Showing 41 results for Sari
Azam Fakhri, Soyar Sari, Ahmad Ebrahimi,
Volume 19, Issue 5 (8-2016)
Abstract
Background: Endometriosis disease is considered as a common disease dependent on androgen hormones. Androgens have different effects on endometrial growth. Androgen receptor as a signal transduction pathway could have a key role in regulating the process. Over hundreds of mutations leading to resistance gene function in androgen receptor (AR) has been recorded. Among these, the seuaence of CAG repeat in exon 1 had the largest share of studies related to the disease. The aim of the present study was to investigate the relationship between the AR gene CAG repeat variations in Iranian women with endometriosis.
Materials and Methods: In this study, 100 women with endometriosis and 100 healthy women as controls were selected. Exon 1 was amplified using PCR. Products of PCR were studied to determine CAG repeat variation in acrylamide gels.
Results: The number of CAG repeats in each group was determined between 18-26 repeats (mean±standard, 18.35±3.3).
Conclusion: According to the results of this study, no significant differences were found between the two groups of healthy women and women with endometriosis. The number of CAG repeats in each group was determined between 18-26 repeats which indicates a lack of relationship between CAG repeat diversity and endometriosis. According to the information, this study was conducted on patients with endometriosis in iran for the first time, although studies with larger sample are needed.
Pegah Parvaee, Mahdieh Mondanizadeh, Behzad Khansarinejad, Amir Nader Emami Razavi,
Volume 19, Issue 5 (8-2016)
Abstract
Background: Circulating microRNAs are promising biomarkers in diagnosis and assessment of cancerous patients. Quantitative Real-time PCR assay is a sensitive test for evaluating the levels of miRNAs expression. Nevertheless, there is no concurrence on selecting appropriate reference genes for qPCR analysis of miRNAs in circulation. Therefore, the current study aimed to select a suitable reference gene for normalizing the RT-qPCR assay results in plasma samples of patients with gastric cancer.
Materials and Methods: Based on previously published studies, three molecules SNORD47, U6 RNA, and miR-103 were selected as the candidate reference genes. After RNA extraction from plasma samples of 40 patients with gastric cancer and 40 healthy individuals, expression levels of these molecules were evaluated using Real-time PCR method.
Results: The results showed that the developed assays are able to diagnose their specified targets by a suitable linear range. By comparing patients and control groups, although the expression levels of miR-103 molecule were not equal between the two groups (p= 0.017), SNORD47 and U6 RNAs had similar expression levels. However, the variations of SNORD47 expression were lower that U6 RNA.
Conclusion: Based on the results of the current study, the SNORD47 molecule has a stable expression levels in plasma samples of patients with gastric cancer and normal individuals and can be used as an appropriate reference gene for normalizing the quantitative data of qPCR assay.
Hamta Ahmad, Ansari Jamshid, Bayati Zahra,
Volume 19, Issue 7 (10-2016)
Abstract
Background: Breast cancer is both the prevailing malignancy and the most common cause of cancer death among women. Many factors may play a role in the susceptibility to the breast cancer and Oxygen Free Radicals may be one of these. There are various known antioxidant systems against oxidative stress, including ParaoxonaseI. The aim of this study was to investigate the association between rs854560 polymorphism in the PON1 gene in patients with breast cancer.
Materials and Methods: We performed genotyping analysis using polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) assay in a case–control study of 83 confirmed breast cancer patients and 100 cancer-free controls in Markazi Province.
Results: In our study of the PON1 gene L55M polymorphism, the LL genotype was found in 2 (2.40%) patients, whereas the LM genotype was found in 69 (83.13%) patients. The MM genotype was present in 12 (14.45%) patients. In the control group, LL, LM and MM genotypes were found in 4 (4%), 81 (81%), and 15 (15%) subjects, respectively. There was no statistically significant difference between patient and control groups in terms of the PON1 gene L55M polymorphism (p= 0.825). Allele distributions were different but this difference did not reach statistical significance (p= 0.920).
Conclusion: We found no association between M55L polymorphism and breast cancer.
Neda Saleh Jafari , Farzad Zamanibarsari, Hamidreza Jamilian, Bahman Sadeghi Sede , Hamidreza Zafari,
Volume 19, Issue 7 (10-2016)
Abstract
Background: The definite treatment of indicated Hypertrophy of the tonsils is Tonsillectomy. The aim of this study was to compare the efficacy and necessity of diclofenac suppository and simultaneous prescription of acetaminophen alone in controlling and reducing pain and improving swallowing and satisfaction after surgery for tonsillectomy in children.
Materials and Methods: In this clinical trial, 180 children 7 to 14 years Tvnsykltvmy surgery were enrolled. Patients in an improbable way, easy and were divided into three groups of 60. Immediately after the surgery acetaminophen, diclofenac, or a combination of both was used. The cases of pain in the early hours, seventh, thirteenth and nineteenth after surgery were compared.
Results: There was a significant difference between the average pains of all groups in all hours (p<0.05). There was a significant difference between the groups from the point of view of side effects such as Nausea, Vomiting and Pyrexia (p<0.05), But there was no significant difference between the groups from the point of view of After Surgery Bleeding (p>0.05).
Conclusion: We can say that Rectal Diclofenac is a more effective medication for reducing pain after the Tonsillectomy surgery in contrast with Rectal Acetaminophen or a mixture of the two, which may cause the patients to use less Narcotics after the surgery.
Masomeh Barari, Soyar Sari, Ahmad Ebrahemi,
Volume 19, Issue 8 (11-2016)
Abstract
Abstract
Background: Hydatidiform Mole is a benign trophoblastic tumor is made of ectopic pregnancy. Abnormalities in the number or structure of chromosomes are causes of Hydatidiform Mole common numerical disorders resulted from proliferating repetitive sequences markers as called STR were studied in the region of chromosome X, Y, 13, 18 and 21. This study aimed to investigate chromosomal disorders prevalent in women with hydatidiform mole that was performed using QF-PCR techniques.
Materials and Methods: In this study, 50 women with hydatidiform mole and 80 healthy women as controls were selected. For studying the chromosomal abnormalities resulted of proliferating STR, Chromo Quant QF-PCR kit was used. Polymerase chain reaction was performed in PCR machine. Then electrophoresis was performed on Genetic Analyzer. Finally, amplified fragment were analyzed by Gene Marker software Statistical analysis was performed using SPSS version 19, and t-test. Data were expressed as mean ± SD. In this test, p <0.05 represents significant level between two groups.
Results: In this study ،of 50 samples, 8 samples of 47XXY (16%), 40 samples of trisomy 21 (80%) and 2 cases of trisomy 18 (4%) were identified.
Conclusion: Anomalies Trisomy 21 (41 ± 1.58) and 47XXY (9.62 ± 1.36) are significantly associated with mydatidiform mole disease (p <0.001). The highest percentage of samples with trisomy 21 and 47XXY had the disease. So, it indicates that these anomalies have the highest percentage in the disease.
Mina Zolfaghari, Behzad Khansarinejad, Ali Ganji, Zeinab Hamzehloo, Hamid Abtahi,
Volume 19, Issue 11 (2-2017)
Abstract
Abstract
Background: Ureaplasma and M. genitalium species belong to a kind of bacteria that are sexually transmitted and are the possible cause of pelvic inflammatory disease and nongonococcal urethritis, and et al. The aim of this study was to determine the urea plasma and Mycoplasma genitalium species frequency in women with vaginal infection and various sexual partners who referred to women, s health promotion and treatment center in Arak.
Materials and Methods: Endocervical swab samples from 110 women with vaginal infections referred to women’s health promotion and treatment center in Arak, were prepared. Patients’ personal information and identities during reception process were registered. The samples were transferred to the laboratory in the transport environment and after DNA extraction, were evaluated according to Real-time PCR assay.
Results: Urea plasma and Mycoplasma genitalium bacteria existed in 96(87.27%) and 4(3.63%) of patients, respectively. Among them, 4 cases had both bacteria infections. The amount of isolation in young women between 30-39 years old was more than others.
Conclusion: The results show that the colonization of urea plasma species in adult women is 40-80% and in studied group is 87.27%. These results indicate that with due attention to the increasing number of sexual partners and the increase of sexual activity, the urea plasma colonization of women will increase. In view of the potential influence of mycoplasma species on side effects resulted from pregnancy infection of mothers and mortality, on-time diagnosis and treatment will be increasingly essential.
Ahmad Hamta, Mahsa Mohammadi, Jamshid Ansari,
Volume 19, Issue 11 (2-2017)
Abstract
Background: Biological and epidemiological data indicate that the levels of vitamin D maybe affect the breast cancer risk. Vitamin D plays an important role in cell proliferation, apoptosis and tumor growth suppression. Vitamin D receptor is a critical mediator for the cellular reactions of vitamin D. Some of the epidemiological studies, reviewed the relationship between VDR gene polymorphism ApaI and breast cancer, but the controversial findings have been achieved.
Materials and Methods: In this study, a population-based case-control study including 140 patients and 160 healthy individuals of women in Markazi Province were evaluated using PCR-RFLP approach. Genomic DNA was extracted from blood samples using the salting-out procedure. Polymorphism of interest was determined by PCR-RFLP method using ApaI enzyme and statistical analysis was performed by SPSS software.
Results: Based on the results of this study, distribution of AA genotype in cancer and control groups was, 38.6 and 26.87, for AC genotype 55.00 and 66.87, and finally for CC genotype 6.43 and 6.26 respectively. The results of this study showed no association between ApaI polymorphism of the VDR gene and breast cancer(OR=0.903,CI=95%, 0.29-2.95.)
Conclusion: In this study, we found no association between ApaI polymorphism and breast cancer, which are consistent with the findings of some other researchs. It is necessary to examine a larger population to achieve more definitive results.
Niloofar Moradi, Mehdi Paryan, Behzad Khansarinejad, Mohammad Rafiei, Mahdieh Mondanizadeh,
Volume 19, Issue 12 (3-2017)
Abstract
Abstract
Background: Hepatocellular carcinoma (HCC) is the third major cause of cancer death worldwide. Hepatitis B virus (HBV) and HBx gene play an important role in the development of HCC by influencing signaling pathways. Since there is no detectable symptom in the early phase of HCC, there is need to find new HCC-specific markers with high sensitivity for early detection and diagnosis of HCC. On the other hand, by the advent and development of bioinformatic sciences, it is now possible to predict miRNAs as biomarkers, and their targets. Therefore, in the present study, based on the results of the bioinformatic software applications with different algorithm, we selected the miRNA targeting HBx and NOTCH1 mRNAs according to higher score, suitable connection with target gene and confirming them in more softwares.
Materials and Methods: First, the sequences of NOTCH1 and HBx genes were retrieved from NCBI. Afterwards, several software applications such as TargetScan, mirWalk, miRBase, Miranda, PicTar, miRVir, and DIANA were applied to predict miRNAs.
Results: Based on the high scoring by bioinformatics softwares and suitable targeting, miR-34a were selected to target NOTCH1 and miR-6510, miR-5193 and miR-214 were chosen to targetHBX gene.
Conclusion: Because of tumor suppression roles of miR-214 and miR-34a, they probably could be used as therapeutic strategy in cancer researches. It is also seems that the miR-5193 could act as a specific marker in Hepatocellular carcinoma.
Shadi Haghighat, Rasoul Zahmatkesh Roodsari, Reza Ranjbar,
Volume 20, Issue 4 (7-2017)
Abstract
Abstract
Background: Schizophrenia is a common mentaly disorder with high heritability and variable phenotype that they are placed in bipolar nervous disease family. Different genes are associated with this disease that among of them, DAOA gene plays a significant role. DAOA gene is located in the long arm of chromosome 13 and produces a protein called D-amino acid oxidase which is N - metyle D– aspartate (NMDA) receptor activator in the brain. The aim of this study was to investigate the association between DAOA gene polymorphisms and the risk of schizophrenia in Iranian population.
Materials and Methods: In this study, 100 patients with schizophrenia and 100 normal people as a control group were investigated. After genomic DNA extraction from blood samples, allele and genotype frequencies in patients and control group were determined by PCR-RFLP method. Data analysis was performed with Med Calc (ver 12) software.
Results: Frequency of GG, GA, AA genotypes in patients group was 18%, 42%, 40% and in normal group was 14%, 45%, and 41%, respectively. Statistical analysis revealed that there is a significant relationship between DAOA gene polymorphism with schizophrenia disease in the people under the age of 40.
Conclusion: According to the results of this study, the DAOA gene has significant association with schizophrenia in the Iranian population.
Nazli Khajehnasiri, Homayoun Khazali, Farzam Farzam Sheikhzadeh Hesari,
Volume 20, Issue 5 (8-2017)
Abstract
Abstract
Background: The advantageous effects of the regular moderate physical activity (Exercise) on reproduction is widely accepted, but until now, the alterations of the expression of hypothalamic arcuate nuclei gene affecting on reproduction axis in response to this type of physical activity are not clear. Therefore, the goal of the present investigation was to study the effect of one –month regular moderate physical activity on neurokinin B gene expression and reproductive axis hormonal changes.
Materials and Methods: In the experimental study, fourteen adult male Wistar rats were randomly divided into control and one- month regular moderate exercise groups. After one - month physical activity (20m/min), the arcuate nucleus was isolated from brain and stored in -80 refrigerators for neurokinin B gene expression assay by Real-time PCR method. In addition, serum samples were taken to assess the corticosterone, luteinizing hormone and testosterone levels by ELISA method. Data were analyzed by Independent t-test in SPSS.
Results: Neurokinin-B gene expression level was lower in the group which received physical activity than the control group(p<0.05). Also, corticosterone serum concentration was decreased in the physical activity group(p<0.05). By contrast, the physical activity induced luteinizing hormone and Testosterone serum levels evaluation in exersice group compared with control group (p<0.05).
Conclusion: Regular moderate physical activity may improve male reproductive performance by reducing the corticosterone hormone level and decreasing neurokinin B expression.
Olya Moshiri, Javad Sajedianfard, Mina Gheisari,
Volume 20, Issue 5 (8-2017)
Abstract
Abstract
Background: Pain is a protective process in the body. There are different pathways for pain control in the central nervous system. Descending pain control system is one of pathways. The periaqueductal gray (PAG) is a structure known for its role in pain transmission and modulation. The aim of this study is to investigate the percent of interaction between the left and right PAG in unilateral left foot induced pain.
Materials and Methods: In this study, 60 rats (280+30g) in six groups were used (3test groups and 3 controls groups). In test groups, 0.5 microliter lidocaine was injected in the left PAG, right PAG or both to make local anesthesia. In control groups, 0.5 microliter of normal saline were injected. After 15 minutes, 50 microliter of 2.5% of formalin were injected subcutaneously to right hind paw of rats and nociception was detected in every 15 seconds for one hour.
Results: The induction of unilateral pain (left hind paw) in rats, can affect not only the ipsilateral but also the contralateral PAG nucleus.
Conclusion: This study showed that the left and right PAG nuclei have significant role on unidirectional nociception in formalin test in rats. The contralateral PAG, however, has a minor effect on nociception.
Roghaieh Khakpay, Hanieh Feyzi, Farzam Sheikhzadeh Hesari,
Volume 20, Issue 7 (10-2017)
Abstract
Abstract
Background: 17β-Estradiol modulates nociception by binding to the estrogen receptors and also by allosteric interaction with other membrane-bound receptors like the NMDA receptors. The paragigantocellularis lateralis nucleus (LPGi) is also involved in the pain modulation. In this study, the role of NMDA receptors of the LPGi nucleus has been investigated in the 17β-estradiol-induced pain modulation in the ovariectomized rats.
Materials and Methods: In this study, the female Wistar rats in the range of 200-270 gr were used. In order to study the role of the NMDA receptors in the 17β-estradiol-induced pain modulation in the ovariectomized rats, primarily, rats were bilaterally ovariectomized and immediately cannulation of the LPGi nucleus was performed. Then, drugs were injected and 15 minutes later 50 μl of 5% formalin was injected into the rat's hind paw; and formalin-induced paw jerking behaviour was recorded for 60 min.
Results: The results of the present study showed that the intra-LPGi injection of 17β-estradiol significantly reduced the paw jerking behavior both in the first and in the second phases of formalin test. Pretreatment of the LPGi nucleus by NMDA receptor antagonist (AP5) neutralized the antinociceptive effect of 17β-estradiol on the paw jerking frequency in the both phases of formalin test; and induced hyperalgesia in the both phases of this behavior.
Conclusion: These results indicated that the intra-LPGi injection of 17β-estradiol produces modest analgesia on the formalin-induced inflammatory pain. Therefore, it can be concluded that the NMDA receptors of the paragigantocellularis lateralis nucleus are probably involved in the antinociceptive effect of 17β-estradiol in the ovariectomized rats.
Roghaieh Khakpay, Sanam Ansari, Fatemeh Khakpay,
Volume 20, Issue 8 (11-2017)
Abstract
Abstract
Background: Paragigantocellularis lateralis (LPGi) nucleus plays a key role in the processing of pain information related to the descending pain modulation. Also, 17β-estradiol is involved in the pain modulation. The aim of the present study was to investigate the role of estrogen receptors of LPGi nucleus in the 17β-estradiol-induced pain modulation in the ovariectomized rats.
Materials and Methods: In this study, the female Wistar rats (180-250 gr) were used. In order to study the role of the NMDA receptors in the 17β-estradiol-induced pain modulation in the ovariectomized rats, primarily, rats were bilaterally ovariectomized and immediately cannulation of LPGi nucleus was performed. First, drugs were injected and 15 minutes later 50 μl of 5% formalin was injected into the rat's hind paw; and then, formalin-induced paw jerking behavior was recorded for 60 min.
Results: Our results indicated that intra-LPGi injection of 17β-estradiol significantly attenuated paw jerking behavior in the both phases of formalin test. Intra-LPGi injection of estrogen receptor antagonist (ICI 182,780), had no effect on the paw jerking behavior. Pre-treatment of LPGi nucleus by ICI 182,780 counteracted the anti-nociceptive effect of 17β-estradiol both in the acute and in the chronic phases of formalin-induced paw jerking behaviour.
Conclusion: Based on the results of this study, it can be concluded that the intra-LPGi 17β-estradiol produces modest analgesia on the formalin-induced inflammatory pain in the ovariectomized rats, which is probably mediated via the esterogen receptors of this nucleus.
Mahsa Kazemi Roodsari, Farhad Mashayekhi,
Volume 20, Issue 9 (12-2017)
Abstract
Abstract
Background: Matrix metalloproteinases (MMPs) are vital for the degradation/remodeling of the extra-cellular matrix, and are involved in spiral artery formation and invasion of endometrium during implantation. Tissue inhibitor of metalloproteinase 1 (TIMP1), is expressed in the several tissues of organisms and inhibits MMP activity. The aim of this investigation was to study the association between single-nucleotide polymorphism (SNP) in the TIMP1 (rs4898) (372 T/C) with in vitro fertilization and embryo transfer (IVF-ET) outcome by AS-PCR.
Materials and Methods: A total number of 200 blood samples including 100 IVF negative and 100 IVF positive (control) were collected in this study. DNA was extracted for TIMP1 genotyping. The genotype and allele frequencies of 372T/C polymorphism were examined by Allele-Specific PCR.
Results: The genotype frequencies of CC, CT and TT in 372 T/C polymorphism of TIMP1 gene in IVF- samples were 1%, 98% and 1%, respectively, while for IVF+ group were 7%, 91% and 2%, respectively (p=0.07). The allele frequencies of C and T in the IVF- were 50%, 50%, respectively and in IVF+ were 47.5%, 52.5%, respectively. The genotype and allele frequencies of TIMP1 rs4898 (372 T/C) did not differ between the patients and the control group (p=0.07 and p=0.68, respectively).
Conclusion: The results of this study indicate that SNP 372T/C of TIMP1 may not be associated with IVF-ET outcome in this population. Further studies with larger numbers of patients and controls are needed to confirm our results.
Ahmad Hamta, Milad Pezeshki, Jamshid Ansari,
Volume 20, Issue 12 (3-2018)
Abstract
Abstract
Background: Biological and epidemiological data suggest that damage induced by endogenous and exogenous factors affects the integrity and stability of DNA and associated with susceptibility to breast cancer. The XRCC3 protein participates in DNA double-strand breaks and recombination repair. The aim of the present study was to evaluate associations between the risk of breast cancer and Thr241Met polymorphism in the XRCC3 gene.
Materials and Methods: In this study, the effects of Thr241Met polymorphism of the XRCC3 gene and the risk of breast cancer in a population-based case-control study inclusive 80 patients and 80 healthy individuals of women in Markazi province were evaluated. Genomic DNA was extracted from blood samples using the kit procedure. The genotypes of samples were determined by PCR-RFLP technique. Statistical analysis was done using SPSS software (estimation of χ2 and p-value) and the final results were determined.
Results: Statistically significant difference was observed between the two groups of patients and controls for three genotypes of the site rs861539 (p= 0.000). Genotype CT (p= 0.000, OR=2.352, CI= 95%; 2.431 - 39.948) and TT (p = 0.003, OR= 2.352, CI=95%; 0.611 - 9.049) significant associations were showed with risk of breast cancer. Instead, the genotype CC (p= 0.000) showed a protective role against susceptibility to breast cancer.
Conclusion: This study identified that there is significant association between Thr241Met polymorphisms of the XRCC3 and the risk of susceptibility to breast cancer, which is in accordance to some of researchers' studies.
Sima Akhavan, Rokhsare Memar, Leila Dehghani, Behnaz Ansari, Majid Ghasemi,
Volume 21, Issue 6 (12-2018)
Abstract
Background and Aim: Migraine is a common disease with throbbing and unilateral quality, which is accompanied by aura symptoms such as nausea, vomiting and photophobia. The level of procalcitonin is increased in migraine, which is due to the inflammation caused by a low level of serum serotonin. This study aims to investigate the level of serum procalcitonin in patient with migraine by prescribing serotonin agonist drugs.
Materials and Methods: In this case-control study, 40 migraine patients in the center of migraine patients of al-zahra university hospital in Isfahan were selected, among which 26 patients received serotonin as a treatment while the other 14 ones were treated by drugs other than sumatriptan agonist. Then, the level of procalcitonin in these patients was calculated and analyzed.
Findings: The results of the study showed that there was a difference between of the case and control groups in the level of procalcitonin in the serum of their sample blood tests. It seems that the case group patients have a lower level of procalcitonin compared to the control group.
Conclusion: Totally, the current study indicates the effect of serotonin in reducing the level of serum procalcitonin.
Mahdieh Mondanizadeh, Niloofar Moradi, Razieh Amini, Behzad Khansarinejad, Ghasem Mosayebi,
Volume 22, Issue 5 (11-2019)
Abstract
Background and Aim Chronic Lymphocytic Leukemia (CLL) is the most commonly occurring leukemia in adults, accounting for about 30-25% of total leukemia. One of the important etiological causes of this leukemia is the disruption of the Nuclear Factor Kappa B (NF-kB) signaling pathway. The two proteins of Apoptosis-Inducing Ligand (APRIL) and B-Cell Activating Factor (BAFF) play a role in the pathogenesis of this leukemia by affecting the NF-kB signaling pathway. In this study, due to the effect of miRNAs in regulating many cellular processes, the prediction of the prominent miRNAs targeting APRIL and BAFF transcripts in B-cell CLL patients was evaluated using specific and different bioinformatics programs.
Methods & Materials Afterwards retrieving the sequences of APRIL and BAFF proteins from the NCBI website, by using several programs including miRanda, TargetScan, miRWalk, DIANA and miRDB with different algorithms, the prediction of miRNAs targeting these genes was investigated.
Ethical Considerations This study was approved by the Research Ethics Committee of Arak University of Medical Sciences.
Results Based on the scoring system of bioinformatics programs, “hsa-miR-145-5p” and “hsa-miR-185-5p” were identified as miRNAs targeting APRIL gene, while “hsa-miR-424” and “hsa-miR-497”were miRNAs targeting BAFF gene. They were suggested for the practical studies in future.
Conclusion Based on the important role of APRIL and BAFF genes in the normal process of cell death and B-cell evolution, it seems that the mi-RNAs predicted by bioinformatics programs using different algorithms can be used as a diagnostic molecular biomarker to identify B-cell CLL patients.
Tahere Azimi, Malihe Bagheri, Mahdi Pariyan, Behzad Khansarinejad, Ashraf Zamani, Mahdieh Mondanizadeh,
Volume 23, Issue 3 (August & September 2020)
Abstract
Background and Aim: Cervical Cancer (CC) is the third most common malignancy in the women, the main cause of which is human papillomavirus (HPV). Both E6 and E7 oncogenes of the virus play an important role in its tumorigenesis. Today, methods available for screening CC are not capable of detecting the disease at an early stage. Therefore, it is important to identify new biomarkers for early detection of this cancer. For this purpose, in the present study, miRNAs targeting the two oncogenes E6 and E7 of human papillomavirus (types 16 and 18) were studied in CC by bioinformatics.
Methods & Materials: First, using the NCBI database, the E6 and E7 gene sequences were obtained for both human papillomavirus types 16 and 18. Then, using the miRBase and RNA22 bioinformatics databases, the most appropriate targeting miRNAs for these genes were selected.
Ethical Considerations: This study was approved by Ethics Committee of Arak University of Medical Sciences.
Results: Based on the P obtained from bioinformatics databases, miRNA including miR-92a-5p (P=7.51e-2), miR-195-3p (P=2.24e-1), miR-34a-5p (P=2.73e-1) and miR-155-5p (P=4.95e-2) were introduced for the two genes E6 and E7.
Conclusion: Results from bioinformatics studies revealed that of the four miRNAs identified, miR-155-5p and miR-92a-5p are probably the targeting miRNAs specific for the E6 and E7 genes, respectively. Therefore, it seems that these miRNAs can be a suitable candidate for in vitro studies in CC patients.
Javad Vakili, Vahid Sari Sarraf, Towhid Khanvari,
Volume 24, Issue 1 (April & May 2021)
Abstract
Background and Aim: Obesity is a clinical condition that affects the growth-related hormonal system, especially in adolescents. High-Intensity Intermittent Training (HIIT) might reduce such destructive effects.
Methods & Materials: In this quasi-experimental research, among adolescent boys [age: 13-18 years, height: 154±8 cm, Body Mass Index (BMI): 27.05±1.4 kg/m²], 30 volunteer subjects were selected. The study participants were divided into 3 groups of short-term and long-term periodic exercises as well as controls. The short-term exercise group performed 9 bouts of 30 seconds with 150-second breaks between sessions; the long-term exercise group performed 4 bouts of 150 seconds with 240-second breaks between sessions 3 times a week for 8 weeks. Body composition and blood samples were obtained 48 hours before and after conducting the training protocol to measure the desired parameters. The changes of each index were analyzed by repeated-measures Analysis of Variance (ANOVA) at the significant level of P<0.05.
Ethical Considerations: This study was approved by the Ethics Committee of Tabriz University (Code: IR.TABRIZU.REC.1398.021).
Results: After 8 weeks of HIIT, growth hormone increased and thigh, arm circumference, and fat percentages decreased (P<0.05). However, no changes were detected in the insulin-like growth factor I, BMI, and weight of the examined subjects (P>0.05).
Conclusion: Short- and long-term HIIT have the potential to improve the growth hormone status and body composition of overweight adolescents.
Ali Asghar Ghalenoei, Hossein Ansari, Abolfazl Payandeh, Azizollah Arbabi Sarjou, Gholamreza Soleimani, Ali Meshkinian,
Volume 24, Issue 3 (August & September 2021)
Abstract
Background and Aim: Genetic and environmental factors are known to be related to the development of childhood Eczema. We aimed to assess the ecological factors associated with the prevalence of Eczema among children using the ISAAC questionnaire.
Methods & Materials: A total of 1600 primary school girls and boys were selected and examined by multistage random sampling in this cross-sectional study. Data were collected and completed using the International ISAAC Questionnaire, including demographic questions and risk factors through interviews with parents, especially the student’s mother. The data were analyzed in SPSS v. 19 software using the Chi-square test and multiple logistic regression.
Ethical Considerations: This study was approved by the Ethics Committee of Zahedan University of Medical Sciences (Code: REC.1397.481).
Results: The prevalence of current Eczema was 10.4% (95%CI: 8.9-11.9). Co-morbidity with Asthma and Rhinitis was 82.8% and 74.5%, respectively. In multiple logistic regression models, history of allergies in family members (OR: 2.35, 95%CI: 1.53-3.59), father snoring (OR: 3.00, CI95%: 1.98-4.55), Keeping any animal in (OR: 1.6, 95%CI: 1.002-2.58), family size (OR: 0.85, 95%CI: 0.68-0.95), exposure to tobacco smoke at home (OR: 2.84, 95%CI: 1.18-6.81), showed a significant relationship with the occurrence of students’ Eczema.
Conclusion: Exposure to tobacco smoke at home, keeping any animal at home are effective factors in the prevalence of Eczema in children. By eliminating these factors, the prevalence of this disease can be reduced. However, the mothers have the most crucial role in screening and early diagnosis of Eczema in school children regarding determinants in this study. According to the results of this study, it is suggested that parents’ education, especially mothers, be emphasized by paying attention to these favorable factors.
