Ali Ganji, Iman Farahani, Mohammad Reza Bayatiani, Ghasem Mosayebi, Mohammad Hasan Sakhaie,
Volume 28, Issue 6 (1-2026)
Abstract
Introduction: Experimental Autoimmune Encephalomyelitis (EAE) is an immune-mediated inflammatory model of multiple sclerosis that induces axonal loss, demyelination and motor disabilities. Electromagnetic fields (EMFs) possess multiple biological capabilities that affect the nervous system. The aim of the present study is to investigate the beneficial effect of low-intensity electromagnetic field on clinical symptoms, histological structure and gene expression in the cerebral cortex.
Methods: Forty-eight female C57BL/6 mice were used for this study. The EAE model was induced by immunization with myelin oligodendrocyte glycoprotein (MOG). Subsequently, the animals were exposed to EMFs (1 mT, 50 Hz) daily over a period of two weeks. At the end of the experiments, body weight, clinical score (paralysis score), and lymphocyte infiltration of the cortex were evaluated in the experimental groups. Moreover, the mRNA expression levels of Bcl2, BDNF, and Nrf2 were also studied using quantitative polymerase chain reaction (qPCR).
Results: EMF exposure significantly reduced the clinical symptom scores and decreased lymphocytic infiltration. The applied EMF also upregulated the expression of Bcl2, BDNF, and Nrf2 in the cerebral cortex compared to the EAE model.
Conclusions: Overall, the present study demonstrated that electromagnetic fields, as a complementary therapy, exert significant neuroprotective properties in EAE by inhibiting oxidative stress and reducing neural tissue inflammation.
