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Background: One of the most common problems in Menopausal women is reduce of sexual satisfaction, which can affect their quality of life. The aim of the present study was the effect of Afrodit on sexual satisfaction in postmenopausal women.

Materials and Methods: In the triple blind clinical trial with the placebo group participated 80 healthy females 50-60 years that were referred to health centers affiliated to Tehran University of Medical Sciences. Participants were randomly assigned to two groups, Afrodit groups and placebo capsules for a month. Used tool in this study was Sabatsbrg. Part of which was to assess sexual satisfaction.

Results: After a month intervention, there is significant difference in the mean score for sexual satisfaction between two groups (p=0.01), Sexual satisfaction compared with pre-menopausal in two groups were compared. T-test results showed no significant difference. (p=0.16).

Conclusion: The results show Afrodit improves the sexual satisfaction in menopausal women. So it is essential that health providers are familiar with this herb supplement.

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Background: According to the important of SALL4 gene during the development of embryonic neurvous system, our aim in this study was to analyze and quantify mRNA expression of SALL4 in Prosencephalon during different stages of chicken embryogenesis.

Materials and Methods: In this experimental study, incubated Ross fertilized eggs were applied in 37°C-37.5°C in 60-65% humidified atmosphere after beginning of embryogenesis. Prosencephalon part of the brain tissue was collected from the eggs, daily. Total RNA extraction and cDNA synthesis was performed from resected tissues. The synthesized cDNA was used as template for quantitatively analysis of SALL4 mRNA expression by real-time PCR.

Results: The Results indicate that the level of SALL4 gene expression is significantly variable during embryogenesis. However it doesn’t show variation during the early days. The maximum copy number of SALL4 mRNA was quantified on 15 th day of chicken development.

Conclusion: SALL4 mRNA expression is high when the Prosencephalon is under development, using of HAMBURGER–HAMILTON chart, there is relation between increasing SALL4 expression and developing limbs and anterior brain.

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Background: Acne vulgaris, is a chronic inflammatory disease of the pilosebaceous units. Genetic, environmental factors and nutrition influence on this disease. Recent studies indicate an essential role of nutrition in the development of acne, particularly with the effect on oxidative stress and inflammatory processes. Many studies have reported lower incidence of acne in individuals who receive diets containing large amounts of omega-3. The aim of this study was to determine the probable therapeutic effects of omega-3 for the treatment of acne.

Materials and Methods: In this study, 60 patients with moderate to severe facial acne were randomly divided into two treatment groups: 1) 100 mg doxycycline daily and one placebo capsule Omega -3 2) doxycycline 100 mg daily and one capsule 1000 mg Omega -3. Acne severity was determined based on GAGS system at the beginning of treatment and at the end of the sixth and twelfth weeks of treatment, with photographs obtained from each patient.

Results: There was a significant reduction in acne severity score (GAGS) at the end of sixth and twelfth weeks in the Omega-3 group in comparison to control group that received placebo.

Conclusion: Omega-3 fatty acids can improve moderate to severe inflammatory acne, probably due to their anti-inflammatory properties.

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Background: Delayed muscular soreness after resistance exercises or eccentric trainings is probably because of muscle damage and injury. Nutrition by playing a crucial role in both protein synthesize and catabolism can influence the extent of muscle injury. The objective of this study was to assess the effect of single portion of Glutamine supplement consumption on injury indices of muscle after a session eccentric resistance exercise.

 Materials and Methods: This study used a randomized, double blind design that consisted of 80 volvnteer non-athletic males (aged 22.2±2.2 years, height 175±5 cm, weight 71.64±9 kg, body mass index 23.2±2.2 kg/m2, and body fat 17.5±2.4%). A total of 40 participants were divided randomly into 2 groups, supplement group (receiving 0.1 g/kg Body weight/day Glutamine) and placebo group (receiving 0.1 g/kg Body weight/day Maltodextrin). Serum keratine kinase (CK) was determined by photometric method, muscle pain and knee joint range of motion were measured using, respectively, a standard scale of PAS and goniometer before, 24 and 48 hours after a resistance test involving knee flexion.

Results: Glutamine supplement consumption caused no significant differences in CK levels reduction in none of the measured times, but it reduced the muscle pain at the times of 24 and 48 hours in comparison with the placebo group. In addition, the knee joint range of motion was significantly improved at 24 hours after the test.

Conclusion: It seems that this dose of Glutamine supplementation can reduce the apparent signs apart from muscle injury indices reduction.

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Background: Dendrimers are macromolecules with regular three-dimensional structure that have many branches. The aim of this study was to investigate the fficiency of Nano Polyamidoamine–G5 (NPAMAM-G5) dendrimers in removal of Escherichia coli, Klebsiella oxytoca, Pseudomonas aeruginosa, Proteus mirabilis and Staphylococcus aureus from aqueous solution.

Materials and Methods: In this experimental study, initially, dilution of 10³ CFU/ml was prepared from each strain of bacteria. Then, different concentrations of dendrimers (0.025, 0.25, 2.5 and 25 µg/ml) in the ambient temperature (23-25°C) was added to water. In order to determine the efficiency of dendrimers in removal of bacteria, samples were taken at different times (0, 10, 20, 30, 50 and 60 min) and were cultured on nutrient agar medium. Samples were incubated for 24 hours at 37 ° C and then the number of colonies was counted.

Results: There is a direct relation between antibacterial properties of dendrimers in aqueous solution and increasing the dendrimer concentration and contact time. At a concentration of 25 μg/ml and 60 minutes, all kinds of bacteria except Staphylococcus aureus, and at 30 minutes, E. coli and Klebsiella bacteria, were removed by 100% The concentration of 2.5 μg/ml at 60 minutes of  bacteria, Escherichia coli, Klebsiella and Proteus mirabilis, 100% Were removed. All concentrations of dendrimer at different times were reduced bacteria.

Conclusion: Results of this study indicated that NPAMAM–G5 dendrimer is able to remove Escherichia coli, Klebsiella oxytoca, Pseudomonas aeruginosa, Proteus mirabilis and Staphylococcus aureus from aqueous solution.

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Background: A small percent of patients with colorectal cancer (CRC) respond to 5-Fluorouracil (5-FU), as a first line of chemotherapy. In this study in, in order to design a new chemotherapy protocol, the effect of 5-FU and acriflavine (ACF) cotreatment on mortality rate of CRC cell lines was investigated.

Materials and Methods: Cytotoxicity of 5-FU and ACF against CRC cell lines (LS174T, SW480 and HCT116) was detected using MTT assay. Cells were treated with different concentrations of 5-FU (0.5-64 µM) or ACF (0.07-5 µM) for 72 hours and then cell viability and drugs IC50 was calculated. To assess the effect of ACF on anticancer activity of 5-FU, cells were cotreated with different concentrations of 5-FU and IC30 concentration of ACF.

Results: ACF and 5-FU suppress the viability of CRC cell lines in dose-dependent manner. 5-FU and ACF have most cytotoxic effect on LS174T and the lowest cytotoxic effect on SW480 cells. Cotreatment of ACF with 5-FU could not significantly change the sensitivity of cells against 5-FU (p>0.05).

Conclusion: In this study, the fatal and cytotoxic effect of ACF on three CRC cell lines was shown. However, cotreatment of ACF with 5-FU could not improve the anticancer activity of 5-FU.

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Abstract

Background: The incidence of gastric cancer is different in two sexes with ratio 2 to 1 that it is more common in men. The most important biologically reason is sexual hormones between two sexes that lead to sexual dimorphism and in turn can cause a sex bias in incidence of disease between two sexes. Recently, studies have shown that microRNA is involved in sexual dimorphism in gene expression. Given the sexual dimorphism in the incidence of gastric cancer and sex hormones response elements in the regulatory regions of miR-146a and miR-148a genes, in this study, the expression of these two genes in the stomach of healthy men and women at different age groups were compared.

Materials and Methods: Using endoscopy, gastric antrum tissues of 35 healthy women and 35 healthy men were collected. After RNA extraction and synthesis of cDNA, the expression of miR-146a and miR-148a genes were compared between sexes by Real time RT-PCR and data were analyzed using independent sample t and ANOVA tests.

Results: There was no difference between men and women in genes expression of miR-146a and miR-148a. However, expression of miR-146a gene was significantly more in men under 45 years than men over 45 years (p= 0.017, df= 14,  t= 1.47). Also,  expression of miR-148a gene was significantly more in men over 45 years than men under 45 years (p=0.001, df= 12, t= 1.28). But the expression of both genes had no significant difference between women under 45 years and women over 45 years.

Conclusion: Expression of miR-146a and miR-148a genes in the stomach is increased and decreased with aging in men, respectively.

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Background and Aim: In acute myeloid leukemia, a large number of immature cells develop, which can related to some single nucleotide polymorphisms presence in positions of  genes  that encodes enzymes involved in cell activation and evolution signaling pathways. In this study, the association of rs104893674 (A / C) polymorphism with the risk of Acute Myeloid Leukemia (AML) in samples obtained from Fars and Isfahan Province hospitals was investigated. 
Methods & Materials: In the present case-control study conducted at Islamic Azad University of Kazerun in 2019, 94 AML patients and 99 age and sex-matched healthy individuals were enrolled. The rs104893674 (A / C)   polymorphism was determined by Tetra Primer ARMS PCR method. Data were analyzed by SPSS (version23) software using Chi-square statistical test.
Ethical Considerations: This study with research ethics code IR.IAU.KAU.REC.1398.051 has been approved by Research Ethics Committee of Islamic Azad University of Kazerun.
Results: The results of this study showed a significant, allele and genotype-specific Association between rs104893674 (A / C) polymorphism with risk of AML. Thus, there are more likely to develop AML in AC genotype, individuals with A allele at this polymorphic site (P=0.000). 
Conclusion: The association of acute myeloid leukemia with the genetic polymorphism of the ZAP-70 protein can be considered as an option for prognosis of this complication in susceptible individuals. 

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Background and Aim: Proliferate potential differentiate into different cell lineages and high self-renewal of Mesenchymal Stem Cells (MSCs); thus, they are ideal tools for regenerative medicine. However, a leading problem is an oxidative stress in the target tissue and the apoptosis of transplanted stem cells before tissue repair. The pretreatment of stem cells with antioxidants may make them resistant to oxidative stress. Ginger is the main medicinal plant with antioxidant properties. This study explored the antioxidant effects of ginger extract on bioavailability and oxidative stress-induced apoptosis in human adipose tissue-derived mesenchymal stem cells and rat bone marrow examined. 
Methods & Materials: In this study, human adipose tissue-derived mesenchymal stem cells and rat bone marrow were cultured in a DMEM medium with 20% FBS. The explored cells were incubated for 4 and 6 hours for pretreatment with different concentrations of ginger extract (50, 100, 200, & 400 mg/mL); then, they were treated with 200 μM H2O2 for 2 hours. Bioavailability was analyzed by ELISA reader using an MTS kit and apoptosis was analyzed by flow cytometry using an Annexin V-FITC/PI kit into the manufacturer’s protocol at both times. The obtained data were analyzed by Analysis of Variance (ANOVA) using SPSS. 
Ethical Considerations: This study was approved by the Ethics Research Committee of Shahrekord Branch, Islamic Azad University (Code: IR.IAU.SHK.REC.1397.028).
Results: The MTS results indicated a dose- and time-dependent manner increase in the bioavailability of human adipose tissue-derived mesenchymal treated stem cells. Ginger extract treatment also dose- and time-dependently decreased the rate of apoptosis in rat bone marrow mesenchymal stem cells. 
Conclusion: Ginger extract, by reducing the oxidative stress in mesenchymal stem cells, elevates their lifespan in the target tissue, and increases the efficiency of these cells in tissue regeneration.

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Background and Aim: This study aimed to investigate the effect of exercise timing on elevated postprandial glucose and after brief interval exercises in women with obesity.
Methods & Materials: Ten women with obesity (Mean±SD age = 40.41±3.97 years; weight = 86.66±7.26 kg; and BMI = 33.22±2.20 kg/m2) participated in a crossover design exercise intervention: 1) postprandial aerobic exercise, 2) pre-prandial aerobic exercise, 3) brief periodic exercise, and 4) control. Pre- and postprandial exercise included 30 min of moderate-intensity walking on the treadmill before and after each main meal (1 minute of exercise -30 seconds rest). The brief periodic exercise had three one-minute reps of activity every 30 min for 20 times during the day. Twelve-hour continuous glucose monitoring and two-hour postprandial glucose levels were calculated to examine changes in blood glucose levels.
Ethical Considerations: This study was approved by the institutional review board of the University of Isfahan (Ethics Code: IR.UI.REC.1397.119) and conducted in agreement with the ethical principles for biomedical research involving human subjects outlined in the declaration of Helsinki.
Results: The findings of this study suggested that brief periodic exercise resulted in a significant decrease in continuous glucose monitoring levels and postprandial glucose compared to the control group as well as pre-prandial exercise (P˂0.05). However, pre- and postprandial exercise did not result in significant changes in continuous glucose monitoring (P˃0.05). In addition, postprandial exercise led to a significant decrease in postprandial glucose compared to the control group (P˂0.05).
Conclusion: It seems that brief periodic exercise can have more beneficial effects on postprandial glucose levels, probably due to improved glucose metabolism in skeletal muscle.

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Background and Aim: Women are the central pillar of social development and the main axis of family health. Success, survival, and promotion of society depend on the health of women in society. Since Health Promotion lifestyle and psychological well-being are important determinants of health, this study was conducted to determine the Health Promotion lifestyle and psychological well-being in women attending the health centers of Arak City in 2019.
Methods & Materials: In this cross-sectional study, among women who attended health centers in Arak City, 221 women who met the inclusion criteria were included in the study by continuous sampling and the form of demographic information, health promoting lifestyle profile-Ii (HPLP-II), and Ryff Psychological well-being Questionnaire was completed. Data were described using descriptive statistics of frequency, frequency percentage, mean, standard deviation, and data analysis by analysis of variance, independent t-test, and regression using SPSS software v. 19.
Ethical Considerations: This study was approved by the Ethics Committee of Research Deputy of Iran University of Medical Sciences (Code: IR.IUMS.REC.1398.609).
Results: The mean health-promoting lifestyle in the study units was 135.96; the mean physical activity components were 16.31; The mean nutrition was 26.41, with the lowest and highest scores, respectively. The mean psychological well-being in the study units was 72.29. The mean components of purpose in life were 10.76, and the mean personal growth was 13.19, with the lowest and highest scores, respectively. Age, chronic diseases, employment, number of children, and alcohol consumption had a significant negative relationship with a health-promoting lifestyle, and a woman’s and her husband’s education had a significant positive relationship with a health-promoting lifestyle (P<0.05). Overall demographic variables predict as much as 13% of health-promoting lifestyles. Age, number of children, and chronic physical and psychological diseases had a significant negative relationship with psychological well-being and economic status. A woman’s and her husband’s education had a significant positive relationship with psychological well-being. Overall, demographic variables predict as much as 10% of psychological well-being. 
Conclusion: Considering that health-promoting lifestyle and psychological well-being are two critical determinants of health, studying these two indicators and the factors affecting them can help Intervention planning to promote women’s physical and mental health.

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Introduction: The present study findings aimed to investigate the effect of exercises based on the American National Academy of Sports Medicine principles (NASM) on walking kinetics in piriformis syndrome in middle-aged men.
Methods: The current research was semi-experimental and laboratory-type. The statistical sample of the present study was 30 men with piriformis syndrome. Subjects were equally and randomly placed in two intervention and control groups. The intervention group performed NASM exercises for eight weeks. Before and after the exercises, the ground reaction force variables were measured using a Bartek force plate device with a sampling rate of 1000 Hz. Kinetic data were smoothed using a fourth-order Butterworth filter with a frequency cutoff of 20 Hz. For statistical analysis, analysis of variance and paired T-test were utilized at the significance level of P < 0.05.
Results: The findings of the present study showed that the effect of time on the FxHC and FyHC components at the peak of the forces and the FyHC component at the time of reaching the peak of the ground reaction force increased in the post-test compared to the pre-test. P > 0.025;
d = 0.64 - 0.96. The effect of the time factor in the FyPO component at the peak of the ground reaction force in the post-test was less than the pre-test (P < 0.025; d = 1.64-0.96). The interaction effect of the time × group in the FxPO component at the peak of the force and the FzHC component at the time of reaching the peak of the ground reaction force had a significant difference (P < 0.048; d = 0.87-0.83).
Conclusions: The NASM exercises used in this research can have a clinical and therapeutic effect that can reduce damage to the lower limbs and improve the quality of walking in people with piriformis syndrome.

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Introduction: Nowadays, the use of artificial intelligence and machine learning has impacted all fields of study. Utilizing these methods for identifying individuals' emotions through integrating audio, text, and image data has shown higher accuracy than conventional methods, presenting various applications for psychologists and human-machine interaction. Identifying human emotions and individuals' reactions is crucial in psychology and psychotherapy. Emotional identification has traditionally been conducted individually and by analyzing facial expressions, speech patterns, or handwritten responses to stimuli and events. However, depending on the subject's conditions or the analyst's circumstances, this approach may lack the required accuracy. This paper aimed to achieve high-precision emotional recognition from audio, text, and image data using artificial intelligence and machine learning methods.
Methods: This research employs a correlation-based approach between emotions and input data, utilizing machine learning methods and regression analysis to predict a criterion variable based on multiple predictor variables (the emotional category as the criterion variable and the features, audio, image, and text variables as predictors). The statistical population of this study is the IEMOCAP dataset, and the data type of this research is a mixed quantitative-qualitative approach.
Results: The results indicated that combining audio, image, and text data for multi-modal emotional recognition significantly outperformed the recognition of emotions from each data alone, exhibiting a precision of 82.9% in the baseline dataset.
Conclusions: The results demonstrate a considerably acceptable precision in identifying human emotions through audio integration, text, and image data compared to individual data when using machine learning and artificial intelligence methods.

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Introduction: Breast cancer is one of the most common cancers and the principal cause of death in women. One of the mechanisms of cancer cells for the lack of access to the immune system is the production of compounds suppressing immune responses, such as interleukin-10. On the other hand, vascular endothelial growth factor, by binding to its receptors on the surface of endothelial cells, plays a significant role in vascular permeability and tumor vascularity. In this study, the expression of interleukin-10 and vascular endothelial growth factor in breast tumor tissue was investigated in an experimental tumor model.
Methods: First, mammary tumors were experimentally induced in Balb/C mice, and RNA was extracted from the tumor tissue. cDNA was synthesized from the extracted RNAs, and the expression level of 10-IL and VEGF genes was evaluated by RT-PCR.
Results: The results of data analysis showed that the expression of IL-10 and VEGF genes in the tumor tissue was higher than in the cells of the control group, but this increase in expression was not statistically significant.
Conclusions: In general, the expression level of Interleukin-10 and VEGF genes was increased in the experimental tumor model, but broader research and the correlation with other involved factors seem necessary.

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Introduction: One of the problems during adolescence is the existence of conflict with parents, which creates object relationships and feelings of shame and guilt in them. This research was conducted to determine the effectiveness of treatment based on the mentalization of object relations and feelings of shame and guilt in conflicting mother-child relationships.
Methods: This research method was semi-experimental and pre-test-post-test with a control group. The statistical population of this research included all mothers and adolescents with conflicting relationships who were referred to counseling centers in Shiraz in 2022. By using the available sampling method, 30 people from the mentioned community were selected as a sample and were randomly divided into two experimental (15 people) and control (15 people) groups. Asadi et al.'s parent-child conflict questionnaires (2010), Bell's object relations (1995), and Cohen et al.'s (2011) feelings of shame and guilt were used to collect information. The experimental group underwent executive function training for 13 sessions twice a week for 60 minutes, and the control group did not receive any training. The research data was analyzed using correlation.
Results: The mean ± standard deviation of the experimental group in the post-test in the variable of object relations from 1.5 ± 1.37 to 28.6 ± 4.3 and in the variable of shame and guilt from 5 ± 1.60 to 5.60 ± 3. 51.2 ± 9.1 decreased. The results showed that in the post-test stage, the mean scores of object relations and feelings of shame and guilt in the experimental group decreased significantly compared to the control group (P < 0.01).
Conclusions: Treatment based on mentalization was effective in improving object relationships and reducing feelings of shame and guilt. Therefore, this treatment can be suggested to reduce the conflict between adolescents and mothers.