Ali Ganji, Iman Farahani, , , Mohammadhasan Sakhaie,
Volume 28, Issue 6 (1-2026)
Abstract
Introduction: Experimental Autoimmune Encephalomyelitis (EAE) is an immune-mediated inflammatory model of multiple sclerosis that induces axonal loss, demyelination and motor disabilities. Electromagnetic fields (EMFs) possess multiple biological capabilities that affect the nervous system. The aim of the present study is to investigate the beneficial effect of low-intensity electromagnetic field on clinical symptoms, histological structure and gene expression in the cerebral cortex.
Methods: Forty-eight female C57BL/6 mice were used for this study. Following model induction, with MOG immunization the animals exposed to the EMFs (1 mT,50 Hz) over a period of two weeks. At the end of experiments, the body weight, clinical score (paralysis score) and lymphocyte infiltration of the cortex were evaluated in experimental groups. Moreover, the mRNA expression levels Bcl2, BDNF and Nrf2 are also were studied by using quantitative polymerase chain reaction (qPCR).
Results: The EMFs, significantly reduced the clinical symptom scores, decreased lymphocytic infiltration. The applied EMF also upregulated the expression of Bcl2, BDNF and Nrf2 in the cerebral cortex in compared to EAE model.
Conclusions: Overall, the present study demonstrated that electromagnetic fields, as a complementary therapy, exert significant neuroprotective properties in EAE by inhibiting oxidative stress and reducing neural tissue inflammation.
