Showing 144 results for Arya
Maryam Delfan Biranvand, Mahmud Mahmudi, Maryam Rstin, Ali Sheykhian,
Volume 12, Issue 1 (5-2009)
Abstract
Background: There are citrullin peptides in Rheumatoid Arthritis (RA) patients that are changed by Peptidyl Arginine Deiminase enzymes (encoded by PADI genes). An association between RA and PADI-4 haplotypes has been reported by researchers. The aim of this study is evaluation of PADI-4 Gene polymorphism in patient with Rheumatoid Arthritis referring. Methods and Materials: In this cross-sectional study we extracted the genomic DNAs from the whole blood samples of 50 patients with RA ( on the basis of ACR criteria) referring to Emam Reza hospital in Mashhad and a control group involving 50 healthy khorrasanian participants. DNA genom was extracted with nonenzymatic salting out method. Genotypes were determined by PCR simple sequence specific primers (PCR-SSP). Data were analyzed with Chi-Square and Fisherexact Test. Results: 58% of the patients had genotypes of 1and 2 38%: 1,4, 2%: 1b,2 ,and 2%: 2,4 .In the control group, 72% had genotype 1,2 22%: 1 and 4, 6%: 1b,2 while none of them had genotype 2,4. Conclusion: There was not any significant relationship between the presence of different PADI-4 genotypes and RA in Khorrasanian population
Akram Bayati, Abolfazl Mohammad Beigi, Babak Eshrati, Maryam Jafari,
Volume 12, Issue 2 (9-2009)
Abstract
Background: Providing health not only is responsibility of governmental organizations but also is responsibility of Community Health Vounteers help to health staff in health centers and try to promote people's knowledge and practice about their health. This study is conducted to determine knowledge and practice of the volunteers before and immediately after education and evaluated long – term education effects. Methods and Materials: This interventional (semi- experimental) study was carried out on 78 health communicators Arak with census method. In Arak axis education were included check of vital signs and rescue method during disasters. Methods of education were lecture, smallgroup, problem solving, and practical training in Skin lab. Data were gathered with questionnaire and check list and were analyzed with Paired T-test and Pearson correletion coefficient. Data was analyzed using paired t-test and Pearson correlation coefficient. Results: There was a significant difference between mean of knowledge and practice scores before and immediately after training (p<0.001). But the relationship between scores immediately after training and one year later, was not significant. Conclusion: It seems updated knowledge and practice of health volunteers change health behavior, promote knowledge and practice and decrease health care expenses.
Maryam Tajabady Ebrahimi, Mohamad Amin Hejazy, Reza Ghafary, Parvaneh Jafari,
Volume 12, Issue 2 (9-2009)
Abstract
Background: In order to selected indigenous potential probiotic bacteria, we surveyed antagonistic activities of 22 strains of acid and bile tolerant Lactobacillus, isolated from traditional dairy products by biochemical and molecular methods. Methods and Materials: In a fundamental practical study assessment of antimicrobial activity of this strain with neutrallized and Dual layer two methods against bacterial pathogene such as E-coli, L.monocytogenes, S.auteus and Y.entercolitica was done. These strain were identified with two methods for determining of biochemical and sequence of 16Sr DNA. Results: Dual layer method based on the growth of zone diameter were estabilished in three groups of strains inhibitors, semi inhibitors and non inhibitors. Neutralize method around well acidic extract containing strains C5i4, Y144, K213, C4i2, C612 and neutral extract C5i4 zone blight strains was observed. Based on the results, sequence area 16Sr DNA of four strains inclulde C4i2, C1d2, Y2c4, D3b1 indicator bacteria that revealed the highest percentage of inhibitor effect of bacterial indicators, were duplicate and sequency. So four strains L.Bacilus Pentosus, L.Bacillus Bervis and L. Bacillus Paraplantarum, were indentifed respectivey. Conclusion: It seems that indigenous lactobacillus from Iranian dairy products have potential as probiotics. So use of them as bio preservative prevent food bacterial contamination.
Shahram Baraz, Maryam Rostami, Fereshteh Farzianpor, Abdolrahman Rasekh,
Volume 12, Issue 2 (9-2009)
Abstract
Background: The most important determinant factor in elder’s quality of life is healthy. Since health can be declined by aging, in health promotion consider an effective model of care can be vey important. We surveyed the effect of Orem Self Care Model on quality of life (QOL) of elderly in Masjed Solaiman. Methods and Materials: This is a pre and post quasi-experimental study was carried out on 349 elderly clients which were selected from 11 health care centers in Masjed Solaiman in 2007-2008. Orem Self Care program was performed during a 3 months in days that the clients came to health care centers. Data were gathered by a short form questionnaire (SF36) before and after the intervention and was analyzed by descriptive statistics and paired t-test. Results: Results showed a significant increase in mean scores of different dominations of QOL including health, physical performance, physical role- performance, emotional role- performance, social performance, body pain, power and energy, general health perception, and overall mean of QOL. Paired t-test showed a significant difference (p<0.001) between all of dominations of the quality of life. Conclusion: Performing Orem Self Care educational program had a positive influence on improving QOL of elderly.
Maryam Tehranipour, Javad Bahar Ara, Maryam Mostafaee,
Volume 12, Issue 3 (10-2009)
Abstract
Abstract Background: Fetal Cerebrospinal fluid (CSF) develops viability and proliferation of nerve cells. Also this fluid contains many valuable factors for protection of nervous system injury cells. In this research, the effect of cerebrospinal fluid intraperitoneal injection on alpha motor degeneration after sciatic nerve compression in rat was determined. Materials and Methods: In this experimental-laboratory study, 18 male Wistar rats divided randomly in 3 groups (control, compression, and experimental). In compression and experimental group, right sciatic nerves were highly compressed. CSF was injected in experimental group each three days. After 1 month care, all rats were cordially perused by 10% formaldehyde and their L4-L6 lumbar segments of spinal cord were sampled and with processed for histological examination, the paraffin blocks were serially cut (7mm). Slices were stained with toluidine blue and numerical densities of motoneurons in spinal ventral horn were estimated stereological (dissector) technique. Quantitative data were analyzed by T-test. Results: Significant reduce in motoneurons number of compression group (47026) in comparison with control group (173978) was seen. Also there was significant difference between compression and experimental groups (992±141) in neuron density. Conclusion: CSF intraperitoneal injection may have a beneficial effect in neural regeneration.
Maryam Tehranipour, Jina Khayyatzade, Reyhane Javaheri Fard,
Volume 13, Issue 1 (4-2010)
Abstract
Background: Following the reduction of neurons due to peripheral nervous injuries, the number of neuroglial cells also decline because of not receiving vital factors. The aim of this study was to determine the protective effects of curcuma longa total extract on spinal cord neuroglia cell degeneration after sciatic nerve compression in rats. Materials and Methods: In this experimental clinical- trial, Wistar rats were categorized in five groups (control, compression, treatment1, treatment 2 and treatment 3), each including six rats. For inducing the injury in the compression and treatment groups, the right sciatic nerve in the upper thigh was compressed using clamp forceps. In the treatment groups, 100mg/kg doses of the extract were injected in group1(3 times a day), group 2 (6 times a day) and in group3 ( 9 times a day). After 28 days, following being anesthetized, the rats underwent perfusion and samples were taken out of the lumbar segments of their spinal cord. Then the samples, after going through tissue processes, were cut in 7 m serial sections and stained in blue toluidine. Through the stereological quantitative technique, neuroglial cells were counted. Results: A significant decrease was seen in the number of neuroglial cells in the compression group (6913±208) in comparison with the control group (10184±791). Also, through the comparison of the compression group with treatment group 1(7077±293), treatment group2 (9372±252) and treatment group 3 (8715±252) a significant difference among dnsity of neuroglial cells in groups and 3 with conppnessin group was seen. a remarkable increase in the numerical density of neuroglial cells was obtained (p<0.05). Conclusion: Due to its antioxidant effects, curcuma longa extract increased the numerical density of neuroglia cells following the compression of the sciatic nerve. The antioxidant effects of this extract probably inactivate the apoptosis channels which have been activated due to peripheral nerve injuries.
Morteza Behnam Rassouli, Nargess Ghayour, Majid Afsharian, Maryam Tehranipour, Mohammad Bagher Ghayour,
Volume 13, Issue 1 (4-2010)
Abstract
Background: Oxidative stress is one of the probable molecular mechanisms involved in lead (Pb) neurotoxicity. On the other hand, lemon balm (Melissa officinalis) which is widely used in traditional medicine, has a high antioxidant activity. In this study, the protective impacts of Melissa officinalis on the adverse effects of Pb toxicity on learning ability were investigated. Materials and Methods: In this experimental-clinical trial, 40 virgin Wistar rats were mated and divided into control, control positive (vitamin C+Pb), control negative (Pb), and 3 Pb and Mellisa experimental groups. Mellisa was orally administered in three doses including + 25, 50 and 100 mg/ kg of body weight daily. Treatment started from 7th day of gestation and continued through pregnancy and lactation periods. The three month- old offsprings in each group were assessed in terms of memory and learning ability by Morris water maze test and the results were compared between the groups. Results: Exposure to Pb during and after gestation leads to learning disorders. While concomitant administration of Pb and Melissa, as well as vitamin C can, to a large degree, reduce the adverse effects of Pb on learning abilities. Conclusion: Since no significant differences were obtained from the comparison of results in the control, Pb+M and vitamin C+Pb groups, it can be concluded that Melissa has antioxidant impacts equal to vitamin C. Therefore, similar to vitamin C, Mellisa can decrease the neurotoxic effects of Pb.
Gholamreza Sharifirad, Parastoo Golshiri, Hossein Shahnazi, Maryam Barati, Akbar Hassanzadeh,
Volume 13, Issue 1 (4-2010)
Abstract
Background: Nowadays, mothers have ceased to breastfeed due to several factors and resort to bottle-feeding or other forms of complementary feeding for their children. This results in physical and emotional problems for children, and socio-economic harms in communities. The aim of this study is to assess the impact of BASNEF model based education on successful breastfeeding in pregnant mothers referring to health centers in Arak. Materials and Methods: This quasi-experimental study was carried out with the participation of 88 pregnant women. The participants were randomly allocated to interuention and control groups. Instructions were given in four sessions on the basis of BASNEF model and behavioral objectives. Eventually, the collected data were analyzed by using statistical tests. Results: Mean scores of knowledge, attitude, enabling factors and subjective norms in the inerurntion group before and after education showed a significant difference in comparison with control group (p<0.001). Conclusion: Model-based education has positive impacts on behavior improvements. Thus, in health programs instead of applying traditional instructions, appying planned education programs based on health education models that have measurable and reliable effects is suggested.
Morteza Kafaee Razavi, Saeedeh Ebrahimpour, Maryam Tehranipour, Morteza Behnam Rasouli,
Volume 13, Issue 2 (6-2010)
Abstract
Background: From a physiologic point of view, memory is formed through changes in synaptic conductivity from one neuron to the next. These changes result in the formation of long-term potentiation for conducting a message. Cannabis sativa has more than 61 components that are called cannabinoid. The aim of this study was to investigate the effect of aquatic extraction of cannabis sativa seed on spatial memory consolidation in rats. Materials and Methods: First, 40 Wistar rats, each nearly 250-320g, were divided into four experimental groups and a control group. Cannabis sativa seed was extracted with Soxhlet apparatus. To consolidate spatial memory, Morris water maze (MWM) test was administered in seven sessions, four trials for each session. Experimental groups 1, 2, 3 and 4 received 50mg/kg-1, 100mg/kg-1, 150mg/kg-1, 210mg/kg-1 peritoneal injections (IP), respectively. After memory consolidation, the position of platform area was changed and MWM was repeated for five days. Results: The results show that experimental groups 1, 2, and 3 had a significant decrease in learning time in the comparison to the control group (p<0.05), whereas experimental group 4 with a 210mg/kg-1 dose did not reveal any significant difference in comparison to the control group (p<0.05). Conclusion: It is likely that this long-term potentiation is done through depolarization-induced suppression inhibition (DSI) and depolarization-induced suppression excitatory (DSE) mechanisms in the CA1 area of Hippocamp that lead to neuro-plasticity through neurotransmitter regulation.
Nahid Salarkia, Maryam Amini, Maryam Eslami, Monireh Dadkhah, Telma Zoghi, Homa Haidari, Morteza Abdollahi,
Volume 13, Issue 2 (6-2010)
Abstract
Background: Inappropriate feeding is one of the major causes of malnutrition in children. This study was carried out to get an insight into mothers’ views and beliefs about the role of complementary feeding in children under the age of two. Materials and Methods: In this qualitative study, data were collected through 11 focus-group discussions (FGDs) with mothers, who had children under the age of two, in urban and rural areas of Damavand in 2007. Each FGD was held in the presence of a moderator, two note takers, and an observer. At the end of field work, all notes were collected and coded according to the objectives of the study. Then the subgroups for each objective were obtained, and emerging themes were extracted and reported. Results: In the study, six general themes were identified:(1) The mothers viewed complementary feeding as simple, soft, light foods which are especially made for children (2) the best time for introducing complementary feeding was 6 months of age (3) there were no complementary local foods in Damavand (4) for children under the age of one, a different type of food was separately prepared (5) home-made complementary food was preferred over the ready made type, and (6) children were fed with table food from the first year of life. Conclusion: Despite their sufficient knowledge about the advantages of complementary feeding, the majority of mothers, due to some socio-cultural limitations, had a weak performance. In this regard, in addition to making revisions in current training programs and protocols, providing effective and goal-oriented educational programs for mothers and others who take care of children is strongly recommended.
Maryam Tehrani Pour, Homa Mahmoodzade, Tooba Ghadamyari,
Volume 13, Issue 3 (9-2010)
Abstract
Background: Salvia staminea, belonging to lamiaceae family, has positive effects on the nervous system and possesses anti-oxidant and anti-inflammatory effects. Hence, this study was conducted to determine the neuro-protective effects of salvia and its total ethanol extract on spinal cord motoneurons in Wistar rats. Materials and Methods:In an experimental trial, 54 male Wistar rats were divided into nine groups of six: Control, compression, treatment A (ethanol extract of root in 25, 50, and 75 mg/kg doses), treatment B (aqua extract of root in 25 and 50 mg/kg doses), treatment C (aqua extract of leaves in 50 mg/kg doses), and treatment D (ethanol extract of leaves in 75 mg/kg doses). In compression and treatment groups, 28 days after inducing impairment in α motoneurons, sampling of the left leg sciatic nerves was done in the rats. Following tissue passage, 7 micron cuts were obtained and painted with blue toluidine. Neuralgia motoneurons count was, then, carried out through steriology and dissector methods. Results: Neuron number density in the rats treated with 50 mg/kg doses of total aqua and ethanol extracts of leaves and 75 mg/kg dose of ethanol extract of root showed significant differences in comparison to that of the compression group (p<0.05). Conclusion: The results of this study prove the neuroprotective effects of these extracts on neuralgia motoneurons of spinal cord.
Afsaneh Nourozi, Abolfazl Jafari, Reza Badei, Maryam Gadimi,
Volume 13, Issue 3 (9-2010)
Abstract
Background: Pain following tonsillectomy is common in children, and its elimination due to its subsequent complications is essential. Therefore, this study was conducted with the aim of obtaining the best method of administering ketamine for reducing pain following tonsillectomy with the greatest efficiency and the least side effects. Materials and Methods:This study was a double-blind clinical trial which was conducted on 92 children, aged 3-9, who had been admitted for tonsillectomy. The patients were randomly divided into oral ketamine and ketamine injection groups (each containing 46 patients). Thirty minutes before the operation, all the patients were given apple-juice ketamine injection group received normal apple juice without ketamine, whereas the oral ketamine group received 5mg/kg ketamine with the apple juice. Three minutes before tonsillectomy, 0.5 mg/kg ketamine was injected to the peritonsillar area in ketamine injection group while the same volume of saline was injected to the oral ketamine group. Data were registered, gathered, and, then, analyzed using Wilcoxon, Man Whitney, and t tests. Results: Demographic data and blood pressure before and after the operation did not have significant differences in the 2 groups. In terms of the duration of operation time, a significant difference was observed between the two groups the operation time in the ketamine injection group was shorter (p=0.006). Also, pain score in the ketamine injection group was less than the oral ketamine group. Conclusion: Ketamine is effective in reducing the pain after tonsillectomy operation. Peritonsillar injection of ketamine is more effective than its oral usage.
Parsa Yousefi Chaijan, Fatemeh Dorre, Zahra Moghaddasi, Maryam Mashayekhi,
Volume 13, Issue 3 (9-2010)
Abstract
Background : Urine tract infection(UTI) among children is the most prevalent bacterial infection and the second prevalent infection after Viral Flu.Regarding this cfact that urine culturing has very important role in diagnisis and perseverance of this disease and sampling method has significant effect on this disease remedy.
In this study the effect of genital area ablution on decreasing the contamination of urine culture is examined.
Methods and Materials: This study is in the form of case_controlled.In this study 620 little girls (3-12 years –old) referring to Amir Kabir children special clinic are examined.Population under investigation was cpatients who had not history of taking antibiotic during last seven days, sondage and genital anomaly and inflammation and genital discharge.
Patients were randomly entered in two different groups one with ablution and the other without ablution.After data collection, available inputs were statistically analyzed with SPSS and the effect of genital area ablution was determined on decreasing the contamination of urine culture.
Results: Among 310 patients in with_ablution group 11 patients were reported UTI(3/7%).3 patients were contaminated (1%),296 patients were normal (95/3%).Among 310 patients in the second group,14 patients were reported UTI(4/7%),6 patients were contaminated(2%),290 patients were normal(93/3%).Contamination rate in with_ablution group were 1% and in the other group was 2%Based on Pvalue :0/491 in both groups no significant statistically difference were found between these two groups and the analysis result distribution in both groups in a=0/05 level were statistically the same.
Conclusion: In both group no significant statistically difference were found between these two groups ,so we concluded that the genital area ablution doesn’t have such an effect on decreasing the contamination of urine culture and we don’t suggest ablution of genital area in children before providing urine sampling.
Behrokh Farahmand, Mahvash Khodabandeh, Fereidoun Mahboudi, Fatemeh Fotouhi, Farzaneh Barkhordari, Maryam Saleh, Masoumeh Tavasoti Kheiri,
Volume 13, Issue 4 (1-2011)
Abstract
Background: Influenza is a contagious respiratory infectious disease out breaking in cold seasons of the year. The outbreak of the new influenza A (H1N1) virus in 2009 involved large populations of the world with considerable mortality. Hemagglutinin (HA) molecule, the main surface glycoprotein of the influenza virus, is one of the key factors for serological diagnostic kits and vaccine development. Thus establishment of HA gene bank of the circulating influenza viruses is essential in gaining quick access to large amounts of protein. Materials and Methods: The first step in providing such a bank is detection and isolation of HA full genome and its subunits by using specific primers and cloning them in proper vectors. For this purpose, using standard virus genome (A/New Caledonia/20/99(H1N1)) cultured on MDCK cell, HA coding gene was proliferated by RT-PCR using specific primers. Results: Isolation and cloning of the HA gene was verified by RT-PCR, enzyme digestion and determining nucleotide synonymy. Through the use of specific cloning primers, different HA gene constructs were propagated for expression of the gene in insect cells and E.coli bacteria. Conclusion: The results indicated the complete compatibility of the extracted HA gene with the influenza (A/New Caledonia/20/99(H1N1)) hemagglutinin. It makes it possible to use the gene as a source of cloning in a variety of eukaryotic and prokaryotic expression systems
Mohammad Hasan `ghosian Moghaddam, Maryam Maleki,
Volume 13, Issue 5 (Supplement of Quran and Medicine 2011)
Abstract
Background: Eating and drinking according to health instructions is a major factor in health. Islam recommendations regarding nutrition ensure not only physical health but also mental health. While advising Muslims to consume meat as much as their body requires, Islam forbids certain types of meat. This study is to investigate the effect of pork consumption on human health from the perspective of Quran and medicine. Materials and Methods: Relevant Quranic verses and Hadith and the existing literature were reviewed and described through the use of specific keywords. We utilized relevant materials from the Holy Quran and Hadith and also conducted a literature search through specific related keywords. Results: In four Surahs of Quran and Hadith from the infallible Imams, pork consumption is forbidden. The presence of a group of amines, carcinogenic agents, high fat, and a high level of growth hormones turns pork into a sutoxin product. In addition, the presence of significant zoonotic diseases, quick decay, and easy contamination are other reasons which make pork consumption harmful for human. Conclusion: Noticing the significant role of nutrition in physical and mental health, divine religions have pronounced recommendations on using decent and healthy nutrition and refraining from using the unhealthy materials. Pork is one of these unhealthy and unusable materials which unfortunately, due to lack of sufficient awareness, is still consumed by some.
Maryam Davoudi, Alireza Kamali,
Volume 14, Issue 1 (3-2011)
Abstract
Background: The main advantage of regional anesthesia is the elimination of post-operative pain without the need for narcotics, which leads to a more rapid outpatient recovery and faster return to consciousness in pediatric patients. The aim of this study was to compare caudal anesthesia with midazolam and neostigmin co-administered with bupivacain in reduction of post-operative pain in lower abdominal surgery in pediatrics (2- 8 years old). Materials and Methods: In a double-blind clinical trial, 45 pediatric patients were randomly allocated to midazolam, neostigmine, and placebo groups. Induction of anesthesia was done with inhalation of halothane and O2. Bupivacaine 0.25% in 0.5 ml/kg with midazolam in 50μ/kg was administered in caudal form to the first group, and bupivacaine 0.25% in 0.5 ml/kg with neostigmine in 2 μg/kg dose was administered to the second group. The third group received the placebo plus bupivacaine 0.25% in 0.5 ml/kg. Results: Mean durations of analgesia in neostigmin, midazolam, and placebo groups were 16.5± 0.25, 12.3±0.22, and 6±0.11 hours, respectively (P<0.001). Also, in the neostigmin group, mean score of pain in outpatient recovery 6 , 12 , 24 hours post operatively was less than that in the other two groups (P<0.001). Conclusion: Duration of analgesia period in neostigmin group was more than that in midazolam and placebo groups
Akram Eidi, Mahsa Al-Ebrahim, Maryam Eidi, Ali Haeri Rohani, Pejman Mortazavi,
Volume 14, Issue 1 (3-2011)
Abstract
Background: Molybdenum is an essential trace element for both animals and plants. Molybdenum (Mo), which functions as a cofactor for a limited number of enzymes including xanthine dehyrogenase, aldehyde oxidase, and sulfite oxidase in mammals, is believed to be an essential trace element in animal nutrition. The aim of this study is to evaluate the hepatoprotective potential of sodium molybdate against carbon tetrachloride (CCl4) induced liver damage. Materials and Methods: In an experimental study, adult male rats received daily oral administrations of different doses of sodium molybdate (0.05, 0.1, and 0.2 g/kg bw) along with intrapertioneal CCl4 (50% CCl4 in olive oil, 1 ml/kg bw) twice a week for 28 consecutive days. Results: Histopathological examinations in CCl4-treated rats showed extensive liver injuries characterized by extensive hepatocellular degeneration and necrosis, fat degeneration, and inflammatory cell infiltration while histopathological changes induced by CCl4 were significantly attenuated by sodium molybdate treatment. Conclusion: The results of this study suggest that sodium molybdate could protect liver against the CCl4-induced oxidative damage in rats, and this hepatoprotective effect might be contributed to the protection of liver by preventing the toxic chemical reactions which generate oxidative stress, lipid peroxidation, and molecular changes which ultimately lead to liver tissue necrosis.
Maryam Yadegari, Mozafar Khazaei, Yazdan Hamzavi, Ali Reza Toloei,
Volume 14, Issue 2 (5-2011)
Abstract
Background: Falcaria vulgaris has different properties and it used as dietary and medicinal herb in the west of Iran. Previously, we showed that this plant has protective and repairing effect on gastric ulcer were demonstrated .The aim of present work was to investigate the effects of F. vulgaris extract on female rat's fertility.
Material and methods: In this Exprimental study Virgin female NMRI rats (160-190 gr) were used in three experiments. In each experiment, animal divided into two subgroups (n=8): control which received Distilled Water (DW) (2cc/kg) and case which received herb extract (150mg/kg) interaperitonealy. In first experiment, animals received single dose of extract or DW.
In second experiment، female rat received extract or DW in perimplantation period (day 1 to 5 of pregnancy) and in third experiment ( day 5 to 7 of pergnancy). In both experiments implantation sites and neonates were counted in three experiments. Data were analyzed by T-Test test and p<0.05 were considered significant.
Results: All three experiments showed significant differences between control and case groups in implantation sites and neonates number. This differences were more prominence in first and third experiment.
Akram Eidi , Sara Moghadam-Kia , Jalal Zarringhalam Moghadam, Shamsali Rezazadeh, Maryam Eidi,
Volume 14, Issue 4 (9-2011)
Abstract
Background: In traditional medicine, Olive oil (Olea europaea L.) from Oleaceae family is known as a remedy for alleviating pain. This study investigates the antinociceptive effects of olive oil on male adult NMRI mice.
Materials and Methods: In this experimental study, using the acetic acid-induced writhing and formalin tests, the anticipative effects of olive oil were evaluated. Olive oil (1, 5, and 10 ml/kg bodyweight), morphine (10 mg/kg bodyweight), and indomethacin (10 mg/kg bodyweight), as standard drugs, were injected intraperitoneally. The control group did not receive any treatment. Data were analyzed using one-way ANOVA and Tukey test.
Results: Olive oil significantly decreased acetic acid-induced abdominal writhes (P<0.001). Olive oil could only decrease the induced pain in the second phase of the formalin test (P<0.001).
Conclusion: Olive oil decreases inflammatory pain (the second phase of the formalin test and acetic acid-induced writhing tests), but it has no significant effects on neurogenic pain (the first phase of the formalin test). Further studies are required to elucidate the antinociceptive effects of olive oil.
Farima Zahedi, Mitra Heidari Nasr Abadi, Maryam Taj Abadi Ebrahimi, Mohammad Shabani, Helia Aboutalebi,
Volume 14, Issue 4 (9-2011)
Abstract
Background: Today, there is growing interest in using traditional medicine for healing cutaneous wounds. Probiotics are defined as different microorganisms that may have positive effects on prevention or treatment of special pathologic conditions. The present study was designed to investigate the effect of Lactobacillus brevis on cutaneous wound healing.
Material and Methods: In this experimental study, through phenol-sulfuric acid method, 22 strains of lactobacillus isolated from dairy-traditional products were investigated in terms of exopolysaccharide production. Lactobacillus brevis, which had high exopolysaccharide (EPS) production, was selected. A wound was created on the back of male Wistar rats in control, negative control, and experimental groups. Control and experimental groups underwent regional treatment by eucerin and eucerin contained Lactobacillus brevis, respectively, but the negative-control group did not receive any treatment. On days 1, 7 and 21, the rats were killed and their cutaneous wound samples were studied. Data analysis was done through SPSS version 11.5.
Results: The percentage of wound healing (99.53%) and inflammation in the experimental group on day 21 compared to control (90.55%) and negative groups (91.14%) was significantly higher (P<0.001). The number of neutrophils in the experimental group decreased in later phases of wound healing compared to the control and negative control groups.
Conclusion: The present study showed that Lactobacillus brevis significantly decreases inflammation and accelerates wound healing in treated rats. The findings of this study can be applied clinically in near future
