is the increase in the prevalence of heat stress, particularly in occupational encounters in hot outdoor environments. So this study aimed to predict and model temperature and humidity indices in the coming decades in a dry climate.
Methods: This study obtained daily atmospheric parameters, including minimum temperature, maximum temperature, and relative humidity of Kerman station, representing a dry climate, for a period of 30 years (1965 to 2005) from the meteorological organization. Modeling of these parameters and calculation of the Temperature and Humidity Index (THI) were performed using the atmospheric general circulation model HadGEM2-ES and the CORDEX dynamic microscaler model. Three different radiative forcing scenarios, including optimistic (RCP 2.6), intermediate (RCP 4.5), and pessimistic (RCP 8.5), were considered for the coming decades (three 30-year periods from 2011 to 2099). The Ethics Committe of Iran University of Medical Sciences (IUMS) approved the present study (Code: IR.IUMS.REC.1400.020).
Results: The results of the study showed an increasing trend for minimum and maximum temperature parameters in all three time periods using the three scenarios considered. Moreover, the increase in temperature in the decades of 2099-2071 was more pronounced compared to the previous decades. The results also demonstrated an increasing trend in the THI index based on all three scenarios. The average results obtained using these scenarios showed an increase of 2.11, 2.53, and 3.3 degrees Celsius, respectively, in this index until the end of the last century compared to the base decades.
Conclusions: Overall, the increase in temperature and the investigated THI index in the studied station, based on all three scenarios, will lead to changes in thermal comfort. Higher levels of thermal discomfort will not only occur in hot months and seasons but also in other seasons. Therefore, proper planning should be implemented to address the adverse effects of climate change on people's health.
Saeed Pazhoohan, Fatemeh Abbasi Feijani, Abdollatif Moini , ,
Volume 26, Issue 6 (2-2024)
Abstract
Chronic obstructive pulmonary disease (COPD) is a progressive and debilitating condition. People with COPD often experience exacerbations that may require hospitalization. Pattern analysis of respiratory variability can provide valuable insights into the complexity of the respiratory control system. Therefore, this study investigated respiratory pattern variability during stable and exacerbation phases in patients with COPD.
We analyzed respiratory signal data from 14 patients with COPD during exacerbations and stable phases and compared them with 12 age- and sex-matched control subjects. Respiratory pattern variability analysis of 30-minute inter-breath intervals (IBI) time series was performed using sample entropy and Detrended Fluctuation Analysis.
Sample entropy analysis of the IBI revealed that respiratory variability was more regular during both stable and exacerbation phases in patients with COPD. Also, the short-term (α1) and long-term fractal-like correlation (α2) significantly decreased during both exacerbation and stable phases compared to healthy controls.
The respiratory control system in patients with COPD shows less variability (lower entropy and fractal correlation). This reduction in respiratory signal variability indices in COPD patients is still lower than in healthy people, even when their disease status is stabilized.
Habib Rezaei, Soad Mahfoozpour, Somayeh Hesam, Eiravan Masoodi Asl,
Volume 27, Issue 2 (5-2024)
Abstract
Introduction: Traffic accidents are a principal challenge in the injury and death of people in Iran and many other countries, which has made the formulation of necessary strategies and planning for inter-sectoral cooperation to prevent and reduce traffic accidents undeniable. Therefore, this research was conducted to investigate the dimensions of inter-sectoral collaboration in preventing traffic accidents in Iran.
Methods: This research is a qualitative study that was conducted in 2023. In this study, sampling was done in a targeted manner, and 15 people from the statistical population were interviewed. The statistical population of the research at this stage included executive managers, traffic police, field activists, and public institutions active in the field of traffic accidents in Central Province and Tehran, who, due to the relevance of their job field, were informed or participated in intersectoral cooperation in line with the management of accidents and traffic accidents were included in the study. Data were collected and analyzed through structured interviews, and their coding was done with the help of MAXQDA 11 software.
Results: The study identified and extracted eight main dimensions of intersectoral cooperation in preventing traffic accidents. These dimensions include laws and law enforcement, research and development, information and data management, internal and external coordination, crisis management, education and awareness, roads, and vehicles.
Conclusions: Providing platforms for promoting the development and expansion of inter-sectoral cooperation in accident prevention and traffic accidents can have various benefits, including long-term social, political, economic and, well-being. Therefore, based on the findings of the research, it is suggested to design an intersectoral cooperation model for the prevention of accidents and traffic accidents so that traffic accidents can be reduced to the minimum possible with proper planning and design so that we can witness the highest level of effectiveness and efficiency in this area.
Mohammad Ali Gharaat,
Volume 27, Issue 4 (10-2024)
Abstract
Introduction: Physical activity causes cardiac hypertrophy by a non-pathological change in cardiac structure, called physiological hypertrophy. Several molecular changes are involved in this process. Insulin-like growth hormone-1 (IGF-1) is an important hormone involved in hypertrophy of cardiomyocytes. Also, the role of GATA4 as a possible gene involved in cardiac hypertrophy is controversial. Therefore, the present study was conducted to find the effect of aerobic and interval training on IGF-1, GATA4 gene, and cardiac tissue.
Methods: Eighteen male Wistar rats (243.72 ± 23.41 g) were randomly divided into control, aerobic, and interval training groups (n = 6). The aerobic group trained for 8 weeks/4 days a week/38 minutes/ 65% of maximum oxygen consumption (VO2 max). The intermittent group trained 8 weeks/4 days a week/5 set/4 minutes/85-90% of VO2 max running with 2 minutes rest between the sets. The weight of the heart and left ventricle, IGF-1 concentration, and GATA4 gene expression were measured 48 hours after the training. The evaluation was conducted with a one-way analysis of variance and Tukey's post hoc test at a significance level of 0.05.
Results: The weight of the heart and left ventricle increased significantly in trained groups. IGF-1 and GATA4 gene expression increased in both the training groups than the control group.
Conclusions: Aerobic and Interval training increases IGF-1, GATA4 gene, left ventricle, and heart weight. Considering the shorter time interval training takes, it seems that interval training is more beneficial than aerobic training.
Atefeh Khaki, Maryam Baazm, Mohamad Bayat,
Volume 27, Issue 5 (12-2024)
Abstract
Introduction: Glyphosate is the most popular broad-spectrum herbicide globally due to the growing demand for glyphosate-resistant crops. Glyphosate exhibits harmful properties, including cytotoxicity, neurotoxicity, and reproductive toxicity. This study aimed to investigate the detrimental effects of glyphosate on ovarian histopathology in mice and the in vitro maturation (IVM) of oocytes following superovulation.
Methods: In this study, thirty-two female NMRI mice were randomly divided into the following groups: control, glyphosate, superovulation, and superovulation-glyphosate. Animals received glyphosate (0.5%) continuously through drinking water for three weeks. HMG and HCG were used to induce superovulation. Oocytes were collected from the ampulla, and the quantity and quality of oocytes were analyzed. Then, in vitro maturation (IVM) of oocytes was performed. At the end of the study, ovarian histopathology was analyzed.
Results: Compared to the control group, the glyphosate-treated group exhibited a significant decrease in secondary and Graafian follicles while demonstrating a concomitant increase in atretic follicles (P < 0.05). Additionally, the superovulation-glyphosate group showed fewer germinal vesicle breakdown (GVBD) and MII oocytes than the superovulation group. In the superovulation-glyphosate group, there was a notable reduction in GVBD and MII oocytes following in vitro maturation (IVM).
Conclusions: Glyphosate has the potential to damage ovarian tissue and adversely affect IVM and oogenesis.
Rouhollah Hemmati Bushehri, Mahmoud Reza Jaafari, Ghasem Mosayebi, Ali Ghazavi, Ali Ganji,
Volume 27, Issue 6 (1-2025)
Abstract
Introduction: Nowadays, plant-derived compound is widely used in medical treatment as complementary therapies. Rosemary is one of these plants that have anti-angiogenic and anti-tumor effects. However, low solubility and low bioavailability lead to limited use of this compound. To overcome this limitation, the use of liposomes is beneficial. So, this study aimed to prepare liposomes containing rosemary alcoholic extract to obtain the best formulation with best properties to use in the antitumor study.
Methods: Liposomes were prepared by Thin-Film-Hydration method in different ratios of rosemary extract, hydrogenated-soybean-phosphatidylcholine (HSPC), Cholesterol, and mPEG2000 (F1-F6). The characterization of nanoliposomes includes size, PDI (Poly dispersity index), zeta potential, encapsulation efficiency, and release rate was measured.
Results: The results showed that the average particle size and zeta potential were 113.4 nm and -10.8 mV, respectively. Encapsulation efficiency showed a range of 60- 90%. The average release rate of the extract was about 60%. Also, the results showed that the F1 formulation was transparent, had appropriate properties, and did not precipitate. F2 formulation was not transparent. F3-F4 formulations were precipitated, and F5-F6 formulations did not achieve the appropriate size.
Conclusions: This study proved that F1 formulation in a ratio of 55:30:5 HSPC/chol/mPEG2000 is the best formulation for the preparation of nanoliposomes containing rosemary alcoholic extract to achieve the best properties for use in cancer studies.
Fateme Tahsinin, Ali Heidarianpour, Elnaz Shokri,
Volume 28, Issue 1 (3-2025)
Abstract
Introduction: Being overweight is associated with increased cortisol secretion, along with heightened anxiety and depression. This study aimed to compare the effect of six weeks of concurrent moderate-intensity continuous training (MICT) and high-intensity interval training (HIIT) with HIIT training alone on body composition, plasma cortisol levels, and anxiety and depression levels in overweight male students.
Methods: In this semi-experimental research, 27 overweight students were selected and randomly divided into three groups 9: MICT+HIIT concurrent training, HIIT training, and a control group. The MICT )15 minutes of exercise per day at 60-65% of maximum heart rate with an additional 5 minutes every two weeks). The HIIT (3 minutes of running at 70-75% of maximum heart rate, followed by 3 minutes of active rest at 50% of maximum heart rate, with three repetitions in the first two weeks, four repetitions in the second two weeks, and five repetitions in the final two weeks).
Results: Both types of training protocols resulted in reductions in weight, body mass index, cholesterol, low-density lipoprotein, cortisol levels, anxiety, depression, and an increase in high-density lipoprotein (P < 0.05). There was no significant difference between the two types of exercise in terms of effectiveness on these variables; however, only peak oxygen consumption and triglycerides were associated with better changes in the MICT+HIIT group.
Conclusions: For overweight male adolescents aiming for weight loss and improved mental health, HIIT alone is sufficient, and adding MICT does not provide additional clinical benefits.
