Showing 25 results for Khalili
Behnoush Sadat Khalili, Javad Hamedi, Setareh Haghighat,
Volume 21, Issue 7 (2-2019)
Abstract
Background and Aim: The widespread use of antibiotics has been led to increased emergence of antibiotic resistant bacteria and high mortality and morbidity rates due to infectious diseases. Pseudomonas aeruginosa is one of the most important causes of nosocomial infections, which shows high resistance to a wide range of antibiotics. So, finding new and effective antimicrobial compounds in order to overcome antibiotic resistant infectious diseases is so critical. Screening of native actinobacteria can be an effective strategy to find novel antimicrobial compounds. The aim of current study was isolation, screening and identification of rare actinobacteria to find the strains which produce antimicrobial compounds against P. aeruginosa.
Material and Methods: Thirty samples of water and sediments were collected from Persian Gulf and Oman Sea and used for isolation of actinobacterial strains. After isolation of actinobacteria, their metabolites were extracted and their anti-P. aeruginosa activities were investigated. Minimum inhibitory concentration (MIC) of the most efficient extract was determined using broth microdilution method. Finally, the most efficient strain was identified.
Ethical Considerations: In this study, all principles of biosafety and bioethics have been considered.
Findngs: Fifty actinobacteria were isolated from water and sediments. Five isolates had considerable antimicrobial activity. MIC value of the most efficient extract against P. aeruginosa was 100 µg/ml. Molecular analysis of 16SrRNA showed that the most effective fermentation broth extract belongs to Micromonospora and has 99.8% similarity to M. chalcea.
Conclusion: The current study revealed that the water of southern Iran and their sediments are promising sources of potent rare Actinobacteria in the production of antimicrobial compounds against P. aeruginosa. |
Mohammad Khalili Kelaki, Ruholah Karimzadeh , Neda Soleimani, Seyed Masoud Hosseini, Maghsud Arshadi,
Volume 22, Issue 3 (8-2019)
Abstract
Background and Aim: Background and Aim: Photodynamic therapy is a minimally invasive approach to cancer, which is used to combine non-toxic photosensitizer and visible light to produce reactive oxygen species and destroy tumors. This study aimed to investigate the cytotoxicity effect of Graphene Oxide (GO)as an organic matter with many oxygen groups on photodynamic on destroying cancer cells.
Materials and Methods: This study was performed on breast cancer cells (MCF-7) in vitro. The study groups included the first group of drug with different concentrations of graphene oxide (333.3, 285.7, 230.7, 166.6, 90.9,
47.6 µg/ml), the second group co-drug and laser light irradiation and the control group consisted of cells treated only with laser irradiation and the control group was treated with no treatment. Cells were exposed to visible laser irradiation (405 nm) at 0.1 W / cm2. Cell viability was determined by MTT assay.
Ethical Considerations: This study with research ethics code SBU/S.1397.46A has been approved by research ethics committee at Shahid Behesht University of Tehran, Iran.
Findings: The results of in vitro experiments showed that the dark toxicity of graphene oxide at concentrations of less than the 90.9 μg / ml concentration had no significant effect on cancer cells. Also, laser light alone don’t has toxic effect on cells. But graphene oxide-mediated dynamic light therapy has reduced the bioavailability of cancer cells on average by 21% over dark toxicity. Results are presented as mean of three independent replications, standard error, and p< 0.05 was considered significant.
Conclusion: In this study, graphene oxide is fully biodegradable at concentrations below a certain value, but with increasing concentration, the toxicity effect increases. With exposure to light and graphene oxide, viability decreses that it is more effective for in vivio studies.
Mohammad Reza Soleyman, Mostafa Khalili, Alireza Soleyman Meiguni, Maryam Baazm,
Volume 22, Issue 5 (11-2019)
Abstract
Background and Aim recombinant DNA technique is a powerful and appropriate method for the production of protein biopolymers with specificity in amino acid sequence and spatial chemistry. Elastin-Like Polypeptide (ELP) is a biocompatible, biodegradable and non-immunological biopolymer used in various biotechnology studies. The ELP tag is a cheap, fast and non-chromatographic technique for purifying target proteins. In this study, pET expression vector was designed for the combination of ELP gene sequences and target recombinant protein in order to produce recombinant fusion protein with the ELP tag.
Methods & Materials MOD gene was transformed to E. coli-BL21 (DE3) cells after designing and synthesis among the XbaI and XhoI restriction sites in the pET-32a (+) vector of the clone. Then, colonies were isolated based on plasmid size and examined by cutting using restriction enzymes. The final recombinant colonies was verified using polymerase chain reaction method and DNA sequencing.
Ethical Considerations The Research Ethics Committee of Arak University of Medical Sciences approved all ethical considerations ofworking on laboratory animals (Code: 92-146-11).
Results Replacing the MOD sequence in the pET-32a vector (+) eliminated the components expressing the fusion tags (Thioredoxin, Histidine, and S-tag), the identification site of protease enzyme (tobacco etch virus), and multiple cloning site. In addition, it added specific restriction enzyme identification sequences of ELP gene and target gene. As a result, in the optimized pET-MODvector, 466 nucleotides reduced in size and the secondary structure was improved.
Conclusion Considering the improvement of spatial structure and reduction of pET-MOD vector size, as well as the possibility of the fusion of recombinant protein with the ELP tag, it is possible to use this vector for ELPyation of the target protein.
Ramin Parvizrad, Sara Khalili Dermani, Azam Ahmadi,
Volume 23, Issue 3 (August & September 2020)
Abstract
Background and Aim: Staphylococcus aureus is common pathogens of nosocomial infections. Nasal swabs in hospital staff is main sources of hospital infections are considered. The aim of this study was to determine the frequency of nasopharyngeal carriers of methicillin-resistant Staphylococcus aureus and microbial contamination of health care workers' cell phones in Emergency department of Vali-e-Asr Hospital in Arak City.
Methods & Materials: In this descriptive study, nose swabs and cell phone levels were taken from 70 health care workers in the emergency ward of Vali-e-Asr Hospital. The Staphylococcus aureus clinical isolates were identified using standard microbiological methods (catalase, coagulase, mannitol fermentation and DNase). The susceptibility to oxacillin and cefoxitin was detected by the disk diffusion and the mecA genes in this clinically isolated strain of staphylococci was investigated by Polymerase Chain Reaction (PCR).
Ethical Considerations: This study was approved by the Research Ethics committee of Arak University of Medical Sciences. (Code : IR.ARAKMU.REC.1396.282).
Results: According the results, Staphylococcus aureus was isolated in 16 cases, which 5 cases were methicillin-sensitive S. aureus (MSSA), and 11 cases were Methicillin resistant S. aureus (MRSA). Also, 3 cell phones were infected with Staphylococcus aureus, which 1 case was MRSA and 2 cases were MSSA.
Conclusion: The results of this study showed that frequency of MSSA strains is significant in emergency personnel of the hospital. Thus, regarding to the risk of epidemics due to nosocomial infections, periodic testing for the identification and treatment of carriers among employees for controlling and preventing hospital infections seems necessary.
Fatemeh Khalili Alashti, Shahrbanoo Dehrouyeh, Naqmeh Tavakoli,
Volume 26, Issue 6 (February & March 2024)
Abstract
Background and Objectives: The present study was conducted with the aim of investigating the effectiveness of problem solving training on life orientation, positive and negative emotions, and learned helplessness in incompatible couples in Qaemshahr city.
Materials and Methods: The method of the present research was an experiment and a quasi-experimental research design with a control group. The statistical population included all couples who referred to counseling centers in Qaemshahr city in 2023. 30 couples were selected as a sample using available sampling method and randomly assigned to two experimental and control groups (15 people in each group). Then, the subjects of the experimental group received problem solving training during 8 sessions of 60 minutes. In order to collect data from life orientation questionnaires (Shier and Carver, 1994), positive and negative affect (Watson et al., 1988) and learned helplessness (Quinless and Nilsson, 1988) in two stages before and after training in both groups (experiment and control) were used.
Results: The findings from the analysis of covariance test showed that problem solving training improved optimism in life orientation, increased positive emotion, and reduced learned helplessness in the tested group (p=0.05).
Conclusion: According to the findings of the present research, in the field of therapy and counseling of incompatible couples, training in problem solving for the compatibility of couples and solving their conflicts; It looks very efficient and promising.
