Background: Approximately, 50% of male infertility causes have remained unknown. It seems that genetic disorders may lead to many cases of idiopathic infertility. XRCC1 ( X-ray Repair Cross Complementing group 1) acts as a scaffolding protein in the base excision repair (BER) and single strand break repair (SSBR). Single nucleotide polymorphisms (SNP) of XRCC1 may influence DNA repair capacity. Thus, they had been considered as a risk factor for infertility. XRCC1 Arg399Gln polymorphism was located on p rotected domain , BRCT1 . The aim of this study was to explore the p ossibility of association between XRCC1 Arg399Gln polymorphism and susceptibility to idiopathic male infertility.

  Materials and Methods: In this case-control study, the genotype and allele frequencies of Arg399Gln polymorphism were examined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) on a Guilanian population consisting of 144 men with idiopathic infertility and 166 healthy men. Statistical analysis was performed using the MedCalc 12 software.

  Results: According to our results, compared with Arg/Arg genotype, the Arg/Gln , Gln/Gln and Arg/Gln + Gln/Gln genotypes showed a significant association with an increased risk of idiopathic male infertility ( OR=4.19 95%CI 2.37-7.41, p<0.0001 ), (OR=3.42 95%CI 1.50-7.81, p<0.0034), (OR=4.06 95%CI 2.32-7.09, P<0.0001) , respectively. In addition, the Gln allele frequency in patients was significantly higher than that in controls(p=0.0004).

  Conclusion: In total, Arg399Gln polymorphism of XRCC1 gene can be associated with male infertility and Gln allele might be a risk factor of idiopathic male infertility in in this sample population. Larger population and different ethnicities should be studied to achieve a definitive conclusion.

Background: The purpose of this study was to determine the effectiveness of dialectical behavior therapy in reducinng expulsive anger.

Materials and Methods: This is a pre-test and post-test semi-experimental study with control group. The study population included 32 patients with expulsive anger that reffered to Amir kabir remedial education center based on psychiatrist diagnosis and were randomely divided into case (dialectical behavior therapy) and control group. The case group was educated the dialectical behavior therapy skills for 16 sessions from 2 until 2.5 hours at teamly. To collect data, the patients filled Eysenk expulsive questionnaire before and after intervention. Considering no necessary assumptions were fulfilled to conduct the multivariate analysis of covariance and variance, so, the univariate analysis of variance was used.

Results: The results of variance analysis proved that dialectical behavior therapy significantly reduced expulsive angers.

Conclusion: With due attention to the results, it can be said that dialectical behavior therapy leads to reducing expulsive angers in treated patients, thus, this method can be included in interventional programs for target groups in psychotherapy centers. Aslo, it helps to improve the life of patients by reducing expulsive angers.

Background: One of the important adaptations that occurs after exercise is increased capillary density or angiogenesis. Vascular endothelial growth factor, has a mitogenic role for endothelial cells and acts as an important intermediator in the process of angiogenesis. The aim of this study was to compare the effects of two kind of endurance training on vascular endothelial growth factor gene expression in healthy male rats.

Materials and Methods: In this laboratory experimental study, 18 male Wistar rats at the age of eight weeks, with an average weight of 210/5± 9/77g were  selected and randomely divided into three groups (control (n=6), ET (n=6) and HIIT (n=6)). Aerobic continuous training was performed 5 days a week, totally in eight weeks for 30 minutes with 70-75% VO2max and high intensity interval training consisted of three periods (four minutes with 90 to100% VO2max and two minutes with 50 to 60% VO2max). Vascular endothelial growth factor gene expression was measured by real time-PCR technique. To determine the significance of variables between these groups, one-way ANOVA and Tukey's post hoc tests were used.

Results: The results showed that the gene expression levels of vascular endothelial growth factor were increased significantly (p=0/006, F=7/243) in intense aerobic continuous and interval training groups compared to control group. Changes in exercise groups compared with each other were not significant.

Conclusion: According to the results of this study, increased levels of vascular endothelial growth factor gene expression in both training groups caused pro-angiogenic function in endothelial cells and an increase in ratsVO2max following eight weeks training may be due to increased angiogenesis process. High intensity interval training may cause faster adaptations in the body of organism than aerobic continuous training.

Background: Under-nutrition has multiple negative impacts on child's growth and development and it has a high prevalence among hospitalized children. Assessment of the food insecurity status is one of the most important indicators of malnutrition among communities, therefore the aim of the current study is to evaluate the prevalence of food insecurity and its social, economical and nutritional determinants among children hospitalized in Tabriz pediatric hospital.

Materials and Methods: The current cross-sectional study was carried out among 236 hospitalized children in Tabriz pediatric hospital. Demographic information including age, weight, height, household size, child order and household income were gathered by face to face interviews with parents. Food security was assessed by a Household Food Security questionnaire. Statistical analysis was performed by Excel 2007, Epi-info and IBM SPSS Statistics 21 software.

Results: The prevalence of food insecurity among hospitalized children in Tabriz pediatric hospital was 44.9%, while 26.3% and 18.6% had moderately and severely food insecurity, respectively. Its prevalence in females was more than in males. Low household income, low educational attainment of parents, rural residency and household size were potent determinants of food insecurity(p<0.05). All indices of nutritional status in food insecure group were lower than in secure group. Although this difference did not achieved significant threshold.

Conclusion: In the current study, a high prevalence of food insecurity in hospitalized children in hospital has been reported. Therefore the need for suitable interventions to reduce its prevalence has been warranted.

Background: Dendrimers are macromolecules with regular three-dimensional structure that have many branches. The aim of this study was to investigate the fficiency of Nano Polyamidoamine–G5 (NPAMAM-G5) dendrimers in removal of Escherichia coli, Klebsiella oxytoca, Pseudomonas aeruginosa, Proteus mirabilis and Staphylococcus aureus from aqueous solution.

Materials and Methods: In this experimental study, initially, dilution of 10³ CFU/ml was prepared from each strain of bacteria. Then, different concentrations of dendrimers (0.025, 0.25, 2.5 and 25 µg/ml) in the ambient temperature (23-25°C) was added to water. In order to determine the efficiency of dendrimers in removal of bacteria, samples were taken at different times (0, 10, 20, 30, 50 and 60 min) and were cultured on nutrient agar medium. Samples were incubated for 24 hours at 37 ° C and then the number of colonies was counted.

Results: There is a direct relation between antibacterial properties of dendrimers in aqueous solution and increasing the dendrimer concentration and contact time. At a concentration of 25 μg/ml and 60 minutes, all kinds of bacteria except Staphylococcus aureus, and at 30 minutes, E. coli and Klebsiella bacteria, were removed by 100% The concentration of 2.5 μg/ml at 60 minutes of  bacteria, Escherichia coli, Klebsiella and Proteus mirabilis, 100% Were removed. All concentrations of dendrimer at different times were reduced bacteria.

Conclusion: Results of this study indicated that NPAMAM–G5 dendrimer is able to remove Escherichia coli, Klebsiella oxytoca, Pseudomonas aeruginosa, Proteus mirabilis and Staphylococcus aureus from aqueous solution.

Background: Influenza A viruses are globally important respiratory pathogens which cause a high degree of morbidity and mortality during annual epidemics. M2 protein which expressed on the viral surface facilitates virus entry to the host cells. The extracellular domain of M2 protein (M2e) consists of N-terminal 24 residue which shows remarkable conservation among all subtypes of influenza A viruses. In this study, we evaluated the immunogenicity of three tandem repeats of M2e along with different adjuvants in BALB/C mice model.

Materials and Methods: Recombinant protein (3M2e) was expressed in Escherichia coli and purified. Six weeks old BALB/c mice were immunized interdermally with three doses of 3M2e alone or supplemented with Alum/CpG motif as adjuvant. Control group was injected with PBS. Two weeks after the last immunization, specific anti-M2 was measured using ELISA method and finally mice were challenged with one lethal dose (LD90) of PR8 virus.

Results: The results showed that 3M2e can induce specific antibody alone. However, 3M2e protein supplemented with Alum-CpG induced higher level of specific antibodies, so that, there was a significant difference with 3M2e group (p<0.05). Anti-M2 antibodies mostly consisted of IgG2a subclass which considered as activity index of TH1 Cells. Moreover, this group showed enhanced protection against wild-type virus (survival rate=60%).

Conclusion: Applying Alum-CpG as a complex adjuvant may play a crucial role in integrating innate and acquisitive immunity. We increased density of M2e in combination with complex adjuvant and showed that this vaccine induced power immune responses and semi-protected mice against lethal challenge.

Background: Brucella is an intracellular bacterium that causes chronic infection in humans and domestic animals. The underlying mechanisms that cause prolonged illness are complex and not fully understood. Immune responses may have an important role in the chronicity of infection. Here, we evaluated the lymphocyte proliferation responses in patients with chronic and acute brucellosis.

Materials and Methods: This descriptive - analytical study was performed on 22 patients with acute brucellosis, 21 patients with chronic brucellosis and 21 healthy people with the similar age, sex and genetic background as control group. Peripheral lymphocytes were isolated using Ficoll and the cellular proliferation was quantified in presence of antigen and phytohemaglutinin-A by MTT method.

Results: The brucella antigen-specific stimulation index in patients with chronic brucellosis was significantly lower than the acute brucellosis patients (p=0.001). Also, stimulating the lymphocytes with phytohemaglutinin-A has shown that proliferative response in patients with chronic brucellosis was lower than the other groups (p=0.04).

Conclusion: The results indicated that chronic brucellosis inhibits lymphocyte proliferation. This inhibition of lymphocyte proliferation may be due to the induction of anergy.

Background: Chronic kidney disease as an important risk factor is associated with some disorders which are key causes of death and disability in older people. Therefore, the aim of this study was to assess the combined effect of regular aerobic exercise with garlic extract on renal apoptosis regulatory factors in aged rats with chronic kidney disease.

Materials and Methods: In this experimental research, 42 aged male Wistar rats(48-52 weeks) were selected and randomly divided into 6 groups: control, doxorubicin, doxorubicin-salin, doxorubicin- garlic, doxorubicin - exercise, doxorubicin –garlic-exercise(combined). Chronic kidney disease was induced by a single subcutaneous injection 8.5 mg/kg of doxorubicin. Swimming training was programmed 3 days /week, 30 min/day for 8 weeks. Both the doxorubicin garlic and combined groups with garlic extract were administered by garlic gavage at a dose of 2.5 g/kg. The renal Bax and Bcl-2 levels were evaluated by ELIZA method. A one-way analysis of variance was used to data analysis (p<0.05).

Results: The results showed that induced chronic kidney disease was associated with a significant increase on Bax and a decrease on Bcl-2 in aged rats. Also, 8 weeks swimming training, garlic supplementation and the combined intervention significantly reversed these changes. Furthermore, no significant difference have been observed in the effect of these interventions on Bax and Bcl-2 in aged rats with chronic kidney disease.

Conclusion: It seems that the use of non-pharmacological treatment methods such as exercise training, garlic extract supplement, and combination of the both interventions may be effective in reducing apoptosis resulted from chronic kidney disease in aged rats.

Background: Considering the high prevalence of sexual dysfunction in females with OCD and its impact on marital satisfaction, the purpose of this study was to investigate the relationship between sexual function and marital satisfaction in a group of female OCD patients.

Materials and Methods: In this cross-sectional study, 36 females with OCD, referred to the clinic of Roozbeh Psychiatric Hospital (related to Tehran University of Medical Sciences) and 3 private offices in Tehran, were selected by random sampling and completed questionnaires including: demographic FSFI, BDI-II, MOCI, OCI-R and researcher-developed marital satisfaction questionnaire.

Results: 80.6% of women reported sexual dysfunction. The results of studying sexual function questionnaire subscales was as following: 50 % low sexual desire, 58.3 % low sexual arousal, 36.1 % decreased lubrication, 44 % orgasmic disorder, 52.8 % sexual pain disorder and 41.7 % sexual dissatisfaction. Furthermore, statistically significant correlation was found between sexual dysfunction (total score and arousal, lubrication, pain and satisfaction subscales) and marital dissatisfaction. Studying the relation between obession(MOCI questionnaire) and marital satisfaction revealed that the correlation between washing subscale of MOCI and marital dissatisfaction was also significant while there were not any significant correlation between obsession (OCJ-R, MOCI) and sexual function (FSFI).

Conclusion: High prevalence of sexual dysfunction in OCD patients, significant relationship between obsession and marital satisfaction, and sexual function and marital satisfaction could demonstrate relationship between sexual function, OCD and marital satisfaction.

Background: Determining the level of manual ability of children with cerebral palsy has significant role in scheduling care and providing supportive services by organizations such as the a social welfare office. Manual Ability Classification System (MACS) is responsible for this critical matter. In Mazandaran Province, the prediction of manual ability is intuitive and is done without tools. This study aimed to investigate the consistency between operational therapists prediction of future manual function of children with cerebral palsy and MACS scale.

Materials and Methods: This study was designed as a cross sectional trial. The study population consisted of 12 occupational therapists working in Mazandaran’s rehabilitation center under the social welfare office. Firstly, occupational therapists chose 100 children with cerebral palsy and classified their future manual ability into five levels according to their prediction. Then the researcher classified the children based on MACS scale. Finally, the amount of prediction consistency between therapists and researcher was statisticaly analyzed.

Results: The weighted kappa coefficients of MACS scale were 0.671 in first level and 0.747 in fifth level that show good agreement in these two levels. This coefficient was 0.417 in third level and 0.444 in forth level that shows fair agreement. The weighted kappa coefficient was 0.358 in second level that indicates slight agreement.

Conclusion: With prediction consistency between operational therapists and researcher, MACS is used as a suitable means for classifying the level of manual ability in children with cerebral palsy and predicting their needs to adaptive and auxiliary devices by occupational therapists in rehabilitation centers and social welfare offices.

Background: The renin-angiotensin system(RAS) has a major role in development of diabetic nephropathy and blocking of RAS by inhibitors and blocking of angiotesin receptors is standard treatment for preventing kidney disease and proteinuria. It is reported that VIT-D analogues are able to suppress renin exertion and improve proteinuria. The aim of this study is to evaluate the effect of VitD analogue (calitriol) on reducing proteinuria in patients with diabetic nephropathy.

Materials and Methods: In this clinical trial study, 132 eligible patients that had diabetic nephropathy and hadn’t vit D deficiency were selected. The patients were divided into two equal groups. First group received the combination of losartan 25mg twice daily and calcitriol 0.25mg and second group received losartan 25 mg twice daly alone for 3 months. The FBS, lipid profile, ESR-CRP BUN, Cr, HbA1c, Ca, P, and 24 hours urine protein were evaluated in all patients at beginning and end of study and the results were statistcally compared.

Results: The 24-hour urine protein in losartan and calitriol group was improved compared to losartan. This difference was statistically significant (p=0.003). As well as, in kidney function (BUN, Cr) in the losartan and calcitriol group compared to losartan alone was significantly improved(p<0.05).

Conclusion: Combination of calcitriol with angiotesin receptor blockers(ARBs) is more effective than ARBs alone in improvement of proteinuria and real function.

Background: Angiogenesis is an important biological processes of new blood vessels in many pathological stages of development and embryo development occurs and a complex and dynamic phenomenon that is needed for development and other physiological processes. This study aimed to investigate the effect of alcoholic Ocimum basilicum leaf extract on angiogenesis chick chorioallantoic membrane is done.

Materials and Methods: In this experimental study 40 Ross fertilized eggs were randomly divided into four groups: control, sham-exposed and experimental groups were divided. The second day of incubation the eggs window was opened. Eighth day of the alcoholic extract of basil doses of 50 and 150 mg/kg on chick chorioallantoic membrane was injected. On day 12, embryos length and weight and chorioallantoic membrane (CAM) was photographed by photostereomicroscope Then the numbers and lengths of vessels in special area on CAM were measured with Image J. analyzed through by t-test and ANOVA (P<0.05).

Results: The data does not show significant difference between embryos length and weight in sham compare to all experimental groups. In the study vessels number just with 150 mg/kg observed significant.

Conclusion: Alcoholic extract of basil is an increase in the number of vessels and in this sense the healing and growth processes associated with them as well as effective.

Abstract

Background: Infertility is described as a loss that this event is serious tension in life and it makes extreme trauma on couples. This research aimed to investigate the effect of group psychotherapy based on life quality on marriage intimacy of infertile women.

Materials and Methods: This research method was in semi-test methodology with pre-test, post- test and follow-up test plan with control group and statistical sample was 32 persons (16 persons in test group and 16 persons in control group) that they were chosen in available sampling mode from Royan center in Arak and both two groups were peer in research standards. Test group received training for 5 sessions in 90 minute. For collecting data, Bagarozzi marital intimacy questionnaire was used. Data were analyzed by analysis of covariance by using SPSS software.

Results: Results showed that life quality training affected significantly on marriage intimacy of infertile women and improved it. Significant difference between pre-test, post-test and follow-up average scores of test and control groups showed effectiveness of life quality training on infertile women ( p=0.003<0.05 ,f=27.57).

Conclusion: The research showed that group therapy of life quality plan affected on marital intimacy and improved it. Therefore, it is recommended as a supplement.

Abstract

Background: Nanoparticles are used in various applications due to unique mechanical and physicochemical properties such as their increased surface area to volume ratio and quantum effects. This study was designed to investigate the cytotoxic effects of zinc oxide nanopaticles on hematological and biochemical parameters BALB/c mice.

Materials and Methods: In this experimental study, 28 adult male mice BALB/c, were divided into four groups (one control group and three experimental groups). The mice in the experimental groups orally received Zinc Oxide nanoparticles with doses of 50, 100 and 300 mg/kg for 14 days. The control group received distillated water only. On 15th day, some hematological and biochemical parameters were studied on the blood samples collected.

Results: Results showed that Zinc Oxide nanoparticles cause changes in blood cells. In high concentration, nanoparticles increased some of factors such as white blood cells, hemoglubin, MCV and neutrophil and besides decreased amount of RBCs, pLTs, hematocrit, lymphocytes, glucose and kratenin significantly (p<0.05).

Conclusion: The findings showed that zinc oxide nanoparticles cause harmful effects due to the considerable variations in hematological and serum parameters in mice  in a dose-dependent way.

Abstract

Background: The present research aimed to measure the effectiveness of Eye Movement Desensitization and Reprocessing (EMDR) on reducing the severity of symptoms of post-traumatic Stress Disorder (PTSD) and Stuttering in a four-year-old child post-traumatic stress disorder in children is one of the disorders relating to trauma and stressful factors, also, stutterring or word fluency disorder is a nerve growth disorder.

Materials and Methods: This research design was conducted as a case study with a multiple baseline design. Participant in this research was a four-year- old child with Diagnostic Posttraumatic Stress Disorder and Psychogenic Stuttering. He had been placed under the intervention with EMDR at Shahid Birjand Counseling Center. The instruments used for this research included demographic researcher-made questionnaire, the third edition of stuttering severity test, the scale of parents’ report of post-traumatic Symptoms and child’s report of posttraumatic Symptoms. Data analysis was done through graphic and descriptive analysis. The data was collected as base line and during the treatment as well as after the treatment and follow-up (in terms of 3 and 24 months).

Results: Means percentage improvement (MPI) to reducing the severity of post-traumatic symptoms was achieved as %74.66 and it was %56.06 for reduction of the severity of stuttering and they continued to maintain in the follow-up period.

Conclusion: Results showed that EMDR method had affected on reduction of the severity of post-traumatic stress disorder symptoms and stuttering intensity.

Abstract

Background: Ventilator-associated pneumonia (VAP) is one of the most important causes of mortality in intubated traumatic patients admitted in ICU. The aim of current study was to determine correlation between blood products transfusion with incidence of VAP in traumatic patients under mechanical ventilation in ICU.

Materials and Methods: This study was a prospective descriptive-analytical study. Current study was carried out on traumatic patients under mechanical ventilation admitted to Imam Khomeini hospital ICU wards of Sari city, from September 2015 to September 2016. In addition to demographic data, patients' clinical information were daily recorded.

Results: Of total of 2304 patients admitted to ICU, 186 traumatic patients under mechanical ventilation longer than 48 hours were reviewed and analyzed. Among patients infected with VAP, 36 persons(87.8%) had transfusion of blood products before VAP incidence. Blood products transfusion (before and / or after 48 hours) had the highest adjusted risk ratio in the incidence of VAP (Odds ratio; OR=3.58). Also, amount of injected Packed Red Blood Cells and platelet were significantly higher in patients infected with VAP (p<0.005).

Conclusion: Considering remarkable increase in incidence of VAP following blood products transfusion and its correlation with injected PRBC and platelet, considering these modifiable risk factors for controlling and preventing the incidence of VAP in traumatic patients under mechanical ventilation in ICU is necessary and reasonable.

Abstract

Background: Bariatric surgery is the most effective intervention for treating severe obesity and patient's adherence to self-management behaviors is essential to reduce complications after surgery. The purpose of this study was to investigate the psychometric properties of BSSQ in Iranian population.

Materials and Methods: The statistical society was all of the obese patients that underwent laparoscopic obesity surgery in Shiraz Ghadir Mother & Child Hospital from December 2016 till June 2016, and 201 of them (149 females and 52 males) were selected by using available sampling method. They responded to demographic characteristics, post bariatric surgery self-management behaviors questionnaire and general and specific adherence scales. For validity, methods such as content validity, structural validity (factor analysis and correlation analysis), simultaneous validity were used. The reliability of the questionnaire was determined by using bisection and internal stability methods by Cronbach s alpha.

Results: According to the results of explatory factor analysis, sisx factors including eating behaviors, physical activity, fruits, vegitables, grain and protein intake, fluid intake and dumping syndrome management were elicited, that these 6 factors explained 61.54% of variance of self-management behaviors. Total score of correlation matrix BSSQ with GAS & SAS were 0.363 and 0.702. For reliability, the Cronbach's alpha coefficient of this instrument was found 0.90 and guttman split-half coefficient was 0.78.

Conclusion: Results show that BSSQ has an acceptable validity and reliability and it can be used for assessing the post bariatric surgery self-management behaviors in Iranian population.

Abstract

Background: Retrograde transport to the alpha motoneurons causes spinal degeneration. The neurotrophic factor (NT3) increases the number of myelinated axons in the dorsal root, leads to differentiation and survival of sensory neurons, parasympathetic motoneurons and prevents cell death. Lavender is a plant in the family Lamiaceae which is reported to have antioxidant, antispasmodic, diuretic, anti-asthmatic, refrigerant, and antipyretic effects. This study examined NT3 gene expression changes after sciatic nerve compression in rats, in the presence of Lavandula officinalis extract. 

Materials and Methods: Lavender Soxhlet hydroalcoholic extraction was prepared. 36 male Wistar rats were randomly divided into 3 groups including control, compression and treatment (compression group + hydroalcoholic extract of Lavender injections 75mg/kg) groups. In controls the muscle was opened without damage to gain access to the sciatic nerve. In compression and treatment groups, the sciatic nerve (right leg) was compressed. The extract was injected intraperitoneally in two occasions. A biopsy was taken from the spinal cord segments L4-L6 on day 28, total RNA was extracted and cDNA was synthesized and NT3 gene expression changes were analyzed by ANOVA test by using SPSS software. 

Results: The results showed that NT3 gene expression had a significant reduction in compression group compared to the control group (p<0.001) and it had a significant increase in treatment group compared with the compression group (p<0.001).

Conclusion: A significant increase in gene expression shows that Lavandula officinalis hydroalcoholic extract improves nerve regeneration via NT3 gene expression. 

Abstract

Background: Schizophrenia is a common mentaly disorder with high heritability and variable phenotype that they are placed in bipolar nervous disease family. Different genes are associated with this disease that among of them, DAOA gene plays a significant role. DAOA gene is located in the long arm of chromosome 13 and produces a protein called D-amino acid oxidase which is N - metyle D– aspartate (NMDA) receptor activator in the brain. The aim of this study was to investigate the association between DAOA gene polymorphisms and the risk of schizophrenia in Iranian population.

Materials and Methods: In this study, 100 patients with schizophrenia and 100 normal people as a control group were investigated. After genomic DNA extraction from blood samples, allele and genotype frequencies in patients and control group were determined by PCR-RFLP method. Data analysis was performed with Med Calc (ver 12) software.

Results: Frequency of GG, GA, AA genotypes in patients group was 18%, 42%, 40% and in normal group was 14%, 45%, and 41%, respectively. Statistical analysis revealed that there is a significant relationship between DAOA gene polymorphism with schizophrenia disease in the people under the age of 40.

Conclusion: According to the results of this study, the DAOA gene has significant association with schizophrenia in the Iranian population.

Abstract

Background: Uterine myomas are benign tumors of the uterus which are derived from smooth muscle cells of the myometrium. Genetic factors play a major role in the progression of the disease. One of the most important genes which have impression in the mechanism of formation of the myoma is epidermal growth factor receptor (EGFR) that plays a basic role in the process of cell growth, differentiation, proliferation and mitogenesis. The aim of this study is survey of EGFR gene common mutations in Iranian women with uterine myomas. In this test, the common mutations of the exon 21 and 19 in the EGFR gene were surveyed.

Materials and Methods: In this case-control study, 80 women with myoma and 80 healthy women were studied as control. For checking deletion mutation of the Exon 19 (rs121913438), the Tetra ARMS/PCR method has been used and also for checking point mutation of the exon 21(rs121434568), the ARMS/PCR method has been used and results of the experiments were analyzed via χ 2 test.

Results: The comparison of the genotypes frequency of exon 21 (TT, TG, GG) and exon 19 (WW, WD, DD) related to EGFR gene in two groups of patients and control with using statistical test respectively represents the significant difference (p=1.320e-16) and (p=3.053e-13) in the different genotypes frequency among the patients and control groups.

Conclusion: The results of research indicate a significant relationship between EGFR gene mutations in exon 19 and exon 21 and potential for myoma in the studied population.