Showing 33 results for Asad
Rahman Soori, Mohammad Reza Asad, Mahboobe Khosravi, Sadegh Abbasian,
Volume 19, Issue 4 (7-2016)
Abstract
Background: Irisin is a new myokine that exerts its positive effects via increasing energy expenditure. The purpose of this study was to determine the effect of submaximal aerobic training on serum irisin level and irisin-related insulin-resistance change in obese men.
Materials and Methods: In this study, 26 obese men were selected as statistical sample and they were divided into experimental groups. Former group (13 men) consisted of subjects who carried out exercise training with 50-60 percent of maximum max heart rate for 10 continuous weeks (3 sessions per week for 30 to 45 minutes in each session). Later group was consisted of subjects who do not carried out exercise training. After the interventions of study, irisin serum levels, insulin resistance indices, body composition and maximum oxygen consumption were determined. Finally, data were analyzed by Stata software.
Results: Statistical data analysis illustrated that the amounts of irisin serum were significantly increased in exercise training group than control group (14.75±6.22; p=0.022) after the intervention. Also, only body fat percentage (1.242±0.57) and max oxygen consumption (1.509±0.328) of subjects were significantly increased (p=0.034 and p=0.0001, respectively). However, there were not statistically significant changes in irisin- related insulin-resistance indices (p>0.05).
Conclusion: Current study presented that serum irisin was increased after 30 submaximal exercise training sessions regardless of insulin resistance changes in obese men.
Mohsen Golnari Maranni, Mohammad Rabbani Khourasgani, Mohammad Ali Asadollahi, Rasoul Shafiei,
Volume 19, Issue 11 (2-2017)
Abstract
Abstract
Background: Prevalence extension of antibiotic resistant bacteria has raised concerns about control of infections especially nosocomial infections. Many attempts have been done to replace antibiotics or limit their use. The use of antimicrobial agents produced by bacteria as antibiotic replacement has been promising in recent years. The goal of this study was to isolate Bacillus strains and evaluate their antimicrobial activity against some standard pathogens and clinical antibiotic resistant strains.
Materials and Methods: In the present study, Bacillus strains were isolated from various resources and identified by 16S rDNA PCR method. Then, the phylogenetic tree of the isolates was constructed and antimicrobial activity of the isolates was investigated against some standard pathogens and clinical antibiotic resistant strains using spotting and well diffusion methods.
Results: Eight Bacillus strains were isolated from 15 different samples. Based on the molecular identification, the isolates were identified as B.pumilus, B.coagulans, B.licheniformis, B.endophitycus and B.amiloliquefaciens. The results showed that isolates have antimicrobial activity against meticilin-resistant Staphylococcus aureus, vancomycin resistant enterococci, Klebsiella, Acinetobacter, Salmonella, Shigella, Listeria, Streptococcus and Escherichia coli.
Conclusion: In this study, isolated Bacillus strains produced antimicrobial agents against pathogens and antibiotic resistant strains and inhibited their growth.
Rokhsareh Meamar, Maryam Ostadsharif, Ahmad Chitsaz, Mojgan Asadian Ghahfarokhi, Mehdi Kazemi, Seyed Morteza Javadirad,
Volume 20, Issue 4 (7-2017)
Abstract
Abstract
Background: Vitamin D was recognized with protective effects on nerve cells of Parkinson’s patients. The relationship between several VDR gene polymorphisms and age and risk of the disease was determined. Also, the relationship between VDR gene FOKI genotypes and PD was specified. The main goal of this study is to evaluate the relationship between polymorphic loci of FokI, TaqI, BsmI, ApaI and serum factor related to vitamin D metabolism in Isfahan population.
Materials and Methods: Case- control study of 125 Parkinson’s patients with their matched control individuals has been investigated based on Parkinson's disease brain bank criteria of Great Britain. After receiving consent, serum levels were measured. The genetic material was isolated by Miller protocol and polymorphisms has been analyzed and confirmed by repeated PCR-RFLP.
Results: Comparing the five serum factors between healthy subjects and patients with Parkinson's disease, we have shown a significant reduction in the levels of calcium, ALP and PTH (p<0.01). However, none of the levels of vitamin D and phosphate show any kind of significant relationship between patients and control subjects. Concentration of blood serumic factors including calcium and PTH showed p-values less than 0.01 between Parkinson's patients and control subjects according to different genotypes containing FokI-F allele,ApaI-A allele and BsmI-b allele .
Conclusion: The result of this study showed that each of FokI and ApaI recessive alleles can influence serum calcium and parathyroid hormone between healthy individuals and Parkinson's patients significantly.
Aref Mohammadipour, Najmeh Ranji, Leila Asadpour,
Volume 20, Issue 5 (8-2017)
Abstract
Abstract
Background: Pseudomonas aeruginosa is an opportunistic nosocomial pathogen that using several classes of antibiotics to treat has been led to the emergence of multiple drug resistance. One of the drug resistance mechanisms in Pseudomonas aeruginosa is overexpression of mexXY-oprM efflux pump system. Silybin as main flavonolignan of silymarin extracted from Silybum marianum is a hepatoprotective agent that its anti-bacterial properties was studied, recently. In this study, the effect of combination of silybin and ciprofloxacin on oprM gene expression in clinical isolates of Pseudomonas aeruginosa was evaluated.
Materials and Methods: In this study, seven ciprofloxacin resistant isolates of Pseudomonas aeruginosa were treated by ciprofloxacin (1/2MIC) only (control sample) and in the combination with silybin-encapsulated micelle (nanoparticles) (test sample). After 24h, RNA extraction and cDNA synthesis were performed in silybin treated and un-treated cells and oprM gene expression was quantitatively investigated by realtime PCR method.
Results: Results of this study showed that a silybin encapsulated in nanoparticles (400µg/ml) induces death up to 50% in resistant isolates treated by ciprofloxacin (1/2MIC) during 24h. Also, quantitative Real-Time PCR analysis revealed that silybin encapsulated in nanoparticles decreases the expression of oprM gene compared to silybin untreated cells.
Conclusion: It seems that Decrease of oprM expression in resistant isolates lead to decrease of mexAB-oprM and mexXY-oprM in cell surface, subsequently decrease of antibiotic withdrawal to extracellular environment and increase of sensitivity to antibiotics.
Esmail Asadpour, Saeed Veisi,
Volume 20, Issue 7 (10-2017)
Abstract
Abstract
Background: The aim of this study was to investigate the effectiveness of emotionally focused couple therapy on sexual self-esteem and sexual function in women with multiple sclerosis in the city of Tehran.
Materials and Methods: This was a quasi experimental study with pretest-posttest, a month follow-up and control group. The statistical population included women with multiple sclerosis in the city of Tehran. 30 patients with the age range of 20-50 years old were chosen as samples and randomly were divided in 2 groups of individuals as control and emotionally focused therapy. The experimental groups practiced the emotionally focused therapy for a month, 9 step and 60 minutes in each session. Then, post-test was performed for them. For data collecting, the sexual self-esteem and sexual function questionnaires were used. Data analysis was done by using multivariate analysis of covariance by SPSS 23 software at a significant level less than 0.05.
Results: Statistical data analysis illustrated that the amounts of sexual self-esteem of life after emotionally focused couple therapy intervention were significantly increased in experimental than control group (p<0.05). Also, of emotionally focused therapy was significant increase on sexual function of women with multiple sclerosis (p<0.05).
Conclusion: According to the findings of this study, emotionally focused couple therapy has affected on sexual self-esteem and sexual function in women with multiple sclerosis Thus, emotionally focused couple therapy can be a good approach to improve the sexual problems in women with multiple sclerosis.
Javad Sohrabi Asadabad, Zohreh Ghotbeddin, Mohammad Reza Tabandeh,
Volume 20, Issue 9 (12-2017)
Abstract
Abstract
Background: A lot of studies indicate that cancer chemotherapy drugs such as doxorubicin results in memory impairment. On the other hand, crocin as the chemical constituent isolated from the Saffron is effective on memory and motor enhancement. So, in this work, we have studied the co-administration effect of crocin and doxorubicin on avoidance memory and motor activity in adult male rat.
Material and Methods: In this study, 50 male rats were divided into 5 groups: control, sham, doxorubicin, crocin and treated rats with coadministration of doxorubicin and crocin. In crocin group, crocin injected 30mg/kg for 21 days and each rat in the chemotherapy group was treated once a week for 3 weeks with doxorubicin (5mg/kg). Treatment group, received doxorubicin and crocin at the same time. Sham groups administrated with saline. All drugs were injected intraperitoneally. After these procure passive avoidance memory, balance and exploratory behaviors were assessed respectively by shuttle box, rotarod and open field instruments.
Results: Memory in rats which have consumed doxorubicin significantly was decreased compared to other groups (p<0.001). Crocin treatment improved memory impairment following doxorubicin injection (p<0.001). Motor activity in open field and rotarod tests in treatment group showed significantly increased compared to doxorubicin group (p<0.001).
Conclusion: Crocin consumption beside of anticancer drugs such as doxorubicin has protective effect on the bad effects of chemotherapy drugs on memory and movement.
Alireza Khodavandi, Fahimeh Alizadeh, Nedasadat Marashi,
Volume 20, Issue 11 (2-2018)
Abstract
Abstract
Background: Biofilm formation represents one of the major virulence factors of Candida albican. However, the number of antifungal drugs is limited for the treatment of candidiasis. Combination therapy is one of the most frequently used techniques to alleviate this problem. In this study, we aimed to evaluate the antifungal activity of fluconazole and terbinafine alone and in combination on C. albicans biofilm inhibition.
Materials and Methods: In this cross- sectional study, 10 clinical isolates of C. albicans were identified from the immunocompromised patients. Antifungal susceptibilities were performed using the CLSI standard reference method. The crystal violet colorimetric method, direct microscopic observation and expression of HWP1 gene at different concentrations based on MICs were carried out to investigate inhibition of biofilm formation in C. albicans treated alone and in combination with fluconazole and terbinafine.
Results: The data indicated that combination of fluconazole with terbinafine exerted synergistic effects with fractional inhibitory concentration index ranged from 0.375 to 1.5. The combination of fluconazole with terbinafine reduced the number of yeast form and inhibited the biofilm formation. Finally, the expression level of HWP1 was down regulated (p< 0.05).
Conclusion: These results suggest the possibility of fluconazole/ terbinafine to treat candidiasis with a higher efficiency. In addition, HWP1 gene could be probable target in synergistic interaction of fluconazole/ terbinafine against C. albicans biofilm.
Bozorgmehr Imani Pirsaraei, Najmeh Ranji, Leila Asadpour,
Volume 21, Issue 2 (5-2018)
Abstract
Abstract
Background: Pseudomonas aeruginosa is an opportunistic gram-negative bacterium that is a major cause of nosocomial infections such as severe burns. Curcumin is the main component of turmeric (Curcuma longa) that has anti-cancer and anti-inflammatory effects. The aim of this study was to evaluate antibacterial effect of curcumin in ciprofloxacin resistant isolates of Pseudomonas aeruginosa through mexC and mexD gene expression.
Materials and Methods: In this descriptive-analytical study, Pseudomonas aeruginosa strains were obtained from hospitals and laboratories in Guilan province. After disc difusion and MIC tests, four ciprofloxacin resistant strains of Pseudomonas aeruginosa were treated by ciprofloxacin (1/2MIC) only (control sample) and in the combination with curcumin encapsulated in micelle nanoparticles (test sample). After 24h, RNA extraction and cDNA synthesis was performed. Then, the expression of mexC and mexD genes was evaluated quantitatively by Real-time PCR method in curcumin treated and un-treated cells
Results: This study showed that combination of ciprofloxacin (1/2 MIC) with curcumin encapsulated in micelle nanoparticles led to approximately 50% of growth inhibition in Pseudomonas aeruginosa. In treated cells with curcumin and ciprofloxacin compared to treated cells with ciprofloxacin alone, mexC and mexD genes were significantly (p<0.05) downregulated >0.65 fold in three isolates and >0.1 fold in four isolates, respectively.
Conclusion: Our results suggested that curcumin encapsulated in micelle nanoparticles combined with 1/2 MIC value of ciprofloxacin inhibits the growth of Pseudomonas aeruginosa through reducing mexC and mexD expression.
Mis Mahnaz Najafi, Mohammad Reza Asad, Mostafa Rahimi, Rahman Souri, Ali Ahvar,
Volume 21, Issue 4 (8-2018)
Abstract
Background and Aim: The aim of study was to investigate the effect of 8 weeks of moderate-intensity continuous training (MICT) and high intensity interval training (HIIT) on the GLUT4 gene expression in skeletal muscle of obese male rats.
Materials and Methods: Twenty-eight male Wistar rats with 150 ± 25 grams body weight were divided into base control (n = 6), obese control (n = 6), MICT (n = 8), and (n = 8) HIIT groups as study sample. At the beginning of the study, base control group rats were sacrificed. In other groups, rats underwent high fat regime diet for 8 weeks. After one week of familiarization, rats in training groups run on treadmill for 8 weeks and 5 days per week. Forty-eight hours after the last session, the rats were scarified and gastrocnemius muscle tissue sample were removed. GLUT4 gene expression was measured by Real-Time PCR methods.
Findings: Results indicated that high fat diet for 8 weeks significantly increased rats body weight (p= 0.001), whereas both of MICT and HIIT training significantly decreased body weight (p= 0.001). Moreover, the MICT and HIIT had significant increase in GLUT4 gene expression in gastrocnemius muscle of obese rats (p = 0.001). Although, there were no differences between two experimental groups (p = 0.99).
Conclusion: According to the results, the MICT and HIIT increase gene expression of GLUT4 in mixed muscle fibers in obese rats. |
Esmail Asadpour, Saied Veisi,
Volume 21, Issue 7 (2-2019)
Abstract
Background and Aim: Marital and sexual problems of chronic patients, especially diabetes, that often are ignored, need special attention to proper treatment. The aim of this study was to investigate the effectiveness of acceptance and commitment therapy on marital satisfaction, sexual self-esteem and burnout martial among women with type 2 diabetes.
Materials and Methods: This study was a quasi-experimental research design with pretest-posttest and control group. The statistical population included women with type 2 diabetes in the city of Tehran. 30 patients were chosen as samples and randomly were divided into two groups of individuals as control and experimental. The experimental groups practiced the acceptance and commitment therapy 8 sessions and 60 minutes in each session. Then, post-test was performed for them. For data collecting, the marital satisfaction scale of Enrich (1993), sexual self-esteem scale of Doyle, Zeanah and Schwarz (1996) and burnout martial questionnaire of Pines (1993) were used. Data analysis was done by using multivariate analysis of multi covariance.
Ethical Considerations: This study with research ethics code IR.KHU.REC.1397.5 has been approved by research ethics committee at Kharazmi university of Tehran, Iran.
Findings: Results illustrated that after acceptance and commitment therapy, amounts of marital satisfaction and sexual self-esteem were significantly increased in experimental than control group (p<0.05). Also, acceptance and commitment therapy decreased burnout martial among women with type 2 diabetes significantly (p<0.05).
Conclusion: Acceptance and commitment therapy can be used as an effective psychological intervention on marital satisfaction, sexual self-esteem and burnout martial among women with type 2 diabetes.
Mohammad Reza Asad, Shokoufeh Kheradmand, Narges Kheradmand,
Volume 22, Issue 5 (11-2019)
Abstract
Background and Aim Exercise improves sensitivity to insulin by reducing some adipokines including chemerin. However, the effect of endurance exercise and high-intensity intermittent exercise on chemerin level and insulin resistance in obese male rats is still unknown.
Methods & Materials In this study 27 male rats were randomly divided into 4 groups of sedentary control (n=6), endurance exercise (n=8), high-intensity intermittent exercise (n=8) and untreated obese control (n=5). Both endurance and high intensity intermittent exercise groups performed exercises for 8 weeks. The plasma level of chemerin was measured by ELISA method.
Ethical Considerations ???
Results The chemerin level was significantly reduced in the endurance group compared to the untreated obese control group (P<0.01), but it had no significant change compared to the sedentary control group. Moreover, the plasma level of chemerin in the high-intensity intermittent group was not significantly different from that of untreated obese control group and sedentary control group (P>0.05) and chemerin level reduction in the endurance group was significant compared to the high-intensity intermittent group (P<0.05). Furthermore, there was no significant difference in the insulin resistance reported in high-intensity intermittent group compared to that of endurance group (P>0.05), but its difference in high-intensity intermittent and endurance training groups was significant compared to that of untreated obese control and sedentary control groups (P<0.05).
Conclusion Both endurance and high-intensity intermittent exercises can reduce insulin resistance which can be due to the reduction of chemerin plasma level and weight loss.
Mojtaba Asad Samani, Maryam Peymani,
Volume 24, Issue 2 (June & July 2021)
Abstract
Background and Aim: The protein encoded by the SGO1 gene is a member of the shugoshin family of proteins and protects the centromere during mitosis. lncRNAs are non-coding RNA with 200 nucleotides lengths, i.e., involved in regulating gene expression. The current study aimed to evaluate the expression of SGO1 and SGO1-AS1 in different stages of disease progression; we also compared their expression pattern in tumor tissues with healthy tissues in colorectal cancer patients.
Methods & Materials: In total, 40 tissue samples of patients with colorectal cancer were reported according to the examination and criteria with the approval of a pathologist. Besides, 40 normal tissues were sampled from a completely healthy part of the intestine of the same patients. After RNA extraction and cDNA synthesis, the Real-time RT-PCR technique was used to evaluate the expression of the desired genes in the study groups. ROC curve analysis was also used to determine the ability of each selected gene to diagnose the disease.
Ethical Considerations: This study was approved by the Ethics Committee of Shahrekord Azad University (Code: IR.IAU.SHKREC.1398.020).
Results The obtained data suggested that SGO1 significantly decreased in the colorectal cancer tumor samples (P<0.001) and SGO1-AS1 LncRNA significantly increased expression, compared to adjacent healthy tissues. Additionally, in the age group of below 60 years, compared to the age group of over 60 years, SGO1 expression increased and SGO1-AS1 expression decreased. Based on the AUC obtained from the ROC diagram, it was found that the SGO1 gene with AUC=0.8041 and SGO1-AS1 with AUC=0.6364 could significantly distinguish a healthy population from patients with colorectal cancer.
Conclusion: According to the collected results, SGO1 -AS1 and SGO1 were significantly reduced and increased in tumor tissue, respectively; however, only the SGO1 gene was introduced as a good marker for diagnosing colorectal cancer.
Nasin Asadi, Amineh Ahmadi, Asadollah Abbasi,
Volume 25, Issue 1 (April & May- 2022)
Abstract
Background and Aim The occupational environment, the type of work overload or underload, physical risks, how individuals adapt to the workplace, and face the family - work constitute the sources of stress or occupational distress. Job stress emerges as the duties and tasks assigned to people are more than their abilities. This study aimed to investigate the relationship between stress management training and work ethics of employees.
Methods & Materials This research was an applied study in terms of purpose, a mixed exploratory (qualitative and quantitative) study in terms of data, a content analysis (qualitative stage) and cross-sectional survey (quantitative stage) in term of conduct. The study population in the qualitative section comprised experts (Experts in Psychology, Educational Management, and Social Medicine) according to the inclusion and exclusion criteria. In the qualitative section, the study population included experts and managers who had received stress management training. Sample size and sampling method in qualitative part was based on the principle of theoretical saturation, resulting in 12 people using purposive sampling method. In the quantitative part, the sampling was based on the Cochran’s formula, resulting in 220 people who were selected using cluster sampling method.
Ethical Considerations This barcode research was presented to the Ethics Committee and registered in the system (IR.IAU.TNB.REC.1400.121).
Results The results showed that to deal with stressful situations, three basic strategies of event-focused coping, anxiety-focused coping, and avoidance-focused coping strategies can be used for stress management training models. Overall, the components presented for coping with stressful conditions have the necessary and appropriate validities. All three main coping strategies (event-focused, anxiety-focused, and refusal-focused) are suitable for explaining and fitting. So, they all are reliable and confirmed in the current research questionnaire of coping with stress.
Conclusion Strategies for coping with stressful situations have a different effect on people’s professional work ethics so that the event-focused coping strategy has a significant positive relationship with professional ethics. On the other hand, the anxiety-focused and avoidance-focused coping strategies have negative and decreasing relationship with professional ethics. The more emphasis on the event-focused coping strategy, the more would be the professional ethics of individuals and the more emphasis on the anxiety-focused and avoidance-focused coping strategies, the less would be the professional ethics of individuals..
