Showing 49 results for Mosayebi
Mojtaba Sharafkhah, Ghasem Mosayebi,
Volume 16, Issue 2 (5-2013)
Abstract
Background: Epidemiologic studies show the high prevalence of some infections and cancers in individual blood groups. Perhaps, level of immunity factors differs in people with individual blood group. The aim of this study is to compare the level of antibodies and complement factors in ABO blood groups.
Materials and Methods: In this descriptive study, peripheral blood samples from 40 male healthy individuals with different ABO blood groups (n=10 in each group) with similar age (18-25 years) and genetic background were collected. The serum levels of total IgG, IgM, IgA, C3, and C4 were analyzed by nephelometry method. All data were analyzed by SPSS software version 11.5.
Results: The levels of IgA, IgG, and C4 protein in individuals with O blood group were significantly higher than those of other groups (p=0.02, p=0.01, and p=0.004, respectively). Also, the mean concentration of IgM in individuals with AB blood group was significantly higher than that in other groups (p=0.02). There was a significant difference between the level of C3 in O blood group and those of other blood groups (p=0.01). The mean concentrations of all parameters (except IgG) in B blood group were lower than those in other blood group.
Conclusion: Generally, natural antibodies in O blood group were higher than those in other groups. This high level of total antibodies in O blood group may reduce susceptibility to some infections.
Ahmad Reza Behruzi, Mohammad Hossein Hadi, Ahmad Reza Ghandi, Ghasem Mosayebi,
Volume 16, Issue 11 (2-2014)
Abstract
Background: Osteoporosis is characterized by low bone density and loss of structural integrity which can increase the risk of bone fracture and its side effects. In the recent studies, new evidences of the relationship between immune system such as TNF-&alpha and IL-17A and bone destruction have been accumulated. The aim of present study was to investigate serum level of TNF-&alpha and IL-17A in women with post-menopausal osteoporosis.
Materials and Methods: In this analytical study 40 women with post-menopausal osteoporosis and 10 healthy women were enrolled to the study based on inclusion and exclusion criteria. The serum level of TNF-&alpha and IL-17A were calculated by using ELISA method and compared between groups.
Results: The mean level of TNF-&alpha in case and control group was 957.7±479.01 and 418.09±176.7 respectively which in case group was significantly more than control group (p=0.001). The mean level of IL-17A in case and control group was 95.23±36.7 and 125.7±30.6 respectively which in case group was significantly less than control group (p=0.019).
Conclusion: The level of TNF-&alpha in women with post-menopausal osteoporosis was more than healthy women which can mention the roll of TNF-&alpha in pathogenesis of osteoporosis and potential therapeutic roll of anti TNF-&alpha agents but because of unclear roll of IL-17A in pathogenesis of osteoporosis we could not explain its mechanism in pathogenesis of osteoporosis. It seems more studies with regard to IL-17A roll in bone metabolism is required.
Mahdieh Mondanizadeh, Ghasem Mosayebi, Ehsan Arefian, Massoud Saidijam, Behzad Khansarinejad,
Volume 17, Issue 2 (5-2014)
Abstract
Background: Although miR-124 molecule has been known as an inducer of neurogenesis, few researches have been done on the targets of this molecule and its functional mechanisms in differentiation toward neurons and maintaining neuronal state. The microarray technique has been established as the reference method for studying the genes under the control of miRNAs. However, the high cost of this method has hampered its use in most research centers. On the other hand, the improvement of bioinformatical algorithms and computer modeling systems has led to the development of the bioinformatical softwares that can predict mRNA targets for miRNAs. Therefore, the aim of this theoretical study was to bioinformatically evaluate the effect of miR-124 on transcription factors that can be involved in neurogenesis and neuronal cell amplification, by using various specific softwares. Materials and Methods: Using different algorithms in TargetScan, DIANA and miRWalk databases, the potential transcription factors targets of miR-124 were identified. Then, a score table was prepared from the candidate genes, based on the affinity of the seed region of miR-124 and the number of targets in the 3`-UTR region of transcription factors. Finally, transcription factors with higher scores were chosen as candidates for practical analysis.
Results: The results of bioinformaical analysis showed that the LAMC1, ITGB1, PTBP1, SOX9, SP1, and EFNB1 molecules are the most potential factors that might be affected by miR-124 during neurogenesis.
Conclusion: It seems that transcription factor SP1 is under the control of the miR-124 and plays a crucial role in neurogenesis process. Therefore, this protein can be considered as a suitable new candidate for experimental evaluation.
Mana Shojapour, Ghasem Mosayebi, Fardin Faraji, Keyvan Faraji, Ali Ghazavi,
Volume 17, Issue 3 (6-2014)
Abstract
Background: Multiple sclerosis (MS) is an autoimmune disorder with unknown etiology. Genetic and environmental factors associated with MS susceptibility. Genetic studies show an important role for human leukocyte antigens (HLA) and susceptibility to autoimmune diseases such as MS. There is controversy between the association of HLA alleles with MS susceptibility in various studies. However, with consider the high incidence of MS in Iranian population and limit information about association of HLA and MS, we analyzed HLA alleles in MS patients.
Materials and Methods: In this case-control study, 60 MS patients and 40 normal individuals with the same ethic background and geographic area were analyzed for HLA-DRB and DQB alleles by single specific primer-polymerase chain reaction (SSP-PCR) method.
Results: HLA-DRB1*03 and DQB1*02 alleles frequencies in MS patients were greater than healthy controls. There was no significant difference in frequency of other HLA-DR alleles between the MS patients and normal individuals.
Conclusion: DRB1*03 and DQB1*02 alleles confer increased susceptibility to MS in this population. However, to determine the role of HLA in Iranian MS patients, more studies are needed.
Khadijeh Khosravi, Nader Zarinfar, Ehsanollah Ghaznavi Rad, Ghasem Mosayebi,
Volume 18, Issue 4 (7-2015)
Abstract
Background: Brucellosis is a highly contagious zoonotic disease between humans and animals known for high frequency of relapsing and ability to cause chronic infection. The function of immune system plays an important role in induction of chronic diseases. However, the role of immune system response is not completely studied. Therefore, this study designed to investigate the cytokine profile of the patients suffering from chronic and acute brucellosis.
Materials and Methods: This descriptive- analytical study was performed on 22 patients with acute brucellosis (mean age 38±17), 21 patients with chronic brucellosis (mean age 43±10) and 21 healthy people (mean age 26±4) with the same age and sex as patients. The serum IFN-&gamma, IL-17, IL-5 and TGF- &beta levels were measured using ELISA method.
Results: The mean IFN-&gamma serum level in acute and chronic brucellosis patients group was significantly higher than control group (p=0.045). The mean IL-17 serum level in acute brucellosis patients was significant decreased once compared with control group and in chronic patients was significantly decreased when compared with control group (p=0.024). In addition, the mean IL-5 and TGF-&beta serum levels of acute brucellosis patients group were significantly decreased as compared to chronic patients (p=0.001).
Conclusion: The results of current study indicate that cytokine profile of chronic brucellosis patients is more related to Th2 immune response. Hence, Th2 immune response inhibition would be an appropriate way to treat and prevent disease to become chronic.
Masoud Motalleby, Ashraf Mazaheri, Mohadeseh Mosayebi, Sayyed Mehdi Takhtfiroozeh,
Volume 18, Issue 5 (8-2015)
Abstract
Background: The air pollutants such as CO, SO2, NO2, O3, and particulate matters have harmful effects on public health. Determination of the actual concentration of the pollutants and description of air quantity and quality contents in comparison of standard conditions and timely informing people to regulate control programs is essential. Kashan is exposed to the winds contain-ing the suspended particulate matters due to the proximity of the desert. Moreover, the growth of population, factories and industries in the city are artifical resources of the air pollution. Hence, assessment and monitoring of air pollution standard condition in kashan is crucial.
Materials and Methods: In this cross-sectional and descriptive study, the concentration of CO, SO2, NO2, O3, and suspended particulate matters less than 10 microns (PM10) measured according to WHO standards in Panzdah-e-Khordad station of Kashan in 2011. The annual mean and maximum rates, the mean and maximum rate of summer and winter, and annual percentile 98%, determined for each pollutant and used in AirQ software. Then, the number of death and disease attributed to each pollutant was calculated.
Results: The results demonstrate that the cumulative number of deaths attributed to PM10, NO2, SO2, and O3 was 100, 22, 82, and 54, respectively.
Conclusion: In total, the suspended particulate matters have the most effects on death and disease resulted from the air pollution. Hence, managing the resources of particulate matters and SO2 pollutants has many effects on reducing the adverse health effects of air pollution in Kashan.
Somaye Pormosayebi, Mohsen Shamsi, Mahbobeh Khorsandi, Ali Kolivand, Mehdi Ranjbaran,
Volume 18, Issue 8 (11-2015)
Abstract
Background: Today, environmental pollutants are one of the most important human problems. The purpose of this study was to assess the health belief model structures for promotion of preventive behaviors in pregnant women exposed to air pollution in Arak city in 2014.
Materials and Methods: In this cross-sectional study, 208 pregnant women referred to healthcare centers in Arak city were selected by multistage sampling and were studied. Data were collected by using a valid and reliable self- report questionnaire. The questionnaire included the demographic characteristics of the mother, the aspects of the HBM model, and the maternal practice checklist. Finally, the data were analyzed.
Results: The mean age of subjects was 26.48 ±4.77 and the mean gestational age was 14.88 ±5.59 weeks. The highest level of education for pregnant women (47.1%) was a diploma. The mean practice score was 72.9 and among the structures, the highest and the lowest score was obtained for perceived susceptibility(83.25) and barriers(59.16), respectively. To predict the hehavior by using the HBM structures, regression analysis showed that of the studied variables, the external practice guides, perceived susceptibililty, and barriers are as behavior predictive predictive agents that totally, predicted 26% (R2=0.265) of behavior changes.
Conclusion: According to the results of the study, the design of educational interventions should be based on the external practice guides, perceived susceptibility and barriers structures as the most important predictors of maternal behavior.
Davarkhah Rabbani, Mohammad Reza Rezaeimofrad, Ashraf Mazaheri, Mohadeseh Mosayebi,
Volume 18, Issue 9 (12-2015)
Abstract
Background: The growth of population, improvement of quality of life and the development of industries have led to increase in the rate of urban and industrial waste. As the leachate of the waste has a lot of pollution, influences harmfully human health and the environment. Researches have shown that the advanced oxidation processes (AOPs) such as Fenton and Fenton-related processes can reduce chemical oxygen demand (COD) of the leachate effectively.
Materials and Methods: In this paper, the rate of decrease in the total suspended solids (TSS) of leachate from a composting facility in Isfahan was studied using electrochemical, Fenton and electro-Fenton processes. These processes were conducted at reaction times 0, 20, 40, and 60 minutes, with the currents of 0.5, 1 and 1.5A and hydrogen peroxide dosages 1000, 2000, and 3000 mg/L.
Results: In the three mentioned processes, the most TSS removal was observed at the first 40 minute of the reactions. Among the studied processes, the electro-Fenton removed up to 92.4% of the TSS and was recognized as the most effective method. Furthermore, electrochemical and Fenton removed 41.7% and 60.3% of the TSS, respectively.
Conclusion: Therefore, decreasing the TSS of leachate could change its qualities and reduce the leachate pollution and the adverse environmental effects.
Khadijeh Khosravi, Nader Zarinfar, Ehsanollah Ghaznavi-Rad, Ghasem Mosayebi,
Volume 19, Issue 2 (5-2016)
Abstract
Background: Brucella is an intracellular bacterium that causes chronic infection in humans and domestic animals. The underlying mechanisms that cause prolonged illness are complex and not fully understood. Immune responses may have an important role in the chronicity of infection. Here, we evaluated the lymphocyte proliferation responses in patients with chronic and acute brucellosis.
Materials and Methods: This descriptive - analytical study was performed on 22 patients with acute brucellosis, 21 patients with chronic brucellosis and 21 healthy people with the similar age, sex and genetic background as control group. Peripheral lymphocytes were isolated using Ficoll and the cellular proliferation was quantified in presence of antigen and phytohemaglutinin-A by MTT method.
Results: The brucella antigen-specific stimulation index in patients with chronic brucellosis was significantly lower than the acute brucellosis patients (p=0.001). Also, stimulating the lymphocytes with phytohemaglutinin-A has shown that proliferative response in patients with chronic brucellosis was lower than the other groups (p=0.04).
Conclusion: The results indicated that chronic brucellosis inhibits lymphocyte proliferation. This inhibition of lymphocyte proliferation may be due to the induction of anergy.
Parisa Nourbakhsh, Rezvan Merzaei, Fatemeh Yeganeh-Fard, Ghasem Mosayebi,
Volume 19, Issue 5 (8-2016)
Abstract
Background: Breast cancer is one of the major causes of cancer-related death and the most common solid malignancy in women worldwide. Chemerin as a new adipokine has an inflammatory activity that initiates inflammation via chemotaxis of immature DCs and macrophages. This study aimed to evaluate the level of chemerin in patients with breast cancer.
Materials and Methods: In this cross-sectional study that was performed as a case-control study, we enrolled 45 patients with breast cancer in Vali-asr hospital from June to December 2015 (age range, 18-60 years) and 40 healthy volunteers as a control group (age range, 22-56 years). The patients with invasive breast were selected before mastectomy. The level of serum chemerin was measured by ELISA kit.
Results: The results showed that the mean serum chemerin level in the breast cancer patients (1536 ±608 ng/L) was significantly lower than the normal individuals (1919±544 ng/L),(p=0.04). There is no correlation between the level of chemerin with body weight, stage of disease, duration of disease and the number of white and red blood cells.
Conclusion: With due attention to the inflammatory role of chemerin, decreasing the serum chemerin level in patients with breast cancer may be related to the consumption of chemerin in the inflammatory responses or immunosuppression by tumor. The low level of chemrerin may be suitable for tumor growth and progression.
Ali Ghazavi, Ali Ganji, Mohsen Khaki, Ghasem Mosayebi,
Volume 21, Issue 5 (10-2018)
Abstract
Background and Aim: There are two philosophies about the origin of the immune system, defense and homeostasis. In this article, the existential philosophy of the immune system was reviewed, in which the defense role is only part of its homeostasis functions.
Materials and Methods: The Medline was searched for terms such as “Homeostasis”، “Immunculus”، “Natural autoantibody” “Evaluation” and “Immune system”.
Findings: Hemostasis is the most important part of immune regulation and the existential philosophy of this system, so that its defensive role is only part of homeostasis functions. Natural antibodies play this role as immune reflection by showing qualitative and quantitative changes in the antigenic composition of the body. Therefore, the level of natural antibodies can also serve as a prognostic marker.
Conclusion: Although many improvements have been made in explaining the hemostatic role of the immune system, many studies have to be conducted to identify its particular aspects.
Ali Ganji, Amir Mohammad Saeedi, Ali Ghazavi, Ghasem Mosayebi,
Volume 22, Issue 4 (9-2019)
Abstract
Cancer is one of the leading causes of death worldwide. Thus, it is important to find newer, more selective, and more effective therapies for this disease. One of these methods that have attracted many researchers is using anticancer peptides regarding their specificity, lower side effects, and higher effectiveness on the cancer cells. One type of anticancer peptides is antimicrobial peptides. Although they have already been studied and introduced as potential agents to fight infectious diseases, only recently they have been used as a new way of cancer treatment. For decades, antimicrobial peptides have been considered a component of the native immune system; however, they can also be used as anticancer peptides due to their mechanisms and properties. This new therapeutic approach can provide a promising pathway for optimal cancer treatment with fewer side effects.
Mahdieh Mondanizadeh, Niloofar Moradi, Razieh Amini, Behzad Khansarinejad, Ghasem Mosayebi,
Volume 22, Issue 5 (11-2019)
Abstract
Background and Aim Chronic Lymphocytic Leukemia (CLL) is the most commonly occurring leukemia in adults, accounting for about 30-25% of total leukemia. One of the important etiological causes of this leukemia is the disruption of the Nuclear Factor Kappa B (NF-kB) signaling pathway. The two proteins of Apoptosis-Inducing Ligand (APRIL) and B-Cell Activating Factor (BAFF) play a role in the pathogenesis of this leukemia by affecting the NF-kB signaling pathway. In this study, due to the effect of miRNAs in regulating many cellular processes, the prediction of the prominent miRNAs targeting APRIL and BAFF transcripts in B-cell CLL patients was evaluated using specific and different bioinformatics programs.
Methods & Materials Afterwards retrieving the sequences of APRIL and BAFF proteins from the NCBI website, by using several programs including miRanda, TargetScan, miRWalk, DIANA and miRDB with different algorithms, the prediction of miRNAs targeting these genes was investigated.
Ethical Considerations This study was approved by the Research Ethics Committee of Arak University of Medical Sciences.
Results Based on the scoring system of bioinformatics programs, “hsa-miR-145-5p” and “hsa-miR-185-5p” were identified as miRNAs targeting APRIL gene, while “hsa-miR-424” and “hsa-miR-497”were miRNAs targeting BAFF gene. They were suggested for the practical studies in future.
Conclusion Based on the important role of APRIL and BAFF genes in the normal process of cell death and B-cell evolution, it seems that the mi-RNAs predicted by bioinformatics programs using different algorithms can be used as a diagnostic molecular biomarker to identify B-cell CLL patients.
Mohsen Khaki, Hamid Abtahi, Ghasem Mosayebi,
Volume 22, Issue 6 (February & March 2020)
Abstract
Background and Aim: The most important problem in the production of recombinant proteins in prokaryotic cells is the disruption of the function of these proteins due to their altered natural structure. The aim of present study is to identify the best chemicals dialysis buffer additives in order to improve the protein structure of recombinant Vascular Endothelial Growth Factor (VEGF)
Methods & Materials: In this experimental study, different chemicals additives were selected using relevant software. After adding these additives to the recombinant VEGF dialysis buffer, their effect on the refolding of recombinant proteins and the differentiation of mesenchymal stem cells into endothelial cells was assessed by flow cytometry method.
Ethical Considerations: This study obtained its ethical approval from the Research Ethics Committee of Arak University of Medical Sciences (Code: ARAKMU. REC.1394.199).
Results: The results showed that the addition of arginine, cysteine and dithiothreitol (DTT) to dialysis buffer increases the differentiation of mesenchymal stem cells into endothelial cells. With the presence of sodium chloride (NaCl), cysteine, arginine and DTT in treated cells, the rate of specific Cluster Differentiation (CD) markers of endothelial cell (CD31/144) was at the highest level.
Conclusion: Adding cysteine, arginine, DTT and NaCl to the dialysis buffer of recombinant VEGF had the greatest effect on the mesenchymal cell differentiation into endothelial cells.
Ensieh Seif Amirhoseiny, Ali Ganji, Ghasem Mosayebi, Ali Ghazavi,
Volume 23, Issue 1 (April & May 2020)
Abstract
Background and Aim: Fennel with phytoestrogenic, antioxidant and anti-diabetic properties is used in traditional Iranian medicine to treat Polycystic Ovarian Syndrome (PCOS). Chronic inflammation is a new proposed mechanism in PCOS pathogenesis that suggests the role of immune system in this disease. Due to the main role of Treg cells in maintaining immune homeostasis, their impaired function, along with increased production of inflammatory compounds such as nitric oxide, can lead to persistent inflammation.
Methods & Materials: In this experimental study, 18 BALB/c mice were randomly divided into three groups including control group, untreated PCOS-induced group, and fennel-treated group. The control group received 0.1 ml sesame oil for 21 days. PCOS was induced by subcutaneous injection of Dehydroepiandrosterone (60 mg/kg/day) plus 0.1 mL sesame oil for 21 days. Mice in third group were treated by intraperitoneal injection of 500 mg/kg/day fennel extract after 21 days. The regulatory of T cells (Tregs) were analyzed by flow cytometry method and nitric oxide level determination was performed by Griess Reaction method. Ferric Reducing/Antioxidant Power (FRAP) assay was used to measure the total antioxidant power.
Ethical Considerations: This study was approved by the Ethical Committee of Arak University of Medical Sciences (Code: IR.ARAKMU.REC.1397.312).
Results: The estrous cycle was normalized after treatment with fennel. Fennel significantly reduced the number of ovarian cysts. Nitric oxide concentration decreased significantly while FRAP concentrations did not significantly alter. The number of Treg cells was also increased significantly.
Conclusion: Due to the role of immune system in the incidence of inflammation, concurrent modulation of both immune and endocrine systems may open new horizons in the design of new therapeutic interventions for PCOS.
Ali Ganji, Ghasem Mosayebi, Mohsen Khaki, Ali Ghazavi,
Volume 23, Issue 1 (April & May 2020)
Abstract
Background and Aim: COVID-19, a viral respiratory illness. In this review, we discuss issues such as the genetic structure, pathogenic mechanism, clinical characteristics, diagnosis, treatment, and prevention of COVID-19 based on the latest findings.
Materials and Methods: The Medline was searched for terms such as “Coronavirus” ،“ COVID-19” and “Respiratory infection”.
Findings: In December 2019, the unprecedented outbreak of pneumonia with unknown etiology began in Wuhan, Hubei Province, China. The cause was a new coronavirus, named COVID-19 by the WHO. This virus has high transmissibility and affects the lower respiratory tract. Research into the mechanism and treatment of COVID-19 is under way, and new information is being provided every day, some of which are contradictory. COVID-19 is a serious and dangerous infectious disease and a significant threat to the health of the world's population, and should be prevented from spreading rapidly.
Conclusion: Because the exact mechanisms of the disease have not been fully elucidated and specific drug or vaccine have not yet been developed for it, control is mainly based on the interruption of the transmission cycle.
Zahra Bahramnezhad, Ali Ghazavi, Ali Ganji, Ghasem Mosayebi,
Volume 24, Issue 3 (August & September 2021)
Abstract
Background and Aim: Medicinal plants contain various biological compounds, including phenols, flavonoids, and terpenoids, with anti-radical and anti-inflammatory activities that affect human health and improve life. Considering the numerous beneficial effects of Tanacetum Parthenium and Satureja Montana and the lack of full antioxidant capacity and anti-inflammatory effects of their combination, we decided to combine these two plants’ anti-inflammatory and antioxidant effects on induced acute inflammation in BALB/c mice.
Methods & Materials: In the present study, mice received the extracts of T. Parthenium and S. Montana and their combination by gavage for 14 consecutive days. Then, to induce acute inflammation, thioglycollate was injected intraperitoneally to all groups. FRAP and Grease tests were used to evaluate the total antioxidant capacity and serum nitric oxide concentration, respectively. One-way ANOVA analyzed the results.
Ethical Considerations: This study was approved by the Research Ethics Committee of Arak University of Medical Sciences (Code: REC.1398.021).
Results: The results showed that the combination of the extracts (P=0.006) and Satureja Montana (P=0.021) led to a significant increase in total antioxidant activity compared to the control group. Also, according to the results of grease test, Satureja montana (P=0.04), Tanacetum parthenium (P=0.034), and their combination (P=0.003) significantly reduced serum nitric oxide production compared to the control group.
Conclusion: The present study shows the synergistic effect of the combined extracts to increase their total antioxidant capacity and anti-inflammatory.
Maryam Bahrami, Ali Ghazavi, Ali Ganji, Ghasem Mosayebi,
Volume 24, Issue 3 (August & September 2021)
Abstract
Background and Aim: In addition to free radicals such as Nitric Oxide (NO), inflammation is one of the most important pathophysiological causes of peritonitis. Over thousands of years, Nigella Sativa (NS) and Silybum Marianum (SM) are two plants known for their anti-oxidant and anti-inflammatory properties. However, the effect of its compound is unclear. Thus, in this study, we evaluated the anti-inflammatory effect of NS and SM extracts and their combination on inflammatory diseases like thioglycollate peritoneal.
Methods & Materials: Alcoholic extracts of SM and NS were obtained by the soxhlet method. Male Balb/C mice were divided into 5 groups and gavage orally for 14 days with SM, NS, the mixture of extracts of these two, DMSO 30% as the control group, and dexamethasone as the positive control group. The safety profile and acute toxicity in mice were assessed. On day 10, acute peritonitis was induced by thioglycollate 3%. Finally, the total anti-oxidant power and NO concentration were measured by FRAP and Griess method, respectively, in the serum of treated mice.
Ethical Considerations: All experimental process was performed following the guidelines according to the Animal Ethics Committee of Arak University of Medical Sciences (IR.ARAKMU.REC.1397.359).
Results: Acute toxicity test showed no significant changes in weight and physical appearance of the mice. However, the extract and their mixture decreased NO level significantly (P=0.000) in serum. Also, the mixture significantly increased total anti-oxidant power (P=0.015).
Conclusion: Results showed that the SM and NS extract mixture demonstrated anti-inflammatory activity, inhibiting inflammatory mediators such as NO and increasing anti-oxidant power, thus supporting its therapeutic potential in slowing down inflammatory processes in inflammation disorders.
Mahdi Mosayebi, Reza Hajhossein, Mona Moghadasi,
Volume 26, Issue 6 (February & March 2024)
Abstract
Introduction: Fasciolosis is one of the most important parasitic disease common among both humans and livestock. That causes several health problems and economical losses in different parts of Iran. In order to control this disease, chemicals are used, which in some cases lead to resistance to the used substances and cause environmental problems. In this study, the effectiveness of the ethanolic extract of Lippia citriodora on the eggs of Fasciola hepatica is investigated.
Methods: Fasciola hepatica eggs were obtained from the liver and gall bladders of naturally infected sheep and kept at 4˚C until use. Ethanol extract of Lippia citriodora kunth was provided from dried leaves. The eggs were exposed to Different concentration of extract. Concentrations of Lippia citriodora extract (10, 20, 30, 40, 50, 75 and 100%). Control group eggs were placed in chlorine-free water. Egg and embryo development stages to complete and release miracidia were examined every day. Ethical considerations: This study was conducted after receiving the code of ethics (93-175-13) from the University Research Council.
Results: In concentration of 10 percent Lippia citriodora, miracidia was formed after 16 days in the egg. With increasing of concentration percentage of the extract, time of miracidia formation in eggs also rose. The time of miracidia formation between cases and control group is significantly different (P < 0.05).
Conclusions: Our study shows that extract of Lippia citriodora is an effective inhibiting factor against development of Fasciola eggs.
Rouhollah Hemmati Bushehri, Mahmoud Reza Jaafari, Ghasem Mosayebi, Ali Ghazavi, Ali Ganji,
Volume 27, Issue 6 (1-2025)
Abstract
Introduction: Nowadays, plant-derived compound is widely used in medical treatment as complementary therapies. Rosemary is one of these plants that have anti-angiogenic and anti-tumor effects. However, low solubility and low bioavailability lead to limited use of this compound. To overcome this limitation, the use of liposomes is beneficial. So, this study aimed to prepare liposomes containing rosemary alcoholic extract to obtain the best formulation with best properties to use in the antitumor study.
Methods: Liposomes were prepared by Thin-Film-Hydration method in different ratios of rosemary extract, hydrogenated-soybean-phosphatidylcholine (HSPC), Cholesterol, and mPEG2000 (F1-F6). The characterization of nanoliposomes includes size, PDI (Poly dispersity index), zeta potential, encapsulation efficiency, and release rate was measured.
Results: The results showed that the average particle size and zeta potential were 113.4 nm and -10.8 mV, respectively. Encapsulation efficiency showed a range of 60- 90%. The average release rate of the extract was about 60%. Also, the results showed that the F1 formulation was transparent, had appropriate properties, and did not precipitate. F2 formulation was not transparent. F3-F4 formulations were precipitated, and F5-F6 formulations did not achieve the appropriate size.
Conclusions: This study proved that F1 formulation in a ratio of 55:30:5 HSPC/chol/mPEG2000 is the best formulation for the preparation of nanoliposomes containing rosemary alcoholic extract to achieve the best properties for use in cancer studies.
